LOCUS       sequence-376880-       11483 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for constitutive expression of 
            LexA-CIB1-biLINuS-P2A-tagBFP..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11483)
  AUTHORS   Huang Z, Wu Y, Allen ME, Pan Y, Kyriakakis P, Lu S, Chang YJ, Wang 
            X, Chien S, Wang Y
  TITLE     Engineering light-controllable CAR T cells for cancer immunotherapy.
  JOURNAL   Sci Adv. 2020 Feb 19;6(8):eaay9209. doi: 10.1126/sciadv.aay9209. 
            eCollection 2020 Feb.
  PUBMED    32128416
REFERENCE   2  (bases 1 to 11483)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2020-02-19"; volume: "6(8):eaay9209. doi"; pages: " 
            10.1126/sciadv.aay9209. eCollection 2020 Feb"
COMMENT     Sequence Label: pHR-PGK-LexA-CIB1-biLINuS-P2A-tagBFP
FEATURES             Location/Qualifiers
     source          1..11483
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             104..337
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(362..380)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(363..380)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(670..689)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(903..922)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1022..1044
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1082..1100)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1168..1272
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1273..1341,1342..2133)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1491..1510)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2304..2892
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2793..2812
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3046..3063
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(3133..3153)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3138..3467
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3318..3453
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          4601..4666
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             4796..4816
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    5241..5375
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5278..5297)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5332..5351
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             5543..6176
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    6223..6348
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6845..7078
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7263..7307
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7592..7709
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        7769..8268
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(7804..7827)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     8201..8220
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             8302..8553
                     /codon_start=1
                     /gene="lexA (truncated)"
                     /product="DNA-binding domain of E. coli LexA"
                     /label=LexA BD
                     /note="binds the 16-bp palindromic sequence 
                     5'-CTGTATATATATACAG-3'"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAEE
                     HLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAA"
     CDS             10033..10089
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             10090..10791
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     misc_feature    10889..11477
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(10942..10962)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(11360..11371)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(join(11480..11483,1..13))
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(join(11481..11483,1..14))
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
ORIGIN
        1 accgtcgacc tcgagggaat taattcgagc tcggtacctt taagaccaat gacttacaag
       61 gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac
      121 tcccaacgaa gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc
      181 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
      241 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
      301 ctcagaccct tttagtcagt gtggaaaatc tctagcagca tctagaatta attccgtgta
      361 ttctatagtg tcacctaaat cgtatgtgta tgatacataa ggttatgtat taattgtagc
