LOCUS       sequence-376960-        9873 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CMV-driven expression of an untagged mutant NPRL2 (core subunit of 
            GATOR1) — native sequence for stable expression in mammalian cells. 
            All lysine residues were mutated to arginines..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9873)
  AUTHORS   Valenstein ML, Rogala KB, Lalgudi PV, Brignole EJ, Gu X, Saxton RA, 
            Chantranupong L, Kolibius J, Quast JP, Sabatini DM
  TITLE     Structure of the nutrient-sensing hub GATOR2.
  JOURNAL   Nature. 2022 Jul;607(7919):610-616. doi: 10.1038/s41586-022-04939-z.
            Epub 2022 Jul 13.
  PUBMED    35831510
REFERENCE   2  (bases 1 to 9873)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-022-04939-z"; journalName: "Nature"; date: 
            "2022-07"; volume: "607"; issue: "7919"; pages: "610-616"
COMMENT     Sequence Label: K-to-R mutant NPRL2(1-380) in pLJM60
FEATURES             Location/Qualifiers
     source          1..9873
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     61..78
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    195..216
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(231..236,237..254,255..261)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    269..285
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     274..296
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     293..309
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     293..309
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     328..348
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        330..348
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        376..602
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             603..783
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    830..955
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1452..1685
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3208..3252
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        3559..3762
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3713..3733
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     3759..3783
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        3993..4011
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     3993..4010
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     4024..4043
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             4094..4120
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    5342..5459
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        5508..6018
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     5937..5955
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             6040..6639
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6040..6059)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6536..6556
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             6767..7000
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7072..7193
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7109..7128)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7163..7182
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7233..7368
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7388..7407)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7389..7407)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7417..7434)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7417..7433)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7426..7448)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7575..8030
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7662..7681)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7872..7893
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8056..8160
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8161..8229,8230..9021)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8379..8398)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9192..9780
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9681..9700
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
       61 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg
      121 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt
      181 gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc tttacacttt
      241 atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac
      301 agctatgacc atgattacgc caagcgcgca attaaccctc actaaaggga acaaaagctg
      361 gagctgcaag cttaatgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga
      421 tgagttagca acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag
      481 taaggtggta cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac
      541 gaaccactga attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacataa
      601 acgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
      661 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
      721 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
      781 gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg
      841 caggactcgg cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac
      901 gccaaaaatt ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat
      961 taagcggggg agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa
