LOCUS       sequence-376672-       10407 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express Cas9/-Eco1 RT fusion, using linker1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10407)
  AUTHORS   Lopez SC, Crawford KD, Lear SK, Bhattarai-Kline S, Shipman SL
  TITLE     Precise genome editing across kingdoms of life using retron-derived 
            DNA.
  JOURNAL   Nat Chem Biol. 2022 Feb;18(2):199-206. doi: 
            10.1038/s41589-021-00927-y. Epub 2021 Dec 23.
  PUBMED    34949838
REFERENCE   2  (bases 1 to 10407)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41589-021-00927-y"; journalName: "Nat Chem Biol"; date: 
            "2022-02"; volume: "18"; issue: "2"; pages: "199-206"
COMMENT     Sequence Label: pSCL.72
FEATURES             Location/Qualifiers
     source          1..10407
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(12..32)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     promoter        254..695
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     protein_bind    254..371
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     666..689
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     CDS             743..763
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             764..4864
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             764..4864
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             764..4864
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             5885..5905
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(5937..5955)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     terminator      5967..6156
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(6195..6215)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(6195..6213)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6234..6250)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6234..6250)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6247..6269)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6258..6274
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6282..6288,6289..6306,6307..6312))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6327..6348
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6465..6482)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6636..7224)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6716..6735)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7395..8186,8187..8255))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8018..8037
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8256..8360)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8428..8446
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8484..8506)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8606..8625
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        8772..8959
                     /gene="S. cerevisiae HIS3"
                     /label=HIS3 promoter
     CDS             8960..9622
                     /codon_start=1
                     /gene="S. cerevisiae HIS3"
                     /product="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
                     /label=HIS3
                     /note="yeast auxotrophic marker"
                     /translation="MTEQKALVKRITNETKIQIAISLKGGPLAIEHSIFPEKEAEAVAE
                     QATQSQVINVHTGIGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHTTEDCGIALGQAF
                     KEALGAVRGVKRFGSGFAPLDEALSRAVVDLSNRPYAVVELGLQREKVGDLSCEMIPHF
                     LESFAEASRITLHVDCLRGKNDHHRSESAFKALAVAIREATSPNGTNDVPSTKGVLM"
     rep_origin      complement(9882..10337)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10019..10040)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     10231..10250
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 tcagcggact tgacctttct tttcttcttt ggtggcattt atattgaatt ttcaaaaatt
       61 cttacttttt ttttggatgg acgcaaagaa gtttaataat catattacat ggcattacca
      121 ccatatacat atccatatct aatcttactt atatgttgtg gaaatgtaaa gagccccatt
      181 atcttagcct aaaaaaacct tctctttgga actttcagta atacgcttaa ctgctcattg
