LOCUS       sequence-376739-        9028 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For use in RBP tethering screen.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9028)
  AUTHORS   Luo EC, Nathanson JL, Tan FE, Schwartz JL, Schmok JC, Shankar A, 
            Markmiller S, Yee BA, Sathe S, Pratt GA, Scaletta DB, Ha Y, Hill DE,
            Aigner S, Yeo GW
  TITLE     Large-scale tethered function assays identify factors that regulate 
            mRNA stability and translation.
  JOURNAL   Nat Struct Mol Biol. 2020 Oct;27(10):989-1000. doi: 
            10.1038/s41594-020-0477-6. Epub 2020 Aug 17.
  PUBMED    32807991
REFERENCE   2  (bases 1 to 9028)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41594-020-0477-6"; journalName: "Nat Struct Mol Biol"; 
            date: "2020-10"; volume: "27"; issue: "10"; pages: "989-1000"
COMMENT     Sequence Label: EXOSC10
FEATURES             Location/Qualifiers
     source          1..9028
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     primer_bind     1196..1215
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1196..1214
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1293..1317
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3985..4009)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4134..4175
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(4630..4647)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4636..4860
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(4993..5012)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    5144..5191
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             5199..6221
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             5199..6221
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    6351..6472
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6388..6407)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6442..6461
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6521..6537)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6521..6537)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6534..6556)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6545..6561
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6569..6575,6576..6593,6594..6599))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6614..6635
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6752..6769)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6923..7511)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7003..7022)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7682..8473,8474..8542))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8305..8324
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8543..8647)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8722..8741)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gaattagctt ggtactaata
     1201 cgactcacta tagggagacc caagctggct aggtaagctt ggtaccgagc tcggatccac
     1261 tagtccagtg tggtggaatt ctgcagatat caacaagttt gtacaaaaaa gcaggctcca
     1321 ccatggcgcc acccagtacc cgggagccca gggtcctgtc ggcgaccagc gcaaccaaat
     1381 ccgacggaga gatggtgctg ccaggcttcc cggacgccga cagctttgtg aagtttgctc
     1441 ttgggtccgt ggtggcagtc accaaggcat ctgggggcct accacagttt ggcgatgagt
     1501 atgattttta ccgaagtttt cctggcttcc aagcattttg cgaaacacag ggagacaggt
     1561 tgcttcagtg catgagcaga gtaatgcagt accatgggtg tcgcagcaac attaaggatc
     1621 gaagtaaagt gactgagctg gaagacaagt ttgatttact agttgatgcc aatgatgtaa
     1681 ttctggagag agtgggtatt ttactggatg aagcctcagg tgtaaacaag aatcaacagc
     1741 ctgtcctccc tgccggcttg caggtcccca aaacggtagt gtccagctgg aaccgtaagg
     1801 cagcagaata tggcaaaaaa gcaaaatctg aaactttccg gctgcttcat gcaaaaaata
     1861 tcatccgacc tcagctcaag tttcgagaga agattgacaa ttccaacaca ccatttcttc
     1921 ctaaaatctt catcaaaccc aatgctcaga aacctctccc tcaagctctc tctaaggaaa
     1981 ggcgggaacg cccacaggat cgtcctgagg acttggacgt cccccctgca ctggctgatt
     2041 tcatccatca gcagagaacc cagcaggttg agcaagacat gtttgcacat ccttatcaat
     2101 atgaactaaa tcactttacc ccagcagatg cagtgcttca aaagccacaa ccccagttat
