LOCUS       sequence-376908-       14023 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To express a hybrid Trk receptor tagged with EGFP. This Hybrid TRK 
            receptor was designed to bind NGF, BDNF and NT-3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14023)
  AUTHORS   O'Connell L, Hongo JA, Presta LG, Tsoulfas P
  TITLE     TrkA amino acids controlling specificity for nerve growth factor.
  JOURNAL   J Biol Chem. 2000 Mar 17;275(11):7870-7. doi: 
            10.1074/jbc.275.11.7870.
  PUBMED    10713102
REFERENCE   2  (bases 1 to 14023)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.275.11"; journalName: "J Biol Chem"; date: "2000-03-17-
            17"; volume: "275"; issue: "11"; pages: "7870-7"
COMMENT     Sequence Label: pLZRS-panTRK-CR1-EGFP
FEATURES             Location/Qualifiers
     source          1..14023
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     22..41
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(260..364)
                     /gene="bla"
                     /label=AmpR promoter
     LTR             901..1370
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    1435..1792
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1857..2273
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     2203..2225
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     2241..2257
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    2283..2657
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     2595..2611
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     primer_bind     2696..2712
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2696..2712
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             join(5180..5182,5183..5185,5186..5698)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIE"
     primer_bind     complement(5225..5246)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5486..5505)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5833..5854
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             6081..6550
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(6680..6697)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6851..7439)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6931..6950)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    7562..7696
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7586..7605)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7640..7659
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(7926..8525)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8009..8029)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     8506..8525
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(8608..9107)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(8656..8675)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     9049..9072
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     rep_origin      11139..12928
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     CDS             complement(join(13422..14023,1..190,191..259))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
       61 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
      121 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
      181 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
      241 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