     1021 aaaatataaa ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa
     1081 tcctggcctg ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc
     1141 ccttcagaca ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg
     1201 tgtgcatcaa aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga
     1261 gcaaaacaaa agtaagacca ccgcacagca agcggccggc cgctgatctt cagacctgga
     1321 ggaggagata tgagggacaa ttggagaagt gaattatata aatataaagt agtaaaaatt
     1381 gaaccattag gagtagcacc caccaaggca aagagaagag tggtgcagag agaaaaaaga
     1441 gcagtgggaa taggagcttt gttccttggg ttcttgggag cagcaggaag cactatgggc
     1501 gcagcgtcaa tgacgctgac ggtacaggcc agacaattat tgtctggtat agtgcagcag
     1561 cagaacaatt tgctgagggc tattgaggcg caacagcatc tgttgcaact cacagtctgg
     1621 ggcatcaagc agctccaggc aagaatcctg gctgtggaaa gatacctaaa ggatcaacag
     1681 ctcctgggga tttggggttg ctctggaaaa ctcatttgca ccactgctgt gccctgatga
     1741 atcccctaat gattttggta aaaatcatta agttaaggtg gatacacatc ttgtcatatg
     1801 atcaaatggt ttcgcgaaaa atcaataatc agacaacaag atgtgcgaac tcgatatttt
     1861 acacgactct ctttaccaat tctgccccga attacactta aaacgactca acagcttaac
     1921 gttggcttgc cacgccttac ttgactgtaa aactctcact cttaccgaac ttggccgtaa
     1981 cctgccaacc aaagcgagaa caaaacataa catcaaacga atcgaccgat tgttaggtaa
     2041 tcgtcacctc cacaaagagc gactcgctgt ataccgttgg catgctagct ttatctgttc
     2101 gggcaatacg atgcccattg tacttgttga ctggtctgat attcgtgagc aaaaacgact
     2161 tatggtattg cgagcttcag tcgcactaca cggtcgttct gttactcttt atgagaaagc
     2221 gttcccgctt tcagagcaat gttcaaagaa agctcatgac caatttctag ccgaccttgc
     2281 gagcattcta ccgagtaaca ccacaccgct cattgtcagt gatgctggct ttaaagtgcc
     2341 atggtataaa tccgttgaga agctgggttg gtactggtta agtcgagtaa gaggaaaagt
     2401 acaatatgca gacctaggag cggaaaactg gaaacctatc agcaacttac atgatatgtc
     2461 atctagtcac tcaaagactt taggctataa gaggctgact aaaagcaatc caatctcatg
     2521 ccaaattcta ttgtataaat ctcgctctaa aggccgaaaa aatcagcgct cgacacggac
     2581 tcattgtcac cacccgtcac ctaaaatcta ctcagcgtcg gcaaaggagc catgggttct
     2641 agcaactaac ttacctgttg aaattcgaac acccaaacaa cttgttaata tctattcgaa
     2701 gcgaatgcag attgaagaaa ccttccgaga cttgaaaagt cctgcctacg gactaggcct
     2761 acgccatagc cgaacgagca gctcagagcg ttttgatatc atgctgctaa tcgccctgat
     2821 gcttcaacta acatgttggc ttgcgggcgt tcatgctcag aaacaaggtt gggacaagca
     2881 cttccaggct aacacagtca gaaatcgaaa cgtactctca acagttcgct taggcatgga
     2941 agttttgcgg cattctggct acacaataac aagggaagac ttactcgtgg ctgcaaccct
     3001 actagctcaa aatttattca cacatggtta cgctttgggg aaattatgag gggatctctc
     3061 agtgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac
     3121 acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta
     3181 attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa
     3241 tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc
     3301 ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg
     3361 aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg
     3421 ggaccccata tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg
     3481 cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta
     3541 gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg
     3601 tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg
     3661 caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg
     3721 ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctcgtttagt gaaccgtcag
     3781 atcgcctgga gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc
     3841 agcctccgcg gccgggaacg gtgcattgga acgcggattc cccgtgccaa gagtgacgta
     3901 agtaccgcct atagagtcta taggcccacc cccttggctt cgttagaacg cggctacaat
     3961 taatacataa ccttatgtat catacacata cgatttaggt gacactatag aataacatcc
     4021 actttgcctt tctctccaca ggtgtccact cccaggtcca actgcacctc ggttctatcg
     4081 attcaccatg ggatacccat acgatgttcc agattacgct ggtggtggtg gtggtgcgtc
     4141 gacgatgggc agcggctgcc gcatcgaatg catattcttc agcgagttcc accccacgct
     4201 gggacccagg atcacctatc aggtccctga agacttcatc tcccgagagc tgtttgacac
     4261 agtccaagtg tacatcatca ccaggccaga gctgcagaac aggcttatca ctgtcacagc
     4321 tatggaaagg aggctgatcg gctgtcctgt gtgcatcgaa cacaggaggt acagccgcaa
     4381 tgctctcctc ttcaacctgg gcttcgtgtg tgatgcccag gccaggacct gcgccctcga
     4441 gcccattgtt agaaggctgg ctggctatct gaccacacta gagctagaga gcagcttcgt
     4501 gtccatggag gagagcaggc agaggttggt gcccatcatg accatcttgc tggaggagct
     4561 aaatgcctca ggccggtgca ctctgcccat tgatgagtcc aacaccatcc acttgagggt
     4621 gattgagcag cggccagacc ctccggtggc ccaggagtat gatgtgcctg tctttaccag
     4681 agacagggag gatttcttca actcacagtg ggacctcact acacaacaaa tcctgcccta
     4741 cattgatggg ttccgccaca tccagaggat ttcagcagag gcagatgtgg agctcaacct
     4801 ggtgcgcatt gctatccaga acctgctgta ctacggcgtt gtgacactgg tgtccatcct
     4861 ccagtactcc aatgtatact gcccaacgcc cagggtccag gacctggtag atgacaggtc
     4921 cctgcaagag gcatgtctat cctacgtgac caggcaaggg cacaggaggg ccagtctccg
     4981 ggatgtgttc cagctatact gcagcctgag ccctggcact accgtgcgag acctcattgg
     5041 ccgccacccc cagcagctgc agcatgttga tgaacggagg ctgatccagt tcgggcttat
     5101 gaggaacctc atcaggcgac tacagaggta tcctgtgcgg gtgactcggg aagagcagag
     5161 ccaccctgcc cggctttata caggctgcca cagctatgac gagatctgct gcaggacagg
