LOCUS       sequence-376666-       10446 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express Cas9/-Eco1 RT fusion, using linker2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10446)
  AUTHORS   Lopez SC, Crawford KD, Lear SK, Bhattarai-Kline S, Shipman SL
  TITLE     Precise genome editing across kingdoms of life using retron-derived 
            DNA.
  JOURNAL   Nat Chem Biol. 2022 Feb;18(2):199-206. doi: 
            10.1038/s41589-021-00927-y. Epub 2021 Dec 23.
  PUBMED    34949838
REFERENCE   2  (bases 1 to 10446)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41589-021-00927-y"; journalName: "Nat Chem Biol"; date: 
            "2022-02"; volume: "18"; issue: "2"; pages: "199-206"
COMMENT     Sequence Label: pSCL.95
FEATURES             Location/Qualifiers
     source          1..10446
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1012..1032
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(1064..1082)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     terminator      1094..1283
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(1322..1342)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(1322..1340)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1361..1377)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1361..1377)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1374..1396)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1385..1401
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1409..1415,1416..1433,1434..1439))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1454..1475
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1592..1609)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1763..2351)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1843..1862)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2522..3313,3314..3382))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3145..3164
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3383..3487)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3555..3573
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(3611..3633)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3733..3752
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        3899..4086
                     /gene="S. cerevisiae HIS3"
                     /label=HIS3 promoter
     CDS             4087..4749
                     /codon_start=1
                     /gene="S. cerevisiae HIS3"
                     /product="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
                     /label=HIS3
                     /note="yeast auxotrophic marker"
                     /translation="MTEQKALVKRITNETKIQIAISLKGGPLAIEHSIFPEKEAEAVAE
                     QATQSQVINVHTGIGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHTTEDCGIALGQAF
                     KEALGAVRGVKRFGSGFAPLDEALSRAVVDLSNRPYAVVELGLQREKVGDLSCEMIPHF
                     LESFAEASRITLHVDCLRGKNDHHRSESAFKALAVAIREATSPNGTNDVPSTKGVLM"
     rep_origin      complement(5009..5464)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5146..5167)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5358..5377
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     CDS             complement(5546..5566)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     promoter        5788..6229
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     protein_bind    5788..5905
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     6200..6223
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     CDS             6277..6297
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6298..10398
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             6298..10398
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             6298..10398
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
ORIGIN
        1 agcgagtcag caacaccaga gagcagtggc ggcagcagcg gcggcagcag caagtccgct
       61 gaatacttga acaccttcag attgagaaat ttgggtttgc cagtcatgaa caacttgcac
      121 gatatgtcta aggctaccag aatttctgtt gaaaccttga gattattgat ctacaccgcc
      181 gatttcagat acagaatcta cactgttgaa aagaagggtc cagaaaagag aatgagaact
