LOCUS       sequence-376914-       11044 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of FIP200 mutant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11044)
  AUTHORS   Shi X, Chang C, Yokom AL, Jensen LE, Hurley JH
  TITLE     The autophagy adaptor NDP52 and the FIP200 coiled-coil 
            allosterically activate ULK1 complex membrane recruitment.
  JOURNAL   Elife. 2020 Aug 10;9. pii: 59099. doi: 10.7554/eLife.59099.
  PUBMED    32773036
REFERENCE   2  (bases 1 to 11044)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2020-08-10"; volume: "9. pii: 59099. doi"; pages: " 
            10.7554/eLife.59099"
COMMENT     Sequence Label: pCAG-GST-hFIP200(Delta641-779, 1363-1370Mut)-MBP
FEATURES             Location/Qualifiers
     source          1..11044
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        5..384
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        386..663
                     /label=chicken beta-actin promoter
     intron          664..1680
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1604..1626
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1688..1707
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      1754..1763
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1760..2416
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MASPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNK
                     KFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIR
                     YGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVV
                     LYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     2374..2396
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     CDS             2444..2464
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             6848..7948
                     /codon_start=1
                     /gene="malE (mutated)"
                     /product="maltose binding protein from E. coli"
                     /label=MBP
                     /note="This version of the gene does not encode a signal 
                     sequence, so MBP will remain in the cytosol."
                     /translation="MKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLE
                     EKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKL
                     IAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIA
                     ADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGE
                     TAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFL
                     ENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAF
                     WYAVRTAVINAASGRQTVDEALKDAQT"
     primer_bind     7922..7945
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     primer_bind     complement(8066..8085)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    8131..8186
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(8185..8204)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(8547..8563)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8547..8563)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8560..8582)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8571..8587
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8595..8601,8602..8619,8620..8625))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8640..8661
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        8720..8915
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8766..8901
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    8921..9055
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8958..8977)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9012..9031
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9123..9140)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9294..9882)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9374..9393)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10053..10844,10845..10913))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10676..10695
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10914..11018)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ggtcgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat
       61 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
      121 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
      181 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