      421 cgcgttctaa cgacaatatg tacaagccta attgtgtagc atctggctta ctgaagcaga
      481 ccctatcatc tctctcgtaa actgccgtca gagtcggttt ggttggacga accttctgag
      541 tttctggtaa cgccgtcccg cacccggaaa tggtcagcga accaatcagc agggtcatcg
      601 ctagccagat cctctacgcc ggacgcatcg tggccggcat caccggcgcc acaggtgcgg
      661 ttgctggcgc ctatatcgcc gacatcaccg atggggaaga tcgggctcgc cacttcgggc
      721 tcatgagcgc ttgtttcggc gtgggtatgg tggcaggccc cgtggccggg ggactgttgg
      781 gcgccatctc cttgcatgca ccattccttg cggcggcggt gctcaacggc ctcaacctac
      841 tactgggctg cttcctaatg caggagtcgc ataagggaga gcgtcgaatg gtgcactctc
      901 agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct
      961 gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc
     1021 tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag
     1081 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg
     1141 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     1201 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     1261 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     1321 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     1381 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     1441 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     1501 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     1561 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     1621 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     1681 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     1741 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     1801 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     1861 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     1921 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     1981 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     2041 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     2101 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     2161 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     2221 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     2281 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     2341 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     2401 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     2461 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     2521 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     2581 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     2641 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     2701 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     2761 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     2821 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     2881 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     2941 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     3001 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
     3061 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
     3121 taatgcagct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag
     3181 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag
     3241 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc
     3301 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
     3361 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat
     3421 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag cttggacaca
     3481 agacaggctt gcgagatatg tttgagaata ccactttatc ccgcgtcagg gagaggcagt
     3541 gcgtaaaaag acgcggactc atgtgaaata ctggttttta gtgcgccaga tctctataat
     3601 ctcgcgcaac ctattttccc ctcgaacact ttttaagccg tagataaaca ggctgggaca
     3661 cttcacatga gcgaaaaata catcgtcacc tgggacatgt tgcagatcca tgcacgtaaa
     3721 ctcgcaagcc gactgatgcc ttctgaacaa tggaaaggca ttattgccgt aagccgtggc
     3781 ggtctgtacc gggtgcgtta ctggcgcgtg aactgggtat tcgtcatgtc gataccgttt
     3841 gtatttccag ctacgatcac gacaaccagc gcgagcttaa agtgctgaaa cgcgcagaag
     3901 gcgatggcga aggcttcatc gttattgatg acctggtgga taccggtggt actgcggttg
     3961 cgattcgtga aatgtatcca aaagcgcact ttgtcaccat cttcgcaaaa ccggctggtc
     4021 gtccgctggt tgatgactat gttgttgata tcccgcaaga tacctggatt gaacagccgt
     4081 gggatatggg cgtcgtattc gtcccgccaa tctccggtcg ctaatctttt caacgcctgg
     4141 cactgccggg cgttgttctt tttaacttca ggcgggttac aatagtttcc agtaagtatt
     4201 ctggaggctg catccatgac acaggcaaac ctgagcgaaa ccctgttcaa accccgcttt
     4261 aaacatcctg aaacctcgac gctagtccgc cgctttaatc acggcgcaca accgcctgtg
