LOCUS       sequence-376791-        2295 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Engineered PcrA_M6 helicase to support isothermal amplification 
            method SHARP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2295)
  AUTHORS   Gavrilov M, Yang JYC, Zou RS, Ma W, Lee CY, Mohapatra S, Kang J, 
            Liao TW, Myong S, Ha T
  TITLE     Engineered helicase replaces thermocycler in DNA amplification while
            retaining desired PCR characteristics.
  JOURNAL   Nat Commun. 2022 Oct 23;13(1):6312. doi: 10.1038/s41467-022-34076-0.
  PUBMED    36274095
REFERENCE   2  (bases 1 to 2295)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-10-23"; volume: "13(1):6312. doi"; pages: " 
            10.1038/s41467-022-34076-0"
COMMENT     Sequence Label: pET-11b_PcrA_M6_H93A
FEATURES             Location/Qualifiers
     source          1..2295
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..24
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             2215..2286
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags"
                     /label=3xFLAG
                     /translation="DYKDDDDKDYKDDDDKDYKDDDDK"
ORIGIN
        1 catatgcatc accatcacca tcacgggtcc ggaatgaact ttttatcgga acagctgctc
       61 gcccatttaa acaaagagca acaagaagcc gtcaggacga cggaaggccc gctgctcatt
      121 atggcggggg cgggaagcgg gaaaacgcgg gtgttgacgc accgcatcgc ctatttgatg
      181 gcggaaaagc atgtggcgcc gtggaacatt ttggccatta cgtttacgaa caaggcggcg
      241 cgcgaaatgc gggaacgtgt gcagtcgctc ttaggtgggg cggcggaaga cgtctggatt
      301 tcgacgttcg cgtcgatggc cgtccgcatt ttgcgccgcg acattgaccg catcggcatc
      361 aaccgcaatt tttccatcct tgatccgacg gaccagcttt cagtcatgaa aacgatttta
      421 aaagaaaaaa acatagaccc gaaaaaattt gagccgcgga cgattttagg cacgatcagc
      481 gcggcgaaaa acgagctgtt gcctccggag caattcgcga agcgggcctc gacgtattac
      541 gaaaaagtcg tcagcgatgt gtatcaagaa taccaacagc gcctgcttcg ctgtcattcg
      601 ctcgattttg acgatttgat catgacgacg atccaactgt ttgaccgcgt gccggatgtg
      661 cttcactatt accaatataa gtttcagtac attcatattg atgagtacca ggatacgaac
      721 cgcgctcaat atacgctggt caaaaagctg gcggaacgct ttcaaaacat tgccgccgtc
      781 ggcgacgccg accaatcgat ttatcggtgg cgcggggcgg acatccaaaa cattttgtcg
      841 ttcgagcgcg actatccgaa cgcaaaagtc attttgcttg aacaaaacta ccgctcgacg
      901 aagcgcattt tgcaagcggc gaacgaagtc atcgagcata acgtcaaccg gaagccgaaa
      961 cggctttgga cggaaaaccc ggaaggaaag ccgattcttt attatgaggc gatgaacgaa
     1021 gcggacgaag cgcagtttgt cgctggacgc atccgcgagg cggtggagcg cggcgaacgc
     1081 cgctaccgtg attttgctgt cttgtaccgg acgaacgccc agtcgcgtgt catggaggaa
     1141 atgttgctga aagcgaacat tccgtatcaa attgtcggcg gcgtaaagtt ctatgaccgg
     1201 aaagaaatta aagacattct cgcctatttg cgcgtcattg ccaatccgga cgatgattgc
     1261 agcttgcttc gcatcattaa cgtgccaaaa cgcggcattg gcgcctcgac gatcgacaaa
     1321 ctcgtccgct atgcagccga tcatgagctg tccttgtttg aggcgctcgg cgagctagag
     1381 atgatcgggc ttggcgccaa agcggccggg gcgctcgccg cgttccgcag ccagctcgag
     1441 caatggacac agctgcaaga atacgtctcc gtcaccgaac tcgtcgaaga agtgctcgac
     1501 aaatcgggct accgcgagat gctcaaggcg gagcggacga ttgaagcaca aagccggctc
     1561 gagaacttgg atgagttttt gtcggtgacg aagcattttg aaaatgtgag cgacgataaa
     1621 tcgctcatcg cctttttaac cgacttggcg ctcatttccg atttggacga gctgaacggg
     1681 acggaacagg ccgctgaagg agatgccgtc atgttgatga cgttgcatgc cgccaaaggg
     1741 ctcgagtttc cggtcgtctt tttgatcggc atggaagaag gcattttccc gcacaaccgc
     1801 tctctcgagg atgacgatga gatggaagaa gaacggcggc tggcgtacgt cggcatcacc
     1861 cgcgcggagg aagaacttgt gctgacgagc gcgcaaatgc ggacgttgtt tggcaacatc
     1921 caaatgaacc cgccgtcgcg ctttttgaat gaaattccgg cgcatttgct tgagacagcc
     1981 tcgcgccgcc aagcgggcgc ctcccgcccg gccgtttcgc gcccgcaggc aagcggcgcc
     2041 gtgggatcgt ggaaagtcgg cgatcgggcg aatcaccgga aatggggcat cggcaccgtc
     2101 gtcagcgtcc gcggcggcgg cgacgaccaa gagctcgaca tcgccttccc gagcccgatc
     2161 ggcattaaac ggttgcttgc caaatttgcg ccgattgaga aagtgggtag cggcgactac
     2221 aaggacgatg acgataagga ttataaagac gatgacgata aggactacaa agacgatgac
     2281 gataaatagg gatcc
//