      241 ctatattgaa gtacggatta gaagccgccg agcgggcgac agccctccga cggaagactc
      301 tcctccgtgc gtcctcgtct tcaccggtcg cgttcctgaa acgcagatgt gcctcgcgcc
      361 gcactgctcc gaacaataaa gattctacaa tactagcttt tatggttatg aagaggaaaa
      421 attggcagta acctggcccc acaaaccttc aaattaacga atcaaattaa caaccatagg
      481 atgataatgc gattagtttt ttagccttat ttctggggta attaatcagc gaagcgatga
      541 tttttgatct attaacagat atataaatgg aaaagctgca taaccacttt aactaatact
      601 ttcaacattt tcagtttgta ttacttctta ttcaaatgtc ataaaagtat caacaaaaaa
      661 ttgttaatat acctctatac tttaacgtca aggagaaaaa acaactagtg gatcccccgg
      721 gaaaaaaatg agcagggctg accccaagaa gaagaggaag gtggacaaga agtactccat
      781 tgggctcgat atcggcacaa acagcgtcgg ttgggccgtc attacggacg agtacaaggt
      841 gccgagcaaa aaattcaaag ttctgggcaa taccgatcgc cacagcataa agaagaacct
      901 cattggcgcc ctcctgttcg actccgggga gacggccgaa gccacgcggc tcaaaagaac
      961 agcacggcgc agatataccc gcagaaagaa tcggatctgc tacctgcagg agatctttag
     1021 taatgagatg gctaaggtgg atgactcttt cttccatagg ctggaggagt cctttttggt
     1081 ggaggaggat aaaaagcacg agcgccaccc aatctttggc aatatcgtgg acgaggtggc
     1141 gtaccatgaa aagtacccaa ccatatatca tctgaggaag aagcttgtag acagtactga
     1201 taaggctgac ttgcggttga tctatctcgc gctggcgcat atgatcaaat ttcggggaca
     1261 cttcctcatc gagggggacc tgaacccaga caacagcgat gtcgacaaac tctttatcca
     1321 actggttcag acttacaatc agcttttcga agagaacccg atcaacgcat ccggagttga
     1381 cgccaaagca atcctgagcg ctaggctgtc caaatcccgg cggctcgaaa acctcatcgc
     1441 acagctccct ggggagaaga agaacggcct gtttggtaat cttatcgccc tgtcactcgg
     1501 gctgaccccc aactttaaat ctaacttcga cctggccgaa gatgccaagc ttcaactgag
     1561 caaagacacc tacgatgatg atctcgacaa tctgctggcc cagatcggcg accagtacgc
     1621 agaccttttt ttggcggcaa agaacctgtc agacgccatt ctgctgagtg atattctgcg
     1681 agtgaacacg gagatcacca aagctccgct gagcgctagt atgatcaagc gctatgatga
     1741 gcaccaccaa gacttgactt tgctgaaggc ccttgtcaga cagcaactgc ctgagaagta
     1801 caaggaaatt ttcttcgatc agtctaaaaa tggctacgcc ggatacattg acggcggagc
     1861 aagccaggag gaattttaca aatttattaa gcccatcttg gaaaaaatgg acggcaccga
     1921 ggagctgctg gtaaagctta acagagaaga tctgttgcgc aaacagcgca ctttcgacaa
     1981 tggaagcatc ccccaccaga ttcacctggg cgaactgcac gctatcctca ggcggcaaga
     2041 ggatttctac ccctttttga aagataacag ggaaaagatt gagaaaatcc tcacatttcg
     2101 gataccctac tatgtaggcc ccctcgcccg gggaaattcc agattcgcgt ggatgactcg
     2161 caaatcagaa gagaccatca ctccctggaa cttcgaggaa gtcgtggata agggggcctc
     2221 tgcccagtcc ttcatcgaaa ggatgactaa ctttgataaa aatctgccta acgaaaaggt
     2281 gcttcctaaa cactctctgc tgtacgagta cttcacagtt tataacgagc tcaccaaggt
     2341 caaatacgtc acagaaggga tgagaaagcc agcattcctg tctggagagc agaagaaagc
     2401 tatcgtggac ctcctcttca agacgaaccg gaaagttacc gtgaaacagc tcaaagaaga
     2461 ctatttcaaa aagattgaat gtttcgactc tgttgaaatc agcggagtgg aggatcgctt
     2521 caacgcatcc ctgggaacgt atcacgatct cctgaaaatc attaaagaca aggacttcct
     2581 ggacaatgag gagaacgagg acattcttga ggacattgtc ctcaccctta cgttgtttga
     2641 agatagggag atgattgaag aacgcttgaa aacttacgct catctcttcg acgacaaagt
     2701 catgaaacag ctcaagaggc gccgatatac aggatggggg cggctgtcaa gaaaactgat
     2761 caatgggatc cgagacaagc agagtggaaa gacaatcctg gattttctta agtccgatgg
     2821 atttgccaac cggaacttca tgcagttgat ccatgatgac tctctcacct ttaaggagga
     2881 catccagaaa gcacaagttt ctggccaggg ggacagtctt cacgagcaca tcgctaatct
     2941 tgcaggtagc ccagctatca aaaagggaat actgcagacc gttaaggtcg tggatgaact
     3001 cgtcaaagta atgggaaggc ataagcccga gaatatcgtt atcgagatgg cccgagagaa
     3061 ccaaactacc cagaagggac agaagaacag tagggaaagg atgaagagga ttgaagaggg
     3121 tataaaagaa ctggggtccc aaatccttaa ggaacaccca gttgaaaaca cccagcttca
     3181 gaatgagaag ctctacctgt actacctgca gaacggcagg gacatgtacg tggatcagga
     3241 actggacatc aatcggctct ccgactacga cgtggatcat atcgtgcccc agtcttttct
     3301 caaagatgat tctattgata ataaagtgtt gacaagatcc gataaaaata gagggaagag
     3361 tgataacgtc ccctcagaag aagttgtcaa gaaaatgaaa aattattggc ggcagctgct
     3421 gaacgccaaa ctgatcacac aacggaagtt cgataatctg actaaggctg aacgaggtgg