      301 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
      361 cgcgcacatt tccccgaaaa gtgccaccag ctttgctctt aggagtttcc taatacatcc
      421 caaactcaaa tatataaagc atttgacttg ttctatgccc tagggggcgg ggggaagcta
      481 agccagcttt ttttaacatt taaaatgtta attccatttt aaatgcacag atgtttttat
      541 ttcataaggg tttcaatgtg catgaatgct gcaatattcc tgttaccaaa gctagtataa
      601 ataaaaatag ataaacgtgg aaattactta gagtttctgt cattaacgtt tccttcctca
      661 gttgacaaca taaatgcgct gctgagaagc cagtttgcat ctgtcaggat caatttccca
      721 ttatgccagt catattaatt actagtcaat tagttgattt ttatttttga catatacatg
      781 tgaaagaccc cacctgtagg tttggcaagc tagcttaagt aacgccattt tgcaaggcat
      841 ggaaaaatac ataactgaga atagaaaagt tcagatcaag gtcaggaaca gatggaacag
      901 ctgaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc tcagggccaa
      961 gaacagatgg aacagctgaa tatgggccaa acaggatatc tgtggtaagc agttcctgcc
     1021 ccggctcagg gccaagaaca gatggtcccc agatgcggtc cagccctcag cagtttctag
     1081 agaaccatca gatgtttcca gggtgcccca aggacctgaa atgaccctgt gccttatttg
     1141 aactaaccaa tcagttcgct tctcgcttct gttcgcgcgc ttctgctccc cgagctcaat
     1201 aaaagagccc acaacccctc actcggcgcg ccagtcctcc gattgactga gtcgcccggg
     1261 tacccgtgta tccaataaac cctcttgcag ttgcatccga cttgtggtct cgctgttcct
     1321 tgggagggtc tcctctgagt gattgactac ccgtcagcgg gggtctttca tttgggggct
     1381 cgtccgggat cgggagaccc ctgcccaggg accaccgacc caccaccggg aggtaagctg
     1441 gccagcaact tatctgtgtc tgtccgattg tctagtgtct atgactgatt ttatgcgcct
     1501 gcgtcggtac tagttagcta actagctctg tatctggcgg acccgtggtg gaactgacga
     1561 gttcggaaca cccggccgca accctgggag acgtcccagg gacttcgggg gccgtttttg
     1621 tggcccgacc tgagtccaaa aatcccgatc gttttggact ctttggtgca ccccccttag
     1681 aggagggata tgtggttctg gtaggagacg agaacctaaa acagttcccg cctccgtctg
     1741 aatttttgct ttcggtttgg gaccgaagcc gcgccgcgcg tcttgtctgc tgcagcatcg
     1801 ttctgtgttg tctctgtctg actgtgtttc tgtatttgtc tgaaaatatg ggcccgggcc
     1861 agactgttac cactccctta agtttgacct taggtcactg gaaagatgtc gagcggatcg
     1921 ctcacaacca gtcggtagat gtcaagaaga gacgttgggt taccttctgc tctgcagaat
     1981 ggccaacctt taacgtcgga tggccgcgag acggcacctt taaccgagac ctcatcaccc
     2041 aggttaagat caaggtcttt tcacctggcc cgcatggaca cccagaccag gtcccctaca
     2101 tcgtgacctg ggaagccttg gcttttgacc cccctccctg ggtcaagccc tttgtacacc
     2161 ctaagcctcc gcctcctctt cctccatccg ccccgtctct cccccttgaa cctcctcgtt
     2221 cgaccccgcc tcgatcctcc ctttatccag ccctcactcc ttctctaggc gcccccatat
     2281 ggccatatga gatcttatat ggggcacccc cgccccttgt aaacttccct gaccctgaca
     2341 tgacaagagt tactaacagc ccctctctcc aagctcactt acaggctctc tacttagtcc
     2401 agcacgaagt ctggagacct ctggcggcag cctaccaaga acaactggac cgaccggtgg
     2461 tacctcaccc ttaccgagtc ggcgacacag tgtgggtccg ccgacaccag actaagaacc
     2521 tagaacctcg ctggaaagga ccttacacag tcctgctgac cacccccacc gccctcaaag
     2581 tagacggcat cgcagcttgg atacacgccg cccacgtgaa ggctgccgac cccgggggtg
     2641 gaccatcctc tagactgccg gatcccagtg tggtggtacg ggaattcgcg gccgctctag
     2701 aactagtgga tcccccgggc tgcaggaatt cgattcgacc atggatgtct ctctttgccc
     2761 agccaagtgt agtttctggc ggattttctt gctgggaagc gtctggctgg actatgtggg
     2821 ctccgtgctg gcttgccctg caaattgtgt ctgcagcaag actgagatca attgccggcg
     2881 gccggacgat gggaacctct tccccctcct ggaagggcag gattcaggga acagcaatgg
     2941 gaacgccaat atcaacatca cggacatctc aaggaatatc acttccatac acatagagaa
