LOCUS       sequence-37671-e         873 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 873)
  AUTHORS   Kajimoto T, Sawamura S, Tohyama Y, Mori Y, Newton AC
  TITLE     Protein kinase C {delta}-specific activity reporter reveals 
            agonist-evoked nuclear activity controlled by Src family of kinases.
  JOURNAL   J Biol Chem. 2010 Dec 31. 285(53):41896-910.
  PUBMED    20959447
REFERENCE   2  (bases 1 to 873)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2010 Dec 31. 285(53):41896-910."
COMMENT     Sequence Label: pcDNA3-deltaCKAR
FEATURES             Location/Qualifiers
     source          1..873
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(35..56)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             complement(join(191..703,704..706))
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIE"
     primer_bind     384..403
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     643..664
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 gctcgtccat gccgtgagtg atcccggcgg cggtcacgaa ctccagcagg accatgtgat
       61 cgcgcttctc gttggggtct ttgctcagct tggactggta gctcaggtag tggttgtcgg
      121 gcagcagcac ggggccgtcg ccgatggggg tgttctgctg gtagtggtcg gcgagctgca
      181 cgctgccgtc ctcgatgttg tggcggatct tgaagttcac cttgatgccg ttcttctgct
      241 tgtcggccat gatatagacg ttgtggctgt tgtagttgta ctccagcttg tgccccagga
      301 tgttgccgtc ctccttgaag tcgatgccct tcagctcgat gcggttcacc agggtgtcgc
      361 cctcgaactt cacctcggcg cgggtcttgt agttgccgtc gtccttgaag aagatggtgc
      421 gctcctggac gtagccttcg ggcatggcgg acttgaagaa gtcgtgctgc ttcatgtggt
      481 cggggtagcg ggcgaagcac atcaggccgt agccgaaggt ggtcacgagg gtgggccagg
      541 gcacgggcag cttgccggtg gtgcagatga acttcagggt cagcttgccg taggtggcat
      601 cgccctcgcc ctcgccggac acgctgaact tgtggccgtt tacgtcgccg tccagctcga
      661 ccaggatggg caccaccccg gtgaacagct cctcgccctt gctcacgaga attccaccag
      721 atccaccccg cacctgtcgg agctgcccaa tagcgaaggt gctacggctg gacacacccc
      781 gacctccgga tccccctaac tttttcacca aatctttttc ttcggctgtt tgcttaagta
      841 ctactctttg ttcttgtaac atacctaatc cct
//