     4321 cagtcggccc ttgatggtaa aaccatccct cactggtatc gcatgattaa ccgtctgatg
     4381 tggatctggc gcggcattga cccacgcgaa atcctcgacg tccaggcacg tattgtgatg
     4441 agcgatgccg aacgtaccga cgatgattta tacgatacgg tgattggcta ccgtggcggc
     4501 aactggattt atgagtgggc cccggatctt tgtgaaggaa ccttacttct gtggtgtgac
     4561 ataattggac aaactaccta cagagattta aagctctaag gtaaatataa aatttttaag
     4621 tgtataatgt gttaaactac tgattctaat tgtttgtgta ttttagattc caacctatgg
     4681 aactgatgaa tgggagcagt ggtggaatgc ctttaatgag gaaaacctgt tttgctcaga
     4741 agaaatgcca tctagtgatg atgaggctac tgctgactct caacattcta ctcctccaaa
     4801 aaagaagaga aaggtagaag accccaagga ctttccttca gaattgctaa gttttttgag
     4861 tcatgctgtg tttagtaata gaactcttgc ttgctttgct atttacacca caaaggaaaa
     4921 agctgcactg ctatacaaga aaattatgga aaaatattct gtaaccttta taagtaggca
     4981 taacagttat aatcataaca tactgttttt tcttactcca cacaggcata gagtgtctgc
     5041 tattaataac tatgctcaaa aattgtgtac ctttagcttt ttaatttgta aaggggttaa
     5101 taaggaatat ttgatgtata gtgccttgac tagagatcat aatcagccat accacatttg
     5161 tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa
     5221 tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca
     5281 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     5341 ccaaactcat caatgtatct tatcatgtct ggatcaactg gataactcaa gctaaccaaa
     5401 atcatcccaa acttcccacc ccatacccta ttaccactgc caattacctg tggtttcatt
     5461 tactctaaac ctgtgattcc tctgaattat tttcatttta aagaaattgt atttgttaaa
     5521 tatgtactac aaacttagta gttggaaggg ctaattcact cccaaagaag acaagatatc
     5581 cttgatctgt ggatctacca cacacaaggc tacttccctg attagcagaa ctacacacca
     5641 gggccagggg tcagatatcc actgaccttt ggatggtgct acaagctagt accagttgag
     5701 ccagataagg tagaagaggc caataaagga gagaacacca gcttgttaca ccctgtgagc
     5761 ctgcatggga tggatgaccc ggagagagaa gtgttagagt ggaggtttga cagccgccta
     5821 gcatttcatc acgtggcccg agagctgcat ccggagtact tcaagaactg ctgatatcga
     5881 gcttgctaca agggactttc cgctggggac tttccaggga ggcgtggcct gggcgggact
     5941 ggggagtggc gagccctcag atcctgcata taagcagctg ctttttgcct gtactgggtc
     6001 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     6061 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     6121 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg
     6181 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact
     6241 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa
     6301 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg
     6361 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat
     6421 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc
     6481 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag
     6541 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat
     6601 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac
     6661 aaaagtaaga ccaccgcaca gcaagcggcc ggccgctgat cttcagacct ggaggaggag
     6721 atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat
     6781 taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg
     6841 gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt
     6901 caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca
     6961 atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca
     7021 agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg
     7081 ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt
     7141 ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag
     7201 aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag
     7261 aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta
     7321 acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag
     7381 gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac
     7441 cattatcgtt tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag
     7501 aagaagaagg tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac
     7561 ggtatcgcca aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt
     7621 acagtgcagg ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac
     7681 aaaaacaaat tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccag
     7741 tttggatcga taagcttgat atcgaattgg gtaggggagg cgcttttccc aaggcagtct
     7801 ggagcatgcg ctttagcagc cccgctgggc acttggcgct acacaagtgg cctctggcct
     7861 cgcacacatt ccacatccac cggtaggcgc caaccggctc cgttctttgg tggccccttc
     7921 gcgccacctt ctactcctcc cctagtcagg aagttccccc ccgccccgca gctcgcgtcg
