LOCUS       sequence-37677-e        4688 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4688)
  AUTHORS   Villefranc JA, Amigo J, Lawson ND
  TITLE     Gateway compatible vectors for analysis of gene function in the 
            zebrafish.
  JOURNAL   Dev Dyn. 2007 Nov . 236(11):3077-87.
  PUBMED    17948311
REFERENCE   2  (bases 1 to 4688)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Dyn. 
            2007 Nov . 236(11):3077-87."
COMMENT     Sequence Label: 478 p5Efli1ep
FEATURES             Location/Qualifiers
     source          1..4688
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    593..688
                     /gene="mutant version of attL"
                     /label=attL4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    2734..2857
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2929..2948)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2930..2948)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2953..2969)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2953..2969)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3071..3090)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             3082..3891
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(3149..3168)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      4038..4626
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4527..4546
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccgc gttaacgcta ccatggagct ccaaataatg
      601 attttatttt gactgatagt gacctgttcg ttgcaacaaa ttgataagca atgctttttt
      661 ataatgccaa ctttgtatag aaaagttgga tctcatcttg acccataaac atacactaaa
      721 acccaacagt caactttatc aaatgaaatg agtgtaatta actcaaaatt tactgaatgt
      781 tgattctact cattcgaaaa gagttttaaa ctcagtgttg aaggtagtga gttcattaaa
      841 tacctcatta cttcaactta actggagtaa gttcatggta ctcacataga ttagtttagc
      901 tcaaatggtt tgtagcagtc ggttccctca agcagtttga gttgccttaa ctttttgggt
      961 tttacagtac tcagttggtt tgagttcact tcacttattg ggttcaacat gtgctcaaat
     1021 tgcatcgttt aatcaaatag attaagttca cagtactcat taggattagt ttttgaactt
     1081 aaatggtttg ttgcagtcgg tttcctcaaa cggtttgagt ttccataact ttatgggttt
     1141 ttcagtgtag taaacatatg catccacaga ttataatagt aacctgcatg ttttttcctt
     1201 cacaatttga cacagatacc tcacatgatt gacttttaac tgtacacttt agtcgacaat
     1261 gagaagggtg tctcaatcta tgggtatttc ctgttccaca caatctggtt tcctgtcagg
     1321 tttccttttc ctcctgaaac aaaagacagg aatgccctct gtataaaata gcacagcgct
     1381 tttaatgggt ctctgtacat cccgacctca tgacgcatct tctgcgggac agccaatcac
     1441 gatcaagttg gcttatgaaa acagtttttt gtccccctgt taagttaaaa gcacggtagc
     1501 atacatgcat tattcatcat gcagcatatt cagcagtaca aaagatgagc tcttgacaca
     1561 cctcagcgta aaatctctat tccagcgtaa tcctgcggtt ctggatggtt aagcgagcct
     1621 tgcaaacacg gccaatttat agccatgttg aaaatagcag ctttgtgagc atttcgacct
     1681 caatgtacca agaatgcgtg ctcggtaact gaaaaagagg gggtggagat tttggattta
     1741 gagatggaat cacatgcacg ctgtatatgc tatataaaaa tagcaggcat ttaatgcatg
     1801 caaaatatgt ttcgacacta aacactacaa gtcggaaata tatctatttt tcttttttta
     1861 acgaaatgag acatttaatg cgcagtaaat gtagtctaac acgcacaata ttcctattat
     1921 tgtccatgaa aaatgtgagg tcrtatgcta ctgatttaaa gaaaatgttg aacaggcaca
     1981 gcacacaaga ggtatcagcc tctctatatg gtccatacag caccaggctc gctgcgttca
     2041 tgtgcattca gaggcgcgca cacgtgtgga gcagacagac tcgacgcagc gggctttgct
     2101 ttttattcat ggaagcccat tttagaagaa gctcgggcag actcgtcaca ttcttcactt
     2161 caccataggt agatcaaatc aaagttattg gatataacgg aacaatattc atcatattat
     2221 gagattatta ttagaccgag tgcattagac atatttgtat tcgatcgttg aaaaagtata
     2281 tattttattt tagccttaaa ctatggttag cctatattaa tcagcataat ctgttatttc
     2341 agacgttgta ttaatgatta tttctagcac agctttgatg catcaatgca atgtttctac
     2401 aaacccgcct tgtttaatgt cggaaaaaaa gcgcaacaaa tgtaaagagt tggcaggcat
     2461 tctgtaaata tttccaggca aaaacatgca caaaggaaag aaacaccatt gttttggtgt
     2521 cttatcttcg ctggacaaat atcctgcttt gggtgccgta atgaaggcag ggccagtgac
     2581 acacacacat acacacacac acatacacgc atacacggct tccttctcaa gcgcagtgac
     2641 ttcacttccc aaatttagag gaaaaaaaac catccggcga aactgtctct gtctctccgc
     2701 cacatatcgg gggctggaat taattcagac gcgcaagttt gtacaaaaaa gttgaacgag
     2761 aaacgtaaaa tgatataaat atcaatatat taaattagat tttgcataaa aaacagacta
     2821 cataatactg taaaacacaa catatgcagt cactatgaac caactactta gatggtatta
     2881 gtgacctgta gaattcgagc tctagagctg cagggcggcc gcgatatccc ctatagtgag
     2941 tcgtattaca tggtcatagc tgtttcctgg cagctctggc ccgtgtctca aaatctctga
     3001 tgttacattg cacaagataa aaatatatca tcatgaacaa taaaactgtc tgcttacata
     3061 aacagtaata caaggggtgt tatgagccat attcaacggg aaacgtcgag gccgcgatta
     3121 aattccaaca tggatgctga tttatatggg tataaatggg ctcgcgataa tgtcgggcaa
     3181 tcaggtgcga caatctatcg cttgtatggg aagcccgatg cgccagagtt gtttctgaaa
     3241 catggcaaag gtagcgttgc caatgatgtt acagatgaga tggtcagact aaactggctg
     3301 acggaattta tgcctcttcc gaccatcaag cattttatcc gtactcctga tgatgcatgg
     3361 ttactcacca ctgcgatccc cggaaaaaca gcattccagg tattagaaga atatcctgat
     3421 tcaggtgaaa atattgttga tgcgctggca gtgttcctgc gccggttgca ttcgattcct
     3481 gtttgtaatt gtccttttaa cagcgatcgc gtatttcgtc tcgctcaggc gcaatcacga
     3541 atgaataacg gtttggttga tgcgagtgat tttgatgacg agcgtaatgg ctggcctgtt
     3601 gaacaagtct ggaaagaaat gcataaactt ttgccattct caccggattc agtcgtcact
     3661 catggtgatt tctcacttga taaccttatt tttgacgagg ggaaattaat aggttgtatt
     3721 gatgttggac gagtcggaat cgcagaccga taccaggatc ttgccatcct atggaactgc
     3781 ctcggtgagt tttctccttc attacagaaa cggctttttc aaaaatatgg tattgataat
     3841 cctgatatga ataaattgca gtttcatttg atgctcgatg agtttttcta atcagaattg
     3901 gttaattggt tgtaacactg gcagagcatt acgctgactt gacgggacgg cgcaagctca
     3961 tgaccaaaat cccttaacgt gagttacgcg tcgttccact gagcgtcaga ccccgtagaa
     4021 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     4081 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     4141 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg
     4201 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     4261 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     4321 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     4381 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagca ttgagaaagc
     4441 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     4501 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     4561 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     4621 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     4681 cacatgtt
//
