LOCUS       sequence-376726-        9082 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For use in RBP tethering screen.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9082)
  AUTHORS   Luo EC, Nathanson JL, Tan FE, Schwartz JL, Schmok JC, Shankar A, 
            Markmiller S, Yee BA, Sathe S, Pratt GA, Scaletta DB, Ha Y, Hill DE,
            Aigner S, Yeo GW
  TITLE     Large-scale tethered function assays identify factors that regulate 
            mRNA stability and translation.
  JOURNAL   Nat Struct Mol Biol. 2020 Oct;27(10):989-1000. doi: 
            10.1038/s41594-020-0477-6. Epub 2020 Aug 17.
  PUBMED    32807991
REFERENCE   2  (bases 1 to 9082)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41594-020-0477-6"; journalName: "Nat Struct Mol Biol"; 
            date: "2020-10"; volume: "27"; issue: "10"; pages: "989-1000"
COMMENT     Sequence Label: DDX11-1
FEATURES             Location/Qualifiers
     source          1..9082
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        96..1274
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          327..1265
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1222..1242
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     primer_bind     1291..1310
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1291..1309
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1388..1412
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    2063..2074
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
     protein_bind    complement(4134..4158)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4283..4324
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(4779..4796)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4785..5009
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5142..5161)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    5293..5340
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             5348..6370
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             5348..6370
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    6500..6621
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6537..6556)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6591..6610
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6670..6686)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6670..6686)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6683..6705)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6694..6710
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6718..6724,6725..6742,6743..6748))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6763..6784
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6901..6918)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7072..7660)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7152..7171)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7831..8622,8623..8691))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8454..8473
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8692..8796)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8871..8890)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 ttttgcaaaa agctttgcaa agatggataa agttttaaac agagaggaat ctttgcagct
       61 aatggacctt ctaggtcttg aaaggagtgg gaattggctc cggtgcccgt cagtgggcag
      121 agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaaccggtg
      181 cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt
      241 ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc
      301 gcaacgggtt tgccgccaga acacaggtaa gtgccgtgtg tggttcccgc gggcctggcc
      361 tctttacggg ttatggccct tgcgtgcctt gaattacttc cacctggctg cagtacgtga
      421 ttcttgatcc cgagcttcgg gttggaagtg ggtgggagag ttcgaggcct tgcgcttaag
      481 gagccccttc gcctcgtgct tgagttgagg cctggcctgg gcgctggggc cgccgcgtgc
      541 gaatctggtg gcaccttcgc gcctgtctcg ctgctttcga taagtctcta gccatttaaa
      601 atttttgatg acctgctgcg acgctttttt tctggcaaga tagtcttgta aatgcgggcc
      661 aagatctgca cactggtatt tcggtttttg gggccgcggg cggcgacggg gcccgtgcgt
      721 cccagcgcac atgttcggcg aggcggggcc tgcgagcgcg gccaccgaga atcggacggg
      781 ggtagtctca agctggccgg cctgctctgg tgcctggcct cgcgccgccg tgtatcgccc
      841 cgccctgggc ggcaaggctg gcccggtcgg caccagttgc gtgagcggaa agatggccgc
      901 ttcccggccc tgctgcaggg agctcaaaat ggaggacgcg gcgctcggga gagcgggcgg
      961 gtgagtcacc cacacaaagg aaaagggcct ttccgtcctc agccgtcgct tcatgtgact
     1021 ccacggagta ccgggcgccg tccaggcacc tcgattagtt ctcgagcttt tggagtacgt
     1081 cgtctttagg ttggggggag gggttttatg cgatggagtt tccccacact gagtgggtgg
     1141 agactgaagt taggccagct tggcacttga tgtaattctc cttggaattt gccctttttg
     1201 agtttggatc ttggttcatt ctcaagcctc agacagtggt tcaaagtttt tttcttccat
