LOCUS       sequence-376733-        8752 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For use in RBP tethering screen.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8752)
  AUTHORS   Luo EC, Nathanson JL, Tan FE, Schwartz JL, Schmok JC, Shankar A, 
            Markmiller S, Yee BA, Sathe S, Pratt GA, Scaletta DB, Ha Y, Hill DE,
            Aigner S, Yeo GW
  TITLE     Large-scale tethered function assays identify factors that regulate 
            mRNA stability and translation.
  JOURNAL   Nat Struct Mol Biol. 2020 Oct;27(10):989-1000. doi: 
            10.1038/s41594-020-0477-6. Epub 2020 Aug 17.
  PUBMED    32807991
REFERENCE   2  (bases 1 to 8752)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41594-020-0477-6"; journalName: "Nat Struct Mol Biol"; 
            date: "2020-10"; volume: "27"; issue: "10"; pages: "989-1000"
COMMENT     Sequence Label: DDX27-1
FEATURES             Location/Qualifiers
     source          1..8752
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        137..1315
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          368..1306
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1263..1283
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     primer_bind     1332..1351
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1332..1350
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1429..1453
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3845..3869)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3994..4035
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(4490..4507)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4496..4720
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(4853..4872)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    5004..5051
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             5059..6081
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             5059..6081
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    6211..6332
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6248..6267)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6302..6321
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6381..6397)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6381..6397)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6394..6416)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6405..6421
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6429..6435,6436..6453,6454..6459))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6474..6495
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6612..6629)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6783..7371)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6863..6882)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7542..8333,8334..8402))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8165..8184
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8403..8507)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8582..8601)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 acgggccaga tatacgcgtt gacattgatt attgactagg cttttgcaaa aagctttgca
       61 aagatggata aagttttaaa cagagaggaa tctttgcagc taatggacct tctaggtctt
      121 gaaaggagtg ggaattggct ccggtgcccg tcagtgggca gagcgcacat cgcccacagt
      181 ccccgagaag ttggggggag gggtcggcaa ttgaaccggt gcctagagaa ggtggcgcgg
      241 ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg gtgggggaga
      301 accgtatata agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag
      361 aacacaggta agtgccgtgt gtggttcccg cgggcctggc ctctttacgg gttatggccc
      421 ttgcgtgcct tgaattactt ccacctggct gcagtacgtg attcttgatc ccgagcttcg
      481 ggttggaagt gggtgggaga gttcgaggcc ttgcgcttaa ggagcccctt cgcctcgtgc
      541 ttgagttgag gcctggcctg ggcgctgggg ccgccgcgtg cgaatctggt ggcaccttcg
      601 cgcctgtctc gctgctttcg ataagtctct agccatttaa aatttttgat gacctgctgc
      661 gacgcttttt ttctggcaag atagtcttgt aaatgcgggc caagatctgc acactggtat
      721 ttcggttttt ggggccgcgg gcggcgacgg ggcccgtgcg tcccagcgca catgttcggc
      781 gaggcggggc ctgcgagcgc ggccaccgag aatcggacgg gggtagtctc aagctggccg
      841 gcctgctctg gtgcctggcc tcgcgccgcc gtgtatcgcc ccgccctggg cggcaaggct
      901 ggcccggtcg gcaccagttg cgtgagcgga aagatggccg cttcccggcc ctgctgcagg
      961 gagctcaaaa tggaggacgc ggcgctcggg agagcgggcg ggtgagtcac ccacacaaag
     1021 gaaaagggcc tttccgtcct cagccgtcgc ttcatgtgac tccacggagt accgggcgcc
     1081 gtccaggcac ctcgattagt tctcgagctt ttggagtacg tcgtctttag gttgggggga
