LOCUS       sequence-376731-        8656 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For use in RBP tethering screen.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8656)
  AUTHORS   Luo EC, Nathanson JL, Tan FE, Schwartz JL, Schmok JC, Shankar A, 
            Markmiller S, Yee BA, Sathe S, Pratt GA, Scaletta DB, Ha Y, Hill DE,
            Aigner S, Yeo GW
  TITLE     Large-scale tethered function assays identify factors that regulate 
            mRNA stability and translation.
  JOURNAL   Nat Struct Mol Biol. 2020 Oct;27(10):989-1000. doi: 
            10.1038/s41594-020-0477-6. Epub 2020 Aug 17.
  PUBMED    32807991
REFERENCE   2  (bases 1 to 8656)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41594-020-0477-6"; journalName: "Nat Struct Mol Biol"; 
            date: "2020-10"; volume: "27"; issue: "10"; pages: "989-1000"
COMMENT     Sequence Label: DDX27
FEATURES             Location/Qualifiers
     source          1..8656
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        157..1335
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          388..1326
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1283..1303
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     primer_bind     1352..1371
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1352..1370
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1449..1473
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3769..3793)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3918..3959
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(4414..4431)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4420..4644
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(4777..4796)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    4928..4975
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             4983..6005
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             4983..6005
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    6135..6256
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6172..6191)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6226..6245
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6305..6321)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6305..6321)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6318..6340)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6329..6345
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6353..6359,6360..6377,6378..6383))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6398..6419
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6536..6553)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6707..7295)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6787..6806)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7466..8257,8258..8326))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8089..8108
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8327..8431)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8506..8525)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 tttgcgctgc ttcgcgatgt acgggccaga tatacgcgtt gacattgatt attgactagg
       61 cttttgcaaa aagctttgca aagatggata aagttttaaa cagagaggaa tctttgcagc
      121 taatggacct tctaggtctt gaaaggagtg ggaattggct ccggtgcccg tcagtgggca
      181 gagcgcacat cgcccacagt ccccgagaag ttggggggag gggtcggcaa ttgaaccggt
      241 gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt
      301 tttcccgagg gtgggggaga accgtatata agtgcagtag tcgccgtgaa cgttcttttt
      361 cgcaacgggt ttgccgccag aacacaggta agtgccgtgt gtggttcccg cgggcctggc
      421 ctctttacgg gttatggccc ttgcgtgcct tgaattactt ccacctggct gcagtacgtg
      481 attcttgatc ccgagcttcg ggttggaagt gggtgggaga gttcgaggcc ttgcgcttaa
      541 ggagcccctt cgcctcgtgc ttgagttgag gcctggcctg ggcgctgggg ccgccgcgtg
      601 cgaatctggt ggcaccttcg cgcctgtctc gctgctttcg ataagtctct agccatttaa
      661 aatttttgat gacctgctgc gacgcttttt ttctggcaag atagtcttgt aaatgcgggc
      721 caagatctgc acactggtat ttcggttttt ggggccgcgg gcggcgacgg ggcccgtgcg
      781 tcccagcgca catgttcggc gaggcggggc ctgcgagcgc ggccaccgag aatcggacgg
      841 gggtagtctc aagctggccg gcctgctctg gtgcctggcc tcgcgccgcc gtgtatcgcc
      901 ccgccctggg cggcaaggct ggcccggtcg gcaccagttg cgtgagcgga aagatggccg
      961 cttcccggcc ctgctgcagg gagctcaaaa tggaggacgc ggcgctcggg agagcgggcg
     1021 ggtgagtcac ccacacaaag gaaaagggcc tttccgtcct cagccgtcgc ttcatgtgac
     1081 tccacggagt accgggcgcc gtccaggcac ctcgattagt tctcgagctt ttggagtacg