     5221 catgagctac catgagctgg atgagcggct tgaaaatgac cccaacatca tcatctgctg
     5281 gaggtgagcg gccgcagtga gaattctcga cctcgagaca aatggcagta ttcatccaca
     5341 attttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa
     5401 tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaattttc
     5461 gggtttatta cagggacagc agagatccac tttggccgcg gctcgagggg gttggggttg
     5521 cgccttttcc aaggcagccc tgggtttgcg cagggacgcg gctgctctgg gcgtggttcc
     5581 gggaaacgca gcggcgccga ccctgggtct cgcacattct tcacgtccgt tcgcagcgtc
     5641 acccggatct tcgccgctac ccttgtgggc cccccggcga cgcttcctgc tccgccccta
     5701 agtcgggaag gttccttgcg gttcgcggcg tgccggacgt gacaaacgga agccgcacgt
     5761 ctcactagta ccctcgcaga cggacagcgc cagggagcaa tggcagcgcg ccgaccgcga
     5821 tgggctgtgg ccaatagcgg ctgctcagca gggcgcgccg agagcagcgg ccgggaaggg
     5881 gcggtgcggg aggcggggtg tggggcggta gtgtgggccc tgttcctgcc cgcgcggtgt
     5941 tccgcattct gcaagcctcc ggagcgcacg tcggcagtcg gctccctcgt tgaccgaatc
     6001 accgacctct ctccccaggg ggatccaccg gagcttacca tgaccgagta caagcccacg
     6061 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     6121 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     6181 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     6241 gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     6301 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     6361 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     6421 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     6481 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     6541 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     6601 ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgac gcccgcccca cgacccgcag
     6661 cgcccgaccg aaaggagcgc acgaccccat gcatcggtac ctttaagacc aatgacttac
     6721 aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt
     6781 cactcccaac gaagacaaga tctgcttttt gcttgtactg ggtctctctg gttagaccag
     6841 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
     6901 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
     6961 tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt
     7021 attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag gaacttgttt
     7081 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     7141 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
     7201 tggctctagc tatcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
     7261 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
     7321 cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta gggacgtacc
     7381 caattcgccc tatagtgagt cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg
     7441 tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc
     7501 cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct
     7561 gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
     7621 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
     7681 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
     7741 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg
     7801 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac
     7861 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta
     7921 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat
     7981 ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt aggtggcact
     8041 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     8101 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     8161 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     8221 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     8281 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     8341 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     8401 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     8461 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     8521 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     8581 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     8641 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     8701 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     8761 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     8821 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     8881 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     8941 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     9001 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     9061 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     9121 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     9181 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     9241 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     9301 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     9361 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     9421 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     9481 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     9541 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     9601 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     9661 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     9721 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     9781 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     9841 ttctttcctg cgttatcccc tgattctgtg gat
//