     1141 ggggttttat gcgatggagt ttccccacac tgagtgggtg gagactgaag ttaggccagc
     1201 ttggcacttg atgtaattct ccttggaatt tgcccttttt gagtttggat cttggttcat
     1261 tctcaagcct cagacagtgg ttcaaagttt ttttcttcca tttcaggtgt cgtgaggaat
     1321 tagcttggta ctaatacgac tcactatagg gagacccaag ctggctaggt aagcttggta
     1381 ccgagctcgg atccactagt ccagtgtggt ggaattctgc agatatcaac aagtttgtac
     1441 aaaaaagttg gcaccatggt acttgcgcaa agacgacgag gaggctgcga aaagttaagg
     1501 gccggaccgc aggctgtgct cgcttccgga agtggcttct gcgacaacat gcttgcggac
     1561 ctcggcttaa tcggaaccat aggcgaggat gacgaggtgc cggtggagcc cgagtctgac
     1621 tccggggacg aggaagagga ggggcccatt gtgctgggca gacgacaaaa agctttgggg
     1681 aagaaccgca gtgctgattt caaccctgat ttcgttttca ctgagaagga ggggacgtac
     1741 gatggcagct gggccctggc tgatgtcatg agccaactca agaagaagag ggcagccact
     1801 acattagatg agaagattga gaaagttcga aagaaaagga aaacagagga taaagaagcc
     1861 aagtctggga agttggaaaa ggagaaagaa gcaaaggaag gctctgaacc aaaggagcag
     1921 gaagaccttc aagagaatga tgaggaaggc tcagaagatg aagcctcgga gactgactac
     1981 tcatcagctg atgagaacat cctcaccaaa gcagatacac tcaaagtaaa ggatcggaag
     2041 aagaagaaga agaaaggaca ggaagcagga ggattttttg aagatgcatc tcagtacgat
     2101 gaaaacctct cgttccagga catgaacctt tcccgccctc ttctgaaggc cattacagcc
     2161 atgggcttca agcagcccac cccgatccag aaggcgtgca tacctgtggg tctattgggg
     2221 aaggacatct gtgcctgtgc agccactggg acaggtaaaa ctgccgcctt tgccctgcct
     2281 gttttggagc gtctgattta taaaccccgc caggctccag tcacccgcgt gctggtgcta
     2341 gtgcccaccc gagagctggg catccaggtg cactctgtca ccagacagct ggcccagttc
     2401 tgcaacatca ccacctgcct ggctgtgggc ggcttggatg tgaagtctca ggaagcagct
     2461 cttcgggcag cgcctgacat cctcatcgcc accccaggcc ggctcatcga tcacctccac
     2521 aactgccctt ccttccacct gagcagcatc gaggtgctca tcctggacga ggctgacagg
     2581 atgctggatg agtactttga ggagcagatg aaggagatca tccgaatgtg ttcccaccac
     2641 cgccagacca tgctcttctc ggccaccatg acagacgagg tgaaagatct ggcttctgtc
     2701 tccttgaaga atcctgtccg gatatttgtg aacagcaaca cagatgtggc tcccttcctg
     2761 cggcaggagt tcatccggat ccggcctaat cgtgaaggag accgggaagc catcgtggca
     2821 gctttgttga cgaggacctt cactgaccat gtgatgctgt tcacgcaaac caagaagcag
     2881 gcccaccgca tgcacatcct cctggggctc atggggctgc aggtgggtga gctccatggc
     2941 aacttgtcac agacgcagcg gctggaggcc ctccggcgtt ttaaggatga acagattgac
     3001 atcctcgtgg ccactgatgt ggcagcccgt ggacttgaca ttgagggggt caaaacggta
     3061 atcaacttca caatgcctaa taccatcaaa cattatgtcc accgggtggg gcgaacagca
     3121 cgtgctggca gggctgggcg ctcagtctct ctggtgggag aagatgagcg gaagatgctg
     3181 aaggagattg taaaagctgc caaggcccct gtgaaggcca ggatacttcc ccaagatgtc
     3241 atcctcaaat tccgggacaa gattgagaaa atggagaaag atgtgtatgc agttctgcag
     3301 ctagaggcgg aggaaaaaga gatgcagcag tcagaagccc agatcaatac agcaaagcgg
     3361 ctcctggaga aggggaagga ggcagtggtc caagagcccg agaggagctg gttccagacc
     3421 aaagaagaga ggaagaagga gaaaattgcc aaagctctgc aggaatttga cttggcctta
     3481 agaggaaaga agaaaaggaa gaagtttatg aaggatgcca aaaaaaaggg ggagatgaca
     3541 gcagaggaaa ggtctcagtt tgaaatcctc aaggcgcaga tgtttgctga acggctagcg
     3601 aagaggaatc gcagagccaa gcgggcccga gcaatgcccg aggaggagcc agtgagaggt
     3661 cctgccaaga agcaaaagca ggggaagaaa tctgtatttg atgaagaact caccaacaca
     3721 agcaagaagg ccctgaaaca gtatcgagct ggcccttcct ttgaagaaag gaaacagttg
     3781 ggcttgcccc accagagacg aggaggaaac tttaaatcta aatccagata caagaggagg
     3841 aagttgccaa ctttcttgta caaagtggtt gatatccagc acagtggcgg ccgctcgagt
     3901 ctagagggcc cgcggttcga aaccagcgga ggcggcggaa gcgggggagg aggcagcggc
     3961 ggaggcggca gcgggggcgg cggaagcgct accggtaagc ctatccctaa ccctctcctc
     4021 ggtctcgatt ctacgcgtac cggtatggct tctaacttta ctcagttcgt tctcgtcgac
     4081 aatggcggaa ctggcgacgt gactgtcgcc ccaagcaact tcgctaacgg ggtcgctgaa
     4141 tggatcagct ctaactcgcg atcacaggct tacaaagtaa cctgtagcgt tcgtcagagc
     4201 tctgcgcaga atcgcaaata caccatcaaa gtcgaggtgc ctaaagtggc aacccagact
     4261 gttggtggtg aagagcttcc tgtagccgga tggagatctt acttaaatat ggaactaacc
     4321 attccaattt tcgccacgaa ttccgactgc gagcttattg ttaaggcaat gcaaggtctc
     4381 ctaaaagatg gaaacccgat tccctcggcc atcgcggcca actccggcat ctacggaggt
     4441 ggaggtggat ctcgtacggg ttagtaatga gtttaaaccc gctgatcagc ctcgactgtg
     4501 ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa
     4561 ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt
     4621 aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa
     4681 gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc
     4741 agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt
     4801 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
     4861 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     4921 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     4981 tagggtgatg gttcacgtac ctagaagttc ctattccgaa gttcctattc tctagaaagt
     5041 ataggaactt ccttggccaa aaagcctgaa ctcaccgcga cgtctgtcga gaagtttctg
     5101 atcgaaaagt tcgacagcgt ctccgacctg atgcagctct cggagggcga agaatctcgt
     5161 gctttcagct tcgatgtagg agggcgtgga tatgtcctgc gggtaaatag ctgcgccgat
     5221 ggtttctaca aagatcgtta tgtttatcgg cactttgcat cggccgcgct cccgattccg
     5281 gaagtgcttg acattgggga attcagcgag agcctgacct attgcatctc ccgccgtgca
     5341 cagggtgtca cgttgcaaga cctgcctgaa accgaactgc ccgctgttct gcagccggtc
     5401 gcggaggcca tggatgcgat cgctgcggcc gatcttagcc agacgagcgg gttcggccca
     5461 ttcggaccgc aaggaatcgg tcaatacact acatggcgtg atttcatatg cgcgattgct
     5521 gatccccatg tgtatcactg gcaaactgtg atggacgaca ccgtcagtgc gtccgtcgcg
     5581 caggctctcg atgagctgat gctttgggcc gaggactgcc ccgaagtccg gcacctcgtg
     5641 cacgcggatt tcggctccaa caatgtcctg acggacaatg gccgcataac agcggtcatt
     5701 gactggagcg aggcgatgtt cggggattcc caatacgagg tcgccaacat cttcttctgg
     5761 aggccgtggt tggcttgtat ggagcagcag acgcgctact tcgagcggag gcatccggag
     5821 cttgcaggat cgccgcggct ccgggcgtat atgctccgca ttggtcttga ccaactctat
     5881 cagagcttgg ttgacggcaa tttcgatgat gcagcttggg cgcagggtcg atgcgacgca
     5941 atcgtccgat ccggagccgg gactgtcggg cgtacacaaa tcgcccgcag aagcgcggcc
     6001 gtctggaccg atggctgtgt agaagtactc gccgatagtg gaaaccgacg ccccagcact
     6061 cgtccgaggg caaaggaata gcacgtacta cgagatttcg attccaccgc cgccttctat
     6121 gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg
     6181 gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac
     6241 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     6301 tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc
     6361 tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
     6421 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
     6481 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
     6541 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
     6601 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     6661 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     6721 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     6781 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     6841 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     6901 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     6961 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     7021 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     7081 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     7141 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     7201 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     7261 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     7321 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     7381 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     7441 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     7501 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     7561 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     7621 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     7681 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     7741 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     7801 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     7861 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     7921 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     7981 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     8041 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     8101 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     8161 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     8221 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     8281 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     8341 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     8401 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     8461 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     8521 aaagtgccac ctgacgtcga cggatcggga gatctcccga tcccctatgg tgcactctca
     8581 gtacaatctg ctctgatgcc gcatagttaa gccagtatct gctccctgct tgtgtgttgg
     8641 aggtcgctga gtagtgcgcg agcaaaattt aagctacaac aaggcaaggc ttgaccgaca
     8701 attgcatgaa gaatctgctt agggttaggc gttttgcgct gcttcgcgat gt
//