     3481 cctgtctgag ttggataaag ccggcttcat caaaaggcag cttgttgaga cacgccagat
     3541 caccaagcac gtggcccaaa ttctcgattc acgcatgaac accaagtacg atgaaaatga
     3601 caaactgatt cgagaggtga aagttattac tctgaagtct aagctggtct cagatttcag
     3661 aaaggacttt cagttttata aggtgagaga gatcaacaat taccaccatg cgcatgatgc
     3721 ctacctgaat gcagtggtag gcactgcact tatcaaaaaa tatcccaagc ttgaatctga
     3781 atttgtttac ggagactata aagtgtacga tgttaggaaa atgatcgcaa agtctgagca
     3841 ggaaataggc aaggccaccg ctaagtactt cttttacagc aatattatga attttttcaa
     3901 gaccgagatt acactggcca atggagagat tcggaagcga ccacttatcg aaacaaacgg
     3961 agaaacagga gaaatcgtgt gggacaaggg tagggatttc gcgacagtcc ggaaggtcct
     4021 gtccatgccg caggtgaaca tcgttaaaaa gaccgaagta cagaccggag gcttctccaa
     4081 ggaaagtatc ctcccgaaaa ggaacagcga caagctgatc gcacgcaaaa aagattggga
     4141 ccccaagaaa tacggcggat tcgattctcc tacagtcgct tacagtgtac tggttgtggc
     4201 caaagtggag aaagggaagt ctaaaaaact caaaagcgtc aaggaactgc tgggcatcac
     4261 aatcatggag cgatcaagct tcgaaaaaaa ccccatcgac tttctcgagg cgaaaggata
     4321 taaagaggtc aaaaaagacc tcatcattaa gcttcccaag tactctctct ttgagcttga
     4381 aaacggccgg aaacgaatgc tcgctagtgc gggcgagctg cagaaaggta acgagctggc
     4441 actgccctct aaatacgtta atttcttgta tctggccagc cactatgaaa agctcaaagg
     4501 gtctcccgaa gataatgagc agaagcagct gttcgtggaa caacacaaac actaccttga
     4561 tgagatcatc gagcaaataa gcgaattctc caaaagagtg atcctcgccg acgctaacct
     4621 cgataaggtg ctttctgctt acaataagca cagggataag cccatcaggg agcaggcaga
     4681 aaacattatc cacttgttta ctctgaccaa cttgggcgcg cctgcagcct tcaagtactt
     4741 cgacaccacc atagacagaa agcggtacac ctctacaaag gaggtcctgg acgccacact
     4801 gattcatcag tcaattacgg ggctctatga aacaagaatc gacctctctc agctcggtgg
     4861 agacggcggt acgtcttccg gtggctctgg tcccgctggt agctctggcg caacgagcgg
     4921 tggtaagtcc gctgaatact tgaacacctt cagattgaga aatttgggtt tgccagtcat
     4981 gaacaacttg cacgatatgt ctaaggctac cagaatttct gttgaaacct tgagattatt
     5041 gatctacacc gccgatttca gatacagaat ctacactgtt gaaaagaagg gtccagaaaa
     5101 gagaatgaga actatctacc aaccatccag agaattgaag gctttacaag gttgggtttt
     5161 gagaaacatc ttggacaagt tatcctcctc cccattttct atcggttttg aaaagcacca
     5221 atccatcttg aacaatgcca ctccacatat tggtgccaac ttcattttga acatcgactt
     5281 ggaagatttc ttcccatctt tgactgctaa caaggttttc ggtgttttcc attctttggg
     5341 ttacaacaga ttgatctcct ctgttttgac caagatttgc tgctacaaga acttgttgcc
     5401 acaaggtgct ccatcttctc caaaattggc taacttgatt tgctccaagt tggactacag
     5461 aattcaaggt tatgctggtt ctagaggttt gatatacact agatacgctg atgacttgac
     5521 cttgtctgct caatctatga agaaagttgt taaggccaga gacttcttgt tctccattat
     5581 tccatcagaa ggtttggtca tcaactccaa aaagacctgt atttctggtc caagatccca
     5641 aagaaaagtt accggtttgg ttatctccca agaaaaggtt ggtattggta gagaaaagta
     5701 caaagaaatc agagccaaga tccaccatat tttctgcggt aaatcctccg aaatcgaaca
     5761 tgttagaggt tggttgtctt tcatcttgtc cgttgattct aagtcccaca gaagattgat
     5821 tacctacatc tctaagttgg aaaagaagta cggtaagaac ccattgaaca aggctaagac
     5881 tccaccaaag aagaaaagaa aggtctgatc tcttctcgag tcatgtaatt agttatgtca
     5941 cgcttacatt cacgccctcc ccccacatcc gctctaaccg aaaaggaagg agttagacaa
     6001 cctgaagtct aggtccctat ttattttttt atagttatgt tagtattaag aacgttattt
     6061 atatttcaaa tttttctttt ttttctgtac agacgcgtgt acgcatgtaa cattatactg
     6121 aaaaccttgc ttgagaaggt tttgggacgc tcgaaggctt taatttcacc gcggtggagc
     6181 tccagctttt gttcccttta gtgagggtta attgcgcgct tggcgtaatc atggtcatag
     6241 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
     6301 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     6361 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
