LOCUS       sequence-376895-        2640 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Insert: intronic Tau authentic Alu and repeat elements. Introns are 
            flanked by the tau cDNA exons 10-12 with FTLD-Tau V337M mutant. 
            Expresses tau circular RNA 12-->10 VM with 3X flag tag in exon 10..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2640)
  AUTHORS   Welden JR, Margvelani G, Arizaca Maquera KA, Gudlavalleti B, Miranda
            Sardon SC, Campos AR, Robil N, Lee DC, Hernandez AG, Wang WX, Di J, 
            de la Grange P, Nelson PT, Stamm S
  TITLE     RNA editing of microtubule-associated protein tau circular RNAs 
            promotes their translation and tau tangle formation.
  JOURNAL   Nucleic Acids Res. 2022 Dec 9;50(22):12979-12996. doi: 
            10.1093/nar/gkac1129.
  PUBMED    36533443
REFERENCE   2  (bases 1 to 2640)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nucleic Acids Res"; date: "2022-12-9- 9"; volume: "50"; issue: 
            "22"; pages: "12979-12996"
COMMENT     Sequence Label: pcDNA3.1_Tau Authentic Alu 10-12V337M
FEATURES             Location/Qualifiers
     source          1..2640
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1345..1410
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             2292..2303
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 ccaggctgat ggggcctcca gggcagtggt ttccacccag cattctcaaa agagccactg
       61 aggtctcagt gccattttca agatttcgga agcggcctgg gcacggctgg tccttcactg
      121 ggatcaccac ttggcaatta tttacacctg agacgaataa aaaccagagt gctgagatta
      181 caggcatggt ggcttacgct tgtaatcggc tttgggaagc cgaggtgggc tgattgcttg
      241 agcccaggag tttcaaacta tcctggacaa catagcatga cctcgtctct acaaaaaata
      301 caaaaaattt gccaggtgtg gtggcatgtg cctgtggtcc cagctacttg ggaggctgaa
      361 gtaggagaat cccctgagcc ctgggaagtc gaggctgcac tgagccgtga tgatgtcact
      421 gcactccagc ctgggtgaca aagtgagacc ctatctcaca aagaaaaaaa acaaaacaaa
      481 aaacccaaag cacactgttt ccactgtttc cagagttcct gagaggaaag gtcaccgggt
      541 gaggaagacg ttctcactga tctggcagag aaaatgtcca gtttttccaa ctccctaaac
      601 catggttttc tatttcacag cccccccttg cagggacaga gccctcgtac agaagggaca
      661 ccccacattt gtcttcccca caaagcggcc tgtgtcctgc ctacggggtc agggcttctc
      721 aaacctggct gtgtgtcaga atcaccaggg gaacttttca aaactagaga gactgaagcc
      781 agactcctag attctaattc taggtcaggg ctaggggctg agattgtaaa aatccacagg
      841 tgattctgat gcccggcagg cttgagaaca gccgcaggga gttctctggg aatgtgccgg
      901 tgggtctagc caggtgtgag tggagatgcc ggggaacttc ctattactca ctcgtcagtg
      961 tggccgaaca catttttcac ttgacctcag gctggtgaac gctcccctct ggggttcagg
     1021 cctcacgatg ccatcctttt gtgaagtgag gacctgcaat cccagcttcg taaagcccgc
     1081 tggaaatcac tcacacttct gggatgcctt cagagcagcc ctctatccct tcagctcccc
     1141 tgggatgtga ctcgacctcc cgtcactccc cagactgcct ctgccaagtc cgaaagtgga
     1201 ggcatccttg cgagcaagta ggcgggtcca gggtggcgca tgtcactcat cgaaagtgga
     1261 ggcgtccttg cgagcaagca ggcgggtcca gggtggcgtg tcactcatcc ttttttctgg
     1321 ctaccaaagg tgcagataat taatgactac aaagaccatg acggtgatta taaagatcat
     1381 gacatcgatt acaaggatga cgatgacaag aagaagctgg atcttagcaa cgtccagtcc
     1441 aagtgtggct caaaggataa tatcaaacac gtcccgggag gcggcagtgt gcaaatagtc
     1501 tacaaaccag ttgacctgag caaggtgacc tccaagtgtg gctcattagg caacatccat
     1561 cataaaccag gaggtggcca gatggaagta aaatctgaga agcttgactt caaggacaga
     1621 gtccagtcga agattgggtc cctggacaat atcacccacg tccctggcgg aggaaataaa
     1681 aaggtaaagg gggtagggtg ggttggatgc tgcccttggg tatatgggca ttaatcaagt
     1741 tgagtggaca aaggctggtc cagttcccag aggaggaaaa cagaggcttc tgtgttgact
     1801 ggctggatgt gggccctcag cagcatccag tgggtctcca ctgcctgtct caatcacctg
     1861 gagctttagc acgtttcaca cctgggcccc aacctggaga ggctgaccaa tgggtctcag
     1921 gggcagctcg gttgctggag tttttgtttt tatttatttt tatgtattta aggcagggtc
     1981 tctgtattag tccattctca cactgctaat aaagacatac ccaagactgg gtaatttata
     2041 aaggaaagag gtttaatgga ctcacagttc catatggctg gggaggcctc aaaatcatgg
     2101 cggaaggcaa aggagaagca aaggcatgtc ttacatggca acaggcaaga gagcgtgtgc
     2161 aggggaactc ccatttataa aaccatcaga cctcatgaga tttattcact atcatgagaa
     2221 cagcatggga aagacccgcc cccatgattc agttacctcc cactgggtcc ctcccatgac
     2281 acatggaatt atgggagcta caattcaaga tgagatttgg gtggggacac agccaaacca
     2341 tatcagtctc cctctgtcat ccaggctgga gtgcactggc atgatctcgg ctcactgcag
     2401 cctctacctc cctgggtcag gtgatcttcc cacctcagcc tcccaggtag ctggaactac
     2461 aggtacctgc cactatgcct ggctaaatat tttgtatttc ctgtggagac gaggttttgc
     2521 cacgttgccc aggctggtct tgaactcctg aggtcaagca atatgcccac ctcggcctcc
     2581 caaggtgctg ggattacagg tgtgagccac agtgctcggc ctaagtcact gcagttttta
//
