LOCUS       sequence-37669-e         799 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 799)
  AUTHORS   Mukherji S, Ebert MS, Zheng GX, Tsang JS, Sharp PA, van Oudenaarden 
            A
  TITLE     MicroRNAs can generate thresholds in target gene expression.
  JOURNAL   Nat Genet. 2011 Aug 21;43(9):854-9. doi: 10.1038/ng.905.
  PUBMED    21857679
REFERENCE   2  (bases 1 to 799)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ng"; 
            journalName: "Nat Genet"; date: "2011-08-21- 21"; volume: "43"; 
            issue: "9"; pages: "854-9"
COMMENT     Sequence Label: pTRETightBI-RY-1
FEATURES             Location/Qualifiers
     source          1..799
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    431..512
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(468..487)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        545..649
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gggctcctcg ancggagtcc ccgaggangg cgccctgaag ggcgagatca agcagaggct
       61 gaagctgaag gacggcggcc actacgacgc tgaggtcaag accacctaca aggccaagaa
      121 gcccgtgcag ctgcccggcg cctacaacgt caacatcaag ttggacatca cctcccacaa
      181 cgaggactac accatcgtgg aacagtacga acgcgccgag ggccgccact ccaccggcgg
      241 catggacgag ctgtacaagt aactcgagcc ggtacctgca ctcgcgcact ttaatcgata
      301 agcttgtcga cgatatctcc agaggatcat aatcagccat accacatttg tagaggtttt
      361 acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat
      421 tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
      481 aaatttcaca aataaagcat ttttttcact gccgacgtca ggtggcactt ttcggggaaa
      541 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
      601 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
      661 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
      721 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
      781 catcgaactg gatctcaac
//
