LOCUS       sequence-376749-        8698 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For use in RBP tethering screen.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8698)
  AUTHORS   Luo EC, Nathanson JL, Tan FE, Schwartz JL, Schmok JC, Shankar A, 
            Markmiller S, Yee BA, Sathe S, Pratt GA, Scaletta DB, Ha Y, Hill DE,
            Aigner S, Yeo GW
  TITLE     Large-scale tethered function assays identify factors that regulate 
            mRNA stability and translation.
  JOURNAL   Nat Struct Mol Biol. 2020 Oct;27(10):989-1000. doi: 
            10.1038/s41594-020-0477-6. Epub 2020 Aug 17.
  PUBMED    32807991
REFERENCE   2  (bases 1 to 8698)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41594-020-0477-6"; journalName: "Nat Struct Mol Biol"; 
            date: "2020-10"; volume: "27"; issue: "10"; pages: "989-1000"
COMMENT     Sequence Label: DHX40
FEATURES             Location/Qualifiers
     source          1..8698
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        114..1292
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          345..1283
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1240..1260
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     primer_bind     1309..1328
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1309..1327
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1406..1430
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3768..3792)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3917..3958
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(4413..4430)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4419..4643
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(4776..4795)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    4927..4974
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             4982..6004
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             4982..6004
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    6134..6255
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6171..6190)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6225..6244
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6304..6320)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6304..6320)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6317..6339)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6328..6344
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6352..6358,6359..6376,6377..6382))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6397..6418
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6535..6552)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6706..7294)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6786..6805)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7465..8256,8257..8325))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8088..8107
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8326..8430)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8505..8524)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 attgattatt gactaggctt ttgcaaaaag ctttgcaaag atggataaag ttttaaacag
       61 agaggaatct ttgcagctaa tggaccttct aggtcttgaa aggagtggga attggctccg
      121 gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc cgagaagttg gggggagggg
      181 tcggcaattg aaccggtgcc tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg
      241 tgtactggct ccgccttttt cccgagggtg ggggagaacc gtatataagt gcagtagtcg
      301 ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac acaggtaagt gccgtgtgtg
      361 gttcccgcgg gcctggcctc tttacgggtt atggcccttg cgtgccttga attacttcca
      421 cctggctgca gtacgtgatt cttgatcccg agcttcgggt tggaagtggg tgggagagtt
      481 cgaggccttg cgcttaagga gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc
      541 gctggggccg ccgcgtgcga atctggtggc accttcgcgc ctgtctcgct gctttcgata
      601 agtctctagc catttaaaat ttttgatgac ctgctgcgac gctttttttc tggcaagata
      661 gtcttgtaaa tgcgggccaa gatctgcaca ctggtatttc ggtttttggg gccgcgggcg
      721 gcgacggggc ccgtgcgtcc cagcgcacat gttcggcgag gcggggcctg cgagcgcggc
      781 caccgagaat cggacggggg tagtctcaag ctggccggcc tgctctggtg cctggcctcg
      841 cgccgccgtg tatcgccccg ccctgggcgg caaggctggc ccggtcggca ccagttgcgt
      901 gagcggaaag atggccgctt cccggccctg ctgcagggag ctcaaaatgg aggacgcggc
      961 gctcgggaga gcgggcgggt gagtcaccca cacaaaggaa aagggccttt ccgtcctcag
     1021 ccgtcgcttc atgtgactcc acggagtacc gggcgccgtc caggcacctc gattagttct
     1081 cgagcttttg gagtacgtcg tctttaggtt ggggggaggg gttttatgcg atggagtttc