     3001 ctggcgcagt cttcacacgc tcaacgccgt ggacatggag ctctacaccg gacttcaaaa
     3061 gctgaccatc aagaactcag gacttcggag cattcagccc agagcctttg ccaagaaccc
     3121 ccatttgcgt tatataaacc tgtcaagtaa ccggctcacc acactctcgt ggcagctctt
     3181 ccagacgctg agtcttcggg aattgcagtt ggagcagaac tttttcaact gcagctgtga
     3241 catccgctgg atgcagctct ggcaggagca gggggaggcc aagctcaaca gccagaacct
     3301 ctactgcatc aatgctgatg gctcccagct tcctctcttc cgcatgaaca tcagtcagtg
     3361 tgaccttcct gagatcagcg tgagccacgt caacctgacc gtacgagagg gtgacaatgc
     3421 tgttatcact tgcaatggct ctggatcacc ccttcctgat gtggactgga tagtcactgg
     3481 gctgcagtcc atcaacactc accagaccaa tctgaactgg accaatgttc atgccatcaa
     3541 cttgacgctg gtgaatgtga cgagtgagga caatggcttc accctgacgt gcattgcaga
     3601 gaacgtggtg ggcatgagca atgccagtgt tgccctcact gtctactatc ccccacgtgt
     3661 ggtgagcctg gaggaaccgg tggagatgca tcactggtgc atcccgttct cggtggacgg
     3721 caacccccca ccaacgctgc actggctgca caatggaagc gtgctgaacg agactagttt
     3781 catcttcacg gaattcctcg agcctgcagc caatgagacc gtgcggcacg gctgcctgct
     3841 cttcaacaag cctactcacg tcaacaatgg caactatacc ctgatagcca agaacccact
     3901 tggtaccgcc aatcagacca tcaatggaca cttcatggat gagcccttcc catttgacga
     3961 agaggatccc atacctgaca ctaacagtac ttccggagac ccggtggaga agaaggacga
     4021 aacacctttt ggggtctcgg tggctgtggg cctggccgtc tttgcctgcc tcttcctttc
     4081 tacgctgctc cttgtgctca acaaatgtgg acggagaaac aagtttggga tcaaccgccc
     4141 ggctgtgctg gctccagagg atgggctggc catgtccctg catttcatga cattgggtgg
     4201 cagctccctg tcccccaccg agggcaaagg ctctgggctc caaggccaca tcatcgagaa
     4261 cccacaatac ttcagtgatg cctgtgttca ccacatcaag cgccgggaca tcgtgctcaa
     4321 gtgggagctg ggggagggcg cctttgggaa ggtcttcctt gctgagtgcc acaacctcct
     4381 gcctgagcag gacaagatgc tggtggctgt caaggcactg aaggaggcgt ccgagagtgc
     4441 tcggcaggac ttccaacgtg aggctgagct gctcaccatg ctgcagcacc agcacatcgt
     4501 gcgcttcttc ggcgtctgca ccgagggccg ccccctgctc atggtctttg agtatatgcg
     4561 gcacggggac ctcaaccgct tcctccgatc ccatggacct gatgccaagc tgctggctgg
     4621 tggggaggat gtggctccag gccccctggg tctggggcag ctgctggccg tggctagcca
     4681 ggtcgctgcg gggatggtgt acctggcggg tctgcatttt gtgcaccggg acctggccac
     4741 acgcaactgt ctagtgggcc agggactggt ggtcaagatt ggtgattttg gcatgagcag
     4801 ggatatctac agcaccgact attaccgtgt gggaggccgc accatgctgc ccattcgctg
     4861 gatgccgccc gagagcatcc tgtaccgtaa gttcaccacc gagagcgacg tgtggagctt
     4921 cggcgtggtg ctctgggaga tcttcaccta cggcaagcag ccctggtacc agctctccaa
     4981 cacggaggca atcgactgca tcacgcaggg acgtgagttg gagcggccac gtgcctgccc
     5041 accagaggtc tacgccatca tgcggggctg ctggcagcgg gagccccagc aacgccacag
     5101 catcaaggat gtgcacgccc ggctgcaagc cctggcccag gcacctcctg tctacctgga
     5161 tgtcctgggc gaattcacca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc
     5221 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg
     5281 cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct
     5341 gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg
     5401 ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt
     5461 ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa
     5521 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga
     5581 cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg tctatatcat
     5641 ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga
     5701 cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt
     5761 gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga
     5821 gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca tcaagcttat
     5881 cgataccgtc gaggggccgc cagcacagtg gtcgacgata aaataaaaga ttttatttag
     5941 tctccagaaa aaggggggaa tgaaagaccc cacctgtagg tttggcaagc tagcttaagt
     6001 aacgccattt tgcaaggcat ggaaaaatac ataactgaga atagagaagt tcagatcaag
     6061 gtcaggaaca gatggaacag ctgaatatgg gccaaacagg atatctgtgg taagcagttc
     6121 ctgccccggc tcagggccaa gaacagatgg aacagctgaa tatgggccaa acaggatatc
     6181 tgtggtaagc agttcctgcc ccggctcagg gccaagaaca gatggtcccc agatgcggtc
     6241 cagccctcag cagtttctag agaaccatca gatgtttcca gggtgcccca aggacctgaa
     6301 atgaccctgt gccttatttg aactaaccaa tcagttcgct tctcgcttct gttcgcgcgc
     6361 ttctgctccc cgagctcaat aaaagagccc acaacccctc actcggggcg ccagtcctcc
     6421 gattgactga gtcgcccggg tacccgtgta tccaataaac cctcttgcag ttgcatccga
     6481 cttgtggtct cgctgttcct tgggagggtc tcctctgagt gattgactac ccgtcagcgg
     6541 gggtctttca catgcagcat gtatcaaaat taatttggtt ttttttctta agtatttaca
     6601 ttaaatggcc atagttgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     6661 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     6721 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     6781 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     6841 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     6901 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     6961 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     7021 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     7081 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     7141 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     7201 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     7261 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     7321 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     7381 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     7441 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     7501 ggattttggt catgagatta tcaaaaagga tcttcaccta gatccttttg cggccgaatt
     7561 agatccagac atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg
     7621 aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag
     7681 ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga
     7741 ggtgtgggag gttttttaaa gcaagtaaaa cctctacaaa tgtggtatgg ctgattatga
     7801 tcctctagag tcggtgggcc tcgggggcgg gtgcggggtc ggcggggccg ccccgggtgg
     7861 cttcggtcgg agccatgggg tcgtgcgctc ctttcggtcg ggcgctgcgg gtcgtggggc
     7921 gggcgtcagg caccgggctt gcgggtcatg caccaggtgc gcggtccttc gggcacctcg
     7981 acgtcggcgg tgacggtgaa gccgagccgc tcgtagaagg ggaggttgcg gggcgcggag
     8041 gtctccagga aggcgggcac cccggcgcgc tcggccgcct ccactccggg gagcacgacg
     8101 gcgctgccca gacccttgcc ctggtggtcg ggcgagacgc cgacggtggc caggaaccac
     8161 gcgggctcct tgggccggtg cggcgccagg aggccttcca tctgttgctg cgcggccagc
     8221 cgggaaccgc tcaactcggc catgcgcggg ccgatctcgg cgaacaccgc ccccgcttcg
     8281 acgctctccg gcgtggtcca gaccgccacc gcggcgccgt cgtccgcgac ccacaccttg
     8341 ccgatgtcga gcccgacgcg cgtgaggaag agttcttgca gctcggtgac ccgctcgatg
     8401 tggcggtccg ggtcgacggt gtggcgcgtg gcggggtagt cggcgaacgc ggcggcgagg
     8461 gtgcgtacgg cccgggggac gtcgtcgcgg gtggcgaggc gcaccgtggg cttgtactcg
     8521 gtcatggaag gtcgtctcct tgtgaggggt caggggcgtg ggtcagggga tggtggcggc
