LOCUS       sequence-376734-       10699 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For use in RBP tethering screen.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10699)
  AUTHORS   Luo EC, Nathanson JL, Tan FE, Schwartz JL, Schmok JC, Shankar A, 
            Markmiller S, Yee BA, Sathe S, Pratt GA, Scaletta DB, Ha Y, Hill DE,
            Aigner S, Yeo GW
  TITLE     Large-scale tethered function assays identify factors that regulate 
            mRNA stability and translation.
  JOURNAL   Nat Struct Mol Biol. 2020 Oct;27(10):989-1000. doi: 
            10.1038/s41594-020-0477-6. Epub 2020 Aug 17.
  PUBMED    32807991
REFERENCE   2  (bases 1 to 10699)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41594-020-0477-6"; journalName: "Nat Struct Mol Biol"; 
            date: "2020-10"; volume: "27"; issue: "10"; pages: "989-1000"
COMMENT     Sequence Label: CPSF1
FEATURES             Location/Qualifiers
     source          1..10699
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     primer_bind     1196..1215
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1196..1214
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1293..1317
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5656..5680)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5805..5846
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(6301..6318)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6307..6531
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6664..6683)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    6815..6862
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             6870..7892
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             6870..7892
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    8022..8143
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8059..8078)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8113..8132
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8192..8208)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8192..8208)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8205..8227)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8216..8232
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8240..8246,8247..8264,8265..8270))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8285..8306
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8423..8440)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8594..9182)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8674..8693)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9353..10144,10145..10213))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9976..9995
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10214..10318)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10393..10412)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gaattagctt ggtactaata
     1201 cgactcacta tagggagacc caagctggct aggtaagctt ggtaccgagc tcggatccac
     1261 tagtccagtg tggtggaatt ctgcagatat caacaagttt gtacaaaaaa gcaggctcca
     1321 ccatgtacgc cgtgtacaaa caggcgcatc cgcccaccgg tctggagttc tccatgtact
     1381 gcaacttctt caacaacagc gagcgcaacc tggtagtggc cgggacctcg cagctctacg
     1441 tgtaccgcct caaccgcgac gccgaggctc tgaccaagaa tgacaggagc acagagggga
     1501 aggcccaccg ggagaagctc gagcttgctg cctccttctc cttctttggc aacgtcatgt
     1561 ccatggccag cgtgcagctg gcaggagcca agcgggatgc cctgctccta agcttcaagg
     1621 atgccaagct gtctgtggtg gagtacgacc cgggcaccca tgacctgaag accctgtcac
     1681 tgcactactt tgaggagcct gagcttcggg acgggtttgt gcagaatgta cacacgccgc
     1741 gagtgcgggt ggaccccgac gggcgctgtg cagccatgct tgtctacggc acgcggctgg
     1801 tggtcctgcc cttccgcagg gagagcctgg ctgaggagca cgaggggctc gtgggtgagg
     1861 ggcagaggtc cagcttcctg cccagctaca tcatcgacgt gcgggcccta gacgagaagc
     1921 tgctcaacat catcgacctg cagttcctgc atggctacta cgagcctacc ctcctcatcc
     1981 tgtttgagcc caaccagacc tggcctgggc gcgtggccgt gcggcaggac acgtgctcca
     2041 ttgtggccat ctcactgaac atcacgcaga aggtgcaccc cgtcatctgg tccctcacca
     2101 gcctgccctt tgactgcacc caggctctgg ctgtgcccaa gcccataggt ggggtggtgg
     2161 tgtttgccgt caactcgctg ttgtacctga accagagcgt ccccccgtat ggcgtggctc
     2221 tcaacagcct caccacagga accacggctt tcccgcttcg cacccaggag ggtgtgcgga
     2281 tcaccctgga ctgcgcccag gccaccttca tctcctacga caagatggtc atctccctca
     2341 agggcggcga gatctacgtg ctgaccctca tcaccgacgg catgcgcagt gtccgagcgt
     2401 tccactttga caaggcggcc gccagcgtcc tcaccaccag catggtcacc atggagcccg
     2461 ggtacctgtt cctgggttct cgcctgggca attccctcct cctcaagtac acggagaagc
     2521 tgcaggagcc cccggccagt gctgtccgtg aggctgccga caaggaagag cctccctcaa
     2581 agaagaagcg agtggatgcg acggccggct ggtcagctgc gggtaagtcg gtgccgcagg
     2641 atgaggtgga cgagattgaa gtgtacggca gcgaggccca gtcgggaaca cagctggcca
