LOCUS       sequence-376720-        8620 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For use in RBP tethering screen.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8620)
  AUTHORS   Luo EC, Nathanson JL, Tan FE, Schwartz JL, Schmok JC, Shankar A, 
            Markmiller S, Yee BA, Sathe S, Pratt GA, Scaletta DB, Ha Y, Hill DE,
            Aigner S, Yeo GW
  TITLE     Large-scale tethered function assays identify factors that regulate 
            mRNA stability and translation.
  JOURNAL   Nat Struct Mol Biol. 2020 Oct;27(10):989-1000. doi: 
            10.1038/s41594-020-0477-6. Epub 2020 Aug 17.
  PUBMED    32807991
REFERENCE   2  (bases 1 to 8620)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41594-020-0477-6"; journalName: "Nat Struct Mol Biol"; 
            date: "2020-10"; volume: "27"; issue: "10"; pages: "989-1000"
COMMENT     Sequence Label: CNOT3
FEATURES             Location/Qualifiers
     source          1..8620
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     primer_bind     1196..1215
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1196..1214
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1293..1317
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3577..3601)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3726..3767
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(4222..4239)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4228..4452
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(4585..4604)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    4736..4783
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             4791..5813
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             4791..5813
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    5943..6064
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5980..5999)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6034..6053
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6113..6129)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6113..6129)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6126..6148)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6137..6153
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6161..6167,6168..6185,6186..6191))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6206..6227
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6344..6361)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6515..7103)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6595..6614)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7274..8065,8066..8134))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7897..7916
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8135..8239)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8314..8333)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gaattagctt ggtactaata
     1201 cgactcacta tagggagacc caagctggct aggtaagctt ggtaccgagc tcggatccac
     1261 tagtccagtg tggtggaatt ctgcagatat caacaagttt gtacaaaaaa gttggcatgg
     1321 cggacaagcg caaactccaa ggtgagattg atcgctgcct caagaaggtg tccgagggcg
     1381 tggagcagtt tgaagatatt tggcagaagc tccacaatgc agccaacgcg aaccagaaag
     1441 aaaagtatga ggctgaccta aagaaggaga ttaagaagct acaacggctg agggaccaaa
     1501 tcaagacatg ggtagcgtcc aacgagatca aggacaagag gcagcttata gacaaccgca
     1561 agctcattga gacgcaaatg gaacggttca aagttgtgga acgagagacc aaaaccaaag
     1621 cttacagcaa agagggcctg ggcctggccc agaaggtaga tcctgcccag aaggagaagg
     1681 aagaggttgg ccagtggctc acgaatacca tcgacacgct caacatgcag gtggaccagt
     1741 ttgagagtga agtggagtca ctgtcagtgc agacacgcaa gaagaagggc gacaaggata
     1801 agcaggaccg gattgagggc ttgaagcggc acatcgagaa gcaccgctac cacgtgcgca
     1861 tgctagagac catcctgcgc atgctggaca atgactccat cctcgttgac gccatccgca
     1921 agatcaagga cgacgttgag tactatgttg actcatccca ggaccccgac ttcgaggaga
     1981 acgagtttct ctacgatgac ctggacctcg aggacattcc acaggcgctg gtcgccacct
     2041 ccccccccag ccacagccac atggaggatg agatcttcaa ccagtccagc agcacgccca
     2101 cctcaaccac ctccagctct cccatcccgc ccagcccagc caactgtacc acggaaaact
     2161 ctgaagatga taagaagagg ggacgttcca cagacagtga agtcagccag tctccagcca
     2221 aaaacggctc caagcctgtc cacagcaacc agcaccctca gtccccagct gtgccgccca
     2281 cctacccctc cggccccccg cctgctgcct ctgccttgag caccactcct ggcaacaatg
     2341 gggtccccgc ccccgcagca cccccaagtg ccctgggccc caaggccagt ccagctccca
     2401 gccacaactc gggcacccct gctccctatg cccaggcggt ggccccacca gctcccagtg
     2461 ggcccagcac gacccagccc cggcccccca gcgtccagcc tagcggaggc ggaggcggcg
     2521 gcagcggagg cggagggagc agcagcagta gtaacagcag tgccggtgga ggggctggca
     2581 agcagaatgg cgccaccagt tacagctcag ttgtggcaga cagcccggca gaggtggctt
     2641 tgagcagcag tgggggcaac aatgccagca gccaggcctt gggcccccct tccggccccc
     2701 acaacccacc tcccagcacc tcgaaggaac ccagtgcggc agccccaacg ggggctgggg
     2761 gcgtggcccc aggctcaggg aacaactcag ggggacccag cctcctggtg ccactgcctg
     2821 tgaatcctcc cagctcccca acgcccagct tcagtgatgc caaggcagcc ggtgccctgc
     2881 tcaatgggcc tccacagttc agcaccgccc cagaaatcaa ggcccctgag cctctgagct
     2941 ccttgaagtc catggcggaa cgggcagcca tcagctctgg cattgaggac cctgtgccaa
     3001 cgctgcacct gaccgagcga gacatcatcc tgagcagtac atcagcacct ccggcctcag
     3061 cccagccgcc cctgcagctg tcagaggtga acataccgct gtcgctgggt gtctgtccac
     3121 tgggccctgt gcccctcacc aaggagcagc tctatcagca ggccatggaa gaggccgcct
     3181 ggcaccacat gcctcacccc tctgactctg agcgtattcg gcagtacctc ccccggaacc
     3241 cctgtccgac gcccccctac caccaccaga tgccaccccc acactcggac actgtggaat
     3301 tctaccagcg cctgtcgacc gagacactct tcttcatctt ctactatctg gagggcacta
     3361 aggcacagta tctggcagcc aaggccctaa agaagcagtc atggcgattc cacaccaagt
     3421 acatgatgtg gttccagagg cacgaggagc ccaagaccat cactgacgag tttgagcagg
     3481 gcacctacat ctactttgac tacgagaagt ggggccagcg gaagaaggaa ggcttcacct
