LOCUS       sequence-376970-        8739 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For knocking p2a-tdTomato into FGF8 locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8739)
  AUTHORS   Xu P, He H, Gao Q, Zhou Y, Wu Z, Zhang X, Sun L, Hu G, Guan Q, You 
            Z, Zhang X, Zheng W, Xiong M, Chen Y
  TITLE     Human midbrain dopaminergic neuronal differentiation markers predict
            cell therapy outcomes in a Parkinson's disease model.
  JOURNAL   J Clin Invest. 2022 Jul 15;132(14). pii: 156768. doi: 
            10.1172/JCI156768.
  PUBMED    35700056
REFERENCE   2  (bases 1 to 8739)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Clin 
            Invest."; date: "2022-07-15"; volume: "132(14). pii: 156768. doi"; 
            pages: " 10.1172/JCI156768"
COMMENT     Sequence Label: PL452-FGF8-LA-p2a-tdTomato-hGHpA-PGK-neoR-FGF8-RA
FEATURES             Location/Qualifiers
     source          1..8739
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(16..444)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(126..147)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     338..357
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        471..575
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(576..644,645..1436)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(794..813)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1607..2195
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2096..2115
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2349..2366
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2483..2504
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2519..2524,2525..2542,2543..2549)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2557..2573
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2562..2584
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2581..2597
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2581..2597
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        2618..2636
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             3643..3699
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             3700..5130
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     protein_bind    5164..5197
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    5219..5695
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(5418..5437)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     promoter        5756..6255
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5791..5814)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6188..6207
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     promoter        6267..6314
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6333..7136
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6396..6415)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7006..7025
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(7168..7185)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7174..7398
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    7455..7488
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(8545..8564)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8546..8564)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8571..8588)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8571..8587)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8580..8602)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat
       61 aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg
      121 ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc
      181 gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt
      241 tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag
      301 cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg
      361 gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc
      421 ttaatgcgcc gctacagggc gcgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
      481 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
      541 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
      601 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
      661 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
      721 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
      781 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
      841 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
      901 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
