LOCUS       sequence-376799-       12911 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for inducible Fluc expression with 4x LexA 
            operator (LexAO) and constitutive Rluc and mCherry..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12911)
  AUTHORS   Huang Z, Wu Y, Allen ME, Pan Y, Kyriakakis P, Lu S, Chang YJ, Wang 
            X, Chien S, Wang Y
  TITLE     Engineering light-controllable CAR T cells for cancer immunotherapy.
  JOURNAL   Sci Adv. 2020 Feb 19;6(8):eaay9209. doi: 10.1126/sciadv.aay9209. 
            eCollection 2020 Feb.
  PUBMED    32128416
REFERENCE   2  (bases 1 to 12911)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2020-02-19"; volume: "6(8):eaay9209. doi"; pages: " 
            10.1126/sciadv.aay9209. eCollection 2020 Feb"
COMMENT     Sequence Label: pHR-LexAOx4-Pmin-Fluc-PGK-Rluc-P2A-mCherry
FEATURES             Location/Qualifiers
     source          1..12911
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             92..325
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(350..368)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(351..368)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(658..677)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     1010..1032
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1070..1088)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1156..1260
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1261..1329,1330..2121)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1479..1498)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2292..2880
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2781..2800
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     complement(3121..3141)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3126..3455
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3306..3441
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          4589..4654
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             4784..4804
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    5229..5363
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5266..5285)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5320..5339
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             5531..6164
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    6211..6336
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6833..7066
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7251..7295
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7580..7697
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    7780..7920
                     /label=bom
                     /note="basis of mobility region from pBR322"
     CDS             8270..9922
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(8336..8354)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     promoter        9933..10432
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(9968..9991)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     10365..10384
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             10453..11385
                     /codon_start=1
                     /product="Renilla luciferase"
                     /label=hRluc
                     /note="human codon-optimized"
                     /translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     CDS             11395..11451
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             11461..11517
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             11518..12228
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(11663..11681)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     11931..11950
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    12305..12893
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(12358..12378)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(12776..12787)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(join(12896..12911,1))
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(join(12897..12911,1..2))
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
ORIGIN
        1 gagggaatta attcgagctc ggtaccttta agaccaatga cttacaaggc agctgtagat
       61 cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga
      121 caagatctgc tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag
      181 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
      241 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
      301 tagtcagtgt ggaaaatctc tagcagcatc tagaattaat tccgtgtatt ctatagtgtc
      361 acctaaatcg tatgtgtatg atacataagg ttatgtatta attgtagccg cgttctaacg
      421 acaatatgta caagcctaat tgtgtagcat ctggcttact gaagcagacc ctatcatctc
      481 tctcgtaaac tgccgtcaga gtcggtttgg ttggacgaac cttctgagtt tctggtaacg
      541 ccgtcccgca cccggaaatg gtcagcgaac caatcagcag ggtcatcgct agccagatcc
      601 tctacgccgg acgcatcgtg gccggcatca ccggcgccac aggtgcggtt gctggcgcct
