LOCUS       sequence-376728-        8710 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For use in RBP tethering screen.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8710)
  AUTHORS   Luo EC, Nathanson JL, Tan FE, Schwartz JL, Schmok JC, Shankar A, 
            Markmiller S, Yee BA, Sathe S, Pratt GA, Scaletta DB, Ha Y, Hill DE,
            Aigner S, Yeo GW
  TITLE     Large-scale tethered function assays identify factors that regulate 
            mRNA stability and translation.
  JOURNAL   Nat Struct Mol Biol. 2020 Oct;27(10):989-1000. doi: 
            10.1038/s41594-020-0477-6. Epub 2020 Aug 17.
  PUBMED    32807991
REFERENCE   2  (bases 1 to 8710)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41594-020-0477-6"; journalName: "Nat Struct Mol Biol"; 
            date: "2020-10"; volume: "27"; issue: "10"; pages: "989-1000"
COMMENT     Sequence Label: DDX21
FEATURES             Location/Qualifiers
     source          1..8710
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        136..1314
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          367..1305
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1262..1282
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     primer_bind     1331..1350
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1331..1349
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1428..1452
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3802..3826)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3951..3992
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(4447..4464)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4453..4677
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(4810..4829)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    4961..5008
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             5016..6038
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             5016..6038
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    6168..6289
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6205..6224)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6259..6278
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6338..6354)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6338..6354)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6351..6373)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6362..6378
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6386..6392,6393..6410,6411..6416))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6431..6452
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6569..6586)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6740..7328)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6820..6839)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7499..8290,8291..8359))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8122..8141
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8360..8464)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8539..8558)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 cgggccagat atacgcgttg acattgatta ttgactaggc ttttgcaaaa agctttgcaa
       61 agatggataa agttttaaac agagaggaat ctttgcagct aatggacctt ctaggtcttg
      121 aaaggagtgg gaattggctc cggtgcccgt cagtgggcag agcgcacatc gcccacagtc
      181 cccgagaagt tggggggagg ggtcggcaat tgaaccggtg cctagagaag gtggcgcggg
      241 gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa
      301 ccgtatataa gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt tgccgccaga
      361 acacaggtaa gtgccgtgtg tggttcccgc gggcctggcc tctttacggg ttatggccct
      421 tgcgtgcctt gaattacttc cacctggctg cagtacgtga ttcttgatcc cgagcttcgg
      481 gttggaagtg ggtgggagag ttcgaggcct tgcgcttaag gagccccttc gcctcgtgct
      541 tgagttgagg cctggcctgg gcgctggggc cgccgcgtgc gaatctggtg gcaccttcgc
      601 gcctgtctcg ctgctttcga taagtctcta gccatttaaa atttttgatg acctgctgcg
      661 acgctttttt tctggcaaga tagtcttgta aatgcgggcc aagatctgca cactggtatt
      721 tcggtttttg gggccgcggg cggcgacggg gcccgtgcgt cccagcgcac atgttcggcg
      781 aggcggggcc tgcgagcgcg gccaccgaga atcggacggg ggtagtctca agctggccgg
      841 cctgctctgg tgcctggcct cgcgccgccg tgtatcgccc cgccctgggc ggcaaggctg
      901 gcccggtcgg caccagttgc gtgagcggaa agatggccgc ttcccggccc tgctgcaggg
      961 agctcaaaat ggaggacgcg gcgctcggga gagcgggcgg gtgagtcacc cacacaaagg
     1021 aaaagggcct ttccgtcctc agccgtcgct tcatgtgact ccacggagta ccgggcgccg
     1081 tccaggcacc tcgattagtt ctcgagcttt tggagtacgt cgtctttagg ttggggggag
