LOCUS       sequence-376896-        8092 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express in E. coli human Naa80 delta (actin's N-terminal acetyl 
            transferase) with N-terminal intein-chitin binding domain tag and 
            6xHis tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8092)
  AUTHORS   Ceron RH, Carman PJ, Rebowski G, Boczkowska M, Heuckeroth RO, 
            Dominguez R
  TITLE     A solution to the long-standing problem of actin expression and 
            purification.
  JOURNAL   Proc Natl Acad Sci U S A. 2022 Oct 11;119(41):e2209150119. doi: 
            10.1073/pnas.2209150119. Epub 2022 Oct 5.
  PUBMED    36197995
REFERENCE   2  (bases 1 to 8092)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2022-10-11"; volume: "119(41):e2209150119. 
            doi"; pages: " 10.1073/pnas.2209150119. Epub 2022 Oct 5"
COMMENT     Sequence Label: His_Naa80d in pTYB12
FEATURES             Location/Qualifiers
     source          1..8092
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      1..28
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        47..138
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(139..207,208..999)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(357..376)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      complement(1041..1554)
                     /direction=LEFT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1236..1257)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1448..1467
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     rep_origin      1665..2253
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2154..2173
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2407..2424
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(2446..2465)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2606..2628
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(2622..2813)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(3370..4452)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     4414..4433
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(4453..4530)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     4736..4755
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     4839..4858
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4839..4857
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    4858..4882
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             4897..4919
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             4972..5790
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae VMA1"
                     /product="5' region of the intein from the yeast Vma1 
                     subunit of the vacuolar ATPase (Chong et al., 1998)"
                     /label=Sce VMA intein 5' region
                     /translation="CFAKGTNVLMADGSIECIENIEVGNKVMGKDGRPREVIKLPRGRE
                     TMYSVVQKSQHRAHKSDSSREVPELLKFTCNATHELVVRTPRSVRRLSRTIKGVEYFEV
                     ITFEMGQKKAPDGRIVELVKEVSKSYPISEGPERANELVESYRKASNKAYFEWTIEARD
                     LSLLGSHVRKATYQTYAPILYENDHFFDYMQKSKFHLTIEGPKVLAYLLGLWIGDGLSD
                     RATFSVDSRDTSLMERVTEYAEKLNLCAEYKDRKEPQVAKTVNLYSKVVRG"
     CDS             5797..5949
                     /codon_start=1
                     /gene="Bacillus circulans chiA"
                     /product="chitin binding domain from chitinase A1 (Watanabe
                     et al., 1994)"
                     /label=CBD
                     /translation="TNPGVSAWQVNTAYTAGQLVTYNGKTYKCLQPHTSLAGWEPSNVP
                     ALWQLQ"
     CDS             5953..6501
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae VMA1"
                     /product="modified 3' region of the intein from the yeast 
                     Vma1 subunit of the vacuolar ATPase (Chong et al., 1998)"
                     /label=Sce VMA intein 3' region
                     /note="C-terminal cleavage of this intein can be induced by
                     thiols."
                     /translation="GHGGIRNNLNTENPLWDAIVGLGFLKDGVKNIPSFLSTDNIGTRE
                     TFLAGLIDSDGYVTDEHGIKATIKTIHTSVRDGLVSLARSLGLVVSVNAEPAKVDMNVT
                     KHKISYAIYMSGGDVLLNVLSKCAGSKKFRPAPAAAFARECRGFYFELQELKEDDYYGI
                     TLSDDSDHQFLLGSQVVVQN"
     CDS             6508..6525
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(7301..7319)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      7315..7362
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(7550..7567)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7550..7566)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7559..7581)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(7745..7762)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      7915..8001