     8581 accggtcgtg gcggccgacc tgcaggtcga aaggcccgga gatgaggaag aggagaacag
     8641 cgcggcagac gtgcgctttt gaagcgtgca gaatgccggg cctccggagg accttcgggc
     8701 gcccgccccg cccctgagcc cgcccctgag cccgcccccg gacccacccc ttcccagcct
     8761 ctgagcccag aaagcgaagg agcaaagctg ctattggccg ctgccccaaa ggcctacccg
     8821 cttccattgc tcagcggtgc tgtccatctg cacgagacta gtgagacgtg ctacttccat
     8881 ttgtcacgtc ctgcacgacg cgagctgcgg ggcggggggg aacttcctga ctaggggagg
     8941 agtagaaggt ggcgcgaagg ggccaccaaa gaacggagcc ggttggcgcc taccggtgga
     9001 tgtggaatgt gtgcgaggcc agaggccact tgtgtagcgc caagtgccca gcggggctgc
     9061 taaagcgcat gctccagact gccttgggaa aagcgcctcc cctacccggt agaattaatt
     9121 ctcatgtttg acagcttatc atcgataagc tgatcctcac aggccgcacc cagcttttct
     9181 tccgttgccc cagtagcatc tctgtctggt gaccttgaag aggaagagga ggggtcccga
     9241 gaatccccat ccctaccgtc cagcaaaaag ggggacgagg aatttgaggc ctggcttgag
     9301 gctcaggacg caaatcttga ggatgttcag cgggagtttt ccgggctgcg agtaattggt
     9361 gatgaggacg aggatggttc ggaggatggg gaattttcag acctggatct gtctgacagc
     9421 gaccatgaag gggatgaggg tgggggggct gttggagggg gcaggagtct gcactccctg
     9481 tattcactga gcgtcgtcta ataaagatgt ctattgatct cttttagtgt gaatcatgtc
     9541 tgacgagggg ccaggtacag gacctggaaa tggcctagga gagaagggag acacatctgg
     9601 accagaaggc tccggcggca gtggacctca aagaagaggg ggtgataacc atggacgagg
     9661 acggggaaga ggacgaggac gaggaggcgg aagaccagga gccccgggcg gctcaggatc
     9721 agggccaaga catagagatg gtgtccggag accccaaaaa cgtccaagtt gcattggctg
     9781 caaagggacc cacggtggaa caggagcagg agcaggagcg ggaggggcag gagcaggagg
     9841 ggcaggagca ggaggagggg caggagcagg aggggcagga gcaggaggtg gaggccgggg
     9901 tcgaggaggc agtggaggcc ggggtcgagg aggtagtgga ggccggggtc gaggaggtag
     9961 tggaggccgc cggggtagag gacgtgaaag agccaggggg ggaagtcgtg aaagagccag
    10021 ggggagaggt cgtggacgtg gagaaaagag gcccaggagt cccagtagtc agtcatcatc
    10081 atccgggtct ccaccgcgca ggccccctcc aggtagaagg ccatttttcc accctgtagg
    10141 ggaagccgat tattttgaat accaccaaga aggtggccca gatggtgagc ctgacgtgcc
    10201 cccgggagcg atagagcagg gccccgcaga tgacccagga gaaggcccaa gcactggacc
    10261 ccggggtcag ggtgatggag gcaggcgcaa aaaaggaggg tggtttggaa agcatcgtgg
    10321 tcaaggaggt tccaacccga aatttgagaa cattgcagaa ggtttaagag ctctcctggc
    10381 taggagtcac gtagaaagga ctaccgacga aggaacttgg gtcgccggtg tgttcgtata
    10441 tggaggtagt aagacctccc tttacaacct aaggcgagga actgcccttg ctattccaca
    10501 atgtcgtctt acaccattga gtcgtctccc ctttggaatg gcccctggac ccggcccaca
    10561 acctggcccg ctaagggagt ccattgtctg ttatttcatg gtctttttac aaactcatat
    10621 atttgctgag gttttgaagg atgcgattaa ggaccttgtt atgacaaagc ccgctcctac
    10681 ctgcaatatc agggtgactg tgtgcagctt tgacgatgga gtagatttgc ctccctggtt
    10741 tccacctatg gtggaagggg ctgccgcgga gggtgatgac ggagatgacg gagatgaagg
    10801 aggtgatgga gatgagggtg aggaagggca ggagtgatgt aacttgttag gagacgccct
    10861 caatcgtatt aaaagccgtg tattcccccg cactaaagaa taaatcccca gtagacatca
    10921 tgcgtgctgt tggtgtattt ctggccatct gtcttgtcac cattttcgtc ctcccaacat
    10981 ggggcaattg ggcataccca tgttgtcacg tcactcagct ccgcgctcaa caccttctcg
    11041 cgttggaaaa cattagcgac atttacctgg tgagcaatca gacatgcgac ggctttagcc
    11101 tggcctcctt aaattcacct aagaatggga gcaaccagca ggaaaaggac aagcagcgaa
    11161 aattcacgcc cccttgggag gtggcggcat atgcaaagga tagcactccc actctactac
    11221 tgggtatcat atgctgactg tatatgcatg aggatagcat atgctacccg gatacagatt