      241 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
      301 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
      361 gtattagtca tcgctattac catggtcgag gtgagcccca cgttctgctt cactctcccc
      421 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca
      481 gcgatggggg cggggggggg gggggggcgc gcgccaggcg gggcggggcg gggcgagggg
      541 cggggcgggg cgaggcggag aggtgcggcg gcagccaatc agagcggcgc gctccgaaag
      601 tttcctttta tggcgaggcg gcggcggcgg cggccctata aaaagcgaag cgcgcggcgg
      661 gcgggagtcg ctgcgcgctg ccttcgcccc gtgccccgct ccgccgccgc ctcgcgccgc
      721 ccgccccggc tctgactgac cgcgttactc ccacaggtga gcgggcggga cggcccttct
      781 cctccgggct gtaattagcg cttggtttaa tgacggcttg tttcttttct gtggctgcgt
      841 gaaagccttg aggggctccg ggagggccct ttgtgcgggg ggagcggctc ggggggtgcg
      901 tgcgtgtgtg tgtgcgtggg gagcgccgcg tgcggctccg cgctgcccgg cggctgtgag
      961 cgctgcgggc gcggcgcggg gctttgtgcg ctccgcagtg tgcgcgaggg gagcgcggcc
     1021 gggggcggtg ccccgcggtg cggggggggc tgcgagggga acaaaggctg cgtgcggggt
     1081 gtgtgcgtgg gggggtgagc agggggtgtg ggcgcgtcgg tcgggctgca accccccctg
     1141 cacccccctc cccgagttgc tgagcacggc ccggcttcgg gtgcggggct ccgtacgggg
     1201 cgtggcgcgg ggctcgccgt gccgggcggg gggtggcggc aggtgggggt gccgggcggg
     1261 gcggggccgc ctcgggccgg ggagggctcg ggggaggggc gcggcggccc ccggagcgcc
     1321 ggcggctgtc gaggcgcggc gagccgcagc cattgccttt tatggtaatc gtgcgagagg
     1381 gcgcagggac ttcctttgtc ccaaatctgt gcggagccga aatctgggag gcgccgccgc
     1441 accccctcta gcgggcgcgg ggcgaagcgg tgcggcgccg gcaggaagga aatgggcggg
     1501 gagggccttc gtgcgtcgcc gcgccgccgt ccccttctcc ctctccagcc tcggggctgt
     1561 ccgcgggggg acggctgcct tcggggggga cggggcaggg cggggttcgg cttctggcgt
     1621 gtgaccggcg gctctagagc ctctgctaac catgttcatg ccttcttctt tttcctacag
     1681 ctcctgggca acgtgctggt tattgtgctg tctcatcatt ttggcaaaga attcctcgat
     1741 cgacggtatc gatgccacca tggcatcccc tatactaggt tattggaaaa ttaagggcct
     1801 tgtgcaaccc actcgacttc ttttggaata tcttgaagaa aaatatgaag agcatttgta
     1861 tgagcgcgat gaaggtgata aatggcgaaa caaaaagttt gaattgggtt tggagtttcc
     1921 caatcttcct tattatattg atggtgatgt taaattaaca cagtctatgg ccatcatacg
     1981 ttatatagct gacaagcaca acatgttggg tggttgtcca aaagagcgtg cagagatttc
     2041 aatgcttgaa ggagcggttt tggatattag atacggtgtt tcgagaattg catatagtaa
     2101 agactttgaa actctcaaag ttgattttct tagcaagcta cctgaaatgc tgaaaatgtt
     2161 cgaagatcgt ttatgtcata aaacatattt aaatggtgat catgtaaccc atcctgactt
     2221 catgttgtat gacgctcttg atgttgtttt atacatggac ccaatgtgcc tggatgcgtt
     2281 cccaaaatta gtttgtttta aaaaacgtat tgaagctatc ccacaaattg ataagtactt
     2341 gaaatccagc aagtatatag catggccttt gcagggctgg caagccacgt ttggtggtgg
     2401 cgaccatcct ccaaaatcgg atgattacga tatcccaacg accgaaaacc tgtattttca
     2461 gggtggtacc atgaagctct atgtgtttct cgtgaatacc ggcactaccc tgacctttga
     2521 taccgaactg accgtccaga ccgtggctga cctgaagcat gctatccagt ctaaatacaa
     2581 gatcgcaatt cagcaccagg tgctggtggt caacggcgga gagtgcatgg ccgctgatcg
     2641 cagggtctgt acctattcag ccggcaccga cactaaccca atcttcctct ttaataagga
     2701 gatgattctg tgcgatagac cccctgctat ccccaagacc acttttagca ctgagaacga
     2761 catggaaatc aaagtggagg aatccctgat gatgccagcc gtgttccaca ccgtcgcttc
     2821 tcgcactcag ctggcactcg agatgtacga agtggccaag aaactgtgct ctttctgtga
     2881 gggtctcgtc cacgatgaac atctgcagca ccagggctgg gcagccatta tggctaacct
     2941 ggaggactgt tcaaatagtt atcagaaact gctcttcaag tttgagtcta tctactcaaa
     3001 ctatctgcag agcatcgaag acattaaact gaagctcaca cacctgggaa ccgcagtgag
     3061 cgtcatggcc aagatccctc tgctcgagtg cctgactagg cattcctaca gagaatgtct
     3121 gggtaggctc gatagtctgc cagaacacga ggacagcgag aaagccgaaa tgaagcgctc
     3181 tacagagctg gtgctctcac ctgacatgcc aaggacaacc aacgaaagtc tgctcaccag
     3241 cttcccaaag tccgtggagc atgtctcccc cgacactgcc gatgctgaat ctggaaagga
     3301 gattagagaa agttgccaga gcaccgtgca ccagcaggac gagactacaa tcgatactaa
     3361 ggacggcgat ctgcccttct ttaacgtcag tctgctcgat tggatcaacg tccaggacag
     3421 acctaatgat gtggagtccc tggtccggaa gtgctttgat agcatgtccc gtctggaccc
     3481 acgtatcatt cgtcctttca tcgcagagtg tcgtcagacc attgccaagc tggacaacca
     3541 gaatatgaaa gctattaagg gtctggaaga tcggctctac gccctggacc agatgatcgc
     3601 tagctgtgga cgcctggtga acgagcagaa ggaactcgct cagggtttcc tggcaaacca
     3661 gaaaagagcc gagaatctca aggatgcatc agtgctgcct gacctgtgcc tcagtcatgc
