LOCUS       sequence-376898-        9677 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For use in RBP tethering screen.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9677)
  AUTHORS   Luo EC, Nathanson JL, Tan FE, Schwartz JL, Schmok JC, Shankar A, 
            Markmiller S, Yee BA, Sathe S, Pratt GA, Scaletta DB, Ha Y, Hill DE,
            Aigner S, Yeo GW
  TITLE     Large-scale tethered function assays identify factors that regulate 
            mRNA stability and translation.
  JOURNAL   Nat Struct Mol Biol. 2020 Oct;27(10):989-1000. doi: 
            10.1038/s41594-020-0477-6. Epub 2020 Aug 17.
  PUBMED    32807991
REFERENCE   2  (bases 1 to 9677)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41594-020-0477-6"; journalName: "Nat Struct Mol Biol"; 
            date: "2020-10"; volume: "27"; issue: "10"; pages: "989-1000"
COMMENT     Sequence Label: KIF1C
FEATURES             Location/Qualifiers
     source          1..9677
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     primer_bind     1196..1215
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1196..1214
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1293..1317
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      1318..1327
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      3547..3556
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(4634..4658)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4783..4824
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(5279..5296)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5285..5509
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5642..5661)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    5793..5840
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             5848..6870
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             5848..6870
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    7000..7121
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7037..7056)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7091..7110
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7170..7186)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7170..7186)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7183..7205)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7194..7210
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7218..7224,7225..7242,7243..7248))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7263..7284
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7401..7418)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7572..8160)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7652..7671)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8331..9122,9123..9191))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8954..8973
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9192..9296)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9371..9390)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gaattagctt ggtactaata
     1201 cgactcacta tagggagacc caagctggct aggtaagctt ggtaccgagc tcggatccac
     1261 tagtccagtg tggtggaatt ctgcagatat caacaagttt gtacaaaaaa gcaggctgcc
     1321 accatggctg gtgcctcggt gaaagtggca gtgagggttc ggccctttaa cgcccgtgag
     1381 accagccagg atgccaagtg tgtggtcagc atgcagggca acaccacctc catcatcaat
     1441 cctaaacaga gcaaggatgc ccccaaaagc ttcacctttg actactccta ctggtcacac
     1501 acttcgacgg aggaccccca gtttgcatct cagcagcaag tgtatcggga cattggagaa
     1561 gagatgctgc tccacgcctt tgaaggctac aacgtgtgca tctttgccta tgggcagacc
     1621 ggggctggga aatcctatac catgatgggg cgacaggagc cagggcagca gggcatcgtg
     1681 ccccagctct gtgaggacct cttctctcgc gttagtgaga accagagtgc tcagctatcc
     1741 tactctgtgg aggtgagcta tatggagatc tactgtgagc gggtacgaga cctcttgaac
     1801 cccaagagtc ggggttctct gcgggtccgg gagcacccca tcctgggccc gtacgtgcag
     1861 gacctgtcca aattggctgt gacctcctac gcagacattg ctgacctcat ggactgtgga
     1921 aataaagcac ggactgtggc tgccaccaac atgaatgaga ccagcagccg ttcccatgcc
     1981 gtctttacca tcgtcttcac acagcgctgc catgaccagc tcacggggct ggactcggag
     2041 aaggtcagta agatcagttt ggtggacctt gctgggagtg agcgagccga ctcctcaggg
     2101 gcccggggca tgcgcctgaa ggaaggagcc aacatcaata agtccctgac tacactaggg
     2161 aaagtgatct cggcccttgc agatatgcaa tcaaagaagc gaaagtcgga ttttatcccc
     2221 tacagggact ctgtgctcac ctggctgctc aaggaaaatt tgggggggaa ctcacgcaca
