LOCUS       sequence-37663-e         978 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 978)
  AUTHORS   Bullard JM, Cai YC, Spremulli LL
  TITLE     Expression and characterization of the human mitochondrial 
            leucyl-tRNA synthetase.
  JOURNAL   Biochim Biophys Acta. 2000 Feb 29. 1490(3):245-58.
  PUBMED    10684970
REFERENCE   2  (bases 1 to 978)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biochim 
            Biophys Acta. 2000 Feb 29. 1490(3):245-58."
COMMENT     Sequence Label: pET21c(+)-mtLeuRS
FEATURES             Location/Qualifiers
     source          1..978
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(24..41)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gggcttgtng cagccggatc tcagtggtgg tggtggtggt gctcgacatc ttgcaccagg
       61 aagttgatga gggcagttct cggggaaagg aaggacttct tgatgcttcg tccttgcaaa
      121 agcctgacac ccagctcgct ttgaagaaca aattcgtgga ctttgtcctg gtcccgggca
      181 acttgttggg gcacaggaat tttgccacaa gctttattgt tgatcagaac tgccatctgg
      241 acaacctcag gctgctgcag gaactccggg tccacagcag gccatgcctg gagcagcaca
      301 ctggcatccc aagtgtagtg ggcacagagc ttcctcggca ccagcgccag gcctgcccag
      361 atctctgagg ttacatgagg ggccagtgga gcagccatta ccatcagggc acacaaagca
      421 tcctcaaact cggggctgtg gagaatgacg ctctgagagg cttgcgagag tgcattgctg
      481 agtcccatca gctgagaaat tgcagaattc agtgagaagt cctctgtgaa atgggtggtc
      541 acctgagaga tgacggagtt cttgtactcc cagagcttcc tggcctcagc tttctccttg
      601 ttactcagca gctgaggctg gggagacttc ccagaagccc tggcctcaat aaaccgagtt
      661 gtcaaggtcc acagtcgttg ttgccatctc agcaccccag ggagagcatc agttttcaca
      721 tcccacaaga tatccttctc aggaggggca gcaaaaagga tgtagagccg aatcgtgtcg
      781 atcccatact gctccacaac ttcctctggg tccaccccgt tgtgtttgga cttactcatc
      841 ttctcccacg tcacctctaa cttctctttc gtttttgcat gaacaggaac ggaaacctgt
      901 gagatccact tcctctctct gtagatactg tccagatggt angcggaatt canccctaag
      961 tgccctaaag ccntactt
//
