LOCUS       sequence-376982-        7804 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Barcoded piggybac transposon vector with Dox-inducible expression of
            MYC.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7804)
  AUTHORS   Pierson Smela MD, Kramme CC, Fortuna PRJ, Adams JL, Su R, Dong E, 
            Kobayashi M, Brixi G, Kavirayuni VS, Tysinger E, Kohman RE, Shioda 
            T, Chatterjee P, Church GM
  TITLE     Directed differentiation of human iPSCs to functional ovarian 
            granulosa-like cells via transcription factor overexpression.
  JOURNAL   Elife. 2023 Feb 21;12:e83291. doi: 10.7554/eLife.83291.
  PUBMED    36803359
REFERENCE   2  (bases 1 to 7804)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2023-02-21"; volume: "12:e83291. doi"; pages: " 
            10.7554/eLife.83291"
COMMENT     Sequence Label: pPB-cT3G-cERP2-MYC
FEATURES             Location/Qualifiers
     source          1..7804
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     21..45
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    111..135
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             141..1457
                     /codon_start=1
                     /product="human c-Myc proto-oncogene"
                     /label=c-Myc
                     /translation="MPLNVSFTNRNYDLDYDSVQPYFYCDEEENFYQQQQQSELQPPAP
                     SEDIWKKFELLPTPPLSPSRRSGLCSPSYVAVTPFSLRGDNDGGGGSFSTADQLEMVTE
                     LLGGDMVNQSFICDPDDETFIKNIIIQDCMWSGFSAAAKLVSEKLASYQAARKDSGSPN
                     PARGHSVCSTSSLYLQDLSAAASECIDPSVVFPYPLNDSSSPKSCASQDSSAFSPSSDS
                     LLSSTESSPQGSPEPLVLHEETPPTTSSDSEEEQEDEEEIDVVSVEKRQAPGKRSESGS
                     PSAGGHSKPPHSPLVLKRCHVSTHQHNYAAPPSTRKDYPAAKRVKLDSVRVLRQISNNR
                     KCTSPRSSDTEENVKRRTHNVLERQRRNELKRSFFALRDQIPELENNEKAPKVVILKKA
                     TAYILSVQAEEQKLISEEDLLRKRREQLKHKLEQLRNSCA"
     protein_bind    complement(1462..1486)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1488..1514
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    1681..1815
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     repeat_region   2087..2121
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(2132..2149)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2132..2148)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2141..2163)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(2357..2376)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2478..2500
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2539..2557)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2625..2729
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2730..2798,2799..3590)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2948..2967)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3761..4349
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4250..4269
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4503..4520
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4726..4747
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4762..4767,4768..4785,4786..4792)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4800..4816
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4805..4827
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4824..4840
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4824..4840
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     repeat_region   4866..4928
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     polyA_signal    5104..5185
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(5902..5958)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(5968..6711)
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     CDS             complement(6712..6735)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      6735..6744
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(6764..6975)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     enhancer        6976..7355
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     protein_bind    7564..7582
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7600..7618
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7636..7654
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7672..7690
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7708..7726
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7744..7762
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7780..7798
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 aagcttgcct atataagcag agctcgttta gtgaaccgtc agatcgcctg gagacgccat
       61 ccacgctgtt ttgacctcca tagaagacac cgggaccgat ccagcctacc acaagtttgt