      241 atctaccaac catccagaga attgaaggct ttacaaggtt gggttttgag aaacatcttg
      301 gacaagttat cctcctcccc attttctatc ggttttgaaa agcaccaatc catcttgaac
      361 aatgccactc cacatattgg tgccaacttc attttgaaca tcgacttgga agatttcttc
      421 ccatctttga ctgctaacaa ggttttcggt gttttccatt ctttgggtta caacagattg
      481 atctcctctg ttttgaccaa gatttgctgc tacaagaact tgttgccaca aggtgctcca
      541 tcttctccaa aattggctaa cttgatttgc tccaagttgg actacagaat tcaaggttat
      601 gctggttcta gaggtttgat atacactaga tacgctgatg acttgacctt gtctgctcaa
      661 tctatgaaga aagttgttaa ggccagagac ttcttgttct ccattattcc atcagaaggt
      721 ttggtcatca actccaaaaa gacctgtatt tctggtccaa gatcccaaag aaaagttacc
      781 ggtttggtta tctcccaaga aaaggttggt attggtagag aaaagtacaa agaaatcaga
      841 gccaagatcc accatatttt ctgcggtaaa tcctccgaaa tcgaacatgt tagaggttgg
      901 ttgtctttca tcttgtccgt tgattctaag tcccacagaa gattgattac ctacatctct
      961 aagttggaaa agaagtacgg taagaaccca ttgaacaagg ctaagactcc accaaagaag
     1021 aaaagaaagg tctgatctct tctcgagtca tgtaattagt tatgtcacgc ttacattcac
     1081 gccctccccc cacatccgct ctaaccgaaa aggaaggagt tagacaacct gaagtctagg
     1141 tccctattta tttttttata gttatgttag tattaagaac gttatttata tttcaaattt
     1201 ttcttttttt tctgtacaga cgcgtgtacg catgtaacat tatactgaaa accttgcttg
     1261 agaaggtttt gggacgctcg aaggctttaa tttcaccgcg gtggagctcc agcttttgtt
     1321 ccctttagtg agggttaatt gcgcgcttgg cgtaatcatg gtcatagctg tttcctgtgt
     1381 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
     1441 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
     1501 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
     1561 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     1621 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     1681 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     1741 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     1801 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     1861 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     1921 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     1981 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     2041 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     2101 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     2161 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     2221 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     2281 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     2341 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     2401 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     2461 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     2521 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     2581 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     2641 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     2701 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     2761 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     2821 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     2881 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     2941 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     3001 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     3061 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     3121 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     3181 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     3241 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     3301 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     3361 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     3421 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     3481 ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga
     3541 cattaaccta taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg
     3601 acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg
     3661 atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct
     3721 ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccataaa ttcccgtttt
     3781 aagagcttgg tgagcgctag gagtcactgc caggtatcgt ttgaacacgg cattagtcag
     3841 ggaagtcata acacagtcct ttcccgcaat tttctttttc tattactctt ggcctcctct