     2161 acagacctat agaagagaca ccatgccatt tcatatcctc cctggatgaa ctcgtggaac
     2221 tcaacgaaaa gctcttgaat tgtcaggaat ttgcagttga cttggagcac cactcttaca
     2281 ggagcttcct gggactgacc tgcctgatgc aaatttctac tcggacggaa gacttcatca
     2341 ttgacaccct cgagcttcga agtgacatgt acattctcaa tgagagcctc acagacccag
     2401 ccatcgttaa ggtctttcat ggtgctgatt cagacataga atggctacag aaagactttg
     2461 ggttgtatgt agtaaacatg tttgatactc atcaggcagc acgccttctt aacctgggca
     2521 ggcactcact cgatcatctc ctgaaactct actgcaacgt ggactcaaac aagcaatatc
     2581 agctggctga ttggagaata cgccctctgc ccgaggagat gctcagctac gcccgggatg
     2641 acacccatta cctgctatat atctatgaca aaatgaggct ggagatgtgg gagcgcggca
     2701 acgggcagcc cgtgcagctg caggtggtgt ggcaacggag cagggacatc tgcctcaaga
     2761 aattcatcaa acctatcttc acggatgagt cctaccttga actctatagg aagcagaaga
     2821 agcaccttaa cacacagcag ttgacagcct ttcagctgct gtttgcctgg agggataaaa
     2881 cagctcgcag ggaagatgaa agttacggat atgtactgcc aaaccacatg atgctgaaaa
     2941 tagctgaaga actgcctaag gaacctcagg gcatcatagc ttgctgcaac ccagtaccgc
     3001 cccttgtgcg gcagcagatc aacgaaatgc accttttaat ccagcaggcc cgagagatgc
     3061 ccctgctcaa gtctgaagtt gcagccggag tgaagaagag cggaccgctg cccagtgctg
     3121 agagattgga gaatgttctc tttggacctc acgactgctc ccatgcccct ccggatggct
     3181 atccaatcat cccaaccagt ggatctgtgc cagttcagaa gcaggcgagc ctcttccctg
     3241 atgaaaaaga agataacttg ctgggtacca catgcctgat tgccacagct gtcatcacgt
     3301 tatttaatga acctagtgct gaagacagta aaaagggtcc attgacagtt gcacagaaaa
     3361 aagcccagaa catcatggag tcctttgaaa atccatttag gatgtttctg ccctcactgg
     3421 gacaccgtgc tcccgtctct caggcagcga agttcgatcc atcaaccaaa atctatgaaa
     3481 tcagcaaccg ttggaagctg gcccaggtac aagtacaaaa agactctaaa gaagctgtca
     3541 agaagaaggc agctgagcaa acagctgccc gggaacaggc aaaggaggcg tgcaaagctg
     3601 cagcagaaca ggccatctcc gtccgacagc aggtcgtgct agaaaatgct gcaaagaaga
     3661 gagagcgagc aacaagcgac ccaaggacca cagaacagaa acaagagaag aaacgactca
     3721 aaatttccaa gaagccaaag gacccagagc caccagaaaa agagtttacg ccttacgact
     3781 acagccagtc agacttcaag gcttttgctg gaaacagcaa atccaaagtt tcttctcagt
     3841 ttgatccaaa taaacagacc ccgtctggca agaaatgcat tgcagccaaa aaaattaaac
     3901 agtcggtggg aaacaaaagc atgtcctttc caactggaaa gtcagacaga ggcttcaggt
     3961 acaactggcc acagagatgg atccacccag ctttcttgta caaagtggtt gatatccagc
     4021 acagtggcgg ccgctcgagt ctagagggcc cgcggttcga aaccagcgga ggcggcggaa
     4081 gcgggggagg aggcagcggc ggaggcggca gcgggggcgg cggaagcgct accggtaagc
     4141 ctatccctaa ccctctcctc ggtctcgatt ctacgcgtac cggtatggct tctaacttta
     4201 ctcagttcgt tctcgtcgac aatggcggaa ctggcgacgt gactgtcgcc ccaagcaact
     4261 tcgctaacgg ggtcgctgaa tggatcagct ctaactcgcg atcacaggct tacaaagtaa
     4321 cctgtagcgt tcgtcagagc tctgcgcaga atcgcaaata caccatcaaa gtcgaggtgc
     4381 ctaaagtggc aacccagact gttggtggtg aagagcttcc tgtagccgga tggagatctt
     4441 acttaaatat ggaactaacc attccaattt tcgccacgaa ttccgactgc gagcttattg
     4501 ttaaggcaat gcaaggtctc ctaaaagatg gaaacccgat tccctcggcc atcgcggcca
     4561 actccggcat ctacggaggt ggaggtggat ctcgtacggg ttagtaatga gtttaaaccc
     4621 gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg
     4681 tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa
     4741 ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca
     4801 gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg
     4861 cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg
     4921 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     4981 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     5041 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
     5101 tcgaccccaa aaaacttgat tagggtgatg gttcacgtac ctagaagttc ctattccgaa
     5161 gttcctattc tctagaaagt ataggaactt ccttggccaa aaagcctgaa ctcaccgcga
     5221 cgtctgtcga gaagtttctg atcgaaaagt tcgacagcgt ctccgacctg atgcagctct
     5281 cggagggcga agaatctcgt gctttcagct tcgatgtagg agggcgtgga tatgtcctgc
     5341 gggtaaatag ctgcgccgat ggtttctaca aagatcgtta tgtttatcgg cactttgcat
     5401 cggccgcgct cccgattccg gaagtgcttg acattgggga attcagcgag agcctgacct
