LOCUS       sequence-37672-e         760 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 760)
  AUTHORS   Mukherji S, Ebert MS, Zheng GX, Tsang JS, Sharp PA, van Oudenaarden 
            A
  TITLE     MicroRNAs can generate thresholds in target gene expression.
  JOURNAL   Nat Genet. 2011 Aug 21;43(9):854-9. doi: 10.1038/ng.905.
  PUBMED    21857679
REFERENCE   2  (bases 1 to 760)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ng"; 
            journalName: "Nat Genet"; date: "2011-08-21- 21"; volume: "43"; 
            issue: "9"; pages: "854-9"
COMMENT     Sequence Label: pTRETightBI-RY-4
FEATURES             Location/Qualifiers
     source          1..760
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    498..579
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(535..554)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        612..716
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cggagtcccc gaggaggcgc cctgaagggc gagatcaagc agaggctgaa gctgaaggac
       61 ggcggccact acgacgctga ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg
      121 cccggcgcct acaacgtcaa catcaagttg gacatcacct cccacaacga ggactacacc
      181 atcgtggaac agtacgaacg cgccgagggc cgccactcca ccggcggcat ggacgagctg
      241 tacaagtaaa tcgatccgcc tcgagccggt acctgcactc gcgcacttta ccggtacctg
      301 cactcgcgca ctttaccggt acctgcactc gcgcacttta ccggtacctg cactcgcgca
      361 ctttaccgga tcgcgggcga tatctccaga ggatcataat cagccatacc acatttgtag
      421 aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga
      481 atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata
      541 gcatcacaaa tttcacaaat aaagcatttt tttcactgcc gacgtcaggt ggcacttttc
      601 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
      661 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
      721 gtattcaaca tttccgtgtc gcccttattc ccttttttgc
//