      961 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     1021 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     1081 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     1141 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     1201 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     1261 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     1321 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     1381 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     1441 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     1501 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     1561 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     1621 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     1681 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     1741 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     1801 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     1861 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     1921 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     1981 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     2041 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     2101 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     2161 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     2221 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     2281 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     2341 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
     2401 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
     2461 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
     2521 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
     2581 caggaaacag ctatgaccat gattacgcca agctcgaaat taaccctcac taaagggaac
     2641 aaaagctggt accagtgagg gagaaggaag ctgtttgaga gatgaaaact ataaaaggcg
     2701 aaggaggaag aatgaaggct agggaagggg ctggggggtg gtggttggta aattccatat
     2761 atctttttaa caaatcgcca aattgctttg ctattatgtc caattacatg gtaccagcat
     2821 ttggaatgtt cagtaagccg caggcccagc ccctcggccg agctctgaaa tggctccatt
     2881 agtcacggct cctctccagc ctcgagctcc ccacttcctg ggcttctggg gctggggtct
     2941 tagcatcttc tcccaggcct cccctccccc atagggtggc tgccctgggg ccagggaacc
     3001 gaagtcctgg gggggtgaga ggggcaggtg gggagacggg tggccagact ggtgggcagg
     3061 aggccagagc aggccaggct ctgggcccct ctctctgtct ttctgcgttg gggcccagcc
     3121 cctccgtaga caaccatgtg tcactgctgc ctgggaggac aggaagttgc cgggtgggct
     3181 gcgagttgtg agggattaga gagcgggtgc ccaggcaggg ggtggggctg cggctcctgc
     3241 ccacctcgcc atctgctggg gtgcccacct gctgtctggg gccgctcgcc ctctgcctct
     3301 gctggggggg ctctgtaacg tggtgtctgg ctcccctacc tgcagagcaa cggcaaaggc
     3361 aaggactgcg tcttcacgga gattgtgctg gagaacaact acacagcgct gcagaatgcc
     3421 aagtacgagg gctggtacat ggccttcacc cgcaagggcc ggccccgcaa gggctccaag
     3481 acgcggcagc accagcgtga ggtccacttc atgaagcggc tgccccgggg ccaccacacc
     3541 accgagcaga gcctgcgctt cgagttcctc aactacccgc ccttcacgcg cagcctgcgc
     3601 ggcagccaga ggacttgggc ccccgagccc cgaggatccg gcgcaacaaa cttctctctg
     3661 ctgaaacaag ccggagatgt cgaagagaat cctggaccga tggtgagcaa gggcgaggag
     3721 gtcatcaaag agttcatgcg cttcaaggtg cgcatggagg gctccatgaa cggccacgag
     3781 ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg
     3841 aaggtgacca agggcggccc cctgcccttc gcctgggaca tcctgtcccc ccagttcatg
     3901 tacggctcca aggcgtacgt gaagcacccc gccgacatcc ccgattacaa gaagctgtcc
     3961 ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg tctggtgacc
     4021 gtgacccagg actcctccct gcaggacggc acgctgatct acaaggtgaa gatgcgcggc
     4081 accaacttcc cccccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc
     4141 accgagcgcc tgtacccccg cgacggcgtg ctgaagggcg agatccacca ggccctgaag
     4201 ctgaaggacg gcggccacta cctggtggag ttcaagacca tctacatggc caagaagccc
     4261 gtgcaactgc ccggctacta ctacgtggac accaagctgg acatcacctc ccacaacgag
     4321 gactacacca tcgtggaaca gtacgagcgc tccgagggcc gccaccacct gttcctgggg
     4381 catggcaccg gcagcaccgg cagcggcagc tccggcaccg cctcctccga ggacaacaac
     4441 atggccgtca tcaaagagtt catgcgcttc aaggtgcgca tggagggctc catgaacggc
     4501 cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc
     4561 aagctgaagg tgaccaaggg cggccccctg cccttcgcct gggacatcct gtccccccag
     4621 ttcatgtacg gctccaaggc gtacgtgaag caccccgccg acatccccga ttacaagaag
     4681 ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggtctg
     4741 gtgaccgtga cccaggactc ctccctgcag gacggcacgc tgatctacaa ggtgaagatg
     4801 cgcggcacca acttcccccc cgacggcccc gtaatgcaga agaagaccat gggctgggag
     4861 gcctccaccg agcgcctgta cccccgcgac ggcgtgctga agggcgagat ccaccaggcc
     4921 ctgaagctga aggacggcgg ccactacctg gtggagttca agaccatcta catggccaag
