LOCUS       sequence-376882-        4399 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mCherry under T7 promoter control.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4399)
  AUTHORS   Baumschlager A, Aoki SK, Khammash M
  TITLE     Dynamic Blue Light-Inducible T7 RNA Polymerases (Opto-T7RNAPs) for 
            Precise Spatiotemporal Gene Expression Control.
  JOURNAL   ACS Synth Biol. 2017 Nov 17;6(11):2157-2167. doi: 
            10.1021/acssynbio.7b00169. Epub 2017 Oct 18.
  PUBMED    29045151
REFERENCE   2  (bases 1 to 4399)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.7b00169"; journalName: "ACS Synth Biol"; date: 
            "2017-11-17- 17"; volume: "6"; issue: "11"; pages: "2157-2167"
COMMENT     Sequence Label: pAB213
FEATURES             Location/Qualifiers
     source          1..4399
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     160..181
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(1150..1167)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1284..1829)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(1364..1383)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1905..1924
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     2025..2044
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2025..2043
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2874..2891)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      3044..3130
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      3222..3249
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        3268..3359
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3360..3428,3429..4220)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3578..3597)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(4262..4399,1..318)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4349..4368)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
       61 accccaaaaa acttgatttg ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
      121 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
      181 gaacaacact caaccctatc tcgggctatt cttttgattt ataagggatt ttgccgattt
      241 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa
      301 tattaacgtt tacaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
      361 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
      421 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
      481 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
      541 actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc
      601 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
      661 gtcaggcatt tgagaagcac acggtcacac tgcttccggt agtcaataaa ccggtaaacc
      721 agcaatagac ataagcggct atttaacgac cctgccctga accgacgacc gggtcgaatt
      781 tgctttcgaa tttctgccat tcatccgctt attatcactt attcaggcgt agcaaccagg
      841 cgtttaaggg caccaataac tgccttaaaa aaattacgcc ccgccctgcc actcatcgca
      901 gtactgttgt aattcattaa gcattctgcc gacatggaag ccatcacaaa cggcatgatg
      961 aacctgaatc gccagcggca tcagcacctt gtcgccttgc gtataatatt tgcccatggc
     1021 tagcggagtg tatactggct tactatgttg gcactgatga gggtgtcagt gaagtgcttc
     1081 atgtggcagg agaaaaaagg ctgcaccggt gcgtcagcag aatatgtgat acaggatata
     1141 ttccgcttcc tcgctcactg actcgctacg ctcggtcgtt cgactgcggc gagcggaaat
     1201 ggcttacgaa cggggcggag atttcctgga agatgccagg aagatactta acagggaagt
     1261 gagagggccg cggcaaagcc gtttttccat aggctccgcc cccctgacaa gcatcacgaa
     1321 atctgacgct caaatcagtg gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     1381 ccccctggcg gctccctcgt gcgctctcct gttcctgcct ttcggtttac cggtgtcatt
     1441 ccgctgttat ggccgcgttt gtctcattcc acgcctgaca ctcagttccg ggtaggcagt
     1501 tcgctccaag ctggactgta tgcacgaacc ccccgttcag tccgaccgct gcgccttatc
     1561 cggtaactat cgtcttgagt ccaacccgga aagacatgca aaagcaccac tggcagcagc
     1621 cactggtaat tgatttagag gagttagtct tgaagtcatg cgccggttaa ggctaaactg
     1681 aaaggacaag ttttggtgac tgcgctcctc caagccagtt acctcggttc aaagagttgg
     1741 tagctcagag aaccttcgaa aaaccgccct gcaaggcggt tttttcgttt tcagagcaag
     1801 agattacgcg cagaccaaaa cgatctcaag aagatcatct tattaatcag ataaaatatt
     1861 tctaggatca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata
     1921 taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag
     1981 aggatctgct catgtttgac agcttatcat cgatgcatcc taggtaatac gactcactat
     2041 agggagagga tcctgtcaat ttccgcgata gaggaggtaa agcatatggt ttcaaaaggc
     2101 gaagaagaca acatggcgat tatcaaggaa tttatgcgtt tcaaggtcca catggaaggc
     2161 agcgtcaatg gtcacgaatt tgaaattgaa ggcgaaggtg aaggccgtcc gtatgaaggc
     2221 acccagacgg caaaactgaa ggtcaccaaa ggcggtccgc tgccgtttgc ttgggatatt
     2281 ctgtcaccgc aattcatgta tggttcgaaa gcgtacgtta agcatccggc cgatatcccg
     2341 gactatctga aactgtcctt tccggaaggc ttcaaatggg aacgtgttat gaacttcgaa
     2401 gatggcggtg tggttaccgt cacgcaggat agctctctgc aagacggtga atttatttat
     2461 aaagtgaagc tgcgcggcac caatttcccg agcgatggtc cggttatgca gaaaaagacg
     2521 atgggctggg aagcgagttc cgaacgtatg tacccggaag acggtgccct gaaaggcgaa
     2581 atcaagcagc gcctgaaact gaaggatggc ggtcactatg acgcagaagt gaaaaccacg
     2641 tacaaggcta aaaagccggt ccaactgccg ggtgcataca acgtgaacat caagctggat
     2701 atcaccagcc ataacgaaga ctatacgatc gttgaacagt acgaacgtgc agaaggccgc
     2761 cactctaccg gcggtatgga tgaactgtac aaataaggta cccggggatc ctctagagtc
     2821 gacctgcagg catgcaagct tggctgtttt ggcggatgag agaagatttt cagcctgata
     2881 cagattaaat cagaacgcag aagcggtctg ataaaacaga atttgcctgg cggcagtagc
     2941 gcggtggtcc cacctgaccc catgccgaac tcagaagtga aacgccgtag cgccgatggt
     3001 agtgtggggt ctccccatgc gagagtaggg aactgccagg catcaaataa aacgaaaggc
     3061 tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctcctgag
     3121 taggacaaat ccgccgggag cggatttgaa cgttgcgaag caacggcccg gagggtggcg
     3181 ggcaggacgc ccgccataaa ctgccaggca tcaaattaag cagaaggcca tcctgacgga
     3241 tggccttttt gcgtttctac aaactctttt gtttattttt ctaaatacat tcaaatatgt
     3301 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     3361 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     3421 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     3481 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     3541 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     3601 gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     3661 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     3721 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     3781 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     3841 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     3901 ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     3961 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     4021 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     4081 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     4141 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     4201 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     4261 tacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     4321 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     4381 acgttcgccg gctttcccc
//