     1261 ttcaggtgtc gtgaggaatt agcttggtac taatacgact cactataggg agacccaagc
     1321 tggctaggta agcttggtac cgagctcgga tccactagtc cagtgtggtg gaattctgca
     1381 gatatcaaca agtttgtaca aaaaagttgg caccatggct aatgaaacac agaaggttgg
     1441 tgccatccat tttccttttc ccttcacacc ctattccatc caggaagact tcatggcaga
     1501 gctgtaccgg gttttggagg ctggcaagat tgggatattt gagagtccaa ctggcactgg
     1561 gaagtcctta agtcttattt gtggggccct ctcttggctc cgtgactttg aacagaagaa
     1621 gcgtgaagaa gaggcacgac tccttgaaac tggaactggc cccttacatg atgagaaaga
     1681 tgaatccctg tgtctgtctt cttcctgcga aggggctgca ggcaccccga ggcctgctgg
     1741 agaaccggcc tgggttactc agtttgtgca gaagaaagaa gagagggacc tggtggaccg
     1801 actaaaggcg gagcaggcca ggaggaagca gcgagaagaa cgcctgcagc agctgcagca
     1861 cagggtgcag ctcaagtatg cagccaagcg cctgaggcag gaagaagaag aaagagagaa
     1921 tctcctccgc ctcagcaggg agatgctaga gacaggcccg gaggctgagc ggctggagca
     1981 gctggagtct ggggaggagg agctggtcct cgccgaatac gagagtgatg aggagaaaaa
     2041 ggtggcgagc agagtggatg aggatgagga tgacctggag gaagaacaca taactaagat
     2101 ttattactgt agtcggacac actcccagct ggcccagttt gtgcatgagg tgaagaagag
     2161 cccctttggc aaggatgttc ggctggtctc ccttggctcc cggcagaacc tttgtgtaaa
     2221 tgaagacgtg aaaagcctag gttctgtgca gcttatcaac gaccgctgtg tggacatgca
     2281 gagaagcagg cacgagaaga agaaaggagc tgaggaggag aagccaaaga ggaggaggca
     2341 ggagaagcag gcagcctgcc ccttctacaa ccacgagcag atgggccttc tccgggatga
     2401 ggccctggca gaggtgaagg acatggagca gctgctggcc cttgggaagg aggcccgggc
     2461 ctgtccctat tacgggagcc gccttgccat ccctgcagcc cagctggtgg tgctgcccta
     2521 tcagatgctg ctgcatgcgg ccactcggca ggccgcgggc atccggctgc aggaccaggt
     2581 ggtgatcatc gacgaggcgc acaacctgat cgacaccatc acgggcatgc acagcgtgga
     2641 ggtcagcggc tcccagctct gccaggccca ttcccagctg ctgcagtacg tggagcgata
     2701 cgggaagcgt ttgaaggcca agaacctgat gtacctgaag cagatcctgt atttgctgga
     2761 gaaattcgtg gctgtgctag gggggaacat taagcaaaat cccaatacac agagtctgtc
     2821 acagacaggg acggagctga agaccatcaa cgactttctc ttccagagcc agatcgacaa
     2881 catcaacctg ttcaaggtgc agcgatactg tgagaagagc atgatcagca gaaagctctt
     2941 tggcttcact gaacggtacg gagcagtgtt ctcatcccgg gagcagccca aactggctgg
     3001 gtttcagcaa ttcctgcaga gcctgcagcc caggacgact gaagcacttg cagcccctgc
     3061 agacgagagt caggccagcg tcccgcaacc agcttcccca ctgatgcaca tcgaaggctt
     3121 cctggcagct ctcactacgg ccaaccagga cggcagggtc atcctgagcc gccaaggcag
     3181 tctcagtcag agcaccctga agtttttgct cctgaatcca gctgtgcact ttgcccaagt
     3241 ggtgaaggaa tgccgggcag tggtcattgc cggggggacc atgcagccgg tgtctgactt
     3301 ccggcagcag ctgctggcct gtgccggggt ggaagctgag cgcgtggtgg agttttcctg
     3361 tggtcacgtg atccctccag acaacatcct gccccttgtc atctgcatcg gggtctccaa
     3421 ccagccgcta gaattcacgt tccagaaaag agacctgcct cagatgatgg acgaggtggg
     3481 tcgcatcctc tgtaacctgt gcagtgtggt ttctggaggg gtggtctgtt tcttcccctc
     3541 ctacgagtac ctgcgccagg tccatgccca ctgggagaag ggtggcctgc tgggccatct
     3601 ggctgccagg aagaagatat tccaggaacc taagagcgca caccaggtag agcaggtgct
     3661 gctggcgtat tccaggtgca tccaggcctg tggccaggag agaggccccg tgacaggggc
     3721 cctgctcctc tctgtggttg gaggaaagat gagtgaaggg atcaacttct ctgacaacct
     3781 aggccggtgt gtggtgatgg tgggcatgcc cttccccaac atcaggtctg cagagctgca
     3841 ggagaagatg gcctacttgg atcaaaccct ccccagagcc cccggccagg cacccccagg
     3901 gaaggctctg gtggagaacc tgtgcatgaa ggccgtcaac cagtccatag gcagggccat
     3961 caggcaccag aaggattttg ccagcatagt gctcctggac cagcgatacg cccggccccc
     4021 tgtcctggcc aagctgccgg cctggatccg agcccgcgtg gaggtcaaag ctacctttgg
     4081 ccccgccatt gctgctgtgc agaagtttca ccgggagaag tcggcctctt ccttgccaac
     4141 tttcttgtac aaagtggttg atatccagca cagtggcggc cgctcgagtc tagagggccc
     4201 gcggttcgaa accagcggag gcggcggaag cgggggagga ggcagcggcg gaggcggcag
     4261 cgggggcggc ggaagcgcta ccggtaagcc tatccctaac cctctcctcg gtctcgattc
     4321 tacgcgtacc ggtatggctt ctaactttac tcagttcgtt ctcgtcgaca atggcggaac
     4381 tggcgacgtg actgtcgccc caagcaactt cgctaacggg gtcgctgaat ggatcagctc
     4441 taactcgcga tcacaggctt acaaagtaac ctgtagcgtt cgtcagagct ctgcgcagaa
     4501 tcgcaaatac accatcaaag tcgaggtgcc taaagtggca acccagactg ttggtggtga
     4561 agagcttcct gtagccggat ggagatctta cttaaatatg gaactaacca ttccaatttt
     4621 cgccacgaat tccgactgcg agcttattgt taaggcaatg caaggtctcc taaaagatgg
     4681 aaacccgatt ccctcggcca tcgcggccaa ctccggcatc tacggaggtg gaggtggatc
     4741 tcgtacgggt tagtaatgag tttaaacccg ctgatcagcc tcgactgtgc cttctagttg
     4801 ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc
     4861 cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc
     4921 tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag
