LOCUS       sequence-376729-        8947 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For use in RBP tethering screen.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8947)
  AUTHORS   Luo EC, Nathanson JL, Tan FE, Schwartz JL, Schmok JC, Shankar A, 
            Markmiller S, Yee BA, Sathe S, Pratt GA, Scaletta DB, Ha Y, Hill DE,
            Aigner S, Yeo GW
  TITLE     Large-scale tethered function assays identify factors that regulate 
            mRNA stability and translation.
  JOURNAL   Nat Struct Mol Biol. 2020 Oct;27(10):989-1000. doi: 
            10.1038/s41594-020-0477-6. Epub 2020 Aug 17.
  PUBMED    32807991
REFERENCE   2  (bases 1 to 8947)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41594-020-0477-6"; journalName: "Nat Struct Mol Biol"; 
            date: "2020-10"; volume: "27"; issue: "10"; pages: "989-1000"
COMMENT     Sequence Label: DDX24
FEATURES             Location/Qualifiers
     source          1..8947
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        144..1322
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          375..1313
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1270..1290
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     primer_bind     1339..1358
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1339..1357
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1436..1460
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     RBS             3072..3080
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     protein_bind    complement(4047..4071)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4196..4237
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(4692..4709)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4698..4922
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5055..5074)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    5206..5253
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             5261..6283
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             5261..6283
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    6413..6534
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6450..6469)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6504..6523
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6583..6599)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6583..6599)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6596..6618)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6607..6623
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6631..6637,6638..6655,6656..6661))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6676..6697
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6814..6831)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6985..7573)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7065..7084)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7744..8535,8536..8604))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8367..8386
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8605..8709)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8784..8803)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gcgatgtacg ggccagatat acgcgttgac attgattatt gactaggctt ttgcaaaaag
       61 ctttgcaaag atggataaag ttttaaacag agaggaatct ttgcagctaa tggaccttct
      121 aggtcttgaa aggagtggga attggctccg gtgcccgtca gtgggcagag cgcacatcgc
      181 ccacagtccc cgagaagttg gggggagggg tcggcaattg aaccggtgcc tagagaaggt
      241 ggcgcggggt aaactgggaa agtgatgtcg tgtactggct ccgccttttt cccgagggtg
      301 ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg
      361 ccgccagaac acaggtaagt gccgtgtgtg gttcccgcgg gcctggcctc tttacgggtt
      421 atggcccttg cgtgccttga attacttcca cctggctgca gtacgtgatt cttgatcccg
      481 agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga gccccttcgc
      541 ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga atctggtggc
      601 accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat ttttgatgac
      661 ctgctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa gatctgcaca
      721 ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc cagcgcacat
      781 gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg tagtctcaag
      841 ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg ccctgggcgg
      901 caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt cccggccctg
      961 ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt gagtcaccca
     1021 cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc acggagtacc
     1081 gggcgccgtc caggcacctc gattagttct cgagcttttg gagtacgtcg tctttaggtt
     1141 ggggggaggg gttttatgcg atggagtttc cccacactga gtgggtggag actgaagtta