      661 atatcgccga catcaccgat ggggaagatc gggctcgcca cttcgggctc atgagcgctt
      721 gtttcggcgt gggtatggtg gcaggccccg tggccggggg actgttgggc gccatctcct
      781 tgcatgcacc attccttgcg gcggcggtgc tcaacggcct caacctacta ctgggctgct
      841 tcctaatgca ggagtcgcat aagggagagc gtcgaatggt gcactctcag tacaatctgc
      901 tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga
      961 cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc
     1021 atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata
     1081 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact
     1141 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     1201 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     1261 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     1321 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     1381 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     1441 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     1501 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     1561 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     1621 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     1681 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     1741 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     1801 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     1861 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     1921 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     1981 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     2041 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     2101 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     2161 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     2221 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     2281 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     2341 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     2401 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     2461 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     2521 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     2581 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     2641 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     2701 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     2761 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     2821 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     2881 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     2941 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     3001 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     3061 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgt
     3121 ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     3181 aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag
     3241 gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc
     3301 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
     3361 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt
     3421 gaggaggctt ttttggaggc ctaggctttt gcaaaaagct tggacacaag acaggcttgc
     3481 gagatatgtt tgagaatacc actttatccc gcgtcaggga gaggcagtgc gtaaaaagac
     3541 gcggactcat gtgaaatact ggtttttagt gcgccagatc tctataatct cgcgcaacct
     3601 attttcccct cgaacacttt ttaagccgta gataaacagg ctgggacact tcacatgagc
     3661 gaaaaataca tcgtcacctg ggacatgttg cagatccatg cacgtaaact cgcaagccga
     3721 ctgatgcctt ctgaacaatg gaaaggcatt attgccgtaa gccgtggcgg tctgtaccgg
     3781 gtgcgttact ggcgcgtgaa ctgggtattc gtcatgtcga taccgtttgt atttccagct
     3841 acgatcacga caaccagcgc gagcttaaag tgctgaaacg cgcagaaggc gatggcgaag
     3901 gcttcatcgt tattgatgac ctggtggata ccggtggtac tgcggttgcg attcgtgaaa
     3961 tgtatccaaa agcgcacttt gtcaccatct tcgcaaaacc ggctggtcgt ccgctggttg
     4021 atgactatgt tgttgatatc ccgcaagata cctggattga acagccgtgg gatatgggcg
     4081 tcgtattcgt cccgccaatc tccggtcgct aatcttttca acgcctggca ctgccgggcg
     4141 ttgttctttt taacttcagg cgggttacaa tagtttccag taagtattct ggaggctgca
     4201 tccatgacac aggcaaacct gagcgaaacc ctgttcaaac cccgctttaa acatcctgaa
     4261 acctcgacgc tagtccgccg ctttaatcac ggcgcacaac cgcctgtgca gtcggccctt
     4321 gatggtaaaa ccatccctca ctggtatcgc atgattaacc gtctgatgtg gatctggcgc
     4381 ggcattgacc cacgcgaaat cctcgacgtc caggcacgta ttgtgatgag cgatgccgaa
     4441 cgtaccgacg atgatttata cgatacggtg attggctacc gtggcggcaa ctggatttat
     4501 gagtgggccc cggatctttg tgaaggaacc ttacttctgt ggtgtgacat aattggacaa
     4561 actacctaca gagatttaaa gctctaaggt aaatataaaa tttttaagtg tataatgtgt
     4621 taaactactg attctaattg tttgtgtatt ttagattcca acctatggaa ctgatgaatg
     4681 ggagcagtgg tggaatgcct ttaatgagga aaacctgttt tgctcagaag aaatgccatc
     4741 tagtgatgat gaggctactg ctgactctca acattctact cctccaaaaa agaagagaaa
     4801 ggtagaagac cccaaggact ttccttcaga attgctaagt tttttgagtc atgctgtgtt
     4861 tagtaataga actcttgctt gctttgctat ttacaccaca aaggaaaaag ctgcactgct