     4981 gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc
     5041 tagggggtat ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
     5101 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
     5161 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
     5221 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg
     5281 ttcacgtacc tagaagttcc tattccgaag ttcctattct ctagaaagta taggaacttc
     5341 cttggccaaa aagcctgaac tcaccgcgac gtctgtcgag aagtttctga tcgaaaagtt
     5401 cgacagcgtc tccgacctga tgcagctctc ggagggcgaa gaatctcgtg ctttcagctt
     5461 cgatgtagga gggcgtggat atgtcctgcg ggtaaatagc tgcgccgatg gtttctacaa
     5521 agatcgttat gtttatcggc actttgcatc ggccgcgctc ccgattccgg aagtgcttga
     5581 cattggggaa ttcagcgaga gcctgaccta ttgcatctcc cgccgtgcac agggtgtcac
     5641 gttgcaagac ctgcctgaaa ccgaactgcc cgctgttctg cagccggtcg cggaggccat
     5701 ggatgcgatc gctgcggccg atcttagcca gacgagcggg ttcggcccat tcggaccgca
     5761 aggaatcggt caatacacta catggcgtga tttcatatgc gcgattgctg atccccatgt
     5821 gtatcactgg caaactgtga tggacgacac cgtcagtgcg tccgtcgcgc aggctctcga
     5881 tgagctgatg ctttgggccg aggactgccc cgaagtccgg cacctcgtgc acgcggattt
     5941 cggctccaac aatgtcctga cggacaatgg ccgcataaca gcggtcattg actggagcga
     6001 ggcgatgttc ggggattccc aatacgaggt cgccaacatc ttcttctgga ggccgtggtt
     6061 ggcttgtatg gagcagcaga cgcgctactt cgagcggagg catccggagc ttgcaggatc
     6121 gccgcggctc cgggcgtata tgctccgcat tggtcttgac caactctatc agagcttggt
     6181 tgacggcaat ttcgatgatg cagcttgggc gcagggtcga tgcgacgcaa tcgtccgatc
     6241 cggagccggg actgtcgggc gtacacaaat cgcccgcaga agcgcggccg tctggaccga
     6301 tggctgtgta gaagtactcg ccgatagtgg aaaccgacgc cccagcactc gtccgagggc
     6361 aaaggaatag cacgtactac gagatttcga ttccaccgcc gccttctatg aaaggttggg
     6421 cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct
     6481 ggagttcttc gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa
     6541 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     6601 caaactcatc aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc
     6661 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     6721 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     6781 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
     6841 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     6901 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     6961 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     7021 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     7081 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     7141 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     7201 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     7261 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     7321 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     7381 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     7441 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     7501 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     7561 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     7621 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     7681 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     7741 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     7801 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     7861 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     7921 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     7981 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     8041 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     8101 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     8161 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     8221 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     8281 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     8341 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     8401 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     8461 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     8521 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     8581 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     8641 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     8701 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     8761 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
     8821 tgacgtcgac ggatcgggag atctcccgat cccctatggt gcactctcag tacaatctgc
     8881 tctgatgccg catagttaag ccagtatctg ctccctgctt gtgtgttgga ggtcgctgag
     8941 tagtgcgcga gcaaaattta agctacaaca aggcaaggct tgaccgacaa ttgcatgaag
     9001 aatctgctta gggttaggcg ttttgcgctg cttcgcgatg tacgggccag atatacgcgt
     9061 tgacattgat tattgactag gc
//