     4981 aagcccgtgc aactgcccgg ctactactac gtggacacca agctggacat cacctcccac
     5041 aacgaggact acaccatcgt ggaacagtac gagcgctccg agggccgcca ccacctgttc
     5101 ctgtacggca tggacgagct gtacaagtaa tgagaattcc gatcatattc aataaccctt
     5161 aatataactt cgtataatgt atgctatacg aagttatagc gctgctcgag agatctacgg
     5221 gtggcatccc tgtgacccct ccccagtgcc tctcctggcc ctggaagttg ccactccagt
     5281 gcccaccagc cttgtcctaa taaaattaag ttgcatcatt ttgtctgact aggtgtcctt
     5341 ctataatatt atggggtgga ggggggtggt atggagcaag gggcaagttg ggaagacaac
     5401 ctgtagggcc tgcggggtct attgggaacc aagctggagt gcagtggcac aatcttggct
     5461 cactgcaatc tccgcctcct gggttcaagc gattctcctg cctcagcctc ccgagttgtt
     5521 gggattccag gcatgcatga ccaggctcag ctaatttttg tttttttggt agagacgggg
     5581 tttcaccata ttggccaggc tggtctccaa ctcctaatct caggtgatct acccaccttg
     5641 gcctcccaaa ttgctgggat tacaggcgtg aaccactgct cccttccctg tcctttaggt
     5701 ctgaagagga gtttacgtcc agccacgcgt aggatctcga cctcgaaatt ctaccgggta
     5761 ggggaggcgc ttttcccaag gcagtctgga gcatgcgctt tagcagcccc gctgggcact
     5821 tggcgctaca caagtggcct ctggcctcgc acacattcca catccaccgg taggcgccaa
     5881 ccgactccgt tctttggtgg ccccttcgcg ccaccttcta ctcctcccct agtcaggaag
     5941 ttcccccccg ccccgcagct cgcgtcgtgc aggacgtgac aaatggaagt agcacgtctc
     6001 actagtctcg tgcagatgga cagcaccgct gagcaatgga agcgggtagg cctttggggc
     6061 agcggccaat agcagctttg ctccttcgct ttctgggctc agaggctggg aaggggtggg
     6121 tccgggggcg ggctcagggg cgggctcagg ggcggggcgg gcgcccgaag gtcctccgga
     6181 ggcccggcat tctgcacgct tcaaaagcgc acgtctgccg cgctgttctc ctcttcctca
     6241 tctccgggcc tttcgacctg cagcctgttg acaattaatc atcggcatag tatatcggca
     6301 tagtataata cgacaaggtg aggaactaaa ccatgggatc ggccattgaa caagatggat
     6361 tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac
     6421 agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc
     6481 tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaggacgag gcagcgcggc
     6541 tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag
     6601 cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc
     6661 ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg
     6721 atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc
     6781 ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc
     6841 cagccgaact gttcgccagg ctcaaggcgc gcatgcccga cggcgatgat ctcgtcgtga
     6901 cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca
     6961 tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg
     7021 atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg
     7081 ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagggg
     7141 atcaattctc tagagctcgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg
     7201 ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt
     7261 cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg
     7321 gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg
     7381 atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctcg actagagctt
     7441 gcggaaccct taatataact tcgtataatg tatgctatac gaagttatta ggtccctcga
     7501 ggggatcctg agtcttaggt gctgccttta ccgaaacaca atgccagacc gcagagaggc
     7561 tcatcggtga gggcacccaa aactcaagca agatgagctg tgcgctgctc tgcaggctgg
     7621 ggaggtgctg ggggagccct gggttccggt tgttgatatt gtttgctgtt gggtttttgc
     7681 tgtttttttt tttttttttt ttttaaaaca aaagagaggc tctatttttg tattccactt
     7741 ggctgtggtg tctgtcttct taactctcag aaagctccat tagtggccta gactgggatt
     7801 ccggctgggg gtttgcgggg gtggggggct ttctctagcc tgtgctgctg aggccccagt
     7861 acctccaggg ccagttggct gggcagccag ggactccact gcacccccag gtggggcagg
     7921 gaggaaagga ctgtgacata gggcagtcct cttagaagtg ggtatcagac tggtggctat
     7981 taaatgattg aaatatttat ttaacttgca tattaaaaat gtgtgctgga gagtgagtcc
     8041 tgccggggtc agcccctccc tccaaccttg ccccagctgg tgggcggctg ggagacgcag
     8101 atgaccaggt gccagctctg accacagcct ccctccagcc taaagacacc tgcctgtcaa
     8161 ccatccccat cactgtcact tgaggggttt tcctgcaagg acagaagcag ggaaaggggc
     8221 aagaagaggc tcttagctag tccttggagc tctcagatgt gtacctccta gcactttaca
     8281 gaggtcattg ctaacacttc ccaggccacc tcagggccag aaataatgga tgtgctaggg
     8341 ctagagctgt aatcatggat ttaatcctct taaaaagtgc ttctctgagt gcctaggtcc
     8401 atgtgggaga caggttggag attccagaac ttgctctttc tgagactcag gctccagaaa
     8461 atgaaagaaa agagcagctg ccagggtcca aggtgggggc atattggagg ggggcggccg
     8521 ccaccgcggt ggagctccaa ttcgccctat agtgagtcgt attacaattc actggccgtc
     8581 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca
     8641 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
     8701 cagttgcgca gcctgaatgg cgaatggaaa ttgtaagcg
//
