LOCUS       sequence-376911-        8067 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8067)
  AUTHORS   Banaszynski LA, Chen LC, Maynard-Smith LA, Ooi AG, Wandless TJ
  TITLE     A rapid, reversible, and tunable method to regulate protein function
            in living cells using synthetic small molecules.
  JOURNAL   Cell. 2006 Sep 8;126(5):995-1004.
  PUBMED    16959577
REFERENCE   2  (bases 1 to 8067)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell."; 
            date: "2006-09-8"; volume: "126(5)"; pages: "995-1004"
COMMENT     Sequence Label: pBMN FKBP(DD)-YFP
FEATURES             Location/Qualifiers
     source          1..8067
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             867..1336
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    1401..1758
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1823..2239
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     2169..2191
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     2207..2223
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    2249..2623
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     2561..2577
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     regulatory      2632..2641
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2638..2640,2641..2961)
                     /codon_start=1
                     /product="destabilization domain that can be stabilized by 
                     Shield1 in the ProteoTuner(TM) system"
                     /label=DD
                     /note="L106P mutant of FKBP12"
                     /translation="MGVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKVDSSRDRNK
                     PFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVEL
                     LKPE"
     CDS             join(2968..2970,2971..3681)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(3010..3031)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3271..3290)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3618..3639
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             3688..3714
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    join(3771..4344,4345..4347,4348..4356)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3937..3954)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4164..4183
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4357..5040
                     /codon_start=1
                     /product="far-red fluorescent variant of Heteractis crispa 
                     chromoprotein"
                     /label=HcRed1
                     /note="mammalian codon-optimized"
                     /translation="MVSGLLKESMRIKMYMEGTVNGHYFKCEGEGDGNPFAGTQSMRIH
                     VTEGAPLPFAFDILAPCCEYGSRTFVHHTAEIPDFFKQSFPEGFTWERTTTYEDGGILT
                     AHQDTSLEGNCLIYKVKVHGTNFPADGPVMKNKSGGWEPSTEVVYPENGVLCGRNVMAL
                     KVGDRHLICHHYTSYRSKKAVRALTMPGFHFTDIRLQMLRKKKDEYFELYEASVARYSD
                     LPEKAN"
     CDS             5053..5739
                     /codon_start=1
                     /product="far-red fluorescent variant of Heteractis crispa 
                     chromoprotein"
                     /label=HcRed1
                     /note="mammalian codon-optimized"
                     /translation="MVSGLLKESMRIKMYMEGTVNGHYFKCEGEGDGNPFAGTQSMRIH
                     VTEGAPLPFAFDILAPCCEYGSRTFVHHTAEIPDFFKQSFPEGFTWERTTTYEDGGILT
                     AHQDTSLEGNCLIYKVKVHGTNFPADGPVMKNKSGGWEPSTEVVYPENGVLCGRNVMAL
                     KVGDRHLICHHYTSYRSKKAVRALTMPGFHFTDIRLQMLRKKKDEYFELYEASVARYSD
                     LPEKAN"
     LTR             5910..6379
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(6509..6526)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6680..7268)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6760..6779)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             join(7392..7460,7461..8067,1..185)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7610..7629)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
       61 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
      121 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
      181 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttgcgg ccggccgcaa
      241 aaggatctag gtgaagatcc tttttgataa tctcatgagc ggatacatat ttgaatgtat
      301 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc caccagcttt
      361 gctcttagga gtttcctaat acatcccaaa ctcaaatata taaagcattt gacttgttct
      421 atgccctagg gggcgggggg aagctaagcc agcttttttt aacatttaaa atgttaattc
      481 cattttaaat gcacagatgt ttttatttca taagggtttc aatgtgcatg aatgctgcaa
