LOCUS       sequence-376975-        9107 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CMV-driven expression of an untagged mutant NPRL3 (core subunit of 
            GATOR1) — codon-optimized sequence for stable expression in 
            mammalian cells. All lysine residues were mutated to arginines..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9107)
  AUTHORS   Valenstein ML, Rogala KB, Lalgudi PV, Brignole EJ, Gu X, Saxton RA, 
            Chantranupong L, Kolibius J, Quast JP, Sabatini DM
  TITLE     Structure of the nutrient-sensing hub GATOR2.
  JOURNAL   Nature. 2022 Jul;607(7919):610-616. doi: 10.1038/s41586-022-04939-z.
            Epub 2022 Jul 13.
  PUBMED    35831510
REFERENCE   2  (bases 1 to 9107)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-022-04939-z"; journalName: "Nature"; date: 
            "2022-07"; volume: "607"; issue: "7919"; pages: "610-616"
COMMENT     Sequence Label: K-to-R mutant NPRL3(1-569) in pLJM60,
            codon-op...
FEATURES             Location/Qualifiers
     source          1..9107
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      20..475
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(107..126)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     317..338
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        501..605
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(606..674,675..1466)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(824..843)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1637..2225
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2126..2145
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2379..2396
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2513..2534
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2549..2554,2555..2572,2573..2579)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2587..2603
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2592..2614
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2611..2627
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2611..2627
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2646..2666
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2648..2666
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2694..2920
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2921..3101
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    3148..3273
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3770..4003
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             4188..4232
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        4539..4742
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4693..4713
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     4739..4763
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        4973..4991
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     4973..4990
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     5004..5023
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             5082..5105
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    6894..7011
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        7060..7570
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     7489..7507
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             7592..8191
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7592..7611)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8088..8108
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             8319..8552
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8624..8745
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8661..8680)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8715..8734
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8785..8920
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8940..8959)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8941..8959)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8969..8986)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8969..8985)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8978..9000)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 agcctgaatg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
       61 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
      121 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
      181 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
      241 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
      301 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
      361 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
      421 ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttaggtg
      481 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
      541 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
      601 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
      661 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
      721 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
      781 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
      841 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
      901 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
      961 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     1021 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     1081 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     1141 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     1201 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     1261 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     1321 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     1381 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     1441 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     1501 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     1561 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     1621 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     1681 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     1741 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     1801 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     1861 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     1921 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     1981 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     2041 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     2101 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     2161 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     2221 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     2281 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     2341 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     2401 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
     2461 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
     2521 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt
     2581 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca
     2641 agcgcgcaat taaccctcac taaagggaac aaaagctgga gctgcaagct taatgtagtc
     2701 ttatgcaata ctcttgtagt cttgcaacat ggtaacgatg agttagcaac atgccttaca
     2761 aggagagaaa aagcaccgtg catgccgatt ggtggaagta aggtggtacg atcgtgcctt
     2821 attaggaagg caacagacgg gtctgacatg gattggacga accactgaat tgccgcattg
     2881 cagagatatt gtatttaagt gcctagctcg atacataaac gggtctctct ggttagacca