     1141 gggttttatg cgatggagtt tccccacact gagtgggtgg agactgaagt taggccagct
     1201 tggcacttga tgtaattctc cttggaattt gccctttttg agtttggatc ttggttcatt
     1261 ctcaagcctc agacagtggt tcaaagtttt tttcttccat ttcaggtgtc gtgaggaatt
     1321 agcttggtac taatacgact cactataggg agacccaagc tggctaggta agcttggtac
     1381 cgagctcgga tccactagtc cagtgtggtg gaattctgca gatatcaaca agtttgtaca
     1441 aaaaagttgg catgccggga aaactccgta gtgacgctgg tttggaatca gacaccgcaa
     1501 tgaaaaaagg ggagacactg cgaaagcaaa ccgaggagaa agagaaaaaa gagaagccaa
     1561 aatctgataa gactgaagag atagcagaag aggaagaaac tgttttcccc aaagctaaac
     1621 aagttaaaaa gaaagcagag ccttctgaag ttgacatgaa ttctcctaaa tccaaaaagg
     1681 caaaaaagaa agaggagcca tctcaaaatg acatttctcc taaaaccaaa agtttgagaa
     1741 agaaaaagga gcccattgaa aagaaagtgg tttcttctaa aaccaaaaaa gtgacaaaaa
     1801 atgaggagcc ttctgaggaa gaaatagatg ctcctaagcc caagaagatg aagaaagaaa
     1861 aggaaatgaa tggagaaact agagagaaaa gccccaaact gaagaatgga tttcctcatc
     1921 ctgaaccgga ctgtaacccc agtgaagctg ccagtgaaga aagtaacagt gagatagagc
     1981 aggaaatacc tgtggaacaa aaagaaggcg ctttctctaa ttttcccata tctgaagaaa
     2041 ctattaaact tctcaaaggc cgaggagtga ccttcctatt tcctatacaa gcaaagacat
     2101 tccatcatgt ttacagcggg aaggacttaa ttgcacaggc acgggcagga actgggaaga
     2161 cattctcctt tgccatccct ttgattgaga aacttcatgg ggaactgcaa gacaggaaga
     2221 gaggccgtgc ccctcaggta ctggttcttg cacctacaag agagttggca aatcaagtaa
     2281 gcaaagactt cagtgacatc acaaaaaagc tgtcagtggc ttgtttttat ggtggaactc
     2341 cctatggagg tcaatttgaa cgcatgagga atgggattga tatcctggtt ggaacaccag
     2401 gtcgtatcaa agaccacata cagaatggca aactagatct caccaaactt aagcatgttg
     2461 tcctggatga agtggaccag atgttggata tgggatttgc tgatcaagtg gaagagattt
     2521 taagtgtggc atacaagaaa gattctgaag acaatcccca aacattgctt ttttctgcaa
     2581 cttgccctca ttgggtattt aatgttgcca agaaatacat gaaatctaca tatgaacagg
     2641 tggacctgat tggtaaaaag actcagaaaa cggcaataac tgtggagcat ctggctatta
     2701 agtgccactg gactcagagg gcagcagtta ttggggatgt catccgagta tatagtggtc
     2761 atcaaggacg cactatcatc ttttgtgaaa ccaagaaaga agcccaggag ctgtcccaga
     2821 attcagctat aaagcaggat gctcagtcct tgcatggaga cattccacag aagcaaaggg
     2881 aaatcaccct gaaaggtttt agaaatggta gttttggagt tttggtggca accaatgttg
     2941 ctgcacgtgg gttagacatc cctgaggttg atttggttat acaaagctct ccaccaaagg
     3001 atgtagagtc ctacattcat cgatccgggc ggacaggcag agctggaagg acgggggtgt
     3061 gcatctgctt ttatcagcac aaggaagaat atcagttagt acaagtggag caaaaagcgg
     3121 gaattaagtt caaacgaata ggtgttcctt ctgcaacaga aataataaaa gcttccagca
     3181 aagatgccat caggcttttg gattccgtgc ctcccactgc cattagtcac ttcaaacaat
     3241 cagctgagaa gctgatagag gagaagggag ctgtggaagc tctggcagca gcactggccc
     3301 atatttcagg tgccacgtcc gtagaccagc gctccttgat caactcaaat gtgggttttg
     3361 tgaccatgat cttgcagtgc tcaattgaaa tgccaaatat tagttatgct tggaaagaac
     3421 ttaaagagca gctgggcgag gagattgatt ccaaagtgaa gggaatggtt tttctcaaag
     3481 gaaagctggg tgtttgcttt gatgtaccta ccgcatcagt aacagaaata caggagaaat
     3541 ggcatgattc acgacgctgg cagctctctg tggccacaga gcaaccagaa ctggaaggac
     3601 cacgggaagg atatggaggc ttcaggggac agcgggaagg cagtcgaggc ttcaggggac
     3661 agcgggacgg aaacagaaga ttcagaggac agcgggaagg cagtagaggc ccgagaggac
     3721 agcgatcagg aggtggcaac aaaagtaaca gatcccaaaa caaaggccag aagcggagtt
     3781 tcagtaaagc atttggtcaa tacccaactt tcttgtacaa agtggttgat atccagcaca
     3841 gtggcggccg ctcgagtcta gagggcccgc ggttcgaaac cagcggaggc ggcggaagcg
     3901 ggggaggagg cagcggcgga ggcggcagcg ggggcggcgg aagcgctacc ggtaagccta
     3961 tccctaaccc tctcctcggt ctcgattcta cgcgtaccgg tatggcttct aactttactc
     4021 agttcgttct cgtcgacaat ggcggaactg gcgacgtgac tgtcgcccca agcaacttcg
     4081 ctaacggggt cgctgaatgg atcagctcta actcgcgatc acaggcttac aaagtaacct
     4141 gtagcgttcg tcagagctct gcgcagaatc gcaaatacac catcaaagtc gaggtgccta
     4201 aagtggcaac ccagactgtt ggtggtgaag agcttcctgt agccggatgg agatcttact
     4261 taaatatgga actaaccatt ccaattttcg ccacgaattc cgactgcgag cttattgtta
     4321 aggcaatgca aggtctccta aaagatggaa acccgattcc ctcggccatc gcggccaact
     4381 ccggcatcta cggaggtgga ggtggatctc gtacgggtta gtaatgagtt taaacccgct
     4441 gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc
     4501 cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg
     4561 catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca
     4621 agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt
     4681 ctgaggcgga aagaaccagc tggggctcta gggggtatcc ccacgcgccc tgtagcggcg