     6421 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     6481 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     6541 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     6601 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     6661 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     6721 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     6781 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     6841 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     6901 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     6961 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     7021 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     7081 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     7141 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     7201 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     7261 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     7321 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     7381 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     7441 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     7501 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     7561 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     7621 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     7681 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     7741 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     7801 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     7861 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     7921 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     7981 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     8041 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     8101 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     8161 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     8221 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     8281 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     8341 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc
     8401 attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg
     8461 cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct
     8521 tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc
     8581 gggtgtcggg gctggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat
     8641 aaattcccgt tttaagagct tggtgagcgc taggagtcac tgccaggtat cgtttgaaca
     8701 cggcattagt cagggaagtc ataacacagt cctttcccgc aattttcttt ttctattact
     8761 cttggcctcc tctagtacac tctatatttt tttatgcctc ggtaatgatt ttcatttttt
     8821 tttttccacc tagcggatga ctcttttttt ttcttagcga ttggcattat cacataatga
     8881 attatacatt atataaagta atgtgatttc ttcgaagaat atactaaaaa atgagcaggc
     8941 aagataaacg aaggcaaaga tgacagagca gaaagcccta gtaaagcgta ttacaaatga
     9001 aaccaagatt cagattgcga tctctttaaa gggtggtccc ctagcgatag agcactcgat
     9061 cttcccagaa aaagaggcag aagcagtagc agaacaggcc acacaatcgc aagtgattaa
     9121 cgtccacaca ggtatagggt ttctggacca tatgatacat gctctggcca agcattccgg
     9181 ctggtcgcta atcgttgagt gcattggtga cttacacata gacgaccatc acaccactga
     9241 agactgcggg attgctctcg gtcaagcttt taaagaggcc ctaggggccg tgcgtggagt
     9301 aaaaaggttt ggatcaggat ttgcgccttt ggatgaggca ctttccagag cggtggtaga
     9361 tctttcgaac aggccgtacg cagttgtcga acttggtttg caaagggaga aagtaggaga
     9421 tctctcttgc gagatgatcc cgcattttct tgaaagcttt gcagaggcta gcagaattac
     9481 cctccacgtt gattgtctgc gaggcaagaa tgatcatcac cgtagtgaga gtgcgttcaa
     9541 ggctcttgcg gttgccataa gagaagccac ctcgcccaat ggtaccaacg atgttccctc
     9601 caccaaaggt gttcttatgt agtgacaccg attatttaaa gctgcagcat acgatatata
     9661 tacatgtgta tatatgtata cctatgaatg tcagtaagta tgtatacgaa cagtatgata
     9721 ctgaagatga caaggtaatg catcattcta tacgtgtcat tctgaacgag gcgcgctttc
     9781 cttttttctt tttgcttttt cttttttttt ctcttgaact cgacggatct atgcggtgtg
     9841 aaataccgca cagatgcgta aggagaaaat accgcatcag gaaattgtaa gcgttaatat
     9901 tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga
     9961 aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc
    10021 agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac
    10081 cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc
    10141 gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg
    10201 gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag
    10261 ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc
    10321 gccgctacag ggcgcgtcca ttcgccattc aggctgcgca actgttggga agggcgatcg
    10381 gtgcgggcct cttcgctatt acgccag
//