     2941 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     3001 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     3061 atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac
     3121 ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg
     3181 cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga
     3241 ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg
     3301 cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata
     3361 gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca
     3421 gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa
     3481 cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata
     3541 aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc
     3601 gcacagcaag cggccggccg ctgatcttca gacctggagg aggagatatg agggacaatt
     3661 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca
     3721 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt
     3781 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg
     3841 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta
     3901 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa
     3961 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct
     4021 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc
     4081 tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca
     4141 caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag
     4201 aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc
     4261 tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt
     4321 ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga
     4381 cccacctccc aaccccgagg ggaccccata tgccaagtac gccccctatt gacgtcaatg
     4441 acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt
     4501 ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca
     4561 tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg
     4621 tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact
     4681 ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag
     4741 ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt gacctccata
     4801 gaagacaccg ggaccgatcc agcctccgcg gccgggaacg gtgcattgga acgcggattc
     4861 cccgtgccaa gagtgacgta agtaccgcct atagagtcta taggcccacc cccttggctt
     4921 cgttagaacg cggctacaat taatacataa ccttatgtat catacacata cgatttaggt
     4981 gacactatag aataacatcc actttgcctt tctctccaca ggtgtccact cccaggtcca
     5041 actgcacctc ggttctatcg attgaattca ccatggaaat ggactacaag gatgacgatg
     5101 acaagggtgg tggtggtggt gcgtcgacga tgcgggataa cacttcccct attagcgtta
     5161 tcctcgtctc ttcaggctcc cgagggaata ggctcctgtt tcgctaccca tttcaaagaa
     5221 gtcaggaaca tccagcctca caaacaagcc ggccaagatc acggtatgcg gcaagtaaca
     5281 ccggggacca cgccgacgag caagatggag atagtcgctt ttcagatgtc atactggcaa
     5341 caatcctcgc aacgcggtcc gagatgtgtg ggcagcggtt cgaactgcga atagacaacg
     5401 taagattcgt gggacatccg acccttttgc agcacgcact cggccaaatc tcaaggactg
     5461 atccatcccc gcgacgggag gctccgacta tgatactctt taatgtcgtg ttcgccctgc
     5521 gggccaacgc ggacccatct gtcatcaact gtctgcacaa tctctcccga aggatcgcga
     5581 cagtcctcca gcatgaagaa cgacggtgtc aatacttgac cagagaagcc cggttgatcc
     5641 ttgcgctgca agatgaagtg tctgccatgg cagatggtaa cgaaggcccg cagtccccat
     5701 ttcatcatat tctcccgcgc tgccgcttgg ccagggatct gcgagaggcc tacgattcat
     5761 tgtgcactag cggcgtagtg agactccaca taaactcctg gctcgaggtc tcattctgtc
     5821 tcccgcatcg gatccactac gcggcttcct cactcatccc acccgaagcc attgagcgga
     5881 gtctcagagc aatacgaccc tatcatgcac tgttgcttct gtccgatgag agaagtctgt
     5941 tgggcgaact gcccattgac tgtagccctg ccttggtccg agtaatacga accacttccg
     6001 cggttaggaa tctgcagcag cttgctcagg atgcggatct ggcattgctt caagtcttcc
     6061 aactcgcagc ccatctcgtg tattggggac gcgccattat aatctacccg ctttgcgaaa
     6121 acaacgtcta tatgctgagc cctaacgcca gcgtatgtct gtactctccc ctggctgaac
     6181 agttctctca tcaatttcct agtcatgatc ttccgagtgt cctcgcgaga ttttctctcc
     6241 ctgtttcact ctccgaattc aggaacccac tggcgccagc tgttcaagaa acacagctta
     6301 tacagatggt tgtgtggatg ctgcaacgga ggctgctcat acagttgcat acctatgttt
     6361 gcctgatggc ttcaccctct gaggaagaac cccgcccacg cgaagatgac gtgcccttca
     6421 cggcccgcgt aggaggtagg tcactgagca cgccaaatgc tttgtcattt ggcagcccca
     6481 cttcttccga tgatatgact cttacctccc cttctatgga caatagctcc gcagaacttt
     6541 tgccgtccgg ggattccccg ctcaaccaga gaatgaccga gaatctgctt gcgtccttgt
     6601 ccgaacacga acgagctgcg attctctctg tccctgccgc ccagaatccc gaagatctgc
     6661 gcatgtttgc cagactgttg cattatttta gggggaggca ccatctggag gaaataatgt
     6721 ataacgaaaa tacgcgaaga agtcaacttc ttatgctttt cgatagattt aggtcagttc
     6781 ttgtggtaac tacacatgag gatccagtca tcgcagtatt ccaagctctc ctgccctgag
     6841 cggccgcagt gagaattctc gacctcgaga caaatggcag tattcatcca caattttaaa
     6901 agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca
     6961 gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat
     7021 tacagggaca gcagagatcc actttggccg cggctcgagg gggttggggt tgcgcctttt
     7081 ccaaggcagc cctgggtttg cgcagggacg cggctgctct gggcgtggtt ccgggaaacg
     7141 cagcggcgcc gaccctgggt ctcgcacatt cttcacgtcc gttcgcagcg tcacccggat
     7201 cttcgccgct acccttgtgg gccccccggc gacgcttcct gctccgcccc taagtcggga
     7261 aggttccttg cggttcgcgg cgtgccggac gtgacaaacg gaagccgcac gtctcactag
     7321 taccctcgca gacggacagc gccagggagc aatggcagcg cgccgaccgc gatgggctgt
     7381 ggccaatagc ggctgctcag cagggcgcgc cgagagcagc ggccgggaag gggcggtgcg
     7441 ggaggcgggg tgtggggcgg tagtgtgggc cctgttcctg cccgcgcggt gttccgcatt
     7501 ctgcaagcct ccggagcgca cgtcggcagt cggctccctc gttgaccgaa tcaccgacct
     7561 ctctccccag ggggatccac cggagcttac catgaccgag tacaagccca cggtgcgcct
     7621 cgccacccgc gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt tcgccgacta
     7681 ccccgccacg cgccacaccg tcgatccgga ccgccacatc gagcgggtca ccgagctgca
     7741 agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg
     7801 cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga
     7861 gatcggcccg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga
     7921 aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt
     7981 ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc
     8041 ggccgagcgc gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt
     8101 ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac
     8161 ctggtgcatg acccgcaagc ccggtgcctg acgcccgccc cacgacccgc agcgcccgac
     8221 cgaaaggagc gcacgacccc atgcatcggt acctttaaga ccaatgactt acaaggcagc
     8281 tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca
     8341 acgaagacaa gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc
     8401 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
     8461 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
     8521 acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca
     8581 gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc
     8641 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     8701 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta
     8761 gctatcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
     8821 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct
     8881 gagctattcc agaagtagtg aggaggcttt tttggaggcc tagggacgta cccaattcgc
     8941 cctatagtga gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg
     9001 aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc
     9061 gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgc
//