     4741 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
     4801 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
     4861 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
     4921 accccaaaaa acttgattag ggtgatggtt cacgtaccta gaagttccta ttccgaagtt
     4981 cctattctct agaaagtata ggaacttcct tggccaaaaa gcctgaactc accgcgacgt
     5041 ctgtcgagaa gtttctgatc gaaaagttcg acagcgtctc cgacctgatg cagctctcgg
     5101 agggcgaaga atctcgtgct ttcagcttcg atgtaggagg gcgtggatat gtcctgcggg
     5161 taaatagctg cgccgatggt ttctacaaag atcgttatgt ttatcggcac tttgcatcgg
     5221 ccgcgctccc gattccggaa gtgcttgaca ttggggaatt cagcgagagc ctgacctatt
     5281 gcatctcccg ccgtgcacag ggtgtcacgt tgcaagacct gcctgaaacc gaactgcccg
     5341 ctgttctgca gccggtcgcg gaggccatgg atgcgatcgc tgcggccgat cttagccaga
     5401 cgagcgggtt cggcccattc ggaccgcaag gaatcggtca atacactaca tggcgtgatt
     5461 tcatatgcgc gattgctgat ccccatgtgt atcactggca aactgtgatg gacgacaccg
     5521 tcagtgcgtc cgtcgcgcag gctctcgatg agctgatgct ttgggccgag gactgccccg
     5581 aagtccggca cctcgtgcac gcggatttcg gctccaacaa tgtcctgacg gacaatggcc
     5641 gcataacagc ggtcattgac tggagcgagg cgatgttcgg ggattcccaa tacgaggtcg
     5701 ccaacatctt cttctggagg ccgtggttgg cttgtatgga gcagcagacg cgctacttcg
     5761 agcggaggca tccggagctt gcaggatcgc cgcggctccg ggcgtatatg ctccgcattg
     5821 gtcttgacca actctatcag agcttggttg acggcaattt cgatgatgca gcttgggcgc
     5881 agggtcgatg cgacgcaatc gtccgatccg gagccgggac tgtcgggcgt acacaaatcg
     5941 cccgcagaag cgcggccgtc tggaccgatg gctgtgtaga agtactcgcc gatagtggaa
     6001 accgacgccc cagcactcgt ccgagggcaa aggaatagca cgtactacga gatttcgatt
     6061 ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga
     6121 tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg
     6181 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     6241 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta
     6301 taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa
     6361 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
     6421 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
     6481 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     6541 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     6601 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     6661 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     6721 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     6781 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     6841 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     6901 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     6961 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     7021 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     7081 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     7141 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     7201 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     7261 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     7321 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     7381 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     7441 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     7501 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     7561 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     7621 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     7681 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     7741 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     7801 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     7861 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     7921 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     7981 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     8041 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     8101 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     8161 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     8221 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     8281 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     8341 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     8401 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     8461 cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg atcgggagat ctcccgatcc
     8521 cctatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatctgct
     8581 ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag ctacaacaag
     8641 gcaaggcttg accgacaatt gcatgaagaa tctgcttagg gttaggcgtt ttgcgctgct
     8701 tcgcgatgta
//
