LOCUS       sequence-376800-       13019 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for constitutive expression of eGFP-P2A-CRY2-VPR..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13019)
  AUTHORS   Huang Z, Wu Y, Allen ME, Pan Y, Kyriakakis P, Lu S, Chang YJ, Wang 
            X, Chien S, Wang Y
  TITLE     Engineering light-controllable CAR T cells for cancer immunotherapy.
  JOURNAL   Sci Adv. 2020 Feb 19;6(8):eaay9209. doi: 10.1126/sciadv.aay9209. 
            eCollection 2020 Feb.
  PUBMED    32128416
REFERENCE   2  (bases 1 to 13019)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2020-02-19"; volume: "6(8):eaay9209. doi"; pages: " 
            10.1126/sciadv.aay9209. eCollection 2020 Feb"
COMMENT     Sequence Label: pHR-PGK-eGFP-P2A-CRY2-VPR
FEATURES             Location/Qualifiers
     source          1..13019
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             88..321
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(346..364)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(347..364)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(654..673)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(887..906)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1006..1028
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1066..1084)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1152..1256
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1257..1325,1326..2117)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1475..1494)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2288..2876
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2777..2796
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3030..3047
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(3117..3137)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3122..3451
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3302..3437
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          4585..4650
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             4780..4800
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    5225..5359
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5262..5281)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5316..5335
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             5527..6160
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    6207..6332
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6829..7062
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7247..7291
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7576..7693
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        7753..8252
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(7788..7811)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     8185..8204
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     regulatory      8280..8289
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(8286..8288,8289..8291,8292..9002)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(8286..8288,8289..8291,8292..9002)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(8331..8352)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(8592..8611)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8939..8960
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             9012..9068
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             9072..9119
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             10620..10640
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    10686..10710
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             10740..10889
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             10914..10934
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             11364..11720
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALL"
     CDS             11739..12308
                     /codon_start=1
                     /product="transcriptional activation domain from the human 
                     herpesvirus 4 (Epstein-Barr virus) replication and 
                     transcription activator Rta/BRLF1 (Hardwick et al., 1992; 
                     Chavez et al., 2015)"
                     /label=Rta AD
                     /translation="RDSREGMFLPKPEAGSAISDVFEGREVCQPKRIRPFHPPGSPWAN
                     RPLPASLAPTPTGPVHEPVGSLTPAPVPQPLDPAPAVTPEASHLLEDPDEETSQAVKAL
                     REMADTVIPQKEEAAICGQMDLSHPPPRGHLDELTTTLESMTEDLNLDSPLTPELNEIL
                     DTFLNDECLLHAMHISTGLSIFDTSLF"
     misc_feature    12409..12997
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(12462..12482)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(12880..12891)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(13000..13016)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(13001..13017)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
ORIGIN
        1 gaattaattc gagctcggta cctttaagac caatgactta caaggcagct gtagatctta
       61 gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag
      121 atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct
      181 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
      241 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
      301 cagtgtggaa aatctctagc agcatctaga attaattccg tgtattctat agtgtcacct
      361 aaatcgtatg tgtatgatac ataaggttat gtattaattg tagccgcgtt ctaacgacaa
      421 tatgtacaag cctaattgtg tagcatctgg cttactgaag cagaccctat catctctctc
      481 gtaaactgcc gtcagagtcg gtttggttgg acgaaccttc tgagtttctg gtaacgccgt
      541 cccgcacccg gaaatggtca gcgaaccaat cagcagggtc atcgctagcc agatcctcta
      601 cgccggacgc atcgtggccg gcatcaccgg cgccacaggt gcggttgctg gcgcctatat
      661 cgccgacatc accgatgggg aagatcgggc tcgccacttc gggctcatga gcgcttgttt
      721 cggcgtgggt atggtggcag gccccgtggc cgggggactg ttgggcgcca tctccttgca
      781 tgcaccattc cttgcggcgg cggtgctcaa cggcctcaac ctactactgg gctgcttcct
      841 aatgcaggag tcgcataagg gagagcgtcg aatggtgcac tctcagtaca atctgctctg
      901 atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg
      961 cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt
     1021 gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc
     1081 tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc
     1141 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     1201 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     1261 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     1321 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     1381 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     1441 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
     1501 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     1561 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     1621 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     1681 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     1741 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     1801 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     1861 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     1921 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     1981 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     2041 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     2101 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     2161 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     2221 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     2281 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     2341 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     2401 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     2461 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     2521 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     2581 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     2641 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     2701 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     2761 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     2821 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     2881 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     2941 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     3001 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     3061 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctgtggaa
     3121 tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     3181 catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag
     3241 aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc
     3301 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     3361 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg
     3421 aggctttttt ggaggcctag gcttttgcaa aaagcttgga cacaagacag gcttgcgaga
     3481 tatgtttgag aataccactt tatcccgcgt cagggagagg cagtgcgtaa aaagacgcgg
     3541 actcatgtga aatactggtt tttagtgcgc cagatctcta taatctcgcg caacctattt
     3601 tcccctcgaa cactttttaa gccgtagata aacaggctgg gacacttcac atgagcgaaa
     3661 aatacatcgt cacctgggac atgttgcaga tccatgcacg taaactcgca agccgactga
     3721 tgccttctga acaatggaaa ggcattattg ccgtaagccg tggcggtctg taccgggtgc
     3781 gttactggcg cgtgaactgg gtattcgtca tgtcgatacc gtttgtattt ccagctacga
     3841 tcacgacaac cagcgcgagc ttaaagtgct gaaacgcgca gaaggcgatg gcgaaggctt
     3901 catcgttatt gatgacctgg tggataccgg tggtactgcg gttgcgattc gtgaaatgta
     3961 tccaaaagcg cactttgtca ccatcttcgc aaaaccggct ggtcgtccgc tggttgatga
     4021 ctatgttgtt gatatcccgc aagatacctg gattgaacag ccgtgggata tgggcgtcgt
     4081 attcgtcccg ccaatctccg gtcgctaatc ttttcaacgc ctggcactgc cgggcgttgt
     4141 tctttttaac ttcaggcggg ttacaatagt ttccagtaag tattctggag gctgcatcca
     4201 tgacacaggc aaacctgagc gaaaccctgt tcaaaccccg ctttaaacat cctgaaacct
     4261 cgacgctagt ccgccgcttt aatcacggcg cacaaccgcc tgtgcagtcg gcccttgatg
     4321 gtaaaaccat ccctcactgg tatcgcatga ttaaccgtct gatgtggatc tggcgcggca
     4381 ttgacccacg cgaaatcctc gacgtccagg cacgtattgt gatgagcgat gccgaacgta
     4441 ccgacgatga tttatacgat acggtgattg gctaccgtgg cggcaactgg atttatgagt
     4501 gggccccgga tctttgtgaa ggaaccttac ttctgtggtg tgacataatt ggacaaacta
     4561 cctacagaga tttaaagctc taaggtaaat ataaaatttt taagtgtata atgtgttaaa