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 agaaggccat cctgacggat ggcctttttg cgtttctaca aactcttttg tttatttttc
       61 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
      121 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
      181 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
      241 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
      301 cttgagagtt ttcgccccga agaacgtttc ccaatgatga gcacttttaa agttctgcta
      361 tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac
      421 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
      481 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
      541 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
      601 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
      661 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
      721 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
      781 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
      841 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
      901 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
      961 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
     1021 tatatacttt agattgattt accccggttg ataatcagaa aagccccaaa aacaggaaga
     1081 ttgtataagc aaatatttaa attgtaaacg ttaatatttt gttaaaattc gcgttaaatt
     1141 tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat
     1201 caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat
     1261 taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac
     1321 tacgtgaacc atcacccaaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc
     1381 ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga
     1441 gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca
     1501 cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtaaaagg
     1561 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     1621 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     1681 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     1741 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     1801 ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     1861 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     1921 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     1981 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     2041 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     2101 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     2161 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     2221 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     2281 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     2341 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     2401 gagcgcagcg agtcagtgag cgaggaagct atggtgcact ctcagtacaa tctgctctga
     2461 tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg
     2521 ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc
     2581 gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca
     2641 tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg cagcgattca
     2701 cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc
     2761 tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc
     2821 tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg
     2881 ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa
     2941 caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagccg
     3001 aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc
     3061 tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt
     3121 ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg
     3181 aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc
     3241 ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag
     3301 gctctcaagg gcatcggtcg agatcccggt gcctaatgag tgagctaact tacattaatt
     3361 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
     3421 atcggccaac gcgcggggag aggcggtttg cgtattgggc gccagggtgg tttttctttt
     3481 caccagtgag acgggcaaca gctgattgcc cttcaccgcc tggccctgag agagttgcag
     3541 caagcggtcc acgctggttt gccccagcag gcgaaaatcc tgtttgatgg tggttaacgg
     3601 cgggatataa catgagctgt cttcggtatc gtcgtatccc actaccgaga tatccgcacc
     3661 aacgcgcagc ccggactcgg taatggcgcg cattgcgccc agcgccatct gatcgttggc
     3721 aaccagcatc gcagtgggaa cgatgccctc attcagcatt tgcatggttt gttgaaaacc
     3781 ggacatggca ctccagtcgc cttcccgttc cgctatcggc tgaatttgat tgcgagtgag
     3841 atatttatgc cagccagcca gacgcagacg cgccgagaca gaacttaatg ggcccgctaa
     3901 cagcgcgatt tgctggtgac ccaatgcgac cagatgctcc acgcccagtc gcgtaccgtc
     3961 ttcatgggag aaaataatac tgttgatggg tgtctggtca gagacatcaa gaaataacgc
     4021 cggaacatta gtgcaggcag cttccacagc aatggcatcc tggtcatcca gcggatagtt
     4081 aatgatcagc ccactgacgc gttgcgcgag aagattgtgc accgccgctt tacaggcttc
     4141 gacgccgctt cgttctacca tcgacaccac cacgctggca cccagttgat cggcgcgaga