      121 acaaaaaagc aggctccacc atgcccctca acgttagctt caccaacagg aactatgacc
      181 tcgactacga ctcggtgcag ccgtatttct actgcgacga ggaggagaac ttctaccagc
      241 agcagcagca gagcgagctg cagcccccgg cgcccagcga ggatatctgg aagaaattcg
      301 agctgctgcc caccccgccc ctgtccccta gccgccgctc cgggctctgc tcgccctcct
      361 acgttgcggt cacacccttc tcccttcggg gagacaacga cggcggtggc gggagcttct
      421 ccacggccga ccagctggag atggtgaccg agctgctggg aggagacatg gtgaaccaga
      481 gtttcatctg cgacccggac gacgagacct tcatcaaaaa catcatcatc caggactgta
      541 tgtggagcgg cttctcggcc gccgccaagc tcgtctcaga gaagctggcc tcctaccagg
      601 ctgcgcgcaa agacagcggc agcccgaacc ccgcccgcgg ccacagcgtc tgctccacct
      661 ccagcttgta cctgcaggat ctgagcgccg ccgcctcaga gtgcatcgac ccctcggtgg
      721 tcttccccta ccctctcaac gacagcagct cgcccaagtc ctgcgcctcg caagactcca
      781 gcgccttctc tccgtcctcg gattctctgc tctcctcgac ggagtcctcc ccgcagggca
      841 gccccgagcc cctggtgctc catgaggaga caccgcccac caccagcagc gactctgagg
      901 aggaacaaga agatgaggaa gaaatcgatg ttgtttctgt ggaaaagagg caggctcctg
      961 gcaaaaggtc agagtctgga tcaccttctg ctggaggcca cagcaaacct cctcacagcc
     1021 cactggtcct caagaggtgc cacgtctcca cacatcagca caactacgca gcgcctccct
     1081 ccactcggaa ggactatcct gctgccaaga gggtcaagtt ggacagtgtc agagtcctga
     1141 gacagatcag caacaaccga aaatgcacca gccccaggtc ctcggacacc gaggagaatg
     1201 tcaagaggcg aacacacaac gtcttggagc gccagaggag gaacgagcta aaacggagct
     1261 tttttgccct gcgtgaccag atcccggagt tggaaaacaa tgaaaaggcc cccaaggtag
     1321 ttatccttaa aaaagccaca gcatacatcc tgtccgtcca agcagaggag caaaagctca
     1381 tttctgaaga ggacttgttg cggaaacgac gagaacagtt gaaacacaaa cttgaacagc
     1441 tacggaactc ttgtgcgttg gacccagctt tcttgtacaa agtggtatac ccatacgatg
     1501 ttccagatta cgcttagtaa tgagaaatac tcagaagatg tcactcacca tagtccgcag
     1561 tgaaagataa atgatcaaat attgtggaac ccacttgttt tagagctaga aatagcaagt
     1621 aggccactga gcaagaacta tgaatgagat ctttagacat attgcggccg ctttctccgc
     1681 gatccagaca tgataagata cattgatgag tttggacaaa ccaaaactag aatgcagtga
     1741 aaaaaatgcc ttatttgtga aatttgtgat gctattgcct tatttgtaac cattataagc
     1801 tgcaataaac aagttatcta taacaagaaa atatatatat aataagttat cacgtaagta
     1861 gaacatgaaa taacaatata attatcgtat gagttaaatc ttaaaagtca cgtaaaagat
     1921 aatcatgcgt cattttgact cacgcggtcg ttatagttca aaatcagtga cacttaccgc
     1981 attgacaagc acgcctcacg ggagctccaa gcggcgactg agatgtccta aatgcacagc
     2041 gacggattcg cgctatttag aaagagagag caatatttca agaatgcatg cgtcaatttt
     2101 acgcagacta tctttctagg gttaagaatt cactggccgt cgttttacaa cgtcgtgact
     2161 gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct
     2221 ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg
     2281 gcgaatggcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca
     2341 tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc
     2401 cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac
     2461 aagctgtgac catgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa
     2521 acacgcgagt tcgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa
     2581 taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     2641 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     2701 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     2761 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     2821 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     2881 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     2941 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     3001 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     3061 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     3121 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     3181 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     3241 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     3301 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     3361 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     3421 ataaatctgg agccggtgag cgtggttcac gcggtatcat tgcagcactg gggccagatg
     3481 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     3541 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     3601 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     3661 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     3721 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     3781 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     3841 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     3901 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     3961 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     4021 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     4081 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     4141 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     4201 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     4261 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     4321 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     4381 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     4441 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     4501 gcagcgagtc agtgagcgag gaagcggatg agcgcccaat acgcaaaccg cctctcaccg