     4921 atacaagaaa attatggaaa aatattctgt aacctttata agtaggcata acagttataa
     4981 tcataacata ctgttttttc ttactccaca caggcataga gtgtctgcta ttaataacta
     5041 tgctcaaaaa ttgtgtacct ttagcttttt aatttgtaaa ggggttaata aggaatattt
     5101 gatgtatagt gccttgacta gagatcataa tcagccatac cacatttgta gaggttttac
     5161 ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg
     5221 ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     5281 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     5341 atgtatctta tcatgtctgg atcaactgga taactcaagc taaccaaaat catcccaaac
     5401 ttcccacccc ataccctatt accactgcca attacctgtg gtttcattta ctctaaacct
     5461 gtgattcctc tgaattattt tcattttaaa gaaattgtat ttgttaaata tgtactacaa
     5521 acttagtagt tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg
     5581 atctaccaca cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc
     5641 agatatccac tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta
     5701 gaagaggcca ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg
     5761 gatgacccgg agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac
     5821 gtggcccgag agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag
     5881 ggactttccg ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga
     5941 gccctcagat cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga
     6001 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     6061 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     6121 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg
     6181 gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa
     6241 gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc
     6301 ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga
     6361 tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat
     6421 atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca
     6481 tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa
     6541 gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag
     6601 ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc
     6661 accgcacagc aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca
     6721 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     6781 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     6841 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     6901 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     6961 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     7021 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     7081 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
     7141 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
     7201 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
     7261 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
     7321 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
     7381 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
     7441 agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg
     7501 gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccaaa
     7561 tggcagtatt catccacaat tttaaaagaa aaggggggat tggggggtac agtgcagggg
     7621 aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta
     7681 caaaaattca aaattttcgg gtttattaca gggacagcag agatccagtt tggatcgata
     7741 agcttgatat cagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct
     7801 tacgcatctg tgcggtattt cacaccgcat atggtgcact ctcagtacaa tctgctctga
     7861 tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg
     7921 ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc
     7981 gcttacagac aagctgtgac cgggtaccga attcgcggcc gcatctagac tgtatataaa
     8041 accagtggtt atatgtacag actagactgt atataaaacc agtggttata tgtacagact
     8101 agactgtata taaaaccagt ggttatatgt acagactaga ctgtatataa aaccagtggt
     8161 tatatgtaca gactagactc gagtccgagc ggagactcta gagggtatat aatggatccc
     8221 cgggtaccga gctcgaattc cagcttggca ttccggtact gttggtaaaa tggaagacgc
     8281 caaaaacata aagaaaggcc cggcgccatt ctatccgctg gaagatggaa ccgctggaga
     8341 gcaactgcat aaggctatga agagatacgc cctggttcct ggaacaattg cttttacaga
     8401 tgcacatatc gaggtggaca tcacttacgc tgagtacttc gaaatgtccg ttcggttggc
     8461 agaagctatg aaacgatatg ggctgaatac aaatcacaga atcgtcgtat gcagtgaaaa
     8521 ctctcttcaa ttctttatgc cggtgttggg cgcgttattt atcggagttg cagttgcgcc
     8581 cgcgaacgac atttataatg aacgtgaatt gctcaacagt atgggcattt cgcagcctac
     8641 cgtggtgttc gtttccaaaa aggggttgca aaaaattttg aacgtgcaaa aaaagctccc
     8701 aatcatccaa aaaattatta tcatggattc taaaacggat taccagggat ttcagtcgat
     8761 gtacacgttc gtcacatctc atctacctcc cggttttaat gaatacgatt ttgtgccaga
     8821 gtccttcgat agggacaaga caattgcact gatcatgaac tcctctggat ctactggtct
     8881 gcctaaaggt gtcgctctgc ctcatagaac tgcctgcgtg agattctcgc atgccagaga
     8941 tcctattttt ggcaatcaaa tcattccgga tactgcgatt ttaagtgttg ttccattcca