     1141 tcgtctttag gttgggggga ggggttttat gcgatggagt ttccccacac tgagtgggtg
     1201 gagactgaag ttaggccagc ttggcacttg atgtaattct ccttggaatt tgcccttttt
     1261 gagtttggat cttggttcat tctcaagcct cagacagtgg ttcaaagttt ttttcttcca
     1321 tttcaggtgt cgtgaggaat tagcttggta ctaatacgac tcactatagg gagacccaag
     1381 ctggctaggt aagcttggta ccgagctcgg atccactagt ccagtgtggt ggaattctgc
     1441 agatatcaac aagtttgtac aaaaaagttg gcatgcttgc ggacctcggc ttaatcggaa
     1501 ccataggcga ggatgacgag gtgccggtgg agcccgagtc tgactccggg gacgaggaag
     1561 aggaggggcc cattgtgctg ggcagacgac aaaaagcttt ggggaagaac cgcagtgctg
     1621 atttcaaccc tgatttcgtt ttcactgaga aggaggggac gtacgatggc agctgggccc
     1681 tggctgatgt catgagccaa ctcaagaaga agagggcagc cactacatta gatgagaaga
     1741 ttgagaaagt tcgaaagaaa aggaaaacag aggataaaga agccaagtct gggaagttgg
     1801 aaaaggagaa agaagcaaag gaaggctctg aaccaaagga gcaggaagac cttcaagaga
     1861 atgatgagga aggctcagaa gatgaagcct cggagactga ctactcatca gctgatgaga
     1921 acatcctcac caaagcagat acactcaaag taaaggatcg gaagaagaag aagaagaaag
     1981 gacaggaagc aggaggattt tttgaagatg catctcagta cgatgaaaac ctctcgttcc
     2041 aggacatgaa cctttcccgc cctcttctga aggccattac agccatgggc ttcaagcagc
     2101 ccaccccgat ccagaaggcg tgcatacctg tgggtctatt ggggaaggac atctgtgcct
     2161 gtgcagccac tgggacaggt aaaactgccg cctttgccct gcctgttttg gagcgtctga
     2221 tttataaacc ccgccaggct ccagtcaccc gcgtgctggt gctagtgccc acccgagagc
     2281 tgggcatcca ggtgcactct gtcaccagac agctggccca gttctgcaac atcaccacct
     2341 gcctggctgt gggcggcttg gatgtgaagt ctcaggaagc agctcttcgg gcagcgcctg
     2401 acatcctcat cgccacccca ggccggctca tcgatcacct ccacaactgc ccttccttcc
     2461 acctgagcag catcgaggtg ctcatcctgg acgaggctga caggatgctg gatgagtact
     2521 ttgaggagca gatgaaggag atcatccgaa tgtgttccca ccaccgccag accatgctct
     2581 tctcggccac catgacagac gaggtgaaag atctggcttc tgtctccttg aagaatcctg
     2641 tccggatatt tgtgaacagc aacacagatg tggctccctt tctgcggcag gagttcatcc
     2701 ggatccggcc taatcgtgaa ggagaccggg aagccatcgt ggcagctttg ttgacgagga
     2761 ccttcactga ccatgtgatg ctgttcacgc aaaccaagaa gcaggcccac cgcatgcaca
     2821 tcctcctggg gctcatgggg ctgcaggtgg gtgagctcca tggcaacttg tcacagacgc
     2881 agcggctgga ggccctccgg cgttttaagg atgaacagat tgacatcctc gtggccactg
     2941 atgtggcagc ccgtggactt gacattgagg gggtcaaaac ggtaatcaac ttcacaatgc
     3001 ctaataccat caaacattat gtccaccggg tggggcgaac agcacgtgct ggcagggctg
     3061 ggcgctcagt ctctctggtg ggagaagatg agcggaagat gctgaaggag attgtaaaag
     3121 ctgccaaggc ccctgtgaag gccaggatac ttccccaaga tgtcatcctc aaattccggg
     3181 acaagattga gaaaatggag aaagatgtgt atgcagttct gcagctagag gcggaggaaa
     3241 aagagatgca gcagtcagaa gcccagatca atacagcaaa gcggctcctg gagaagggga
     3301 aggaggcagt ggtccaagag cccgagagga gctggttcca gaccaaagaa gagaggaaga
     3361 aggagaaaat tgccaaagct ctgcaggaat ttgacttggc cttaagagga aagaagaaaa
     3421 ggaagaagtt tatgaaggat gccaaaaaaa agggggagat gacagcagag gaaaggtctc
     3481 agtttgaaat cctcaaggcg cagatgtttg ctgaacggct agcgaagagg aatcgcagag
     3541 ccaagcgggc ccgagcaatg cccgaggagg agccagtgag aggtcctgcc aagaagcaaa
     3601 agcaggggaa gaaatctgta tttgatgaag aactcaccaa cacaagcaag aaggccctga
     3661 aacagtatcg agctagccct tcctttgaag aaaggaaaca gttgggcttg ccccaccaga
     3721 gacgaggagg aaactttaaa tctaaatcca gatacaagag gaggaagtac ccaactttct
     3781 tgtacaaagt ggttgatatc cagcacagtg gcggccgctc gagtctagag ggcccgcggt
     3841 tcgaaaccag cggaggcggc ggaagcgggg gaggaggcag cggcggaggc ggcagcgggg
     3901 gcggcggaag cgctaccggt aagcctatcc ctaaccctct cctcggtctc gattctacgc
     3961 gtaccggtat ggcttctaac tttactcagt tcgttctcgt cgacaatggc ggaactggcg
     4021 acgtgactgt cgccccaagc aacttcgcta acggggtcgc tgaatggatc agctctaact
     4081 cgcgatcaca ggcttacaaa gtaacctgta gcgttcgtca gagctctgcg cagaatcgca
     4141 aatacaccat caaagtcgag gtgcctaaag tggcaaccca gactgttggt ggtgaagagc
     4201 ttcctgtagc cggatggaga tcttacttaa atatggaact aaccattcca attttcgcca
     4261 cgaattccga ctgcgagctt attgttaagg caatgcaagg tctcctaaaa gatggaaacc
     4321 cgattccctc ggccatcgcg gccaactccg gcatctacgg aggtggaggt ggatctcgta
     4381 cgggttagta atgagtttaa acccgctgat cagcctcgac tgtgccttct agttgccagc
     4441 catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg
     4501 tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc
     4561 tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg
     4621 ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctctaggg
     4681 ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
     4741 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
     4801 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
     4861 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac
     4921 gtacctagaa gttcctattc cgaagttcct attctctaga aagtatagga acttccttgg
     4981 ccaaaaagcc tgaactcacc gcgacgtctg tcgagaagtt tctgatcgaa aagttcgaca
     5041 gcgtctccga cctgatgcag ctctcggagg gcgaagaatc tcgtgctttc agcttcgatg
     5101 taggagggcg tggatatgtc ctgcgggtaa atagctgcgc cgatggtttc tacaaagatc
     5161 gttatgttta tcggcacttt gcatcggccg cgctcccgat tccggaagtg cttgacattg
     5221 gggaattcag cgagagcctg acctattgca tctcccgccg tgcacagggt gtcacgttgc
     5281 aagacctgcc tgaaaccgaa ctgcccgctg ttctgcagcc ggtcgcggag gccatggatg
     5341 cgatcgctgc ggccgatctt agccagacga gcgggttcgg cccattcgga ccgcaaggaa
     5401 tcggtcaata cactacatgg cgtgatttca tatgcgcgat tgctgatccc catgtgtatc
     5461 actggcaaac tgtgatggac gacaccgtca gtgcgtccgt cgcgcaggct ctcgatgagc
     5521 tgatgctttg ggccgaggac tgccccgaag tccggcacct cgtgcacgcg gatttcggct
     5581 ccaacaatgt cctgacggac aatggccgca taacagcggt cattgactgg agcgaggcga
     5641 tgttcgggga ttcccaatac gaggtcgcca acatcttctt ctggaggccg tggttggctt
     5701 gtatggagca gcagacgcgc tacttcgagc ggaggcatcc ggagcttgca ggatcgccgc
     5761 ggctccgggc gtatatgctc cgcattggtc ttgaccaact ctatcagagc ttggttgacg
     5821 gcaatttcga tgatgcagct tgggcgcagg gtcgatgcga cgcaatcgtc cgatccggag
     5881 ccgggactgt cgggcgtaca caaatcgccc gcagaagcgc ggccgtctgg accgatggct
     5941 gtgtagaagt actcgccgat agtggaaacc gacgccccag cactcgtccg agggcaaagg
     6001 aatagcacgt actacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg
     6061 gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt
     6121 tcttcgccca ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     6181 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     6241 tcatcaatgt atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat
     6301 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     6361 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
     6421 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
     6481 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     6541 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     6601 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     6661 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     6721 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     6781 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     6841 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     6901 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     6961 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     7021 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     7081 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     7141 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     7201 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     7261 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     7321 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     7381 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     7441 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     7501 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     7561 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     7621 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     7681 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     7741 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     7801 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     7861 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     7921 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     7981 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     8041 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     8101 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     8161 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     8221 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     8281 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     8341 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     8401 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
     8461 tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga
     8521 tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg
     8581 cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct
     8641 gcttagggtt aggcgt
//