    11281 aggatagcat atactaccca gatatagatt aggatagcat atgctaccca gatatagatt
    11341 aggatagcct atgctaccca gatataaatt aggatagcat atactaccca gatatagatt
    11401 aggatagcat atgctaccca gatatagatt aggatagcct atgctaccca gatatagatt
    11461 aggatagcat atgctaccca gatatagatt aggatagcat atgctatcca gatatttggg
    11521 tagtatatgc tacccagata taaattagga tagcatatac taccctaatc tctattagga
    11581 tagcatatgc tacccggata cagattagga tagcatatac tacccagata tagattagga
    11641 tagcatatgc tacccagata tagattagga tagcctatgc tacccagata taaattagga
    11701 tagcatatac tacccagata tagattagga tagcatatgc tacccagata tagattagga
    11761 tagcctatgc tacccagata tagattagga tagcatatgc tatccagata tttgggtagt
    11821 atatgctacc catggcaaca ttagcccacc gtgctctcag cgacctcgtg aatatgagga
    11881 ccaacaaccc tgtgcttggc gctcaggcgc aagtgtgtgt aatttgtcct ccagatcgca
    11941 gcaatcgcgc ccctatcttg gcccgcccac ctacttatgc aggtattccc cggggtgcca
    12001 ttagtggttt tgtgggcaag tggtttgacc gcagtggtta gcggggttac aatcagccaa
    12061 gttattacac ccttatttta cagtccaaaa ccgcagggcg gcgtgtgggg gctgacgcgt
    12121 gcccccactc cacaatttca aaaaaaagag tggccacttg tctttgttta tgggccccat
    12181 tggcgtggag ccccgtttaa ttttcggggg tgttagagac aaccagtgga gtccgctgct
    12241 gtcggcgtcc actctctttc cccttgttac aaatagagtg taacaacatg gttcacctgt
    12301 cttggtccct gcctgggaca catcttaata accccagtat catattgcac taggattatg
    12361 tgttgcccat agccataaat tcgtgtgaga tggacatcca gtctttacgg cttgtcccca
    12421 ccccatggat ttctattgtt aaagatattc agaatgtttc attcctacac tagtatttat
    12481 tgcccaaggg gtttgtgagg gttatattgg tgtcatagca caatgccacc actgaacccc
    12541 ccgtccaaat tttattctgg gggcgtcacc tgaaaccttg ttttcgagca cctcacatac
    12601 accttactgt tcacaactca gcagttattc tattagctaa acgaaggaga atgaagaagc
    12661 aggcgaagat tcaggagagt tcactgcccg ctccttgatc ttcagccact gcccttgtga
    12721 ctaaaatggt tcactaccct cgtggaatcc tgaccccatg taaataaaac cgtgacagct
    12781 catggggtgg gagatatcgc tgttccttag gaccctttta ctaaccctaa ttcgatagca
    12841 tatgcttccc gttgggtaac atatgctatt gaattagggt tagtctggat agtatatact
    12901 actacccggg aagcatatgc tacccgttta gggttaacaa gggggcctta taaacactat
    12961 tgctaatgcc ctcttgaggg tccgcttatc ggtagctaca caggcccctc tgattgacgt
    13021 tggtgtagcc tcccgtagtc ttcctgggcc cctgggaggt acatgtcccc cagcattggt
    13081 gtaagagctt cagccaagag ttacacataa aggcaatgtt gtgttgcagt ccacagactg
    13141 caaagtctgc tccaggatga aagccactca gtgttggcaa atgtgcacat ccatttataa
    13201 ggatgtcaac tacagtcaga gaaccccttt gtgtttggtc cccccccgtg tcacatgtgg
    13261 aacagggccc agttggcaag ttgtaccaac caactgaagg gattacatgc actgccccgc
    13321 gaagaagggg cagagatgtc gtagtcaggt ttagttcgtc cggggcgggg atctggccgc
    13381 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
    13441 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
    13501 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
    13561 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
    13621 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
    13681 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
    13741 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
    13801 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
    13861 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
    13921 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
    13981 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gct
//
