LOCUS       sequence-376753-        4149 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4149)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 4149)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLC18A3_STOP
FEATURES             Location/Qualifiers
     source          1..4149
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1706..1805)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1822..1841)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1823..1841)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1846..1862)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1846..1862)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1964..1983)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1975..2784
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2042..2061)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2931..3519
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3420..3439
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3673..3690
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3849..3876)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3968..4054)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4103..4125
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4117..4134
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4118..4134
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacacat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccaccatgga aagcgccgag
      121 cccgccggac aggccagagc cgccgccact aaactgagcg aagccgtcgg agccgcactg
      181 caggaaccca ggagacagag gcggctggtg ctggtaatcg tgtgcgtggc cctgctgctg
      241 gacaacatgc tgtatatggt aatcgtgccg atcgtccccg actatatagc gcacatgaga
      301 ggcggcggcg agggcccaac cagaacacca gaggtgtggg agccaacact gccactgccc
      361 acaccagcca acgccagcgc ctacaccgcc aatacatctg cctccccaac agccgcctgg
      421 cccgccggca gcgccctgag accaaggtac cctacagaga gcgaggatgt gaagatcggc
      481 gtgctgtttg cctccaaggc catcctgcag ctgctggtga atccactgag cggcccattc
      541 atcgacagga tgtcctacga tgtgcccctg ctgatcggcc tgggcgtgat gttcgcctct
      601 acagtgctgt tcgcctttgc cgaggattac gccacactgt ttgccgccag gagcctgcag
      661 ggcctgggct ccgccttcgc cgataccagc ggcatcgcca tgatcgccga taagtaccct
      721 gaggagccag agagatctag ggccctgggc gtggccctgg cctttatcag cttcggctct
      781 ctggtggccc ctccatttgg cggcatcctg tacgagtttg ccggcaagag agtgccattt
      841 ctggtgctgg ccgccgtgag cctgtttgac gccctgctgc tgctggccgt ggccaagcct
      901 ttctctgccg ccgccagggc cagagccaat ctgcccgtgg gcaccccaat ccacagactg
      961 atgctggatc cttacatcgc cgtggtggcc ggcgccctga ccacatgcaa catccccctg
     1021 gcctttctgg agcctaccat cgccacatgg atgaagcaca ccatggccgc cagcgagtgg
     1081 gagatgggca tggcctggct gcctgccttt gtgcctcacg tgctgggcgt gtacctgaca
     1141 gtgaggctgg ccgccaggta tccacacctg cagtggctgt atggcgccct gggcctggcc
     1201 gtgatcggcg cctcctcttg tatcgtgccc gcctgcagat cttttgcccc actggtggtg
     1261 tctctgtgcg gcctgtgctt cggcatcgcc ctggtggata cagccctgct gccaaccctg
     1321 gccttcctgg tggacgtgag acacgtgagc gtgtatggct ccgtgtatgc catcgccgac
     1381 atctcttatt ccgtggccta cgccctgggc cctatcgtgg ccggccacat cgtgcactct
     1441 ctgggcttcg agcagctgtc tctgggcatg ggcctggcca atctgctgta tgccccagtg
     1501 ctgctgctgc tgaggaacgt gggcctgctg acaagatccc gctctgagag ggacgtgctg
     1561 ctggatgagc ctccccaggg cctgtacgat gccgtgagac tgagagagag accagtgtct
     1621 ggccaggatg gcgagccacg gagcccacct ggaccatttg acgcttgcga agatgactac
     1681 aactactatt acacccgaag ttaagaccca gctttcttgt acaaagttgg cattataaga
     1741 aagcattgct tatcaatttg ttgcaacgaa caggtcacta tcagtcaaaa taaaatcatt
     1801 atttgccatc cagctgatat cccctatagt gagtcgtatt acatggtcat agctgtttcc
     1861 tggcagctct ggcccgtgtc tcaaaatctc tgatgttaca ttgcacaaga taaaaatata
     1921 tcatcatgaa caataaaact gtctgcttac ataaacagta atacaagggg tgttatgagc
     1981 catattcaac gggaaacgtc gaggccgcga ttaaattcca acatggatgc tgatttatat
     2041 gggtataaat gggctcgcga taatgtcggg caatcaggtg cgacaatcta tcgcttgtat
     2101 gggaagcccg atgcgccaga gttgtttctg aaacatggca aaggtagcgt tgccaatgat
     2161 gttacagatg agatggtcag actaaactgg ctgacggaat ttatgcctct tccgaccatc
     2221 aagcatttta tccgtactcc tgatgatgca tggttactca ccactgcgat ccccggaaaa
     2281 acagcattcc aggtattaga agaatatcct gattcaggtg aaaatattgt tgatgcgctg
     2341 gcagtgttcc tgcgccggtt gcattcgatt cctgtttgta attgtccttt taacagcgat
     2401 cgcgtatttc gtctcgctca ggcgcaatca cgaatgaata acggtttggt tgatgcgagt
     2461 gattttgatg acgagcgtaa tggctggcct gttgaacaag tctggaaaga aatgcataaa
     2521 cttttgccat tctcaccgga ttcagtcgtc actcatggtg atttctcact tgataacctt
     2581 atttttgacg aggggaaatt aataggttgt attgatgttg gacgagtcgg aatcgcagac
     2641 cgataccagg atcttgccat cctatggaac tgcctcggtg agttttctcc ttcattacag
     2701 aaacggcttt ttcaaaaata tggtattgat aatcctgata tgaataaatt gcagtttcat
     2761 ttgatgctcg atgagttttt ctaatcagaa ttggttaatt ggttgtaaca ctggcagagc
     2821 attacgctga cttgacggga cggcgcaagc tcatgaccaa aatcccttaa cgtgagttac
     2881 gcgtcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     2941 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     3001 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     3061 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     3121 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     3181 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     3241 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     3301 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     3361 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     3421 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     3481 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     3541 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     3601 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     3661 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
     3721 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg
     3781 aaagcgggca gtgagcgcaa cgcaattaat acgcgtaccg ctagccagga agagtttgta
     3841 gaaacgcaaa aaggccatcc gtcaggatgg ccttctgctt agtttgatgc ctggcagttt
     3901 atggcgggcg tcctgcccgc caccctccgg gccgttgctt cacaacgttc aaatccgctc
     3961 ccggcggatt tgtcctactc aggagagcgt tcaccgacaa acaacagata aaacgaaagg
     4021 cccagtcttc cgactgagcc tttcgtttta tttgatgcct ggcagttccc tactctcgcg
     4081 ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc cagtcttaag
     4141 ctcgggccc
//