      541 tattcctgtt accaaagcta gtataaataa aaatagataa acgtggaaat tacttagagt
      601 ttctgtcatt aacgtttcct tcctcagttg acaacataaa tgcgctgctg agaagccagt
      661 ttgcatctgt caggatcaat ttcccattat gccagtcata ttaattacta gtcaattagt
      721 tgatttttat ttttgacata tacatgtgaa agaccccacc tgtaggtttg gcaagctagc
      781 ttaagtaacg ccattttgca aggcatggaa aaatacataa ctgagaatag aaaagttcag
      841 atcaaggtca ggaacagatg gaacagctga atatgggcca aacaggatat ctgtggtaag
      901 cagttcctgc cccggctcag ggccaagaac agatggaaca gctgaatatg ggccaaacag
      961 gatatctgtg gtaagcagtt cctgccccgg ctcagggcca agaacagatg gtccccagat
     1021 gcggtccagc cctcagcagt ttctagagaa ccatcagatg tttccagggt gccccaagga
     1081 cctgaaatga ccctgtgcct tatttgaact aaccaatcag ttcgcttctc gcttctgttc
     1141 gcgcgcttct gctccccgag ctcaataaaa gagcccacaa cccctcactc ggcgcgccag
     1201 tcctccgatt gactgagtcg cccgggtacc cgtgtatcca ataaaccctc ttgcagttgc
     1261 atccgacttg tggtctcgct gttccttggg agggtctcct ctgagtgatt gactacccgt
     1321 cagcgggggt ctttcatttg ggggctcgtc cgggatcggg agacccctgc ccagggacca
     1381 ccgacccacc accgggaggt aagctggcca gcaacttatc tgtgtctgtc cgattgtcta
     1441 gtgtctatga ctgattttat gcgcctgcgt cggtactagt tagctaacta gctctgtatc
     1501 tggcggaccc gtggtggaac tgacgagttc ggaacacccg gccgcaaccc tgggagacgt
     1561 cccagggact tcgggggccg tttttgtggc ccgacctgag tccaaaaatc ccgatcgttt
     1621 tggactcttt ggtgcacccc ccttagagga gggatatgtg gttctggtag gagacgagaa
     1681 cctaaaacag ttcccgcctc cgtctgaatt tttgctttcg gtttgggacc gaagccgcgc
     1741 cgcgcgtctt gtctgctgca gcatcgttct gtgttgtctc tgtctgactg tgtttctgta
     1801 tttgtctgaa aatatgggcc cgggccagac tgttaccact cccttaagtt tgaccttagg
     1861 tcactggaaa gatgtcgagc ggatcgctca caaccagtcg gtagatgtca agaagagacg
     1921 ttgggttacc ttctgctctg cagaatggcc aacctttaac gtcggatggc cgcgagacgg
     1981 cacctttaac cgagacctca tcacccaggt taagatcaag gtcttttcac ctggcccgca
     2041 tggacaccca gaccaggtcc cctacatcgt gacctgggaa gccttggctt ttgacccccc
     2101 tccctgggtc aagccctttg tacaccctaa gcctccgcct cctcttcctc catccgcccc
     2161 gtctctcccc cttgaacctc ctcgttcgac cccgcctcga tcctcccttt atccagccct
     2221 cactccttct ctaggcgccc ccatatggcc atatgagatc ttatatgggg cacccccgcc
     2281 ccttgtaaac ttccctgacc ctgacatgac aagagttact aacagcccct ctctccaagc
     2341 tcacttacag gctctctact tagtccagca cgaagtctgg agacctctgg cggcagccta
     2401 ccaagaacaa ctggaccgac cggtggtacc tcacccttac cgagtcggcg acacagtgtg
     2461 ggtccgccga caccagacta agaacctaga acctcgctgg aaaggacctt acacagtcct
     2521 gctgaccacc cccaccgccc tcaaagtaga cggcatcgca gcttggatac acgccgccca
     2581 cgtgaaggct gccgaccccg ggggtggacc atcctctaga ctgccggatc cgccaccatg
     2641 ggagtgcagg tggaaaccat ctccccagga gacgggcgca ccttccccaa gcgcggccag
     2701 acctgtgtgg tgcactacac cgggatgctt gaagatggaa agaaagtcga ttcctcccgg
     2761 gacagaaaca agccctttaa gtttatgcta ggcaagcagg aggtgatccg aggctgggaa
     2821 gaaggggttg cccagatgag tgtgggtcag agagccaaac tgactatatc tccagattat
     2881 gcctatggtg ccactgggca cccaggcatc atcccaccac atgccactct cgtcttcgat
     2941 gtggagcttc taaaaccgga agaattcgtg agcaagggcg aggagctgtt caccggggtg
     3001 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc
     3061 gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc
     3121 aagctgcccg tgccctggcc caccctcgtg accaccttcg gctacggcct gcagtgcttc
     3181 gcccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc
     3241 tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag
     3301 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag
     3361 gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat
     3421 atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc
     3481 gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc
     3541 cccgtgctgc tgcccgacaa ccactacctg agctaccagt ccgccctgag caaagacccc
     3601 aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc
     3661 ggcatggacg agctgtacaa ggtcgactat ccgtacgacg taccagacta cgcactcgac
     3721 taactcgagg gccggcgcgc cgcggccgct acgtaaattc csshcccccc cccctctccc
     3781 yccccccccc taacgttact ggccgaagcc gcttggaata aggccggtgt gcgtttgtct
     3841 atatgttatt ttccaccata ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc
     3901 ctgtcttctt gacgagcatt cctaggggtc tttcccctct cgccaaagga atgcaaggtc
     3961 tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc ttgaagacaa acaacgtctg
     4021 tagcgaccct ttgcaggcag cggaaccccc cacctggcga caggtgcctc tgcggccaaa
     4081 agccacgtgt ataagataca cctgcaaagg cggcacaacc ccagtgccac gttgtgagtt
     4141 ggatagttgt ggaaagagtc aaatggctct cctcaagcgt attcaacaag gggctgaagg