     2281 gccatgattg cagccctgag ccctgctgac atcaattacg aggagactct cagcaccctc
     2341 aggtatgctg accgcaccaa gcaaatccgc tgcaatgcca tcatcaacga ggaccctaat
     2401 gcccggctga ttagagagct gcaggaggaa gtagcccggc tgcgggaact gctgatggct
     2461 cagggactgt cagcctctgc tctggaaggc ctgaagacgg aagaagggag tgtcagaggc
     2521 gccctgccag ctgtgtcatc tcccccagct ccagtttcac cctcatcacc caccacacat
     2581 aatggggagc tggagccgtc attctccccc aacacggagt cccagattgg gcctgaggaa
     2641 gccatggaga ggctgcagga gacagagaag attatagctg agctgaacga gacatgggag
     2701 gagaagctac gcaagacaga agccctgagg atggagagag aagcattgct ggctgagatg
     2761 ggggtggccg tccgggagga tgggggaact gtgggcgtct tctctccaaa gaagactccc
     2821 cacctggtga acctgaacga agaccctctg atgtctgagt gtctgctcta ccacatcaaa
     2881 gatggcgtca ccagggtcgg ccaagtagat atggacatca agctgaccgg acagttcatt
     2941 cgggagcaac actgtctgtt ccggagcatc ccccagccag atggagaagt ggtggtcact
     3001 ctggagcctt gtgaaggagc tgagacatat gtgaatggga agcttgtgac ggagccgctg
     3061 gtgctgaagt cagggaatag gattgtgatg ggcaagaacc acgttttccg cttcaaccac
     3121 ccggagcagg caaggctgga acgggaacga ggggtccccc cacccccagg accgccctct
     3181 gagccagtcg actggaactt tgcccagaag gaactgctgg agcagcaagg catcgacata
     3241 aagctggaaa tggagaagag gctgcaggat ctggagaatc agtaccggaa agaaaaggaa
     3301 gaagccgatc ttctgctgga gcagcagcga ctgtatgcag actcggacag cggggatgac
     3361 tctgacaagc gctcttgtga agagagctgg aggctcatct cctccttgcg ggagcagctg
     3421 ccgcccacca cggtccagac cattgtcaaa cgctgtggtc tgcccagcag tggcaagcgc
     3481 agggcccctc gcagggttta tcagatcccc cagcgacgca ggctgcaggg caaagacccc
     3541 cgctgggcca ccatggctga cctgaagatg caggcggtga aggagatctg ctacgaggtg
     3601 gccctggctg acttccgcca cgggcgggct gagattgagg ccctggccgc cctcaagatg
     3661 cgggagctgt gtcgcaccta tggcaagcca gacggccccg gagacgcctg gagggctgtg
     3721 gcccgggatg tctgggacac tgtaggcgag gaggaaggag gtggagctgg cagtggtggt
     3781 ggcagtgagg agggagcccg aggggcggag gtggaggacc tccgggccca catcgacaag
     3841 ctgacgggga ttctgcagga ggtgaagctg cagaacagca gcaaggaccg ggagctgcag
     3901 gccctgcggg accgcatgct ccgcatggag agggtcatcc ccctggccca ggatcatgag
     3961 gatgagaatg aagaaggtgg tgaggtcccc tgggccccgc ctgaaggatc agaggcagca
     4021 gaggaggcag cccccagtga ccgcatgccg tcagcccggc ccccctcgcc accactgtca
     4081 agctgggagc gggtgtcacg gctcatggag gaggaccctg ccttccgtcg tggtcgtctt
     4141 cgctggctca agcaggagca gctacggctg cagggactgc agggctctgg gggccggggc
     4201 ggggggctgc gcaggccccc agcccgcttt gtgccccctc acgactgcaa gctacgcttc
     4261 cccttcaaga gcaaccccca gcaccgggag tcttggccag ggatggggag cggggaggct
     4321 ccaactccgc tccaaccccc tgaggaggtc actccccatc cagccacccc tgcccgccgg
     4381 cctccgagtc cccgaaggtc ccaccatccc cgcaggaact ccctggatgg agggggccga
     4441 tcccggggag cgggttctgc acagcctgaa ccccagcact tccagcccaa aaagcacaac
     4501 tcttatcccc agccacccca accctaccca gcccagcggc ccccagggcc ccgctacccc
     4561 ccatacacta ctcccccacg aatgagacgg cagcgttctg cccctgacct caaggagagt
     4621 ggggcagctg tggacccagc tttcttgtac aaagtggttg atatccagca cagtggcggc
     4681 cgctcgagtc tagagggccc gcggttcgaa accagcggag gcggcggaag cgggggagga
     4741 ggcagcggcg gaggcggcag cgggggcggc ggaagcgcta ccggtaagcc tatccctaac
     4801 cctctcctcg gtctcgattc tacgcgtacc ggtatggctt ctaactttac tcagttcgtt
     4861 ctcgtcgaca atggcggaac tggcgacgtg actgtcgccc caagcaactt cgctaacggg
     4921 gtcgctgaat ggatcagctc taactcgcga tcacaggctt acaaagtaac ctgtagcgtt
     4981 cgtcagagct ctgcgcagaa tcgcaaatac accatcaaag tcgaggtgcc taaagtggca
     5041 acccagactg ttggtggtga agagcttcct gtagccggat ggagatctta cttaaatatg
     5101 gaactaacca ttccaatttt cgccacgaat tccgactgcg agcttattgt taaggcaatg
     5161 caaggtctcc taaaagatgg aaacccgatt ccctcggcca tcgcggccaa ctccggcatc
     5221 tacggaggtg gaggtggatc tcgtacgggt tagtaatgag tttaaacccg ctgatcagcc
     5281 tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg
     5341 accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat
     5401 tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag
     5461 gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg
     5521 gaaagaacca gctggggctc tagggggtat ccccacgcgc cctgtagcgg cgcattaagc
     5581 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     5641 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     5701 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     5761 aaacttgatt agggtgatgg ttcacgtacc tagaagttcc tattccgaag ttcctattct
