LOCUS       sequence-376878-       10721 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for constitutive expression of LexA-CIB1-biLINuS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10721)
  AUTHORS   Huang Z, Wu Y, Allen ME, Pan Y, Kyriakakis P, Lu S, Chang YJ, Wang 
            X, Chien S, Wang Y
  TITLE     Engineering light-controllable CAR T cells for cancer immunotherapy.
  JOURNAL   Sci Adv. 2020 Feb 19;6(8):eaay9209. doi: 10.1126/sciadv.aay9209. 
            eCollection 2020 Feb.
  PUBMED    32128416
REFERENCE   2  (bases 1 to 10721)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2020-02-19"; volume: "6(8):eaay9209. doi"; pages: " 
            10.1126/sciadv.aay9209. eCollection 2020 Feb"
COMMENT     Sequence Label: pHR-PGK-LexA-CIB1-biLINuS
FEATURES             Location/Qualifiers
     source          1..10721
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             80..313
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(338..356)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(339..356)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(646..665)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(879..898)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     998..1020
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1058..1076)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1144..1248
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1249..1317,1318..2109)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1467..1486)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2280..2868
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2769..2788
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3022..3039
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(3109..3129)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3114..3443
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3294..3429
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          4577..4642
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             4772..4792
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    5217..5351
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5254..5273)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5308..5327
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             5519..6152
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    6199..6324
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6821..7054
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7239..7283
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7568..7685
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        7745..8244
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(7780..7803)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     8177..8196
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             8278..8529
                     /codon_start=1
                     /gene="lexA (truncated)"
                     /product="DNA-binding domain of E. coli LexA"
                     /label=LexA BD
                     /note="binds the 16-bp palindromic sequence 
                     5'-CTGTATATATATACAG-3'"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAEE
                     HLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAA"
     misc_feature    10103..10691
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(10156..10176)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10574..10585)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(10694..10710)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10695..10711)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
ORIGIN
        1 tcgagctcgg tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt
       61 ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca agatctgctt
      121 tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
      181 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
      241 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
      301 aaaatctcta gcagcatcta gaattaattc cgtgtattct atagtgtcac ctaaatcgta
      361 tgtgtatgat acataaggtt atgtattaat tgtagccgcg ttctaacgac aatatgtaca
      421 agcctaattg tgtagcatct ggcttactga agcagaccct atcatctctc tcgtaaactg
      481 ccgtcagagt cggtttggtt ggacgaacct tctgagtttc tggtaacgcc gtcccgcacc
      541 cggaaatggt cagcgaacca atcagcaggg tcatcgctag ccagatcctc tacgccggac
      601 gcatcgtggc cggcatcacc ggcgccacag gtgcggttgc tggcgcctat atcgccgaca
      661 tcaccgatgg ggaagatcgg gctcgccact tcgggctcat gagcgcttgt ttcggcgtgg
      721 gtatggtggc aggccccgtg gccgggggac tgttgggcgc catctccttg catgcaccat
      781 tccttgcggc ggcggtgctc aacggcctca acctactact gggctgcttc ctaatgcagg
      841 agtcgcataa gggagagcgt cgaatggtgc actctcagta caatctgctc tgatgccgca
      901 tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg
      961 ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg
     1021 ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta
     1081 taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat
     1141 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     1201 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     1261 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     1321 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     1381 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     1441 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
