LOCUS       sequence-376941-       11271 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For knocking pCAG-EGFPnls into AAVS1 locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11271)
  AUTHORS   Xu P, He H, Gao Q, Zhou Y, Wu Z, Zhang X, Sun L, Hu G, Guan Q, You 
            Z, Zhang X, Zheng W, Xiong M, Chen Y
  TITLE     Human midbrain dopaminergic neuronal differentiation markers predict
            cell therapy outcomes in a Parkinson's disease model.
  JOURNAL   J Clin Invest. 2022 Jul 15;132(14). pii: 156768. doi: 
            10.1172/JCI156768.
  PUBMED    35700056
REFERENCE   2  (bases 1 to 11271)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Clin 
            Invest."; date: "2022-07-15"; volume: "132(14). pii: 156768. doi"; 
            pages: " 10.1172/JCI156768"
COMMENT     Sequence Label: AAVS1-NeoR-CAG-EGFPnls-WPRE
FEATURES             Location/Qualifiers
     source          1..11271
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    37..840
                     /label=HA-L
                     /note="left homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     misc_feature    847..872
                     /label=SA
                     /note="splice acceptor site"
     CDS             896..949
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             953..1756
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1788..1805)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    1794..2018
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     enhancer        2086..2465
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          2745..3761
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     3685..3707
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     3769..3788
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      3825..3834
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3831..3833,3834..3836,3837..4547)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3831..3833,3834..3836,3837..4547)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3876..3897)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4137..4156)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4484..4505
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             4548..4595
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     misc_feature    4632..5220
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4685..4705)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5103..5114)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    5252..5728
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(5451..5470)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     primer_bind     complement(5838..5857)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    5903..5958
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(5957..5976)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(6319..6335)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6343..6359
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6367..6373,6374..6391,6392..6397))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6412..6433
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    6507..7343
                     /label=HA-R
                     /note="right homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     primer_bind     complement(7377..7396)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7378..7396)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7403..7420)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7403..7419)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7412..7434)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             7557..7859
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="QFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKV
                     SRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     7738..7757
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             complement(join(9256..10047,10048..10116))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9879..9898
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10240..10828
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10729..10748
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10982..10999
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11116..11137
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11152..11157,11158..11175,11176..11182)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11190..11206
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11214..11230
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        11251..11269
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ctagctagtc ctgcaggttt aaacgaattc gccctttgct ttctctgacc agcattctct
       61 cccctgggcc tgtgccgctt tctgtctgca gcttgtggcc tgggtcacct ctacggctgg
      121 cccagatcct tccctgccgc ctccttcagg ttccgtcttc ctccactccc tcttcccctt
      181 gctctctgct gtgttgctgc ccaaggatgc tctttccgga gcacttcctt ctcggcgctg
      241 caccacgtga tgtcctctga gcggatcctc cccgtgtctg ggtcctctcc gggcatctct
      301 cctccctcac ccaaccccat gccgtcttca ctcgctgggt tcccttttcc ttctccttct
      361 ggggcctgtg ccatctctcg tttcttagga tggccttctc cgacggatgt ctcccttgcg
      421 tcccgcctcc ccttcttgta ggcctgcatc atcaccgttt ttctggacaa ccccaaagta
      481 ccccgtctcc ctggctttag ccacctctcc atcctcttgc tttctttgcc tggacacccc
      541 gttctcctgt ggattcgggt cacctctcac tcctttcatt tgggcagctc ccctaccccc
      601 cttacctctc tagtctgtgc tagctcttcc agccccctgt catggcatct tccaggggtc
      661 cgagagctca gctagtcttc ttcctccaac ccgggcccct atgtccactt caggacagca
      721 tgtttgctgc ctccagggat cctgtgtccc cgagctggga ccaccttata ttcccagggc
      781 cggttaatgt ggctctggtt ctgggtactt ttatctgtcc cctccacccc acagtggggc
      841 aagcttctga cctcttctct tcctcccaca gggcctcgag agatctggca gcggagaggg
      901 cagaggaagt cttctaacat gcggtgacgt ggaggagaat cccggcccta ggatgggatc
      961 ggccattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt
     1021 cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc
     1081 agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact
     1141 gcaggacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt
     1201 gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca
     1261 ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat
     1321 gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg
     1381 catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga
     1441 agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gcatgcccga
     1501 cggcgatgat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa
     1561 tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga
     1621 catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt
     1681 cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct
     1741 tgacgagttc ttctgagggg atcaattctc tagagctcgc tgatcagcct cgactgtgcc
     1801 ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg
     1861 tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag
     1921 gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga
     1981 caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag
     2041 ctggggctcg actagagctt gcggaaccct taatggtacc gtctcgacat tgattattga
     2101 ctagttatta atagtaatca attacggggt cattagttca tagcccatat atggagttcc
     2161 gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat
     2221 tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc
     2281 aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc
     2341 caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt
     2401 acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta
     2461 ccatggtcga ggtgagcccc acgttctgct tcactctccc catctccccc ccctccccac
     2521 ccccaatttt gtatttattt attttttaat tattttgtgc agcgatgggg gcgggggggg
     2581 ggggggggcg cgcgccrggs gggggggggg gggggggggg ggggggsggg gsgrggsgga
     2641 gaggtgcggc ggcagccaat cagagcggcg cgctccgaaa gtttcctttt atggcgaggc
     2701 ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg ggcgggagtc gctgcgcgct
     2761 gccttcgccc cgtgccccgc tccgccgccg cctcgcgccg cccgccccgg ctctgactga
     2821 ccgcgttact cccacaggtg agcgggcggg acggcccttc tcctccgggc tgtaattagc
     2881 gcttggttta atgacggctt gtttcttttc tgtggctgcg tgaaagcctt gaggggctcc
     2941 gggagggccc tttgtgcggg gggagcggct cggggggtgc gtgcgtgtgt gtgtgcgtgg
     3001 ggagcgccgc gtgcggctcc gcgctgcccg gcggctgtga gcgctgcggg cgcggcgcgg
     3061 ggctttgtgc gctccgcagt gtgcgcgagg ggagcgcggc cgggggcggt gccccgcggt
     3121 gcgggggggg ctgcgagggg aacaaaggct gcgtgcgggg tgtgtgcgtg ggggggtgag
     3181 cagggggtgt gggcgcgtcg gtcgggctgc aaccccccct gcacccccct ccccgagttg
     3241 ctgagcacgg cccggcttcg ggtgcggggc tccgtacggg gcgtggcgcg gggctcgccg
     3301 tgccgggcgg ggggtggcgg caggtggggg tgccgggcgg ggcggggccg cctcgggccg
     3361 gggrgggctc gggggrgggg cgcggcggcc cccggagcgc cggcggctgt cgaggcgcgg
     3421 cgagccgcag ccattgcctt ttatggtaat cgtgcgagag ggcgcaggga cttcctttgt
     3481 cccaaatctg tgcggagccg aaatctggga ggcgccgccg caccccctct agcgggcgcg
     3541 gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg ggagggcctt cgtgcgtcgc
     3601 cgcgccgccg tccccttctc cctctccagc ctcggggctg tccgcggggg gacggctgcc
     3661 ttcggggggg acggggcagg gcggggttcg gcttctggcg tgtgaccggc ggctctagag
     3721 cctctgctaa ccatgttcat gccttcttct ttttcctaca gctcctgggc aacgtgctgg
     3781 ttattgtgct gtctcatcat tttggcaaag aattcgtcga cgccgccacc atggtgagca
     3841 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa
     3901 acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga
     3961 ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca
     4021 ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact
     4081 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg
     4141 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca
     4201 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt
     4261 acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg
     4321 tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc
     4381 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca
     4441 cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt
     4501 tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagaag cgacctgccg
     4561 ccacaaagaa ggctggacag gctaagaaga agaaataatg aacgcgtgaa ttcgatatca
     4621 agcttatcga taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta
     4681 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
     4741 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt
     4801 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg
     4861 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt
     4921 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
     4981 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc
     5041 cttggctgct cgcctatgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc
     5101 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc
     5161 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc
     5221 atcgataccg agcgctgctc gagagatcta cgggtggcat ccctgtgacc cctccccagt
     5281 gcctctcctg gccctggaag ttgccactcc agtgcccacc agccttgtcc taataaaatt
     5341 aagttgcatc attttgtctg actaggtgtc cttctataat attatggggt ggaggggggt
     5401 ggtatggagc aaggggcaag ttgggaagac aacctgtagg gcctgcgggg tctattggga
     5461 accaagctgg agtgcagtgg cacaatcttg gctcactgca atctccgcct cctgggttca
     5521 agcgattctc ctgcctcagc ctcccgagtt gttgggattc caggcatgca tgaccaggct
     5581 cagctaattt ttgttttttt ggtagagacg gggtttcacc atattggcca ggctggtctc
     5641 caactcctaa tctcaggtga tctacccacc ttggcctccc aaattgctgg gattacaggc
     5701 gtgaaccact gctcccttcc ctgtccttct gattttgtag gtaaccaggc gcgtgaattc
     5761 actcctcagg tgcaggctgc ctatcagaag gtggtggctg gtgtggccaa tgccctggct
     5821 cacaaatacc actgagatct ttttccctct gccaaaaatt atggggacat catgaagccc
     5881 cttgagcatc tgacttctgg ctaataaagg aaatttattt tcattgcaat agtgtgttgg
     5941 aattttttgt gtctctcact cggaaggaca tatgggaggg caaatcattt aaaacatcag
     6001 aatgagtatt tggtttagag tttggcaaca tatgcccata tgctggctgc catgaacaaa
     6061 ggttggctat aaagaggtca tcagtatatg aaacagcccc ctgctgtcca ttccttattc