     3901 agtacactct atattttttt atgcctcggt aatgattttc attttttttt ttccacctag
     3961 cggatgactc tttttttttc ttagcgattg gcattatcac ataatgaatt atacattata
     4021 taaagtaatg tgatttcttc gaagaatata ctaaaaaatg agcaggcaag ataaacgaag
     4081 gcaaagatga cagagcagaa agccctagta aagcgtatta caaatgaaac caagattcag
     4141 attgcgatct ctttaaaggg tggtccccta gcgatagagc actcgatctt cccagaaaaa
     4201 gaggcagaag cagtagcaga acaggccaca caatcgcaag tgattaacgt ccacacaggt
     4261 atagggtttc tggaccatat gatacatgct ctggccaagc attccggctg gtcgctaatc
     4321 gttgagtgca ttggtgactt acacatagac gaccatcaca ccactgaaga ctgcgggatt
     4381 gctctcggtc aagcttttaa agaggcccta ggggccgtgc gtggagtaaa aaggtttgga
     4441 tcaggatttg cgcctttgga tgaggcactt tccagagcgg tggtagatct ttcgaacagg
     4501 ccgtacgcag ttgtcgaact tggtttgcaa agggagaaag taggagatct ctcttgcgag
     4561 atgatcccgc attttcttga aagctttgca gaggctagca gaattaccct ccacgttgat
     4621 tgtctgcgag gcaagaatga tcatcaccgt agtgagagtg cgttcaaggc tcttgcggtt
     4681 gccataagag aagccacctc gcccaatggt accaacgatg ttccctccac caaaggtgtt
     4741 cttatgtagt gacaccgatt atttaaagct gcagcatacg atatatatac atgtgtatat
     4801 atgtatacct atgaatgtca gtaagtatgt atacgaacag tatgatactg aagatgacaa
     4861 ggtaatgcat cattctatac gtgtcattct gaacgaggcg cgctttcctt ttttcttttt
     4921 gctttttctt tttttttctc ttgaactcga cggatctatg cggtgtgaaa taccgcacag
     4981 atgcgtaagg agaaaatacc gcatcaggaa attgtaagcg ttaatatttt gttaaaattc
     5041 gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc
     5101 ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag
     5161 agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc
     5221 gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa
     5281 gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg
     5341 aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt
     5401 gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc
     5461 gcgtccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt
     5521 cgctattacg ccagtcagcg gacttgacct ttcttttctt ctttggtggc atttatattg
     5581 aattttcaaa aattcttact ttttttttgg atggacgcaa agaagtttaa taatcatatt
     5641 acatggcatt accaccatat acatatccat atctaatctt acttatatgt tgtggaaatg
     5701 taaagagccc cattatctta gcctaaaaaa accttctctt tggaactttc agtaatacgc
     5761 ttaactgctc attgctatat tgaagtacgg attagaagcc gccgagcggg cgacagccct
     5821 ccgacggaag actctcctcc gtgcgtcctc gtcttcaccg gtcgcgttcc tgaaacgcag
     5881 atgtgcctcg cgccgcactg ctccgaacaa taaagattct acaatactag cttttatggt
     5941 tatgaagagg aaaaattggc agtaacctgg ccccacaaac cttcaaatta acgaatcaaa
     6001 ttaacaacca taggatgata atgcgattag ttttttagcc ttatttctgg ggtaattaat
     6061 cagcgaagcg atgatttttg atctattaac agatatataa atggaaaagc tgcataacca
     6121 ctttaactaa tactttcaac attttcagtt tgtattactt cttattcaaa tgtcataaaa
     6181 gtatcaacaa aaaattgtta atatacctct atactttaac gtcaaggaga aaaaacaact
     6241 agtggatccc ccgggaaaaa aatgagcagg gctgacccca agaagaagag gaaggtggac
     6301 aagaagtact ccattgggct cgatatcggc acaaacagcg tcggttgggc cgtcattacg
     6361 gacgagtaca aggtgccgag caaaaaattc aaagttctgg gcaataccga tcgccacagc
     6421 ataaagaaga acctcattgg cgccctcctg ttcgactccg gggagacggc cgaagccacg
     6481 cggctcaaaa gaacagcacg gcgcagatat acccgcagaa agaatcggat ctgctacctg
     6541 caggagatct ttagtaatga gatggctaag gtggatgact ctttcttcca taggctggag
     6601 gagtcctttt tggtggagga ggataaaaag cacgagcgcc acccaatctt tggcaatatc
     6661 gtggacgagg tggcgtacca tgaaaagtac ccaaccatat atcatctgag gaagaagctt
     6721 gtagacagta ctgataaggc tgacttgcgg ttgatctatc tcgcgctggc gcatatgatc
     6781 aaatttcggg gacacttcct catcgagggg gacctgaacc cagacaacag cgatgtcgac
     6841 aaactcttta tccaactggt tcagacttac aatcagcttt tcgaagagaa cccgatcaac
     6901 gcatccggag ttgacgccaa agcaatcctg agcgctaggc tgtccaaatc ccggcggctc
     6961 gaaaacctca tcgcacagct ccctggggag aagaagaacg gcctgtttgg taatcttatc
     7021 gccctgtcac tcgggctgac ccccaacttt aaatctaact tcgacctggc cgaagatgcc
     7081 aagcttcaac tgagcaaaga cacctacgat gatgatctcg acaatctgct ggcccagatc
     7141 ggcgaccagt acgcagacct ttttttggcg gcaaagaacc tgtcagacgc cattctgctg
