LOCUS       sequence-37673-e         707 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 707)
  AUTHORS   Mukherji S, Ebert MS, Zheng GX, Tsang JS, Sharp PA, van Oudenaarden 
            A
  TITLE     MicroRNAs can generate thresholds in target gene expression.
  JOURNAL   Nat Genet. 2011 Aug 21;43(9):854-9. doi: 10.1038/ng.905.
  PUBMED    21857679
REFERENCE   2  (bases 1 to 707)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ng"; 
            journalName: "Nat Genet"; date: "2011-08-21- 21"; volume: "43"; 
            issue: "9"; pages: "854-9"
COMMENT     Sequence Label: pTRETightBI-RY-1pf
FEATURES             Location/Qualifiers
     source          1..707
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    401..482
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(438..457)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        515..619
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aggaacggcg ccctgaaggg cgagatcaag cagaggctga agctgaagga cggcggccac
       61 tacgacgctg aggtcaagac cacctacaag gccaagaagc ccgtgcagct gcccggcgcc
      121 tacaacgtca acatcaagtt ggacatcacc tcccacaacg aggactacac catcgtggaa
      181 cagtacgaac gcgccgaggg ccgccactcc accggcggca tggacgagct gtacaagtaa
      241 atcgataagc ttctacctgc actataagca ctttagtcga cgatatctcc agaggatcat
      301 aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc
      361 cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta
      421 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
      481 gccgacgtca ggtggcactt ttcggggaan tgtgcgcgga acccctattt gtttattttt
      541 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
      601 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
      661 tgcggcattt tgccttcctg tttttgctca cncagaaacg ctggtga
//