     1501 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     1561 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     1621 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     1681 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     1741 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     1801 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     1861 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     1921 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     1981 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     2041 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     2101 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     2161 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     2221 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     2281 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     2341 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     2401 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     2461 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     2521 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     2581 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     2641 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     2701 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     2761 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     2821 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     2881 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     2941 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     3001 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     3061 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctgtgg aatgtgtgtc
     3121 agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     3181 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     3241 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc
     3301 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
     3361 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt
     3421 ttggaggcct aggcttttgc aaaaagcttg gacacaagac aggcttgcga gatatgtttg
     3481 agaataccac tttatcccgc gtcagggaga ggcagtgcgt aaaaagacgc ggactcatgt
     3541 gaaatactgg tttttagtgc gccagatctc tataatctcg cgcaacctat tttcccctcg
     3601 aacacttttt aagccgtaga taaacaggct gggacacttc acatgagcga aaaatacatc
     3661 gtcacctggg acatgttgca gatccatgca cgtaaactcg caagccgact gatgccttct
     3721 gaacaatgga aaggcattat tgccgtaagc cgtggcggtc tgtaccgggt gcgttactgg
     3781 cgcgtgaact gggtattcgt catgtcgata ccgtttgtat ttccagctac gatcacgaca
     3841 accagcgcga gcttaaagtg ctgaaacgcg cagaaggcga tggcgaaggc ttcatcgtta
     3901 ttgatgacct ggtggatacc ggtggtactg cggttgcgat tcgtgaaatg tatccaaaag
     3961 cgcactttgt caccatcttc gcaaaaccgg ctggtcgtcc gctggttgat gactatgttg
     4021 ttgatatccc gcaagatacc tggattgaac agccgtggga tatgggcgtc gtattcgtcc
     4081 cgccaatctc cggtcgctaa tcttttcaac gcctggcact gccgggcgtt gttcttttta
     4141 acttcaggcg ggttacaata gtttccagta agtattctgg aggctgcatc catgacacag
     4201 gcaaacctga gcgaaaccct gttcaaaccc cgctttaaac atcctgaaac ctcgacgcta
     4261 gtccgccgct ttaatcacgg cgcacaaccg cctgtgcagt cggcccttga tggtaaaacc
     4321 atccctcact ggtatcgcat gattaaccgt ctgatgtgga tctggcgcgg cattgaccca
     4381 cgcgaaatcc tcgacgtcca ggcacgtatt gtgatgagcg atgccgaacg taccgacgat
     4441 gatttatacg atacggtgat tggctaccgt ggcggcaact ggatttatga gtgggccccg
     4501 gatctttgtg aaggaacctt acttctgtgg tgtgacataa ttggacaaac tacctacaga
     4561 gatttaaagc tctaaggtaa atataaaatt tttaagtgta taatgtgtta aactactgat
     4621 tctaattgtt tgtgtatttt agattccaac ctatggaact gatgaatggg agcagtggtg
     4681 gaatgccttt aatgaggaaa acctgttttg ctcagaagaa atgccatcta gtgatgatga
     4741 ggctactgct gactctcaac attctactcc tccaaaaaag aagagaaagg tagaagaccc
     4801 caaggacttt ccttcagaat tgctaagttt tttgagtcat gctgtgttta gtaatagaac
     4861 tcttgcttgc tttgctattt acaccacaaa ggaaaaagct gcactgctat acaagaaaat
     4921 tatggaaaaa tattctgtaa cctttataag taggcataac agttataatc ataacatact
     4981 gttttttctt actccacaca ggcatagagt gtctgctatt aataactatg ctcaaaaatt
     5041 gtgtaccttt agctttttaa tttgtaaagg ggttaataag gaatatttga tgtatagtgc
     5101 cttgactaga gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa
     5161 acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact
     5221 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     5281 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     5341 atgtctggat caactggata actcaagcta accaaaatca tcccaaactt cccaccccat
     5401 accctattac cactgccaat tacctgtggt ttcatttact ctaaacctgt gattcctctg
     5461 aattattttc attttaaaga aattgtattt gttaaatatg tactacaaac ttagtagttg
     5521 gaagggctaa ttcactccca aagaagacaa gatatccttg atctgtggat ctaccacaca
     5581 caaggctact tccctgatta gcagaactac acaccagggc caggggtcag atatccactg
     5641 acctttggat ggtgctacaa gctagtacca gttgagccag ataaggtaga agaggccaat
     5701 aaaggagaga acaccagctt gttacaccct gtgagcctgc atgggatgga tgacccggag
     5761 agagaagtgt tagagtggag gtttgacagc cgcctagcat ttcatcacgt ggcccgagag
     5821 ctgcatccgg agtacttcaa gaactgctga tatcgagctt gctacaaggg actttccgct
     5881 ggggactttc cagggaggcg tggcctgggc gggactgggg agtggcgagc cctcagatcc
     5941 tgcatataag cagctgcttt ttgcctgtac tgggtctctc tggttagacc agatctgagc
     6001 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
     6061 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
     6121 acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga cttgaaagcg
     6181 aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc gcgcacggca