     4201 atgcccagaa ggtaccccat tgtatgggat ctgatctggg gcctcggtgc acatgcttta
     4261 catgtgttta gtcgaggtta aaaaaacgtc taggcccccc gaaccacggg gacgtggttt
     4321 tcctttgaaa aacacgatga taatatggcc acaaccatgg tgagcggcct gctgaaggag
     4381 agtatgcgca tcaagatgta catggagggc accgtgaacg gccactactt caagtgcgag
     4441 ggcgagggcg acggcaaccc cttcgccggc acccagagca tgagaatcca cgtgaccgag
     4501 ggcgcccccc tgcccttcgc cttcgacatc ctggccccct gctgcgagta cggcagcagg
     4561 accttcgtgc accacaccgc cgagatcccc gacttcttca agcagagctt ccccgagggc
     4621 ttcacctggg agagaaccac cacctacgag gacggcggca tcctgaccgc ccaccaggac
     4681 accagcctgg agggcaactg cctgatctac aaggtgaagg tgcacggcac caacttcccc
     4741 gccgacggcc ccgtgatgaa gaacaagagc ggcggctggg agcccagcac cgaggtggtg
     4801 taccccgaga acggcgtgct gtgcggccgg aacgtgatgg ccctgaaggt gggcgaccgg
     4861 cacctgatct gccaccacta caccagctac cggagcaaga aggccgtgcg cgccctgacc
     4921 atgcccggct tccacttcac cgacatccgg ctccagatgc tgcggaagaa gaaggacgag
     4981 tacttcgagc tgtacgaggc cagcgtggcc cggtacagcg acctgcccga gaaggccaac
     5041 agatctcccg ggatggtgag cggcctgctg aaggagagta tgcgcatcaa gatgtacatg
     5101 gagggcaccg tgaacggcca ctacttcaag tgcgagggcg agggcgacgg caaccccttc
     5161 gccggcaccc agagcatgag aatccacgtg accgagggcg cccccctgcc cttcgccttc
     5221 gacatcctgg ccccctgctg cgagtacggc agcaggacct tcgtgcacca caccgccgag
     5281 atccccgact tcttcaagca gagcttcccc gagggcttca cctgggagag aaccaccacc
     5341 tacgaggacg gcggcatcct gaccgcccac caggacacca gcctggaggg caactgcctg
     5401 atctacaagg tgaaggtgca cggcaccaac ttccccgccg acggccccgt gatgaagaac
     5461 aagagcggcg gctgggagcc cagcaccgag gtggtgtacc ccgagaacgg cgtgctgtgc
     5521 ggccggaacg tgatggccct gaaggtgggc gaccggcacc tgatctgcca ccactacacc
     5581 agctaccgga gcaagaaggc cgtgcgcgcc ctgaccatgc ccggcttcca cttcaccgac
     5641 atccggctcc agatgctgcg gaagaagaag gacgagtact tcgagctgta cgaggccagc
     5701 gtggcccggt acagcgacct gcccgagaag gccaactgag tcgacgataa aataaaagat
     5761 tttatttagt ctccagaaaa aggggggaat gaaagacccc acctgtaggt ttggcaagct
     5821 agcttaagta acgccatttt gcaaggcatg gaaaaataca taactgagaa tagagaagtt
     5881 cagatcaagg tcaggaacag atggaacagc tgaatatggg ccaaacagga tatctgtggt
     5941 aagcagttcc tgccccggct cagggccaag aacagatgga acagctgaat atgggccaaa
     6001 caggatatct gtggtaagca gttcctgccc cggctcaggg ccaagaacag atggtcccca
     6061 gatgcggtcc agccctcagc agtttctaga gaaccatcag atgtttccag ggtgccccaa
     6121 ggacctgaaa tgaccctgtg ccttatttga actaaccaat cagttcgctt ctcgcttctg
     6181 ttcgcgcgct tctgctcccc gagctcaata aaagagccca caacccctca ctcggggcgc
     6241 cagtcctccg attgactgag tcgcccgggt acccgtgtat ccaataaacc ctcttgcagt
     6301 tgcatccgac ttgtggtctc gctgttcctt gggagggtct cctctgagtg attgactacc
     6361 cgtcagcggg ggtctttcac atgcagcatg tatcaaaatt aatttggttt tttttcttaa
     6421 gtatttacat taaatggcca tagttgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     6481 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     6541 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     6601 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     6661 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     6721 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     6781 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     6841 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     6901 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     6961 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     7021 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     7081 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     7141 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     7201 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     7261 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     7321 cacgttaagg gattttggtc atgagacaat aaccctgata aatgcttcaa taatattgaa
     7381 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     7441 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     7501 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     7561 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     7621 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     7681 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     7741 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     7801 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     7861 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     7921 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     7981 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     8041 cacttctgcg ctcggccctt ccggctg
//