     2701 cctactcctt tgaggtgtgt gacagcatcc tgaacattgg accctgtgcc aatgccgccg
     2761 tgggcgagcc tgccttcctc tctgaagagt ttcagaacag ccccgagccg gacctggaga
     2821 ttgtggtttg ctccggccac gggaagaacg gggctttgtc ggtgctgcag aagagcatcc
     2881 ggccccaggt ggtgacaacc tttgagcttc ccggctgcta tgacatgtgg acagtcatcg
     2941 ccccggtgcg taaggaggag gaggacaatc ccaaggggga gggcacagag caggaaccca
     3001 gcaccacccc tgaagcagac gacgacggcc gcagacacgg attcctgatt ctgagccggg
     3061 aagactccac catgatcctg cagacggggc aggagatcat ggagctggac accagtggct
     3121 tcgccactca gggccccacg gtctttgctg ggaacatcgg ggacaaccgc tacattgtcc
     3181 aagtgtcacc actgggcatc cgcctgctgg aaggagtgaa tcagctgcac ttcatccccg
     3241 tggacctggg cgcccccatc gtgcagtgcg ccgtggccga cccctatgtg gtcatcatga
     3301 gtgccgaggg ccacgtcacc atgttcctgc tgaagagtga ctcctacggt ggccgccacc
     3361 accgcctggc gctgcacaag cccccgctgc accatcagtc caaggtgatt acgctgtgcc
     3421 tgtaccgaga cctcagcggc atgttcacca ctgagagccg cctgggtggg gcccgtgacg
     3481 agctcggggg ccgcagtggc ccggaggccg agggcctggg ctcagagact agccccacag
     3541 tggatgacga ggaggagatg ctgtatgggg attcgggctc cctcttcagc cccagcaagg
     3601 aggaggcccg aagaagcagc cagccccctg ctgaccggga ccctgcaccc ttccgggcag
     3661 agcctaccca ctggtgcctg ctggtgcggg agaatggcac catggagatc taccagcttc
     3721 ccgactggcg gctggtgttc ctggtgaaga acttccctgt ggggcagcgg gtccttgtgg
     3781 acagctcctt tggacagccc actacacagg gcgaggcccg cagggaggag gccacgcgcc
     3841 agggggagct gcccctcgtc aaggaggtgc tgctggtggc gctgggcagc cgccagagca
     3901 ggccctacct gctggtgcat gtggaccaag agctgcttat ctacgaggcc ttcccccacg
     3961 actctcagct cggccagggc aatctcaaag tccgctttaa gaaggtccct cacaacatca
     4021 acttccgtga gaagaagcca aagccatcca agaagaaagc agaaggtggc ggcgcagagg
     4081 agggggctgg ggcccggggc cgcgtggcgc gtttccgcta cttcgaggat atttatggct
     4141 actcaggggt cttcatctgc ggcccctccc ctcactggct cttggtgacc ggccgagggg
     4201 ctctgcggct acaccccatg gccatcgacg gcccggtcga ctctttcgct ccattccaca
     4261 atgtcaactg tccccgcggc ttcctgtact tcaacagaca gggcgagctg aggatcagtg
     4321 tcctgcctgc ctacctgtcc tatgatgccc catggcctgt caggaagatc ccgctgcgct
     4381 gcacggccca ctatgtggct taccacgtgg agtctaaggt gtatgctgtg gccaccagca
     4441 ccaacacgcc gtgtgcccgc atcccacgca tgactggcga ggagaaggag tttgagacca
     4501 tcgagagaga tgagcggtac atccaccccc agcaggaggc cttctccatc cagctcatct
     4561 ccccggtcag ctgggaggct attcccaatg ccaggatcga gctgcaggag tgggagcatg
     4621 tgacctgcat gaagacagtg tctctgcgca gtgaggagac cgtgtcgggc ctcaaaggct
     4681 acgtggccgc cgggacctgc ctcatgcagg gggaggaggt cacgtgccga gggcggatct
     4741 tgatcatgga tgtgattgag gtggtgcccg agcctggcca gcccttgacc aagaacaagt
     4801 tcaaagtcct ttacgagaag gagcagaagg ggcccgtgac cgccctgtgc cactgcaatg
     4861 gccacctggt gtcggccatc ggccagaaga ttttcctgtg gagcctgcgg gccagcgagc
     4921 tgacgggcat ggccttcatc gacacgcagc tctacataca ccagatgatc agcgtcaaga
     4981 acttcatcct ggcagccgac gtcatgaaga gcatttcgct gctgcgctac caggaggaaa
     5041 gcaagacgct gagcctggtg tcgcgggatg ccaagcccct ggaggtgtac agcgtggact
     5101 tcatggtgga caatgcccag ctgggttttc tggtgtctga ccgcgaccgc aacctcatgg
     5161 tgtacatgta cctgcccgaa gccaaggaga gtttcggggg catgcgcctg ctgcgtcggg
     5221 cagacttcca cgtgggtgcc cacgtgaaca cgttctggag gaccccgtgc cggggggcca
     5281 ctgaagggct cagcaaaaag tcggtcgtgt gggagaataa gcacatcacg tggtttgcca
     5341 ccctggacgg cggcatcggg ctgctgctgc ccatgcagga gaagacctac cggcggctgc
     5401 tgatgctgca gaacgcgctg accaccatgc tgccacacca cgccggcctc aacccccgcg
     5461 ccttccggat gctgcacgtg gaccgccgca ccctccagaa tgccgtgcgc aacgtgctgg
     5521 atggggagct gctcaaccgc tacctgtacc tgagcaccat ggagcgcagc gagctagcca
     5581 agaagatcgg caccacacca gacataatcc tggacgactt gctggagacg gaccgcgtca
     5641 ccgcccactt cttggaccca gctttcttgt acaaagtggt tgatatccag cacagtggcg
     5701 gccgctcgag tctagagggc ccgcggttcg aaaccagcgg aggcggcgga agcgggggag
     5761 gaggcagcgg cggaggcggc agcgggggcg gcggaagcgc taccggtaag cctatcccta
     5821 accctctcct cggtctcgat tctacgcgta ccggtatggc ttctaacttt actcagttcg
     5881 ttctcgtcga caatggcgga actggcgacg tgactgtcgc cccaagcaac ttcgctaacg
     5941 gggtcgctga atggatcagc tctaactcgc gatcacaggc ttacaaagta acctgtagcg
     6001 ttcgtcagag ctctgcgcag aatcgcaaat acaccatcaa agtcgaggtg cctaaagtgg
     6061 caacccagac tgttggtggt gaagagcttc ctgtagccgg atggagatct tacttaaata
     6121 tggaactaac cattccaatt ttcgccacga attccgactg cgagcttatt gttaaggcaa
     6181 tgcaaggtct cctaaaagat ggaaacccga ttccctcggc catcgcggcc aactccggca
     6241 tctacggagg tggaggtgga tctcgtacgg gttagtaatg agtttaaacc cgctgatcag
     6301 cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct
     6361 tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc
     6421 attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg
     6481 aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg
