LOCUS       sequence-376979-        9963 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral construct that expresses a dox-inducible human PPARG2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9963)
  AUTHORS   Friesen M, Khalil AS, Barrasa MI, Jeppesen JF, Mooney DJ, Jaenisch R
  TITLE     Development of a physiological insulin resistance model in human 
            stem cell-derived adipocytes.
  JOURNAL   Sci Adv. 2022 Jun 17;8(24):eabn7298. doi: 10.1126/sciadv.abn7298. 
            Epub 2022 Jun 17.
  PUBMED    35714187
REFERENCE   2  (bases 1 to 9963)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2022-06-17"; volume: "8(24):eabn7298. doi"; pages: " 
            10.1126/sciadv.abn7298. Epub 2022 Jun 17"
COMMENT     Sequence Label: pMF-FU_TetO_GW_PPARG2
FEATURES             Location/Qualifiers
     source          1..9963
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    40..628
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(93..113)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(511..522)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(631..647)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(632..648)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             1153..1333
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(1359..1376)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    1365..1589
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      1635..2063
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1722..1741)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1932..1953
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(2072..2092)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2077..2406
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2257..2392
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     2319..2338
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        2454..2501
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             2520..2894
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    3024..3145
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3061..3080)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3115..3134
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3194..3210)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3194..3210)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3207..3229)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3218..3234
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3242..3248,3249..3266,3267..3272))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3287..3308
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3425..3442)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3596..4184)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3676..3695)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4355..5146,5147..5215))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4978..4997
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5216..5320)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(5395..5414)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        5586..5965
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5967..6165
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             6183..6363
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6410..6535
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7028..7261
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7446..7490
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7788..7905
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(7958..7976,7977..7999,8000..8018,8019..8041,8042..8060,
                     8061..8083,8084..8102,8103..8125,8126..8144,8145..8167,
                     8168..8186,8187..8209,8210..8228)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        8261..8299
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     8296..8320
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    8401..8425
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(join(9953..9963,1..14))
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ttgtacaaag tggtgatatt cgatatcaag cttatcgata atcaacctct ggattacaaa
       61 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
      121 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
      181 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt
      241 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc
      301 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc
      361 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
      421 gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt
      481 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc
      541 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt
      601 cggatctccc tttgggccgc ctccccgcat cgataccgtc gacctcgaga cctagaaaaa
      661 catggagcaa tcacaagtag caatacagca gctaccaatg ctgattgtgc ctggctagaa
      721 gcacaagagg aggaggaggt gggttttcca gtcacacctc aggtaccttt aagaccaatg
      781 acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg
      841 ctaattcact cccaacgaag acaagatatc cttgatctgt ggatctacca cacacaaggc
      901 tacttccctg attggcagaa ctacacacca gggccaggga tcagatatcc actgaccttt
      961 ggatggtgct acaagctagt accagttgag caagagaagg tagaagaagc caatgaagga
     1021 gagaacaccc gcttgttaca ccctgtgagc ctgcatggga tggatgaccc ggagagagaa
     1081 gtattagagt ggaggtttga cagccgccta gcatttcatc acatggcccg agagctgcat
     1141 ccggactgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     1201 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     1261 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     1321 aaaatctcta gcagggcccg tttaaacccg ctgatcagcc tcgactgtgc cttctagttg
     1381 ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc
     1441 cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc
     1501 tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag
     1561 gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc
     1621 tagggggtat ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
     1681 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
     1741 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
     1801 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg
     1861 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac
     1921 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta
     1981 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat
     2041 ttaacaaaaa tttaacgcga attaattctg tggaatgtgt gtcagttagg gtgtggaaag
     2101 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     2161 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     2221 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     2281 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     2341 gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
     2401 tgcaaaaagc tcccgggagc ttgtatatcc attttcggat ctgatcagca cgtgttgaca
     2461 attaatcatc ggcatagtat atcggcatag tataatacga caaggtgagg aactaaacca
     2521 tggccaagtt gaccagtgcc gttccggtgc tcaccgcgcg cgacgtcgcc ggagcggtcg
     2581 agttctggac cgaccggctc gggttctccc gggacttcgt ggaggacgac ttcgccggtg
     2641 tggtccggga cgacgtgacc ctgttcatca gcgcggtcca ggaccaggtg gtgccggaca
     2701 acaccctggc ctgggtgtgg gtgcgcggcc tggacgagct gtacgccgag tggtcggagg
     2761 tcgtgtccac gaacttccgg gacgcctccg ggccggccat gaccgagatc ggcgagcagc
     2821 cgtgggggcg ggagttcgcc ctgcgcgacc cggccggcaa ctgcgtgcac ttcgtggccg
     2881 aggagcagga ctgacacgtg ctacgagatt tcgattccac cgccgccttc tatgaaaggt
     2941 tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca
     3001 tgctggagtt cttcgcccac cccaacttgt ttattgcagc ttataatggt tacaaataaa
     3061 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     3121 tgtccaaact catcaatgta tcttatcatg tctgtatacc gtcgacctct agctagagct
     3181 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