     4561 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca
     4621 gtgagcgcaa cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
     4681 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
     4741 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
     4801 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaaggtcga
     4861 cttaacccta gaaagataat catattgtga cgtacgttaa agataatcat gcgtaaaatt
     4921 gacgcatgtg ttttatcggt ctgtatatcg aggtttattt attaatttga atagatatta
     4981 agttttatta tatttacact tacatactaa taataaattc aacaaacaat ttatttatgt
     5041 ttatttattt attaaaaaaa aacaaaaact caaaatttct tctataaagt aacaaaactt
     5101 ttagcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac
     5161 cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt
     5221 tcagggggag gtgtgggagg ttttttaaag caagtaaaac ctctacaaat gtggtatggc
     5281 tgattatgat cctctggaga ttcatgcgcc aggttttctg gtcatgcacc acgttcgtgg
     5341 gccctcagga acctccacgt cagcggtaac tgtgaagccc agtcgctcgt aaaaaggaag
     5401 attccgggga gcactggttt cgaggaacgc cggcacgcca gcccgttccg cggcctcaac
     5461 accaggaaga acgacagcgc ttcccaaacc tttaccttgg tgatctgggc tgacaccgac
     5521 agtggcaaga aaccacgcag gctcctttgg tctatgagga gccagcaagc cctccatttg
     5581 ctgttgtgcc gccaacctac tccctgacag ctcggccatc cgcggaccta tttccgcgaa
     5641 cactgcgccg gcttcgacgc tttccggagt agtccagacc gcaactgcgg caccatcgtc
     5701 cgcaacccaa actttaccaa tgtccagacc cacgcgcgta agaaacagct cttgcaactc
     5761 agttaccctc tctatgtgtc tgtccggatc tacggtatgg cgggtcgctg gataatctgc
     5821 aaatgctgcg gcaagtgttc ggacagcccg tggtacatcg tcacgggtag cgagcctaac
     5881 agtgggctta tattccgtca tggggccggg gttctcctcc acgtcgccgg cctgcttcag
     5941 caggctgaag ttggtggcgc cgctgccccc ggggagcatg tcaaggtcaa aatcgtcaag
     6001 agcgtcagca ggcagcatat caaggtcaaa gtcgtcaagg gcatcggctg ggagcatgtc
     6061 taagtcaaaa tcgtcaaggg cgtcggtcgg cccgccgctt tcgcacttta gctgtttctc
     6121 caggccacat atgattagtt ccaggccgaa aaggaaggca ggttcggctc cctgccggtc
     6181 gaacagctca attgcttgtt tcagaagtgg gggcatagaa tcggtggtag gtgtctctct
     6241 ttcctctttt gctacttgat gctcctgttc ctccaatacg cagcccagtg taaagtggcc
     6301 cacggcggac agagcgtaca gtgcgttctc cagggagaag ccttgctgac acaggaacgc
     6361 gagctgattt tccagggttt cgtactgttt ctctgttggg cgggtgccga gatgcacttt
     6421 agccccgtca cgatgtgaga ggagagcaca gcggtatgac ttggcgttgt tccgcagaaa
     6481 gtcttgccat gactcgcctt ccagggggca ggagtgggta tgatgcctgt ccagcatctc
     6541 gattggcagg gcatcgagca gggcccgctt gttcttcacg tgccagtaca gggtaggctg
     6601 ctcaactccc agcttttgag cgagtttcct tgtcgtcagg ccttcgatac cgactccatt
     6661 gagtaattcc agagcagagt ttatgacttt gctcttgtcc agtctagaca tcttatcgtc
     6721 atcgtctttg taatccatgg tggcggatcc cgcgtcacga cacctgtgtt ctggcggcaa
     6781 acccgttgcg aaaaagaacg ttcacggcga ctactgcact tatatacggt tctcccccac
     6841 cctcgggaaa aaggcggagc cagtacacga catcactttc ccagtttacc ccgcgccacc
     6901 ttctctaggc accggttcaa ttgccgaccc ctccccccaa cttctcgggc actgtgggcc
     6961 atgtgcgctc tgccccatgg taatagccat gactaatacg tagatgtact gccaagtagg
     7021 aaagtgccat aaggtcatgt actgggcata atgccaggcg ggccatttac cgtcattgac
     7081 gtcaataggg ggcgtacttg gcatatgata cacttgatgt actgccaagt gggcagttta
     7141 ccgtaaatac tccacccatt gacgtcaatg gaaagtgcct attggcgtta ctatgggaac
     7201 atacgtcatt attgacgtca atgggcgggg gtcgttgggc ggtcagccag gcgggccatt
     7261 taccgtaagt tatgtaacgc ggaactccat atatgggcta tgaactaatg accccgtaat
     7321 tgattactat taataactag tcaataatca atgtcataag ctctaatcgc ataatcgagc
     7381 taagagcgat taatatgctt agctagctta gctaagctag atttctctat tctcccacaa
     7441 tatatagcag ctattagctt agcagtccga atacgatcgt ccacgatcca taagcgtaac
     7501 acatttgagt agcgactgac gggcaccgga gccactcgag tggaattatc acctcgagtt
     7561 tactccctat cagtgataga gaacgtatga agagtttact ccctatcagt gatagagaac
     7621 gtatgcagac tttactccct atcagtgata gagaacgtat aaggagttta ctccctatca
     7681 gtgatagaga acgtatgacc agtttactcc ctatcagtga tagagaacgt atctacagtt
     7741 tactccctat cagtgataga gaacgtatat ccagtttact ccctatcagt gatagagaac
     7801 gtat
//