     5821 ctagaaagta taggaacttc cttggccaaa aagcctgaac tcaccgcgac gtctgtcgag
     5881 aagtttctga tcgaaaagtt cgacagcgtc tccgacctga tgcagctctc ggagggcgaa
     5941 gaatctcgtg ctttcagctt cgatgtagga gggcgtggat atgtcctgcg ggtaaatagc
     6001 tgcgccgatg gtttctacaa agatcgttat gtttatcggc actttgcatc ggccgcgctc
     6061 ccgattccgg aagtgcttga cattggggaa ttcagcgaga gcctgaccta ttgcatctcc
     6121 cgccgtgcac agggtgtcac gttgcaagac ctgcctgaaa ccgaactgcc cgctgttctg
     6181 cagccggtcg cggaggccat ggatgcgatc gctgcggccg atcttagcca gacgagcggg
     6241 ttcggcccat tcggaccgca aggaatcggt caatacacta catggcgtga tttcatatgc
     6301 gcgattgctg atccccatgt gtatcactgg caaactgtga tggacgacac cgtcagtgcg
     6361 tccgtcgcgc aggctctcga tgagctgatg ctttgggccg aggactgccc cgaagtccgg
     6421 cacctcgtgc acgcggattt cggctccaac aatgtcctga cggacaatgg ccgcataaca
     6481 gcggtcattg actggagcga ggcgatgttc ggggattccc aatacgaggt cgccaacatc
     6541 ttcttctgga ggccgtggtt ggcttgtatg gagcagcaga cgcgctactt cgagcggagg
     6601 catccggagc ttgcaggatc gccgcggctc cgggcgtata tgctccgcat tggtcttgac
     6661 caactctatc agagcttggt tgacggcaat ttcgatgatg cagcttgggc gcagggtcga
     6721 tgcgacgcaa tcgtccgatc cggagccggg actgtcgggc gtacacaaat cgcccgcaga
     6781 agcgcggccg tctggaccga tggctgtgta gaagtactcg ccgatagtgg aaaccgacgc
     6841 cccagcactc gtccgagggc aaaggaatag cacgtactac gagatttcga ttccaccgcc
     6901 gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc
     6961 cagcgcgggg atctcatgct ggagttcttc gcccacccca acttgtttat tgcagcttat
     7021 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     7081 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg tataccgtcg
     7141 acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
     7201 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
     7261 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     7321 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     7381 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     7441 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     7501 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     7561 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     7621 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     7681 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     7741 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     7801 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     7861 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     7921 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     7981 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     8041 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     8101 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     8161 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     8221 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     8281 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     8341 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     8401 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     8461 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     8521 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     8581 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     8641 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     8701 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     8761 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     8821 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     8881 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     8941 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     9001 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     9061 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     9121 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     9181 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     9241 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     9301 tttccccgaa aagtgccacc tgacgtcgac ggatcgggag atctcccgat cccctatggt
     9361 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
     9421 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
     9481 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
     9541 tacgggccag atatacgcgt tgacattgat tattgactag gcttttgcaa aaagctttgc
     9601 aaagatggat aaagttttaa acagagagga atctttgcag ctaatggacc ttctaggtct
     9661 tgaaaggagt gggaatt
//