     4621 ctactgattc taattgtttg tgtattttag attccaacct atggaactga tgaatgggag
     4681 cagtggtgga atgcctttaa tgaggaaaac ctgttttgct cagaagaaat gccatctagt
     4741 gatgatgagg ctactgctga ctctcaacat tctactcctc caaaaaagaa gagaaaggta
     4801 gaagacccca aggactttcc ttcagaattg ctaagttttt tgagtcatgc tgtgtttagt
     4861 aatagaactc ttgcttgctt tgctatttac accacaaagg aaaaagctgc actgctatac
     4921 aagaaaatta tggaaaaata ttctgtaacc tttataagta ggcataacag ttataatcat
     4981 aacatactgt tttttcttac tccacacagg catagagtgt ctgctattaa taactatgct
     5041 caaaaattgt gtacctttag ctttttaatt tgtaaagggg ttaataagga atatttgatg
     5101 tatagtgcct tgactagaga tcataatcag ccataccaca tttgtagagg ttttacttgc
     5161 tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt
     5221 tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     5281 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     5341 atcttatcat gtctggatca actggataac tcaagctaac caaaatcatc ccaaacttcc
     5401 caccccatac cctattacca ctgccaatta cctgtggttt catttactct aaacctgtga
     5461 ttcctctgaa ttattttcat tttaaagaaa ttgtatttgt taaatatgta ctacaaactt
     5521 agtagttgga agggctaatt cactcccaaa gaagacaaga tatccttgat ctgtggatct
     5581 accacacaca aggctacttc cctgattagc agaactacac accagggcca ggggtcagat
     5641 atccactgac ctttggatgg tgctacaagc tagtaccagt tgagccagat aaggtagaag
     5701 aggccaataa aggagagaac accagcttgt tacaccctgt gagcctgcat gggatggatg
     5761 acccggagag agaagtgtta gagtggaggt ttgacagccg cctagcattt catcacgtgg
     5821 cccgagagct gcatccggag tacttcaaga actgctgata tcgagcttgc tacaagggac
     5881 tttccgctgg ggactttcca gggaggcgtg gcctgggcgg gactggggag tggcgagccc
     5941 tcagatcctg catataagca gctgcttttt gcctgtactg ggtctctctg gttagaccag
     6001 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
     6061 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
     6121 tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact
     6181 tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc
     6241 gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag
     6301 gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc
     6361 gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag
     6421 tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag
     6481 aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac
     6541 ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa
     6601 aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg
     6661 cacagcaagc ggccggccgc tgatcttcag acctggagga ggagatatga gggacaattg
     6721 gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac
     6781 caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt
     6841 ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt
     6901 acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat
     6961 tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag
     7021 aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc
     7081 tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct
     7141 ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac
     7201 aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga
     7261 attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct
     7321 gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt
     7381 tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac
     7441 ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga
     7501 gagagacaga gacagatcca ttcgattagt gaacggatct cgacggtatc gccaaatggc
     7561 agtattcatc cacaatttta aaagaaaagg ggggattggg gggtacagtg caggggaaag
     7621 aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa
     7681 aattcaaaat tttcgggttt attacaggga cagcagagat ccagtttgga tcgataagct
     7741 tgatatcgaa ttgggtaggg gaggcgcttt tcccaaggca gtctggagca tgcgctttag
     7801 cagccccgct gggcacttgg cgctacacaa gtggcctctg gcctcgcaca cattccacat
     7861 ccaccggtag gcgccaaccg gctccgttct ttggtggccc cttcgcgcca ccttctactc
     7921 ctcccctagt caggaagttc ccccccgccc cgcagctcgc gtcgtgcagg acgtgacaaa
     7981 tggaagtagc acgtctcact agtctcgtgc agatggacag caccgctgag caatggaagc
     8041 gggtaggcct ttggggcagc ggccaatagc agctttgctc cttcgctttc tgggctcaga
     8101 ggctgggaag gggtgggtcc gggggcgggc tcaggggcgg gctcaggggc ggggcgggcg
     8161 cccgaaggtc ctccggaggc ccggcattct gcacgcttca aaagcgcacg tctgccgcgc
     8221 tgttctcctc ttcctcatct ccgggccttt cgaattcgga attgatcctt cgaactagtg
     8281 ccaccatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc
     8341 tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca
     8401 cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc
     8461 ccaccctcgt gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca
     8521 tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca
     8581 tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca
     8641 ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg
     8701 ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga
     8761 agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc
     8821 tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca
     8881 accactacct gagcacccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca
     8941 tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca
     9001 agggaagcgg agctactaac ttcagcctgc tgaagcaggc tggagacgtg gaggagaacc
     9061 ctggacctat gaaaaggccg gcggccacga aaaaggccgg ccaggcaaaa aagaaaaaga
     9121 agatggacaa aaagacgatt gtgtggttta gacgtgacct tcgcattgag gacaaccctg
     9181 cgttggcagc ggcagcgcat gaaggttcgg tattccctgt ctttatctgg tgtcccgaag
     9241 aggaggggca attctatccc ggtcgcgcgt cccgatggtg gatgaaacag tcgctcgcac
     9301 atcttagcca gtcgttgaag gcgctcggct ctgatctgac gctcatcaaa acacacaata
     9361 caatttctgc cattctggat tgcattaggg tgactggtgc caccaaagtt gtgttcaatc
     9421 acctttatga tcccgtaagc ctggtcaggg atcatacagt aaaagagaaa cttgtagaga
     9481 ggggaatcag cgtccaatcg tacaatgggg acttgttgta tgaaccgtgg gaaatctatt
     9541 gtgagaaagg caaacctttc acttccttca attcgtactg gaagaaatgc ctcgacatgt
     9601 ccatcgagtc agtcatgctg ccgccacctt ggagacttat gcccattact gctgcggcag
     9661 aggcgatctg ggcttgcagc atcgaagaac ttggtctgga aaacgaagcg gaaaaaccca
     9721 gtaacgcgct cctgacgcga gcgtggtcgc caggctggtc gaatgctgat aagctcctta
     9781 atgagtttat cgagaaacag ttgatcgact atgcgaagaa tagcaagaag gtggtgggaa
     9841 attccacttc cctcctgtcg ccgtatctcc actttgggga gattagcgtg aggcatgtgt
     9901 tccaatgtgc gcggatgaag caaatcattt gggctcgcga caaaaactct gaaggagagg
     9961 agtctgccga tttgtttttg agaggaatcg gccttaggga gtattcacga tacatctgct
    10021 tcaactttcc gtttacacat gaacaatccc tcctcagcca cctccgcttc tttccgtggg
    10081 acgcggacgt ggacaaattc aaggcctggc ggcaggggcg cacgggatac ccgttggtgg
    10141 atgcgggtat gagagagctg tgggcaacgg gttggatgca caaccgtatt cgagtcatcg
    10201 ttagtagctt tgccgttaag ttcctcttgt tgccctggaa atgggggatg aaatactttt
    10261 gggacacgct tctggatgcg gacttggagt gcgatattct tgggtggcag tacatttcgg
    10321 gttccatccc tgacgggcat gaacttgacc gattggataa ccctgcactg caaggagcaa
    10381 agtacgaccc ggagggagaa tacatcaggc agtggctccc agagctggcc cgtctgccca
    10441 ccgagtggat tcaccacccg tgggatgctc ccctgaccgt ccttaaagcc tcaggggtcg
    10501 aattggggac gaattacgcg aaacccatcg tcgatattga caccgcacga gaattgctgg
    10561 cgaaagccat ctcaagaaca cgcgaagcgc agattatgat cggagcagcg ccggctagcc
    10621 ccaagaagaa gagaaaggtg gaggccagct cgccagggat ccgtcgactt gacgcgttga
    10681 tatcaacaag tttgtacaaa aaagcaggct acaaagaggc cagcggttcc ggacgggctg
    10741 acgcattgga cgattttgat ctggatatgc tgggaagtga cgccctcgat gattttgacc
    10801 ttgacatgct tggttcggat gcccttgatg actttgacct cgacatgctc ggcagtgacg
    10861 cccttgatga tttcgacctg gacatgctga ttaactctag aagttccgga tctccgaaaa
    10921 agaaacgcaa agttggtagc cagtacctgc ccgacaccga cgaccggcac cggatcgagg
    10981 aaaagcggaa gcggacctac gagacattca agagcatcat gaagaagtcc cccttcagcg
    11041 gccccaccga ccctagacct ccacctagaa gaatcgccgt gcccagcaga tccagcgcca
    11101 gcgtgccaaa acctgccccc cagccttacc ccttcaccag cagcctgagc accatcaact
    11161 acgacgagtt ccctaccatg gtgttcccca gcggccagat ctctcaggcc tctgctctgg
    11221 ctccagcccc tcctcaggtg ctgcctcagg ctcctgctcc tgcaccagct ccagccatgg
    11281 tgtctgcact ggctcaggca ccagcacccg tgcctgtgct ggctcctgga cctccacagg
    11341 ctgtggctcc accagcccct aaacctacac aggccggcga gggcacactg tctgaagctc
    11401 tgctgcagct gcagttcgac gacgaggatc tgggagccct gctgggaaac agcaccgatc
    11461 ctgccgtgtt caccgacctg gccagcgtgg acaacagcga gttccagcag ctgctgaacc
    11521 agggcatccc tgtggcccct cacaccaccg agcccatgct gatggaatac cccgaggcca
    11581 tcacccggct cgtgacaggc gctcagaggc ctcctgatcc agctcctgcc cctctgggag
    11641 caccaggcct gcctaatgga ctgctgtctg gcgacgagga cttcagctct atcgccgata
    11701 tggatttctc agccttgctg ggctctggca gcggcagccg ggattccagg gaagggatgt
    11761 ttttgccgaa gcctgaggcc ggctccgcta ttagtgacgt gtttgagggc cgcgaggtgt
    11821 gccagccaaa acgaatccgg ccatttcatc ctccaggaag tccatgggcc aaccgcccac
    11881 tccccgccag cctcgcacca acaccaaccg gtccagtaca tgagccagtc gggtcactga
    11941 ccccggcacc agtccctcag ccactggatc cagcgcccgc agtgactccc gaggccagtc
    12001 acctgttgga ggatcccgat gaagagacga gccaggctgt caaagccctt cgggagatgg
    12061 ccgatactgt gattccccag aaggaagagg ctgcaatctg tggccaaatg gacctttccc
    12121 atccgccccc aaggggccat ctggatgagc tgacaaccac acttgagtcc atgaccgagg
    12181 atctgaacct ggactcaccc ctgaccccgg aattgaacga gattctggat accttcctga
    12241 acgacgagtg cctcttgcat gccatgcata tcagcacagg actgtccatc ttcgacacat
    12301 ctctgtttta agcggccgca actcccacct gcaacatgcg tgactgactg aggccgcgac
    12361 tctagagtcg acctgcaggc atgcaagctt gatatcaagc ttatcgataa tcaacctctg
    12421 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
    12481 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
    12541 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
    12601 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
    12661 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
    12721 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
    12781 aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc
    12841 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
    12901 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct
    12961 cagacgagtc ggatctccct ttgggccgcc tccccgcatc gataccgtcg acctcgagg
//