     6121 catagaaaag ccttgacttg aggttagatt ttttttatat tttgttttgt gttatttttt
     6181 tctttaacat ccctaaaatt ttccttacat gttttactag ccagattttt cctcctctcc
     6241 tgactactcc cagtcatagc tgtccctctt ctcttatgga gatccctcga cctgcagccc
     6301 aagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     6361 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     6421 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     6481 ccagcggatc gacagtacta agctttacta gggacaggat tggtgacaga aaagccccat
     6541 ccttaggcct cctccttcct agtctcctga tattgggtct aacccccacc tcctgttagg
     6601 cagattcctt atctggtgac acacccccat ttcctggagc catctctctc cttgccagaa
     6661 cctctaaggt ttgcttacga tggagccaga gaggatcctg ggagggagag cttggcaggg
     6721 ggtgggaggg aaggggggga tgcgtgacct gcccggttct cagtggccac cctgcgctac
     6781 cctctcccag aacctgagct gctctgacgc ggctgtctgg tgcgtttcac tgatcctggt
     6841 gctgcagctt ccttacactt cccaagagga gaagcagttt ggaaaaacaa aatcagaata
     6901 agttggtcct gagttctaac tttggctctt cacctttcta gtccccaatt tatattgttc
     6961 ctccgtgcgt cagttttacc tgtgagataa ggccagtagc cagccccgtc ctggcagggc
     7021 tgtggtgagg aggggggtgt ccgtgtggaa aactcccttt gtgagaatgg tgcgtcctag
     7081 gtgttcacca ggtcgtggcc gcctctactc cctttctctt tctccatcct tctttcctta
     7141 aagagtcccc agtgctatct gggacatatt cctccgccca gagcagggtc ccgcttccct
     7201 aaggccctgc tctgggcttc tgggtttgag tccttggcaa gcccaggaga ggcgctcagg
     7261 cttccctgtc ccccttcctc gtccaccatc tcatgcccct ggctctcctg ccccttccct
     7321 acaggggttc ctggctctgc tctaagggcg aattcgcggc cgctaaattc aattcgccct
     7381 atagtgagtc gtattacaat tcactggccg tcgttttaca acgtcgtgac tgggaaaacc
     7441 ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata
     7501 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctatac gtacggcagt
     7561 ttaaggttta cacctataaa agagagagcc gttatcgtct gtttgtggat gtacagagtg
     7621 atattattga cacgccgggg cgacggatgg tgatccccct ggccagtgca cgtctgctgt
     7681 cagataaagt ctcccgtgaa ctttacccgg tggtgcatat cggggatgaa agctggcgca
     7741 tgatgaccac cgatatggcc agtgtgccgg tctccgttat cggggaagaa gtggctgatc
     7801 tcagccaccg cgaaaatgac atcaaaaacg ccattaacct gatgttctgg ggaatataaa
     7861 tgtcaggcat gagattatca aaaaggatct tcacctagat ccttttcacg tagaaagcca
     7921 gtccgcagaa acggtgctga ccccggatga atgtcagcta ctgggctatc tggacaaggg
     7981 aaaacgcaag cgcaaagaga aagcaggtag cttgcagtgg gcttacatgg cgatagctag
     8041 actgggcggt tttatggaca gcaagcgaac cggaattgcc agctggggcg ccctctggta
     8101 aggttgggaa gccctgcaaa gtaaactgga tggctttctt gccgccaagg atctgatggc
     8161 gcaggggatc aagctctgat caagagacag gatgaggatc gtttcgcatg attgaacaag
     8221 atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg
     8281 cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc
     8341 cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcaa gacgaggcag
     8401 cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca
     8461 ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat
     8521 ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata
     8581 cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac
     8641 gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc
     8701 tcgcgccagc cgaactgttc gccaggctca aggcgagcat gcccgacggc gaggatctcg
     8761 tcgtgaccca tgcatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt
     8821 tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg
     8881 gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt
     8941 tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc
     9001 ttctgaatta ttaacgctta caatttcctg atgcggtatt ttctccttac gcatctgtgc
     9061 ggtatttcac accgcatcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     9121 tttatttttc taaatacatt caaatatgta tccgctcatg agattatcaa aaaggatctt
     9181 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     9241 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     9301 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     9361 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     9421 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     9481 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     9541 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     9601 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     9661 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     9721 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     9781 cgtaagatgc ttttctgtga ctggtgaata ctcaaccaag tcattctgag aatagtgtat
     9841 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     9901 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     9961 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
    10021 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
    10081 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
    10141 aagcatttat cagggttatt gtctcatgac caaaatccct taacgtgagt tttcgttcca
    10201 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
    10261 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
    10321 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
    10381 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
    10441 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
    10501 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
    10561 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
    10621 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
    10681 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
    10741 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
    10801 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
    10861 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
    10921 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
    10981 cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc
    11041 gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag
    11101 tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt
    11161 tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa
    11221 cagctatgac catgattacg ccaagctcag aattaaccct cactaaaggg a
//