     1141 cccacactga gtgggtggag actgaagtta ggccagcttg gcacttgatg taattctcct
     1201 tggaatttgc cctttttgag tttggatctt ggttcattct caagcctcag acagtggttc
     1261 aaagtttttt tcttccattt caggtgtcgt gaggaattag cttggtacta atacgactca
     1321 ctatagggag acccaagctg gctaggtaag cttggtaccg agctcggatc cactagtcca
     1381 gtgtggtgga attctgcaga tatcaacaag tttgtacaaa aaagttggca tgtcccggtt
     1441 tcccgcagtc gcgggcaggg cgccaaggcg gcaggaggag ggtgagcggt caagagacct
     1501 ccaggaagag cggctctcgg ctgtttgcat cgccgataga gaagagaaag gatgcacgtc
     1561 ccaggaggga ggaactactc caacttttcc tattcagaaa caaagaaaaa agattattca
     1621 agctgtgagg gacaattcat tccttattgt tactggaaat acaggaagtg gtaaaacaac
     1681 tcaactccca aaatatctat atgaagcagg gttttcacaa catggtatga ttggtgtaac
     1741 tcaaccacga aaagtagctg ctatatcagt tgctcagaga gtagctgaag aaatgaaatg
     1801 cactttggga tccaaagtag gataccaagt tcgttttgat gattgcagtt ctaaggagac
     1861 agcaatcaaa tatatgactg atggatgttt actgaaacat attctgggag acccaaatct
     1921 taccaaattc agtgtcatta ttttggatga agcccatgaa agaactctaa ctacagatat
     1981 cttatttggt ttattgaaga agctatttca ggagaagtct cctaatagga aggagcattt
     2041 aaaagtggtg gtaatgtcag caactatgga attagccaag ctctctgcat tctttggaaa
     2101 ttgtccaata tttgatatac ctggaaggct ttatccagtc agagagaaat tctgcaattt
     2161 gattggtcca cgagacagag aaaatactgc gtatattcaa gcgattgtga aagtcaccat
     2221 ggatatccat ttgaatgaaa tggctggaga catcttggtt tttctgactg gccagtttga
     2281 aatagaaaaa agttgtgagt tactttttca gatggcagag tctgttgatt atgattatga
     2341 tgttcaagat accaccctcg atggcttgtt aatattgccg tgttatggat caatgacaac
     2401 agatcaacag aggaggatat ttttgccacc accacctgga attagaaaat gtgtcatatc
     2461 caccaatatt tctgcaacgt ctttgacaat agatggaatc agatatgtgg tagatggtgg
     2521 cttcgtgaag cagttaaatc acaaccccag attagggttg gacatcctgg aggtggttcc
     2581 aatttcaaag agcgaggcat tacagcgaag tggccgagct ggcaggactt cttcaggaaa
     2641 atgctttcgg atctatagta aagatttttg gaaccagtgt atgcctgacc atgtgatccc
     2701 tgaaattaag agaactagtt tgacatctgt agttctgacc ttaaagtgcc ttgccataca
     2761 cgatgtcata aggtttccct atttggatcc acctaatgag agacttattt tagaagctct
     2821 taaacaactt taccagtgtg atgctattga caggagtggc catgtcacca gattgggttt
     2881 gtctatggtg gagtttcctt tgcctccaca tctgacatgt gcagtaataa aagctgcttc
     2941 cctggattgt gaagatctac tacttccaat agcagcaatg ttgtctgtgg aaaacgtctt
     3001 cattagacct gttgatccag agtaccagaa ggaagcagaa cagagacatc gagaattggc
     3061 agctaaagct ggaggattta atgactttgc aactttagct gtcatctttg aacaatgcaa
     3121 atcaagtgga gctccagctt catggtgcca aaaacactgg attcattgga ggtgcttatt
     3181 ttctgcattt cgtgtggaag ctcaacttcg agaactaatc aggaagctta aacagcaaag
     3241 tgatttccca aaagagacct ttgaaggccc taaacatgaa gtactacgaa gatgtctttg
     3301 tgcgggctat ttcaaaaatg tagctcgaag atctgttggg agaacgtttt gcacaatgga
     3361 tggtcgtgga agcccagttc acattcatcc ttcctcagca cttcatgaac aggaaaccaa
     3421 acttgaatgg atcatttttc atgaggtatt ggttaccacc aaagtctacg caagaattgt
     3481 atgcccaatc cgttatgaat gggtaagaga cttgttaccc aagttgcatg aatttaatgc
     3541 acatgatttg agcagtgtgg cccgacgtga agtgagagaa gatgcaagaa ggagatggac
     3601 aaataaggaa aatgtaaagc agctaaagga tggaatatcg aaagacgtct taaagaaaat
     3661 gcaaagaaga aatgatgaca aatccatatc tgatgcacgg gctcgtttcc ttgagagaaa
     3721 gcagcagagg acccaggacc acagtgacac acgaaaggaa acaggctacc caactttctt
     3781 gtacaaagtg gttgatatcc agcacagtgg cggccgctcg agtctagagg gcccgcggtt
     3841 cgaaaccagc ggaggcggcg gaagcggggg aggaggcagc ggcggaggcg gcagcggggg
     3901 cggcggaagc gctaccggta agcctatccc taaccctctc ctcggtctcg attctacgcg
     3961 taccggtatg gcttctaact ttactcagtt cgttctcgtc gacaatggcg gaactggcga
     4021 cgtgactgtc gccccaagca acttcgctaa cggggtcgct gaatggatca gctctaactc
     4081 gcgatcacag gcttacaaag taacctgtag cgttcgtcag agctctgcgc agaatcgcaa
     4141 atacaccatc aaagtcgagg tgcctaaagt ggcaacccag actgttggtg gtgaagagct
     4201 tcctgtagcc ggatggagat cttacttaaa tatggaacta accattccaa ttttcgccac
     4261 gaattccgac tgcgagctta ttgttaaggc aatgcaaggt ctcctaaaag atggaaaccc
     4321 gattccctcg gccatcgcgg ccaactccgg catctacgga ggtggaggtg gatctcgtac
     4381 gggttagtaa tgagtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc
     4441 atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt
     4501 cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct
     4561 ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc
     4621 tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg
     4681 gtatccccac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
