LOCUS       sequence-376752-        4383 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4383)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 4383)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLC5A8_STOP
FEATURES             Location/Qualifiers
     source          1..4383
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1940..2039)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2056..2075)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2057..2075)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2080..2096)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2080..2096)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2198..2217)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2209..3018
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2276..2295)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3165..3753
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3654..3673
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3907..3924
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4083..4110)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4202..4288)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4337..4359
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4351..4368
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4352..4368
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacacat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccaccatgga cacacctagg
      121 ggcatcggca ccttcgtggt gtgggattac gtggtgtttg ccggcatgct ggtcatctct
      181 gccgccatcg ggatctacta cgcattcgct ggtggaggac agcagaccag caaggacttt
      241 ctgatgggcg gccggagaat gacagcagtg cctgtggccc tgagcctgac cgcatctttc
      301 atgagcgccg tgacagtgct gggaacccca tccgaggtgt accgcttcgg cgccatcttt
      361 tctatcttcg cctttaccta tttctttgtg gtggtcatca gcgccgaggt gttcctgccc
      421 gtgttttaca agctgggcat cacatccacc tacgagtatc tggagctgcg gttcaataag
      481 tgcgtgagac tgtgcggcac cgtgctgttt atcgtgcaga caatcctgta caccggcatc
      541 gtgatctatg cccctgccct ggccctgaac caggtgacag gcttcgacct gtggggagca
      601 gtggtggcaa ccggcgtggt gtgcaccttc tactgtaccc tgggcggcct gaaggccgtg
      661 atctggacag acgtgttcca agtgggcatc atggtggccg gctttgcctc tgtgatcatc
      721 caggccgtgg tcatgcaggg cggcatcagc accatcctga atgacgccta tgatggcggc
      781 aggctgaact tctggaactt taatccaaac cccctgcagc gccacacctt ctggacaatc
      841 atcatcggcg gcaccttcac ctggacaagc atctacggcg tgaatcagtc ccaggtgcag
      901 aggtatatct cctgcaagtc tcgcttccag gccaagctga gcctgtacat caacctggtg
      961 ggcctgtggg ccatcctgac atgcagcgtg ttctgcggcc tggccctgta cagccggtat
     1021 cacgactgcg atccatggac cgccaagaag gtgtccgccc cagaccagct gatgccttac
     1081 ctggtgctgg acatcctgca ggattatcct ggactgccag gactgttcgt ggcatgtgca
     1141 tattctggca ccctgagcac agtgagctcc tctatcaatg cactggcagc agtgaccgtg
     1201 gaggatctga tcaagcccta ctttaggagc ctgtccgagc ggagcctgag ctggatctcc
     1261 cagggcatgt ctgtggtgta tggcgccctg tgcatcggaa tggccgccct ggcaagcctg
     1321 atgggcgccc tgctgcaggc cgccctgtcc gtgttcggaa tggtgggagg accactgatg
     1381 ggactgttcg cactgggaat cctggtgcct tttgccaatt ccatcggcgc cctggtggga
     1441 ctgatggcag gctttgccat ctctctgtgg gtgggaatcg gagcacagat ctacccacct
     1501 ctgccagagc ggacactgcc actgcacctg gacatccagg gctgtaactc cacatataat
     1561 gagaccaacc tgatgaccac aaccgagatg cccttcacca ccagcgtgtt ccagatctac
     1621 aatgtgcaga gaacccccct gatggacaac tggtattccc tgtcttacct gtatttcagc
     1681 accgtgggca cactggtgac cctgctggtg ggcatcctgg tgagcctgtc cacaggcggc
     1741 cggaagcaga atctggatcc cagatacatc ctgaccaagg aggacttcct gtccaacttc
     1801 gatatcttca agaagaagaa gcacgtgctg agctataagt cccaccctgt ggaggacggc
     1861 ggcacagata atccagcctt taaccacatc gagctgaatt ctgatcagag cggcaagtcc
     1921 aacggcacca ggctgtaaga cccagctttc ttgtacaaag ttggcattat aagaaagcat
     1981 tgcttatcaa tttgttgcaa cgaacaggtc actatcagtc aaaataaaat cattatttgc
     2041 catccagctg atatccccta tagtgagtcg tattacatgg tcatagctgt ttcctggcag
     2101 ctctggcccg tgtctcaaaa tctctgatgt tacattgcac aagataaaaa tatatcatca
     2161 tgaacaataa aactgtctgc ttacataaac agtaatacaa ggggtgttat gagccatatt
     2221 caacgggaaa cgtcgaggcc gcgattaaat tccaacatgg atgctgattt atatgggtat
     2281 aaatgggctc gcgataatgt cgggcaatca ggtgcgacaa tctatcgctt gtatgggaag
     2341 cccgatgcgc cagagttgtt tctgaaacat ggcaaaggta gcgttgccaa tgatgttaca
     2401 gatgagatgg tcagactaaa ctggctgacg gaatttatgc ctcttccgac catcaagcat
     2461 tttatccgta ctcctgatga tgcatggtta ctcaccactg cgatccccgg aaaaacagca
     2521 ttccaggtat tagaagaata tcctgattca ggtgaaaata ttgttgatgc gctggcagtg
     2581 ttcctgcgcc ggttgcattc gattcctgtt tgtaattgtc cttttaacag cgatcgcgta
     2641 tttcgtctcg ctcaggcgca atcacgaatg aataacggtt tggttgatgc gagtgatttt
     2701 gatgacgagc gtaatggctg gcctgttgaa caagtctgga aagaaatgca taaacttttg
     2761 ccattctcac cggattcagt cgtcactcat ggtgatttct cacttgataa ccttattttt
     2821 gacgagggga aattaatagg ttgtattgat gttggacgag tcggaatcgc agaccgatac
     2881 caggatcttg ccatcctatg gaactgcctc ggtgagtttt ctccttcatt acagaaacgg
     2941 ctttttcaaa aatatggtat tgataatcct gatatgaata aattgcagtt tcatttgatg
     3001 ctcgatgagt ttttctaatc agaattggtt aattggttgt aacactggca gagcattacg
     3061 ctgacttgac gggacggcgc aagctcatga ccaaaatccc ttaacgtgag ttacgcgtcg
     3121 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     3181 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     3241 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     3301 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     3361 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     3421 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     3481 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     3541 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     3601 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     3661 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     3721 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     3781 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     3841 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     3901 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
     3961 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg
     4021 ggcagtgagc gcaacgcaat taatacgcgt accgctagcc aggaagagtt tgtagaaacg
     4081 caaaaaggcc atccgtcagg atggccttct gcttagtttg atgcctggca gtttatggcg
     4141 ggcgtcctgc ccgccaccct ccgggccgtt gcttcacaac gttcaaatcc gctcccggcg
     4201 gatttgtcct actcaggaga gcgttcaccg acaaacaaca gataaaacga aaggcccagt
     4261 cttccgactg agcctttcgt tttatttgat gcctggcagt tccctactct cgcgttaacg
     4321 ctagcatgga tgttttccca gtcacgacgt tgtaaaacga cggccagtct taagctcggg
     4381 ccc
//