     4201 tttaatcgcc gcgacaattt gcgacggcgc gtgcagggcc agactggagg tggcaacgcc
     4261 aatcagcaac gactgtttgc ccgccagttg ttgtgccacg cggttgggaa tgtaattcag
     4321 ctccgccatc gccgcttcca ctttttcccg cgttttcgca gaaacgtggc tggcctggtt
     4381 caccacgcgg gaaacggtct gataagagac accggcatac tctgcgacat cgtataacgt
     4441 tactggtttc acattcacca ccctgaattg actctcttcc gggcgctatc atgccatacc
     4501 gcgaaaggtt ttgcgccatt cgatggtgtc cgggatctcg acgctctccc ttatgcgact
     4561 cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga
     4621 atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac
     4681 ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga
     4741 tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc
     4801 gtccggcgta gaggatcgag atctcgatcc cgcgaaatta atacgactca ctatagggga
     4861 attgtgagcg gataacaatt cccctctaga aataattttg tttaacttta agaaggagat
     4921 atacttatga aaatcgaaga aggtaaactg gtaatcggtt ccctggaggg ttgctttgcc
     4981 aagggtacca atgttttaat ggcggatggg tctattgaat gtattgaaaa cattgaggtt
     5041 ggtaataagg tcatgggtaa agatggcaga cctcgtgagg taattaaatt gcccagagga
     5101 agagaaacta tgtacagcgt cgtgcagaaa agtcagcaca gagcccacaa aagtgactca
     5161 agtcgtgaag tgccagaatt actcaagttt acgtgtaatg cgacccatga gttggttgtt
     5221 agaacacctc gtagtgtccg ccgtttgtct cgtaccatta agggtgtcga atattttgaa
     5281 gttattactt ttgagatggg ccaaaagaaa gcccccgacg gtagaattgt tgagcttgtc
     5341 aaggaagttt caaagagcta cccaatatct gaggggcctg agagagccaa cgaattagta
     5401 gaatcctata gaaaggcttc aaataaagct tattttgagt ggactattga ggccagagat
     5461 ctttctctgt tgggttccca tgttcgtaaa gctacctacc agacttacgc tccaattctt
     5521 tatgagaatg accacttttt cgactacatg caaaaaagta agtttcatct caccattgaa
     5581 ggtccaaaag tacttgctta tttacttggt ttatggattg gtgatggatt gtctgacagg
     5641 gcaacttttt cggttgattc cagagatact tctttgatgg aacgtgttac tgaatatgct
     5701 gaaaagttga atttgtgcgc cgagtataag gacagaaaag aaccacaagt tgccaaaact
     5761 gttaatttgt actctaaagt tgtcagaggt gctagcacaa atcctggtgt atccgcttgg
     5821 caggtcaaca cagcttatac tgcgggacaa ttggtcacat ataacggcaa gacgtataaa
     5881 tgtttgcagc cccacacctc cttggcagga tgggaaccat ccaacgttcc tgccttgtgg
     5941 cagcttcaag gtggccatgg tggtattcgc aataatctta atactgagaa tccattatgg
     6001 gacgctattg ttggcttagg attcttgaag gacggtgtca aaaatattcc ttctttcttg
     6061 tctacggaca atatcggtac tcgtgaaaca tttcttgctg gtctaattga ttctgatggc
     6121 tatgttactg atgagcatgg tattaaagca acaataaaga caattcatac ttctgtcaga
     6181 gatggtttgg tttcccttgc tcgttcttta ggcttagtag tctcggttaa cgcagaacct
     6241 gctaaggttg acatgaatgt caccaaacat aaaattagtt atgctattta tatgtctggt
     6301 ggagatgttt tgcttaacgt tctttcgaag tgtgccggct ctaaaaaatt caggcctgct
     6361 cccgccgctg cttttgcacg tgagtgccgc ggattttatt tcgagttaca agaattgaag
     6421 gaagacgatt attatgggat tactttatct gatgattctg atcatcagtt tttgcttgga
     6481 tcccaggttg ttgtacagaa tgctggtcat caccatcatc accatatgag cctggctgag
     6541 ttgaccctgg agcctgtgca ccgccgaccc gagctcctgg atgcttgtgc tgacctcatc
     6601 aatgatcagt ggccccgcag ccgcacctcc cgcctgcact ccctgggcca gtcctcagat
     6661 gccttccccc tctgcctgat gctgctaagc ccccacccca cacttgaagc agcacccgtt
     6721 gtggtgggcc atgcccgcct gtcacgggtg ctgaaccagc cccagagcct cttagtggag
     6781 acagtggtgg tggcccgggc cctgaggggc cgtggctttg gccgccgcct catggagggc
     6841 ctggaggtct ttgctcgggc ccggggcttc cgcaagctgc atctcaccac ccatgaccag
     6901 gtgcacttct atacccacct gggctaccag ctgggtgagc ctgtgcaggg cctggtcttc
     6961 accagcagac ggctgcctgc caccctgctt aatgccttcc ccacagcccc ctctccccgg
     7021 ccacccagga aggccccaaa cctgactgcc caagctgccc caaggggtcc caagggacct
     7081 ccattgccac caccccctcc cctacctgag tgcctgacca tctcaccccc agttccatca
     7141 gggccccctt caaaaagcct gctggagaca caatatcaaa atgtgagggg gcgccccata
     7201 ttctggatgg aaaaagacat ctgactcgag cccgggtgac tgcaggaagg ggatccggct
     7261 gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca
     7321 taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata
     7381 tccggatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat
     7441 ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct
     7501 gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttggca ctggccgtcg
     7561 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac
     7621 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
     7681 agttgcgcag cctgaatggc gaatggcagc ttggctgttt tggcggatga gataagattt
     7741 tcagcctgat acagattaaa tcagaacgca gaagcggtct gataaaacag aatttgcctg
     7801 gcggcagtag cgcggtggtc ccacctgacc ccatgccgaa ctcagaagtg aaacgccgta
     7861 gcgccgatgg tagtgtgggg tctccccatg cgagagtagg gaactgccag gcatcaaata
     7921 aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac
     7981 gctctcctga gtaggacaaa tccgccggga gcggatttga acgttgcgaa gcaacggccc
     8041 ggagggtggc gggcaggacg cccgccataa actgccaggc atcaaattaa gc
//