     3721 caatcagctg atgattatgc tccagaacca ccgcaagctg ctcgacatca aacagaagtg
     3781 taccactgct aaacaggagc tggcaaacaa tctccatgtg aggctgaagt ggtgctgttt
     3841 cgtcatgctg cacgcagacc aggatggcga gaagctccag gccctgctca ggctggtgat
     3901 tgagctgctc gaaagagtga agatcgtcga ggctctgtcc accgtcccac agatgtactg
     3961 cctggcagtg gtcgaggtgg tcagacgtaa aatgtttatc aagcactaca gagaatgggc
     4021 aggtgccctc gtgaaagatg gcaagcgtct gtatgaagcc gagaaatcta agcgtgagtc
     4081 attcggcaaa ctgtttcgga agagcttcct ccgtaaccgg ctgttccgcg gactcgacag
     4141 ttggccaccc agcttttgca cccagaaacc taggaagttc gactgtgagc tgccagatat
     4201 ctccctcaag gacctgcagt ttctccagtc cttctgccca tctgaggtgc agccctttct
     4261 gagggtccct ctgctctgtg acttcgaacc tctgcaccag catgtgctgg ccctccacaa
     4321 cctcgtcaag gctgcacaga gtctggatga gatgagccag acaatcaccg acctgctcag
     4381 cgaacagaag acaaatgtgt gcggcaagga ggactttgga gatcatacct ctctgaacgt
     4441 ccagctcgaa cgctgtaggg tggtcgctca ggattctcat ttctcaatcc agactattaa
     4501 ggaggacctg tgccacttcc gcacatttgt gcagaaggaa cagtgcgact tcagtaacag
     4561 cctgaaatgt acagccgtgg agatcaggaa catcatcgaa aaggtcaagt gttctctgga
     4621 gatcaccctc aaagaaaagc accagaaaga gctgctctca ctgaagaacg aatacgaggg
     4681 aaaactggac ggtctcatta aggaaaccga ggaaaacgag aataaaatca agaaactgaa
     4741 gggagagctg gtgtgcctcg aggaagtcct gcagaacaag gataatgaat tcgccctggt
     4801 gaaacatgaa aaggaggctg tcatctgcct gcagaatgag aaagaccaga agctgctcga
     4861 aatggagaac attatgcact cacagaattg tgagatcaaa gaactgaagc agagtagaga
     4921 gatcgtgctg gaagatctca agaaactgca tgtcgagaat gacgaaaagc tgcagctgct
     4981 ccgtgccgaa ctccagagcc tggagcagtc ccacctcaag gaactggagg atactctgca
     5041 ggtgcgccat atccaggaat tcgagaaggt gatgacagac cacagagtca gcctcgagga
     5101 actgaagaaa gagaaccagc agatcattaa tcagatccag gagtcccacg ctgaaatcat
     5161 tcaggaaaaa gagaagcagc tgcaggagct gaaactcaag gtgtctgacc tcagcgatac
     5221 ccgctgcaaa ctggaagtcg agctggccct caaggaagct gagactgacg agatcaaaat
     5281 tctgctcgag gaatctcggg cccagcagaa agaaaccctg aagtcactgc tcgaacagga
     5341 gaccgaaaat ctccgtactg agatcagcaa actgaaccag aagattcagg ataacaatga
     5401 gaattaccaa gtgggactcg ctgaactgag aactctcatg acaatcgaga aggaccagtg
     5461 tatctctgaa ctgatttcac gtcatgagga agagtcaaac attctgaaag cagagctcaa
     5521 taaagtgact agtctgcaca accaggcctt cgagatcgag aagaacctga aggagcagat
     5581 cattgaactg cagtccaagc tcgattccga actgtctgcc ctcgagaggc agaaagacga
     5641 aaagatcaca cagcaggaag agaagtatga ggctatcatt cagaacctcg aaaaagatag
     5701 gcagaagctg gtgtcctctc aggagcagga cagagaacag ctgattcaga aactcaattg
     5761 cgagaaggac gaagcaatcc agacagccct caaagagttc aagctggaga gagaagtggt
     5821 cgagaaagaa ctgctcgaga aagtgaagca cctggaaaac cagatcgcta agtcacccgc
     5881 aattgatagt acccgtggtg actcaagtag cctggtggcc gagctccagg aaaagctgca
     5941 ggaagagaag gctaagtttc tggagcagct cgaagagcag gaaaaaagaa agaacgaaga
     6001 gatgcagaat gtgcgtacaa gcctgatcgc cgagcagcag accaacttca ataccgtcct
     6061 gactcgtgag aaaatgcgga aggaaaacat cattaatgac ctcagcgata aactgaagtc
     6121 caccatgcag cagcaggagg gctcatctgg cggttccagc ggactgtctg aagacagagc
     6181 tcgtctgctc gaagagaaga aaaagctgga agaggaagtc agtaagctga ggtcctcttc
     6241 attcgtgcca agcccctacg tcgctactgc acccgagctg tatggagcct gcgctcctga
     6301 gctgcctggt gaatcagacc ggagtgccgt ggagactgct gatgaaggcc gcgtcgactc
     6361 tgccatggag acaagcatga tgtccgtgca ggaaaacatt catatgctgt cagaggaaaa
     6421 gcagagaatc atgctgctcg agcgtaccct gcagctcaaa gaggaagaga acaagcgcct
     6481 caatcagagg ctgatgtcac agagtatgag tagcgtgtcc tctcgccact ccgagaagat
     6541 cgccattagg gatttccaag tgggagacct ggtcctcatc attctggacg aacgccatga
     6601 taactacgtg ctgtttactg tctctcctac actgtatttc ctccacagcg agtccctgcc
     6661 cgctctggac ctcaagcctg gtgaaggcgc atctggcgcc tcaaggagac cctgggtgct
     6721 gggaaaagtc atggagaagg aatactgcca ggctaaaaag gcacagaacc gttttaaggt
     6781 gcctctgggc accaaattct atcgggtgaa ggccgtctcc tggaataaaa aggtccccgg
     6841 ggctagcatg aaaatcgaag aaggtaaact ggtaatctgg attaacggcg ataaaggcta
     6901 taacggtctc gctgaagtcg gtaagaaatt cgagaaagat accggaatta aagtcaccgt
     6961 tgagcatccg gataaactgg aagagaaatt cccacaggtt gcggcaactg gcgatggccc
     7021 tgacattatc ttctgggcac acgaccgctt tggtggctac gctcaatctg gcctgttggc
     7081 tgaaatcacc ccggacaaag cgttccagga caagctgtat ccgtttacct gggatgccgt
     7141 acgttacaac ggcaagctga ttgcttaccc gatcgctgtt gaagcgttat cgctgattta