     4741 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
     4801 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
     4861 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
     4921 tacctagaag ttcctattcc gaagttccta ttctctagaa agtataggaa cttccttggc
     4981 caaaaagcct gaactcaccg cgacgtctgt cgagaagttt ctgatcgaaa agttcgacag
     5041 cgtctccgac ctgatgcagc tctcggaggg cgaagaatct cgtgctttca gcttcgatgt
     5101 aggagggcgt ggatatgtcc tgcgggtaaa tagctgcgcc gatggtttct acaaagatcg
     5161 ttatgtttat cggcactttg catcggccgc gctcccgatt ccggaagtgc ttgacattgg
     5221 ggaattcagc gagagcctga cctattgcat ctcccgccgt gcacagggtg tcacgttgca
     5281 agacctgcct gaaaccgaac tgcccgctgt tctgcagccg gtcgcggagg ccatggatgc
     5341 gatcgctgcg gccgatctta gccagacgag cgggttcggc ccattcggac cgcaaggaat
     5401 cggtcaatac actacatggc gtgatttcat atgcgcgatt gctgatcccc atgtgtatca
     5461 ctggcaaact gtgatggacg acaccgtcag tgcgtccgtc gcgcaggctc tcgatgagct
     5521 gatgctttgg gccgaggact gccccgaagt ccggcacctc gtgcacgcgg atttcggctc
     5581 caacaatgtc ctgacggaca atggccgcat aacagcggtc attgactgga gcgaggcgat
     5641 gttcggggat tcccaatacg aggtcgccaa catcttcttc tggaggccgt ggttggcttg
     5701 tatggagcag cagacgcgct acttcgagcg gaggcatccg gagcttgcag gatcgccgcg
     5761 gctccgggcg tatatgctcc gcattggtct tgaccaactc tatcagagct tggttgacgg
     5821 caatttcgat gatgcagctt gggcgcaggg tcgatgcgac gcaatcgtcc gatccggagc
     5881 cgggactgtc gggcgtacac aaatcgcccg cagaagcgcg gccgtctgga ccgatggctg
     5941 tgtagaagta ctcgccgata gtggaaaccg acgccccagc actcgtccga gggcaaagga
     6001 atagcacgta ctacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg
     6061 aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt
     6121 cttcgcccac cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     6181 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     6241 catcaatgta tcttatcatg tctgtatacc gtcgacctct agctagagct tggcgtaatc
     6301 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
     6361 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
     6421 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
     6481 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     6541 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     6601 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     6661 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     6721 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     6781 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     6841 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     6901 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     6961 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     7021 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     7081 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     7141 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     7201 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     7261 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     7321 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     7381 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     7441 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     7501 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     7561 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     7621 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     7681 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     7741 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     7801 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     7861 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     7921 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     7981 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     8041 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     8101 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     8161 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     8221 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     8281 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     8341 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     8401 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
     8461 cgacggatcg ggagatctcc cgatccccta tggtgcactc tcagtacaat ctgctctgat
     8521 gccgcatagt taagccagta tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc
     8581 gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg acaattgcat gaagaatctg
     8641 cttagggtta ggcgttttgc gctgcttcgc gatgtacggg ccagatatac gcgttgac
//