     6241 agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg aggctagaag
     6301 gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc gcgatgggaa
     6361 aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat agtatgggca
     6421 agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc agaaggctgt
     6481 agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca
     6541 ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat aaaagacacc
     6601 aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaccac cgcacagcaa
     6661 gcggccggcc gctgatcttc agacctggag gaggagatat gagggacaat tggagaagtg
     6721 aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa
     6781 agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt
     6841 tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca
     6901 gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc
     6961 aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg
     7021 ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac
     7081 tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga
     7141 tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa
     7201 tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg
     7261 aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata
     7321 taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac
     7381 tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc
     7441 caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga gagagagaca
     7501 gagacagatc cattcgatta gtgaacggat ctcgacggta tcgccaaatg gcagtattca
     7561 tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag
     7621 acataatagc aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa
     7681 attttcgggt ttattacagg gacagcagag atccagtttg gatcgataag cttgatatcg
     7741 aattgggtag gggaggcgct tttcccaagg cagtctggag catgcgcttt agcagccccg
     7801 ctgggcactt ggcgctacac aagtggcctc tggcctcgca cacattccac atccaccggt
     7861 aggcgccaac cggctccgtt ctttggtggc cccttcgcgc caccttctac tcctccccta
     7921 gtcaggaagt tcccccccgc cccgcagctc gcgtcgtgca ggacgtgaca aatggaagta
     7981 gcacgtctca ctagtctcgt gcagatggac agcaccgctg agcaatggaa gcgggtaggc
     8041 ctttggggca gcggccaata gcagctttgc tccttcgctt tctgggctca gaggctggga
     8101 aggggtgggt ccgggggcgg gctcaggggc gggctcaggg gcggggcggg cgcccgaagg
     8161 tcctccggag gcccggcatt ctgcacgctt caaaagcgca cgtctgccgc gctgttctcc
     8221 tcttcctcat ctccgggcct ttcgaattcc gacctgcagc ccaagcttac cgccaccatg
     8281 aaagcgttaa cggccaggca acaagaggtg tttgatctca tccgtgatca catcagccag
     8341 acaggtatgc cgccgacgcg tgcggaaatc gcgcagcgtt tggggttccg ttccccaaac
     8401 gcggctgaag aacatctgaa ggcgctggca cgcaaaggcg ttattgaaat tgtttccggc
     8461 gcatcacgcg ggattcgtct gttgcaggaa gaggaagaag ggttgccgct ggtaggtcgt
     8521 gtggctgccg gtgaaccagc tagcggcggc ggcggcagcg gcggcggcgg cagcaacggc
     8581 gcgattggtg gggatttgct gcttaacttt cccgacatgt ccgtgttgga acgtcagcgc
     8641 gcacatttga agtatcttaa ccccaccttc gactccccgt tggccgggtt ctttgcggac
     8701 tcatctatga ttacgggagg ggaaatggac agctacctct caacggccgg attgaatctt
     8761 ccgatgatgt atggagaaac cactgtagaa ggcgactcgc gactctcgat ttcgcctgaa
     8821 acgacgctgg gaacagggaa cttcaaggcg gcgaaattcg acacggagac aaaagattgc
     8881 aacgaagcgg cgaagaaaat gaccatgaat cgcgatgatc tggtagagga gggagaggag
     8941 gaaaagtcga agattactga acagaacaat gggtctacca aaagtatcaa aaagatgaag
     9001 cacaaagcta agaaagaaga gaacaatttc agcaatgaca gcagtaaagt cacaaaagaa
     9061 ctggagaaaa cggattacat tcacgtgagg gcgcgacgag ggcaggctac agattcacat
     9121 tcaattgcgg agagagtacg gagagagaaa atctcagaaa ggatgaagtt cctccaagac
     9181 cttgtgccag gttgtgacaa gatcacaggc aaagcaggaa tgctggatga gatcatcaac
     9241 tacgtccaat cgttgcaaag acaaattgag tttctctcga tgaaactggc catcgtgaat
     9301 cctagaccgg atttcgacat ggatgacatc tttgcgaaag aagtggcatc cactcccatg
     9361 acggttgtgc cctcaccgga gatggtcttg tctggttaca gccacgaaat ggtgcattcg
     9421 ggttattcaa gcgagatggt caattcggga taccttcacg tcaatcccat gcagcaggtg
     9481 aatacttcca gtggcggcgg cggcagcggc ggcggcggca gcggcttggc tactacactt
     9541 gaacgtattg agaagaactt tgtcattact gacccaagat tgccagataa tcccattata
     9601 ttcgcgtccg atagtttctt gcagttgaca gaatatagcc gtgaagaaat tttgggaaga
     9661 aactgcaggt ttctacaagg tcctgaaact gatcgcgcga cagtgagaaa aattagagat
     9721 gccatagata accaaacaga ggtcactgtt cagctgatta attatacaaa gagtggtaaa
     9781 aagttctgga acctctttca cttgcagcct atgcgagatc agaagggaga tgtccagtac
     9841 tttattgggg ttcagttgga tggaactgag catgtccgag atgctgccga gagagaggga
     9901 gtcatgctga ttaagaaaac tgcagaaaat attgacgagg ccgcaaagag actgcccgac
     9961 gccaacctgg cagccgcagc caagaagaaa aagctggact aatgagcggc cgcaactccc
    10021 acctgcaaca tgcgtgactg actgaggccg cgactctaga gtcgacctgc aggcatgcaa
    10081 gcttgatatc aagcttatcg ataatcaacc tctggattac aaaatttgtg aaagattgac
    10141 tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt
    10201 gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt
    10261 gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt
    10321 gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg
    10381 gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg
    10441 ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc
    10501 atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt
    10561 ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc
    10621 tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc
    10681 cgcctccccg catcgatacc gtcgacctcg agggaattaa t
//