     7201 taacaaagat ctgctgccga acccgccaaa aacctgggaa gagatcccgg cgctggataa
     7261 agaactgaaa gcgaaaggta agagcgcgct gatgttcaac ctgcaagaac cgtacttcac
     7321 ctggccgctg attgctgctg acgggggtta tgcgttcaag tatgaaaacg gcaagtacga
     7381 cattaaagac gtgggcgtgg ataacgctgg cgcgaaagcg ggtctgacct tcctggttga
     7441 cctgattaaa aacaaacaca tgaatgcaga caccgattac tccatcgcag aagctgcctt
     7501 taataaaggc gaaacagcga tgaccatcaa cggcccgtgg gcatggtcca acatcgacac
     7561 cagcaaagtg aattatggtg taacggtact gccgaccttc aagggtcaac catccaaacc
     7621 gttcgttggc gtgctgagcg caggtattaa cgccgccagt ccgaacaaag agctggcaaa
     7681 agagttcctc gaaaactatc tgctgactga tgaaggtctg gaagcggtta ataaagacaa
     7741 accgctgggt gccgtagcgc tgaagtctta cgaggaagag ttggcgaaag atccacgtat
     7801 tgccgccact atggaaaacg cccagaaagg tgaaatcatg ccgaacatcc cgcagatgtc
     7861 cgctttctgg tatgccgtgc gtactgcggt gatcaacgcc gccagcggtc gtcagactgt
     7921 cgatgaagcc ctgaaagacg cgcagactaa tcccgggtga tatcttaatt aattaactcg
     7981 aggaattcac tcctcaggtg caggctgcct atcagaaggt ggtggctggt gtggccaatg
     8041 ccctggctca caaataccac tgagatcttt ttccctctgc caaaaattat ggggacatca
     8101 tgaagcccct tgagcatctg acttctggct aataaaggaa atttattttc attgcaatag
     8161 tgtgttggaa ttttttgtgt ctctcactcg gaaggacata tgggagggca aatcatttaa
     8221 aacatcagaa tgagtatttg gtttagagtt tggcaacata tgcccatatg ctggctgcca
     8281 tgaacaaagg ttggctataa agaggtcatc agtatatgaa acagccccct gctgtccatt
     8341 ccttattcca tagaaaagcc ttgacttgag gttagatttt ttttatattt tgttttgtgt
     8401 tatttttttc tttaacatcc ctaaaatttt ccttacatgt tttactagcc agatttttcc
     8461 tcctctcctg actactccca gtcatagctg tccctcttct cttatggaga tccctcgacc
     8521 tgcagcccaa gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
     8581 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
     8641 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
     8701 ctgtcgtgcc agcggatccg catctcaatt agtcagcaac catagtcccg cccctaactc
     8761 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
     8821 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt
     8881 gaggaggctt ttttggaggc ctaggctttt gcaaaaagct aacttgttta ttgcagctta
     8941 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     9001 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatccgctg
     9061 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
     9121 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     9181 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     9241 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     9301 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     9361 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     9421 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     9481 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     9541 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     9601 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     9661 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     9721 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     9781 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     9841 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     9901 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     9961 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
    10021 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
    10081 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
    10141 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
    10201 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
    10261 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
    10321 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
    10381 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
    10441 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
    10501 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
    10561 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
    10621 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
    10681 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
    10741 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
    10801 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
    10861 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
    10921 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
    10981 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
    11041 cctg
//
