LOCUS       sequence-376684-        1413 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  R221C His SUMO SPOP expression plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1413)
  AUTHORS   Thomasen FE, Cuneo MJ, Mittag T, Lindorff-Larsen K
  TITLE     Conformational and oligomeric states of SPOP from small-angle X-ray 
            scattering and molecular dynamics simulations.
  JOURNAL   Elife. 2023 Mar 1;12:e84147. doi: 10.7554/eLife.84147.
  PUBMED    36856266
REFERENCE   2  (bases 1 to 1413)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2023-03-1"; volume: "12:e84147. doi"; pages: " 
            10.7554/eLife.84147"
COMMENT     Sequence Label: His SUMO TEV R221C SPOP 28-359
FEATURES             Location/Qualifiers
     source          1..1413
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..30
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             40..57
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             64..357
                     /codon_start=1
                     /gene="S. cerevisiae SMT3 (truncated)"
                     /product="cleavable ubiquitin-like protein tag"
                     /label=SUMO
                     /translation="MSDSEVNQEAKPEVKPEVKPGTHINLKVSDGSSEIFFKIKKTTPL
                     RRLMEAFAKRQGKEMDSLRFLYDGIRIQADQTPEDLDMEDNDIIEAHREQIGG"
     protein_bind    364..388
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             391..411
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 atgggcagca gccatcatca tcatcatcac ggcagcggcc tggtgccgcg cggcagcgct
       61 agcatgtcgg actcagaagt caatcaagaa gctaagccag aggtcaagcc agaagtcaag
      121 cctgggactc acatcaattt aaaggtgtcc gatggatctt cagagatctt cttcaagatc
      181 aaaaagacca ctcctttaag aaggctgatg gaagcgttcg ctaaaagaca gggtaaggaa
      241 atggactcct taagattctt gtacgacggt attagaattc aagctgatca gacccctgaa
      301 gatttggaca tggaggataa cgatattatt gaggctcaca gagaacagat tggtggtagt
      361 atcacaagtt tgtacaaaaa agcaggcttc gaaaacctgt attttcaggg cagcaaggta
      421 gtgaaattct cctacatgtg gaccatcaat aactttagct tttgccggga ggaaatgggt
      481 gaagtcatta aaagttctac attttcatca ggagcaaatg ataaactgaa atggtgtttg
      541 cgagtaaacc ccaaagggtt agatgaagaa agcaaagatt acctgtcact ttacctgtta
      601 ctggtcagct gtccaaagag tgaagttcgg gcaaaattca aattctccat cctgaatgcc
      661 aagggagaag aaaccaaagc tatggagagt caacgggcat ataggtttgt gcaaggcaaa
      721 gactggggat tcaagaaatt catccgtaga gattttcttt tggatgaggc caacgggctt
      781 ctccccgatg acaagcttac cctcttctgc gaggtgagtg ttgtgcaaga ttctgtcaac
      841 atttctggcc agaataccat gaacatggta aaggttcctg agtgccggct ggcagatgag
      901 ttaggaggac tgtgggagaa ttcccggttc acagactgct gcttgtgtgt tgccggccag
      961 gaattccagg ctcacaaggc tatcttagca gcttgttctc cggtttttag tgccatgttt
     1021 gaacatgaaa tggaggagag caaaaagaat cgagttgaaa tcaatgatgt ggagcctgaa
     1081 gtttttaagg aaatgatgtg cttcatttac acggggaagg ctccaaacct cgacaaaatg
     1141 gctgatgatt tgctggcagc tgctgacaag tatgccctgg agcgcttaaa ggtcatgtgt
     1201 gaggatgccc tctgcagtaa cctgtccgtg gagaacgctg cagaaattct catcctggcc
     1261 gacctccaca gtgcagatca gttgaaaact caggcagtgg atttcatcaa ctatcatgct
     1321 tcggatgtct tggagacctc tgggtggaag tcaatggtgg tgtcacatcc ccacttggtg
     1381 gctgaggcat accgctctct ggcttcagca taa
//