     6541 cggaaagaac cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa
     6601 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
     6661 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     6721 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     6781 aaaaacttga ttagggtgat ggttcacgta cctagaagtt cctattccga agttcctatt
     6841 ctctagaaag tataggaact tccttggcca aaaagcctga actcaccgcg acgtctgtcg
     6901 agaagtttct gatcgaaaag ttcgacagcg tctccgacct gatgcagctc tcggagggcg
     6961 aagaatctcg tgctttcagc ttcgatgtag gagggcgtgg atatgtcctg cgggtaaata
     7021 gctgcgccga tggtttctac aaagatcgtt atgtttatcg gcactttgca tcggccgcgc
     7081 tcccgattcc ggaagtgctt gacattgggg aattcagcga gagcctgacc tattgcatct
     7141 cccgccgtgc acagggtgtc acgttgcaag acctgcctga aaccgaactg cccgctgttc
     7201 tgcagccggt cgcggaggcc atggatgcga tcgctgcggc cgatcttagc cagacgagcg
     7261 ggttcggccc attcggaccg caaggaatcg gtcaatacac tacatggcgt gatttcatat
     7321 gcgcgattgc tgatccccat gtgtatcact ggcaaactgt gatggacgac accgtcagtg
     7381 cgtccgtcgc gcaggctctc gatgagctga tgctttgggc cgaggactgc cccgaagtcc
     7441 ggcacctcgt gcacgcggat ttcggctcca acaatgtcct gacggacaat ggccgcataa
     7501 cagcggtcat tgactggagc gaggcgatgt tcggggattc ccaatacgag gtcgccaaca
     7561 tcttcttctg gaggccgtgg ttggcttgta tggagcagca gacgcgctac ttcgagcgga
     7621 ggcatccgga gcttgcagga tcgccgcggc tccgggcgta tatgctccgc attggtcttg
     7681 accaactcta tcagagcttg gttgacggca atttcgatga tgcagcttgg gcgcagggtc
     7741 gatgcgacgc aatcgtccga tccggagccg ggactgtcgg gcgtacacaa atcgcccgca
     7801 gaagcgcggc cgtctggacc gatggctgtg tagaagtact cgccgatagt ggaaaccgac
     7861 gccccagcac tcgtccgagg gcaaaggaat agcacgtact acgagatttc gattccaccg
     7921 ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc
     7981 tccagcgcgg ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt
     8041 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     8101 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt
     8161 cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     8221 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     8281 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     8341 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     8401 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     8461 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     8521 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     8581 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     8641 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     8701 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     8761 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     8821 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     8881 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     8941 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     9001 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     9061 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     9121 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     9181 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     9241 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     9301 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     9361 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     9421 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     9481 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     9541 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     9601 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     9661 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     9721 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     9781 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     9841 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     9901 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     9961 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
    10021 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
    10081 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
    10141 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
    10201 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
    10261 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
    10321 catttccccg aaaagtgcca cctgacgtcg acggatcggg agatctcccg atcccctatg
    10381 gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtatc tgctccctgc
    10441 ttgtgtgttg gaggtcgctg agtagtgcgc gagcaaaatt taagctacaa caaggcaagg
    10501 cttgaccgac aattgcatga agaatctgct tagggttagg cgttttgcgc tgcttcgcga
    10561 tgtacgggcc agatatacgc gttgacattg attattgact aggcttttgc aaaaagcttt
    10621 gcaaagatgg ataaagtttt aaacagagag gaatctttgc agctaatgga ccttctaggt
    10681 cttgaaagga gtgggaatt
//