     9001 tcacggtttt ggaatgttta ctacactcgg atatttgata tgtggatttc gagtcgtctt
     9061 aatgtataga tttgaagaag agctgtttct gaggagcctt caggattaca agattcaaag
     9121 tgcgctgctg gtgccaaccc tattctcctt cttcgccaaa agcactctga ttgacaaata
     9181 cgatttatct aatttacacg aaattgcttc tggtggcgct cccctctcta aggaagtcgg
     9241 ggaagcggtt gccaagaggt tccatctgcc aggtatcagg caaggatatg ggctcactga
     9301 gactacatca gctattctga ttacacccga gggggatgat aaaccgggcg cggtcggtaa
     9361 agttgttcca ttttttgaag cgaaggttgt ggatctggat accgggaaaa cgctgggcgt
     9421 taatcaaaga ggcgaactgt gtgtgagagg tcctatgatt atgtccggtt atgtaaacaa
     9481 tccggaagcg accaacgcct tgattgacaa ggatggatgg ctacattctg gagacatagc
     9541 ttactgggac gaagacgaac acttcttcat cgttgaccgc ctgaagtctc tgattaagta
     9601 caaaggctat caggtggctc ccgctgaatt ggaatccatc ttgctccaac accccaacat
     9661 cttcgacgca ggtgtcgcag gtcttcccga cgatgacgcc ggtgaacttc ccgccgccgt
     9721 tgttgttttg gagcacggaa agacgatgac ggaaaaagag atcgtggatt acgtcgccag
     9781 tcaagtaaca accgcgaaaa agttgcgcgg aggagttgtg tttgtggacg aagtaccgaa
     9841 aggtcttacc ggaaaactcg acgcaagaaa aatcagagag atcctcataa aggccaagaa
     9901 gggcggaaag atcgccgtgt aagaattcta ccgggtaggg gaggcgcttt tcccaaggca
     9961 gtctggagca tgcgctttag cagccccgct gggcacttgg cgctacacaa gtggcctctg
    10021 gcctcgcaca cattccacat ccaccggtag gcgccaaccg gctccgttct ttggtggccc
    10081 cttcgcgcca ccttctactc ctcccctagt caggaagttc ccccccgccc cgcagctcgc
    10141 gtcgtgcagg acgtgacaaa tggaagtagc acgtctcact agtctcgtgc agatggacag
    10201 caccgctgag caatggaagc gggtaggcct ttggggcagc ggccaatagc agctttgctc
    10261 cttcgctttc tgggctcaga ggctgggaag gggtgggtcc gggggcgggc tcaggggcgg
    10321 gctcaggggc ggggcgggcg cccgaaggtc ctccggaggc ccggcattct gcacgcttca
    10381 aaagcgcacg tctgccgcgc tgttctcctc ttcctcatct ccgggccttt cgacctgcag
    10441 cccaagctta ccatggcttc caaggtgtac gaccccgagc aacgcaaacg catgatcact
    10501 gggcctcagt ggtgggctcg ctgcaagcaa atgaacgtgc tggactcctt catcaactac
    10561 tatgattccg agaagcacgc cgagaacgcc gtgatttttc tgcatggtaa cgctgcctcc
    10621 agctacctgt ggaggcacgt cgtgcctcac atcgagcccg tggctagatg catcatccct
    10681 gatctgatcg gaatgggtaa gtccggcaag agcgggaatg gctcatatcg cctcctggat
    10741 cactacaagt acctcaccgc ttggttcgag ctgctgaacc ttccaaagaa aatcatcttt
    10801 gtgggccacg actggggggc ttgtctggcc tttcactact cctacgagca ccaagacaag
    10861 atcaaggcca tcgtccatgc tgagagtgtc gtggacgtga tcgagtcctg ggacgagtgg
    10921 cctgacatcg aggaggatat cgccctgatc aagagcgaag agggcgagaa aatggtgctt
    10981 gagaataact tcttcgtcga gaccatgctc ccaagcaaga tcatgcggaa actggagcct
    11041 gaggagttcg ctgcctacct ggagccattc aaggagaagg gcgaggttag acggcctacc
    11101 ctctcctggc ctcgcgagat ccctctcgtt aagggaggca agcccgacgt cgtccagatt
    11161 gtccgcaact acaacgccta ccttcgggcc agcgacgatc tgcctaagat gttcatcgag
    11221 tccgaccctg ggttcttttc caacgctatt gtcgagggag ctaagaagtt ccctaacacc
    11281 gagttcgtga aggtgaaggg cctccacttc agccaggagg acgctccaga tgaaatgggt
    11341 aagtacatca agagcttcgt ggagcgcgtg ctgaagaacg agcagggaag cggagctact
    11401 aacttcagcc tgctgaagca ggctggagac gtggaggaga accctggacc tggttccggc
    11461 gcaacaaact tctctctgct gaaacaagcc ggagatgtcg aagagaatcc tggaccgatg
    11521 gtgagcaagg gcgaggagga taacatggcc atcatcaagg agttcatgcg cttcaaggtg
    11581 cacatggagg gctccgtgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc
    11641 ccctacgagg gcacccagac cgccaagctg aaggtgacca agggtggccc cctgcccttc
    11701 gcctgggaca tcctgtcccc tcagttcatg tacggctcca aggcctacgt gaagcacccc
    11761 gccgacatcc ccgactactt gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg
    11821 atgaacttcg aggacggcgg cgtggtgacc gtgacccagg actcctccct gcaggacggc
    11881 gagttcatct acaaggtgaa gctgcgcggc accaacttcc cctccgacgg ccccgtaatg
    11941 cagaagaaga ccatgggctg ggaggcctcc tccgagcgga tgtaccccga ggacggcgcc
    12001 ctgaagggcg agatcaagca gaggctgaag ctgaaggacg gcggccacta cgacgctgag
    12061 gtcaagacca cctacaaggc caagaagccc gtgcagctgc ccggcgccta caacgtcaac
    12121 atcaagttgg acatcacctc ccacaacgag gactacacca tcgtggaaca gtacgaacgc
    12181 gccgagggcc gccactccac cggcggcatg gacgagctgt acaagtaaag cggccgcgac
    12241 tctagagtcg acctgcaggc atgcaagctt gcaggcatgc aagcttgata tcaagcttat
    12301 cgataatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt
    12361 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc
    12421 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga
    12481 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc
    12541 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct
    12601 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg
    12661 gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct
    12721 gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc
    12781 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg
    12841 tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcatcgata
    12901 ccgtcgacct c
//