     7201 agtgatattc tgcgagtgaa cacggagatc accaaagctc cgctgagcgc tagtatgatc
     7261 aagcgctatg atgagcacca ccaagacttg actttgctga aggcccttgt cagacagcaa
     7321 ctgcctgaga agtacaagga aattttcttc gatcagtcta aaaatggcta cgccggatac
     7381 attgacggcg gagcaagcca ggaggaattt tacaaattta ttaagcccat cttggaaaaa
     7441 atggacggca ccgaggagct gctggtaaag cttaacagag aagatctgtt gcgcaaacag
     7501 cgcactttcg acaatggaag catcccccac cagattcacc tgggcgaact gcacgctatc
     7561 ctcaggcggc aagaggattt ctaccccttt ttgaaagata acagggaaaa gattgagaaa
     7621 atcctcacat ttcggatacc ctactatgta ggccccctcg cccggggaaa ttccagattc
     7681 gcgtggatga ctcgcaaatc agaagagacc atcactccct ggaacttcga ggaagtcgtg
     7741 gataaggggg cctctgccca gtccttcatc gaaaggatga ctaactttga taaaaatctg
     7801 cctaacgaaa aggtgcttcc taaacactct ctgctgtacg agtacttcac agtttataac
     7861 gagctcacca aggtcaaata cgtcacagaa gggatgagaa agccagcatt cctgtctgga
     7921 gagcagaaga aagctatcgt ggacctcctc ttcaagacga accggaaagt taccgtgaaa
     7981 cagctcaaag aagactattt caaaaagatt gaatgtttcg actctgttga aatcagcgga
     8041 gtggaggatc gcttcaacgc atccctggga acgtatcacg atctcctgaa aatcattaaa
     8101 gacaaggact tcctggacaa tgaggagaac gaggacattc ttgaggacat tgtcctcacc
     8161 cttacgttgt ttgaagatag ggagatgatt gaagaacgct tgaaaactta cgctcatctc
     8221 ttcgacgaca aagtcatgaa acagctcaag aggcgccgat atacaggatg ggggcggctg
     8281 tcaagaaaac tgatcaatgg gatccgagac aagcagagtg gaaagacaat cctggatttt
     8341 cttaagtccg atggatttgc caaccggaac ttcatgcagt tgatccatga tgactctctc
     8401 acctttaagg aggacatcca gaaagcacaa gtttctggcc agggggacag tcttcacgag
     8461 cacatcgcta atcttgcagg tagcccagct atcaaaaagg gaatactgca gaccgttaag
     8521 gtcgtggatg aactcgtcaa agtaatggga aggcataagc ccgagaatat cgttatcgag
     8581 atggcccgag agaaccaaac tacccagaag ggacagaaga acagtaggga aaggatgaag
     8641 aggattgaag agggtataaa agaactgggg tcccaaatcc ttaaggaaca cccagttgaa
     8701 aacacccagc ttcagaatga gaagctctac ctgtactacc tgcagaacgg cagggacatg
     8761 tacgtggatc aggaactgga catcaatcgg ctctccgact acgacgtgga tcatatcgtg
     8821 ccccagtctt ttctcaaaga tgattctatt gataataaag tgttgacaag atccgataaa
     8881 aatagaggga agagtgataa cgtcccctca gaagaagttg tcaagaaaat gaaaaattat
     8941 tggcggcagc tgctgaacgc caaactgatc acacaacgga agttcgataa tctgactaag
     9001 gctgaacgag gtggcctgtc tgagttggat aaagccggct tcatcaaaag gcagcttgtt
     9061 gagacacgcc agatcaccaa gcacgtggcc caaattctcg attcacgcat gaacaccaag
     9121 tacgatgaaa atgacaaact gattcgagag gtgaaagtta ttactctgaa gtctaagctg
     9181 gtctcagatt tcagaaagga ctttcagttt tataaggtga gagagatcaa caattaccac
     9241 catgcgcatg atgcctacct gaatgcagtg gtaggcactg cacttatcaa aaaatatccc
     9301 aagcttgaat ctgaatttgt ttacggagac tataaagtgt acgatgttag gaaaatgatc
     9361 gcaaagtctg agcaggaaat aggcaaggcc accgctaagt acttctttta cagcaatatt
     9421 atgaattttt tcaagaccga gattacactg gccaatggag agattcggaa gcgaccactt
     9481 atcgaaacaa acggagaaac aggagaaatc gtgtgggaca agggtaggga tttcgcgaca
     9541 gtccggaagg tcctgtccat gccgcaggtg aacatcgtta aaaagaccga agtacagacc
     9601 ggaggcttct ccaaggaaag tatcctcccg aaaaggaaca gcgacaagct gatcgcacgc
     9661 aaaaaagatt gggaccccaa gaaatacggc ggattcgatt ctcctacagt cgcttacagt
     9721 gtactggttg tggccaaagt ggagaaaggg aagtctaaaa aactcaaaag cgtcaaggaa
     9781 ctgctgggca tcacaatcat ggagcgatca agcttcgaaa aaaaccccat cgactttctc
     9841 gaggcgaaag gatataaaga ggtcaaaaaa gacctcatca ttaagcttcc caagtactct
     9901 ctctttgagc ttgaaaacgg ccggaaacga atgctcgcta gtgcgggcga gctgcagaaa
     9961 ggtaacgagc tggcactgcc ctctaaatac gttaatttct tgtatctggc cagccactat
    10021 gaaaagctca aagggtctcc cgaagataat gagcagaagc agctgttcgt ggaacaacac
    10081 aaacactacc ttgatgagat catcgagcaa ataagcgaat tctccaaaag agtgatcctc
    10141 gccgacgcta acctcgataa ggtgctttct gcttacaata agcacaggga taagcccatc
    10201 agggagcagg cagaaaacat tatccacttg tttactctga ccaacttggg cgcgcctgca
    10261 gccttcaagt acttcgacac caccatagac agaaagcggt acacctctac aaaggaggtc
    10321 ctggacgcca cactgattca tcagtcaatt acggggctct atgaaacaag aatcgacctc
    10381 tctcagctcg gtggagactc tggaggatct agcggaggat cctctggcag cgagacacca
    10441 ggaaca
//