     7981 tgcaggacgt gacaaatgga agtagcacgt ctcactagtc tcgtgcagat ggacagcacc
     8041 gctgagcaat ggaagcgggt aggcctttgg ggcagcggcc aatagcagct ttgctccttc
     8101 gctttctggg ctcagaggct gggaaggggt gggtccgggg gcgggctcag gggcgggctc
     8161 aggggcgggg cgggcgcccg aaggtcctcc ggaggcccgg cattctgcac gcttcaaaag
     8221 cgcacgtctg ccgcgctgtt ctcctcttcc tcatctccgg gcctttcgaa ttccgacctg
     8281 cagcccaagc ttaccgccac catgaaagcg ttaacggcca ggcaacaaga ggtgtttgat
     8341 ctcatccgtg atcacatcag ccagacaggt atgccgccga cgcgtgcgga aatcgcgcag
     8401 cgtttggggt tccgttcccc aaacgcggct gaagaacatc tgaaggcgct ggcacgcaaa
     8461 ggcgttattg aaattgtttc cggcgcatca cgcgggattc gtctgttgca ggaagaggaa
     8521 gaagggttgc cgctggtagg tcgtgtggct gccggtgaac cagctagcgg cggcggcggc
     8581 agcggcggcg gcggcagcaa cggcgcgatt ggtggggatt tgctgcttaa ctttcccgac
     8641 atgtccgtgt tggaacgtca gcgcgcacat ttgaagtatc ttaaccccac cttcgactcc
     8701 ccgttggccg ggttctttgc ggactcatct atgattacgg gaggggaaat ggacagctac
     8761 ctctcaacgg ccggattgaa tcttccgatg atgtatggag aaaccactgt agaaggcgac
     8821 tcgcgactct cgatttcgcc tgaaacgacg ctgggaacag ggaacttcaa ggcggcgaaa
     8881 ttcgacacgg agacaaaaga ttgcaacgaa gcggcgaaga aaatgaccat gaatcgcgat
     8941 gatctggtag aggagggaga ggaggaaaag tcgaagatta ctgaacagaa caatgggtct
     9001 accaaaagta tcaaaaagat gaagcacaaa gctaagaaag aagagaacaa tttcagcaat
     9061 gacagcagta aagtcacaaa agaactggag aaaacggatt acattcacgt gagggcgcga
     9121 cgagggcagg ctacagattc acattcaatt gcggagagag tacggagaga gaaaatctca
     9181 gaaaggatga agttcctcca agaccttgtg ccaggttgtg acaagatcac aggcaaagca
     9241 ggaatgctgg atgagatcat caactacgtc caatcgttgc aaagacaaat tgagtttctc
     9301 tcgatgaaac tggccatcgt gaatcctaga ccggatttcg acatggatga catctttgcg
     9361 aaagaagtgg catccactcc catgacggtt gtgccctcac cggagatggt cttgtctggt
     9421 tacagccacg aaatggtgca ttcgggttat tcaagcgaga tggtcaattc gggatacctt
     9481 cacgtcaatc ccatgcagca ggtgaatact tccagtggcg gcggcggcag cggcggcggc
     9541 ggcagcggct tggctactac acttgaacgt attgagaaga actttgtcat tactgaccca
     9601 agattgccag ataatcccat tatattcgcg tccgatagtt tcttgcagtt gacagaatat
     9661 agccgtgaag aaattttggg aagaaactgc aggtttctac aaggtcctga aactgatcgc
     9721 gcgacagtga gaaaaattag agatgccata gataaccaaa cagaggtcac tgttcagctg
     9781 attaattata caaagagtgg taaaaagttc tggaacctct ttcacttgca gcctatgcga
     9841 gatcagaagg gagatgtcca gtactttatt ggggttcagt tggatggaac tgagcatgtc
     9901 cgagatgctg ccgagagaga gggagtcatg ctgattaaga aaactgcaga aaatattgac
     9961 gaggccgcaa agagactgcc cgacgccaac ctggcagccg cagccaagaa gaaaaagctg
    10021 gacggaagcg gagctactaa cttcagcctg ctgaagcagg ctggagacgt ggaggagaac
    10081 cctggaccta tgagcgagct gattaaggag aacatgcaca tgaagctgta catggagggc
    10141 accgtggaca accatcactt caagtgcaca tccgagggcg aaggcaagcc ctacgagggc
    10201 acccagacca tgagaatcaa ggtggtcgag ggcggccctc tccccttcgc cttcgacatc
    10261 ctggctacta gcttcctcta cggcagcaag accttcatca accacaccca gggcatcccc
    10321 gacttcttca agcagtcctt ccctgagggc ttcacatggg agagagtcac cacatacgaa
    10381 gacgggggcg tgctgaccgc tacccaggac accagcctcc aggacggctg cctcatctac
    10441 aacgtcaaga tcagaggggt gaacttcaca tccaacggcc ctgtgatgca gaagaaaaca
    10501 ctcggctggg aggccttcac cgagacgctg taccccgctg acggcggcct ggaaggcaga
    10561 aacgacatgg ccctgaagct cgtgggcggg agccatctga tcgcaaacat caagaccaca
    10621 tatagatcca agaaacccgc taagaacctc aagatgcctg gcgtctacta tgtggactac
    10681 agactggaaa gaatcaagga ggccaacaac gagacctacg tcgagcagca cgaggtggca
    10741 gtggccagat actgcgacct ccctagcaaa ctggggcaca agcttaatta agcggccgca
    10801 actcccacct gcaacatgcg tgactgactg aggccgcgac tctagagtcg acctgcaggc
    10861 atgcaagctt gatatcaagc ttatcgataa tcaacctctg gattacaaaa tttgtgaaag
    10921 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat
    10981 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc
    11041 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg
    11101 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct
    11161 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct
    11221 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg
    11281 gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac
    11341 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct
    11401 gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct
    11461 ttgggccgcc tccccgcatc gat
//
