LOCUS       sequence-37685-e         896 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  a phytochrome-based near-infrared fluorescent protein, also known as
            iRFP713, used for in vivo imaging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 896)
  AUTHORS   Filonov GS, Piatkevich KD, Ting LM, Zhang J, Kim K, Verkhusha VV
  TITLE     Bright and stable near-infrared fluorescent protein for in vivo 
            imaging.
  JOURNAL   Nat Biotechnol. 2011 Jul 17. doi: 10.1038/nbt.1918.
  PUBMED    21765402
REFERENCE   2  (bases 1 to 896)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2011 Jul 17. doi: 10.1038/nbt.1918."
COMMENT     Sequence Label: pBAD/His-B-iRFP
FEATURES             Location/Qualifiers
     source          1..896
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             25..42
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             46..78
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(82..90,91..105)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
ORIGIN
        1 gaggaattaa ccatgggggg ttctcatcat catcatcatc atggtatggc tagcatgact
       61 ggtggacagc aaatgggtcg ggatctgtac gacgatgacg ataaggatcc gagctcgaga
      121 tctatgacag aaggatccgt cgccaggcag cctgacctct tgacctgcga cgatgagccg
      181 atccatatcc ccggtgccat ccaaccgcat ggactgctgc tcgccctcgc cgccgacatg
      241 acgatcgttg ccggcagcga caaccttccc gaactcaccg gactggcgat cggcgccctg
      301 atcggccgct ctgcggccga tgtcttcgac tcggagacgc acaaccgtct gacgatcgcc
      361 ttggccgagc ccggggcggc cgtcggagca ccgatcactg tcggcttcac gatgcgaaag
      421 gacgcaggct tcatcggctc ctggcatcgc catgatcagc tcatcttcct cgagctcgag
      481 cctccccagc gggacgtcgc cgagccgcag gcgttcttcc gccgcaccaa cagcgccatc
      541 cgccgcctgc aggccgccga aaccttggaa agcgcctgcg ccgccgcggc gcaagaggtg
      601 cggaagatta ccggcttcga tcgggtgatg atctatcgct tcgcctccga cttcagcggc
      661 gaagtgatcg cagaggatcg gtgcgccgag gtcgagtcaa aactaggcct gcactatcct
      721 gcctcaaccg tgccggcgca ggcccgtcgg ctctatacca tcaacccggt acggatcatt
      781 cccgatatca attatcggcc ggtgccggtc accccagacc tcaatccggt caccgggcgg
      841 ccgattgatc ttagcttcgc catcctgcgc agcgtctcgc ccgtccatct ggaatt
//