     1201 ggccagcttg gcacttgatg taattctcct tggaatttgc cctttttgag tttggatctt
     1261 ggttcattct caagcctcag acagtggttc aaagtttttt tcttccattt caggtgtcgt
     1321 gaggaattag cttggtacta atacgactca ctatagggag acccaagctg gctaggtaag
     1381 cttggtaccg agctcggatc cactagtcca gtgtggtgga attctgcaga tatcaacaag
     1441 tttgtacaaa aaagcaggct ccaccatgaa gttgaaggac acaaaatcaa ggccaaagca
     1501 gtcaagctgt ggcaaatttc agacaaaggg aatcaaagtt gtgggaaaat ggaaggaagt
     1561 gaagattgac ccaaatatgt ttgcagatgg acagatggat gacttggtgt gctttgagga
     1621 attgacagat taccagttgg tctcccctgc caagaatccc tccagtctct tctcaaagga
     1681 agcacccaag agaaaggcac aagctgtttc agaagaagag gaggaggagg agggaaagtc
     1741 tagctcacca aagaaaaaga tcaagttgaa gaaaagtaaa aatgtagcaa ctgaaggaac
     1801 cagtacccag aaagaatttg aagtgaaaga tcctgagctg gaggcccagg gagatgacat
     1861 ggtttgtgat gatccggagg ctggggagat gacatcagaa aacctggtcc aaactgctcc
     1921 aaaaaagaag aaaaataaag ggaaaaaagg gttggagcct tctcagagca ctgctgccaa
     1981 ggtgcccaaa aaagcgaaga catggattcc tgaagttcat gatcagaaag cagatgtgtc
     2041 agcttggaag gacctgtttg ttcccaggcc ggttctccga gcactcagct ttctaggctt
     2101 ctctgcaccc acaccaatcc aagccctgac cttggcacct gccatccgtg acaaactgga
     2161 catccttggg gctgctgaga caggaagtgg gaaaactctt gcctttgcca tcccaatgat
     2221 tcatgcggtg ttgcagtggc agaagaggaa tgctgcccct cctccaagta acaccgaagc
     2281 accacctgga gagaccagaa ctgaggccgg agctgagact agatcaccag gcaaggctga
     2341 agctgagtct gatgcattgc ctgacgatac tgtaattgag agtgaagcac tgcccagtga
     2401 tattgcagcc gaggccagag ccaagactgg aggcactgtc tcagaccagg cgttgctctt
     2461 tggtgacgat gatgctggtg aagggccttc ttccctgatc agggagaaac ctgttcccaa
     2521 acagaatgag aatgaggagg aaaatcttga taaagagcag actggaaatc taaaacagga
     2581 gttggatgac aaaagcgcca cctgtaaggc atatccaaag cgtcctctgc ttggactggt
     2641 tctgactccc actcgagagc tggccgtcca ggtcaaacag cacattgatg ctgtggccag
     2701 gtttacagga attaaaactg ctattttggt tggtggaatg tccacgcaga aacagcagag
     2761 gatgctgaac cgtcgtcctg agattgtggt tgctactcca ggccggctgt gggaattaat
     2821 taaagaaaag cattatcatt tgaggaacct tcggcagctc aggtgcctgg tagtggatga
     2881 ggctgaccgg atggttgaga aaggccattt tgctgagctc tcacagctgc tagagatgct
     2941 caatgactcc caatacaacc caaagagaca aacgcttgtt ttttctgcca cactcaccct
     3001 ggtgcatcag gctcctgctc gaatccttca taagaagcac accaagaaaa tggataaaac
     3061 agccaaactt gacctcctta tgcagaaaat tggcatgagg ggcaagccca aggtcattga
     3121 cctcacaagg aatgaggcca cggtggagac gctaacagag accaagatcc attgtgagac
     3181 tgatgagaaa gacttctact tgtactactt cctgatgcag tatccaggcc gcagcttagt
     3241 gtttgccaac agtatctcct gcatcaaacg cctctctggg ctcctcaaag tccttgatat
     3301 catgcccttg accctgcatg cctgtatgca ccagaagcag aggctcagaa acctggagca
     3361 gtttgcccgt ctggaagact gtgttctctt ggcaacagat gtggcggctc ggggtctgga
     3421 tattcctaaa gtccagcatg tcatccatta ccaggtccca cgtacctcgg agatttatgt
     3481 ccaccgaagt ggtcgaactg ctcgagctac caatgaaggc ctcagtctga tgctcattgg
     3541 gcctgaggat gtgatcaact ttaagaagat ttacaaaacg ctcaagaaag atgaggatat
     3601 cccactgttc cccgtgcaga caaaatacat ggatgtggtc aaggagcgaa tccgtttagc
     3661 tcgacagatt gagaaatctg agtatcggaa cttccaggct tgcctgcaca actcttggat
     3721 tgagcaggca gcagctgccc tggagattga gctggaagaa gacatgtata agggaggaaa
     3781 agctgaccag caagaagaac gtcggagaca aaagcagatg aaggttctga agaaggagct
     3841 gcgccacctg ctgtcccagc cactgtttac ggagagccag aaaaccaagt atcccactca
     3901 gtctggcaag ccgcccctgc ttgtgtctgc cccaagtaag agcgagtctg ctttgagctg
     3961 tctctccaag cagaagaaga agaagacaaa gaagccgaag gagccacagc cggaacagcc
     4021 acagccaagt acaagtgcaa atttggaccc agctttcttg tacaaagtgg ttgatatcca
     4081 gcacagtggc ggccgctcga gtctagaggg cccgcggttc gaaaccagcg gaggcggcgg
     4141 aagcggggga ggaggcagcg gcggaggcgg cagcgggggc ggcggaagcg ctaccggtaa
     4201 gcctatccct aaccctctcc tcggtctcga ttctacgcgt accggtatgg cttctaactt
     4261 tactcagttc gttctcgtcg acaatggcgg aactggcgac gtgactgtcg ccccaagcaa
     4321 cttcgctaac ggggtcgctg aatggatcag ctctaactcg cgatcacagg cttacaaagt
     4381 aacctgtagc gttcgtcaga gctctgcgca gaatcgcaaa tacaccatca aagtcgaggt
     4441 gcctaaagtg gcaacccaga ctgttggtgg tgaagagctt cctgtagccg gatggagatc
     4501 ttacttaaat atggaactaa ccattccaat tttcgccacg aattccgact gcgagcttat
     4561 tgttaaggca atgcaaggtc tcctaaaaga tggaaacccg attccctcgg ccatcgcggc
     4621 caactccggc atctacggag gtggaggtgg atctcgtacg ggttagtaat gagtttaaac
     4681 ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc
     4741 cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga
     4801 aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga
     4861 cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat
     4921 ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag
     4981 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     5041 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     5101 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     5161 cctcgacccc aaaaaacttg attagggtga tggttcacgt acctagaagt tcctattccg
     5221 aagttcctat tctctagaaa gtataggaac ttccttggcc aaaaagcctg aactcaccgc
     5281 gacgtctgtc gagaagtttc tgatcgaaaa gttcgacagc gtctccgacc tgatgcagct
     5341 ctcggagggc gaagaatctc gtgctttcag cttcgatgta ggagggcgtg gatatgtcct
     5401 gcgggtaaat agctgcgccg atggtttcta caaagatcgt tatgtttatc ggcactttgc
     5461 atcggccgcg ctcccgattc cggaagtgct tgacattggg gaattcagcg agagcctgac
     5521 ctattgcatc tcccgccgtg cacagggtgt cacgttgcaa gacctgcctg aaaccgaact
     5581 gcccgctgtt ctgcagccgg tcgcggaggc catggatgcg atcgctgcgg ccgatcttag
     5641 ccagacgagc gggttcggcc cattcggacc gcaaggaatc ggtcaataca ctacatggcg
     5701 tgatttcata tgcgcgattg ctgatcccca tgtgtatcac tggcaaactg tgatggacga
     5761 caccgtcagt gcgtccgtcg cgcaggctct cgatgagctg atgctttggg ccgaggactg
     5821 ccccgaagtc cggcacctcg tgcacgcgga tttcggctcc aacaatgtcc tgacggacaa
     5881 tggccgcata acagcggtca ttgactggag cgaggcgatg ttcggggatt cccaatacga
     5941 ggtcgccaac atcttcttct ggaggccgtg gttggcttgt atggagcagc agacgcgcta
     6001 cttcgagcgg aggcatccgg agcttgcagg atcgccgcgg ctccgggcgt atatgctccg
     6061 cattggtctt gaccaactct atcagagctt ggttgacggc aatttcgatg atgcagcttg
     6121 ggcgcagggt cgatgcgacg caatcgtccg atccggagcc gggactgtcg ggcgtacaca
     6181 aatcgcccgc agaagcgcgg ccgtctggac cgatggctgt gtagaagtac tcgccgatag
     6241 tggaaaccga cgccccagca ctcgtccgag ggcaaaggaa tagcacgtac tacgagattt
     6301 cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg
     6361 ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt
     6421 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     6481 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
     6541 ctgtataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt
     6601 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
     6661 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
     6721 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
     6781 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     6841 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     6901 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     6961 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     7021 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     7081 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     7141 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     7201 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     7261 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     7321 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     7381 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
     7441 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     7501 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     7561 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     7621 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     7681 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     7741 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     7801 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     7861 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     7921 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     7981 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     8041 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     8101 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     8161 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     8221 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     8281 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     8341 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     8401 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     8461 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     8521 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     8581 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     8641 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     8701 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc gacggatcgg gagatctccc
     8761 gatcccctat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat
     8821 ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat ttaagctaca
     8881 acaaggcaag gcttgaccga caattgcatg aagaatctgc ttagggttag gcgttttgcg
     8941 ctgcttc
//