     3241 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
     3301 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
     3361 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
     3421 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     3481 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     3541 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     3601 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     3661 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     3721 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     3781 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     3841 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     3901 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     3961 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     4021 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     4081 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     4141 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     4201 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     4261 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     4321 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     4381 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     4441 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     4501 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     4561 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     4621 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     4681 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     4741 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     4801 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     4861 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     4921 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     4981 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     5041 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     5101 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     5161 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     5221 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     5281 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     5341 cacctgacgt cgacggatcg ggagatctcc cgatccccta tggtgcactc tcagtacaat
     5401 ctgctctgat gccgcatagt taagccagta tctgctccct gcttgtgtgt tggaggtcgc
     5461 tgagtagtgc gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg acaattgcat
     5521 gaagaatctg cttagggtta ggcgttttgc gctgcttcgc gatgtacggg ccagatatac
     5581 gcgttgacat tgattattga ctagttatta atagtaatca attacggggt cattagttca
     5641 tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc
     5701 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat
     5761 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
     5821 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc
     5881 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta
     5941 cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg
     6001 atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt
     6061 gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac
     6121 gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagcgcgt tttgcctgta
     6181 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
     6241 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
     6301 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     6361 gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg
     6421 caggactcgg cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac
     6481 gccaaaaatt ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat
     6541 taagcggggg agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa
     6601 aaaatataaa ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa
     6661 tcctggcctg ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc
     6721 ccttcagaca ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg
     6781 tgtgcatcaa aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga
     6841 gcaaaacaaa agtaagacca ccgcacagca agcggccgct gatcttcaga cctggaggag
     6901 gagatatgag ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac
     6961 cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag
     7021 tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag
     7081 cgtcaatgac gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga
     7141 acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca
     7201 tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc
     7261 tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta
     7321 gttggagtaa taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca
     7381 gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc
     7441 aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt
     7501 ttaacataac aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg
     7561 taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt
     7621 caccattatc gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa
     7681 tagaagaaga aggtggagag agagacagag acagatccat tcgattagtg aacggatcgg
     7741 cactgcgtgc gccaattctg cagacaaatg gcagtattca tccacaattt taaaagaaaa
     7801 ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata
     7861 caaactaaag aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg
     7921 gacagcagag atccagtttg gttatcgagt ttaccactcc ctatcagtga tagagaaaag
     7981 tgaaagtcga gtttaccact ccctatcagt gatagagaaa agtgaaagtc gagtttacca
     8041 ctccctatca gtgatagaga aaagtgaaag tcgagtttac cactccctat cagtgataga
     8101 gaaaagtgaa agtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt
     8161 ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gtttaccact ccctatcagt
     8221 gatagagaaa agtgaaagtc gagctcggta cccgggtcga ggtaggcgtg tacggtggga
     8281 ggcctatata agcagagctc gtttagtgaa ccgtcagatc gcctggagac gccatccacg
     8341 ctgttttgac ctccatagaa gacaccggga ccgatccagc ctccgcggcc ccgaattatc
     8401 acaagtttgt acaaaaaagc aggcttacca ccatgggtga aactctggga gattctccta
     8461 ttgacccaga aagcgattcc ttcactgata cactgtctgc aaacatatca caagaaatga
     8521 ccatggttga cacagagatg ccattctggc ccaccaactt tgggatcagc tccgtggatc
     8581 tctccgtaat ggaagaccac tcccactcct ttgatatcaa gcccttcact actgttgact
     8641 tctccagcat ttctactcca cattacgaag acattccatt cacaagaaca gatccagtgg
     8701 ttgcagatta caagtatgac ctgaaacttc aagagtacca aagtgcaatc aaagtggagc
     8761 ctgcatctcc accttattat tctgagaaga ctcagctcta caataagcct catgaagagc
     8821 cttccaactc cctcatggca attgaatgtc gtgtctgtgg agataaagct tctggatttc
     8881 actatggagt tcatgcttgt gaaggatgca agggtttctt ccggagaaca atcagattga
     8941 agcttatcta tgacagatgt gatcttaact gtcggatcca caaaaaaagt agaaataaat
     9001 gtcagtactg tcggtttcag aaatgccttg cagtggggat gtctcataat gccatcaggt
     9061 ttgggcggat gccacaggcc gagaaggaga agctgttggc ggagatctcc agtgatatcg
     9121 accagctgaa tccagagtcc gctgacctcc gggccctggc aaaacatttg tatgactcat
     9181 acataaagtc cttcccgctg accaaagcaa aggcgagggc gatcttgaca ggaaagacaa
     9241 cagacaaatc accattcgtt atctatgaca tgaattcctt aatgatggga gaagataaaa
     9301 tcaagttcaa acacatcacc cccctgcagg agcagagcaa agaggtggcc atccgcatct
     9361 ttcagggctg ccagtttcgc tccgtggagg ctgtgcagga gatcacagag tatgccaaaa
     9421 gcattcctgg ttttgtaaat cttgacttga acgaccaagt aactctcctc aaatatggag
     9481 tccacgagat catttacaca atgctggcct ccttgatgaa taaagatggg gttctcatat
     9541 ccgagggcca aggcttcatg acaagggagt ttctaaagag cctgcgaaag ccttttggtg
     9601 actttatgga gcccaagttt gagtttgctg tgaagttcaa tgcactggaa ttagatgaca
     9661 gcgacttggc aatatttatt gccgtcatta ttctcagtgg agaccgccca ggtttgctga
     9721 atgtgaagcc cattgaagac attcaagaca acctgctaca agccctggag ctccagctga
     9781 agctgaacca ccctgagtcc tcacagctgt ttgccaagct gctccagaaa atgacagacc
     9841 tcagacagat tgtcacggaa cacgtgcagc tactgcaggt gatcaagaag acggagacag
     9901 acatgagtct tcacccgctc ctgcaggaga tctacaagga cttgtactag ctacccagct
     9961 ttc
//