     5461 attgcatctc ccgccgtgca cagggtgtca cgttgcaaga cctgcctgaa accgaactgc
     5521 ccgctgttct gcagccggtc gcggaggcca tggatgcgat cgctgcggcc gatcttagcc
     5581 agacgagcgg gttcggccca ttcggaccgc aaggaatcgg tcaatacact acatggcgtg
     5641 atttcatatg cgcgattgct gatccccatg tgtatcactg gcaaactgtg atggacgaca
     5701 ccgtcagtgc gtccgtcgcg caggctctcg atgagctgat gctttgggcc gaggactgcc
     5761 ccgaagtccg gcacctcgtg cacgcggatt tcggctccaa caatgtcctg acggacaatg
     5821 gccgcataac agcggtcatt gactggagcg aggcgatgtt cggggattcc caatacgagg
     5881 tcgccaacat cttcttctgg aggccgtggt tggcttgtat ggagcagcag acgcgctact
     5941 tcgagcggag gcatccggag cttgcaggat cgccgcggct ccgggcgtat atgctccgca
     6001 ttggtcttga ccaactctat cagagcttgg ttgacggcaa tttcgatgat gcagcttggg
     6061 cgcagggtcg atgcgacgca atcgtccgat ccggagccgg gactgtcggg cgtacacaaa
     6121 tcgcccgcag aagcgcggcc gtctggaccg atggctgtgt agaagtactc gccgatagtg
     6181 gaaaccgacg ccccagcact cgtccgaggg caaaggaata gcacgtacta cgagatttcg
     6241 attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct
     6301 ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta
     6361 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     6421 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     6481 gtataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt
     6541 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
     6601 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
     6661 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
     6721 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     6781 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     6841 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     6901 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     6961 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     7021 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     7081 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     7141 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     7201 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     7261 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     7321 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     7381 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     7441 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     7501 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     7561 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     7621 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     7681 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     7741 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     7801 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     7861 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     7921 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     7981 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     8041 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     8101 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     8161 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     8221 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     8281 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     8341 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     8401 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     8461 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     8521 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     8581 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     8641 ttccgcgcac atttccccga aaagtgccac ctgacgtcga cggatcggga gatctcccga
     8701 tcccctatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatct
     8761 gctccctgct tgtgtgttgg aggtcgctga gtagtgcgcg agcaaaattt aagctacaac
     8821 aaggcaaggc ttgaccgaca attgcatgaa gaatctgctt agggttaggc gttttgcgct
     8881 gcttcgcgat gtacgggcca gatatacgcg ttgacattga ttattgacta ggcttttgca
     8941 aaaagctttg caaagatgga taaagtttta aacagagagg aatctttgca gctaatggac
     9001 cttctaggtc ttgaaaggag tgggaatt
//