     3541 ttgagtaccg ctacctggag gaccgggacc tccagtgccc aactttcttg tacaaagtgg
     3601 ttgatatcca gcacagtggc ggccgctcga gtctagaggg cccgcggttc gaaaccagcg
     3661 gaggcggcgg aagcggggga ggaggcagcg gcggaggcgg cagcgggggc ggcggaagcg
     3721 ctaccggtaa gcctatccct aaccctctcc tcggtctcga ttctacgcgt accggtatgg
     3781 cttctaactt tactcagttc gttctcgtcg acaatggcgg aactggcgac gtgactgtcg
     3841 ccccaagcaa cttcgctaac ggggtcgctg aatggatcag ctctaactcg cgatcacagg
     3901 cttacaaagt aacctgtagc gttcgtcaga gctctgcgca gaatcgcaaa tacaccatca
     3961 aagtcgaggt gcctaaagtg gcaacccaga ctgttggtgg tgaagagctt cctgtagccg
     4021 gatggagatc ttacttaaat atggaactaa ccattccaat tttcgccacg aattccgact
     4081 gcgagcttat tgttaaggca atgcaaggtc tcctaaaaga tggaaacccg attccctcgg
     4141 ccatcgcggc caactccggc atctacggag gtggaggtgg atctcgtacg ggttagtaat
     4201 gagtttaaac ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt
     4261 gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat
     4321 aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg
     4381 tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg
     4441 tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg
     4501 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
     4561 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
     4621 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
     4681 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt acctagaagt
     4741 tcctattccg aagttcctat tctctagaaa gtataggaac ttccttggcc aaaaagcctg
     4801 aactcaccgc gacgtctgtc gagaagtttc tgatcgaaaa gttcgacagc gtctccgacc
     4861 tgatgcagct ctcggagggc gaagaatctc gtgctttcag cttcgatgta ggagggcgtg
     4921 gatatgtcct gcgggtaaat agctgcgccg atggtttcta caaagatcgt tatgtttatc
     4981 ggcactttgc atcggccgcg ctcccgattc cggaagtgct tgacattggg gaattcagcg
     5041 agagcctgac ctattgcatc tcccgccgtg cacagggtgt cacgttgcaa gacctgcctg
     5101 aaaccgaact gcccgctgtt ctgcagccgg tcgcggaggc catggatgcg atcgctgcgg
     5161 ccgatcttag ccagacgagc gggttcggcc cattcggacc gcaaggaatc ggtcaataca
     5221 ctacatggcg tgatttcata tgcgcgattg ctgatcccca tgtgtatcac tggcaaactg
     5281 tgatggacga caccgtcagt gcgtccgtcg cgcaggctct cgatgagctg atgctttggg
     5341 ccgaggactg ccccgaagtc cggcacctcg tgcacgcgga tttcggctcc aacaatgtcc
     5401 tgacggacaa tggccgcata acagcggtca ttgactggag cgaggcgatg ttcggggatt
     5461 cccaatacga ggtcgccaac atcttcttct ggaggccgtg gttggcttgt atggagcagc
     5521 agacgcgcta cttcgagcgg aggcatccgg agcttgcagg atcgccgcgg ctccgggcgt
     5581 atatgctccg cattggtctt gaccaactct atcagagctt ggttgacggc aatttcgatg
     5641 atgcagcttg ggcgcagggt cgatgcgacg caatcgtccg atccggagcc gggactgtcg
     5701 ggcgtacaca aatcgcccgc agaagcgcgg ccgtctggac cgatggctgt gtagaagtac
     5761 tcgccgatag tggaaaccga cgccccagca ctcgtccgag ggcaaaggaa tagcacgtac
     5821 tacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc
     5881 gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc
     5941 ccaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     6001 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     6061 cttatcatgt ctgtataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc
     6121 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     6181 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
     6241 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
     6301 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
     6361 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     6421 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     6481 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     6541 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     6601 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     6661 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     6721 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     6781 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     6841 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     6901 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     6961 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     7021 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     7081 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     7141 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     7201 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     7261 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     7321 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     7381 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     7441 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     7501 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     7561 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     7621 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     7681 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     7741 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     7801 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     7861 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     7921 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     7981 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     8041 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     8101 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     8161 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     8221 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc gacggatcgg
     8281 gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
     8341 aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat
     8401 ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc ttagggttag
     8461 gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt gattattgac
     8521 taggcttttg caaaaagctt tgcaaagatg gataaagttt taaacagaga ggaatctttg
     8581 cagctaatgg accttctagg tcttgaaagg agtgggaatt
//
