LOCUS       sequence-376996-       12683 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses FLAG tagged dCas12j3 VPR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12683)
  AUTHORS   Escobar M, Li J, Patel A, Liu S, Xu Q, Hilton IB
  TITLE     Quantification of Genome Editing and Transcriptional Control 
            Capabilities Reveals Hierarchies among Diverse CRISPR/Cas Systems in
            Human Cells.
  JOURNAL   ACS Synth Biol. 2022 Oct 21;11(10):3239-3250. doi: 
            10.1021/acssynbio.2c00156. Epub 2022 Sep 26.
  PUBMED    36162812
REFERENCE   2  (bases 1 to 12683)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.2c00156"; journalName: "ACS Synth Biol"; date: 
            "2022-10-21- 21"; volume: "11"; issue: "10"; pages: "3239-3250"
COMMENT     Sequence Label: dCas12j3 VPR
FEATURES             Location/Qualifiers
     source          1..12683
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..199
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     154..174
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             217..397
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    444..569
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1062..1295
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1480..1524
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1822..1939
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_RNA        2071..2146
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        2208..2419
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      2438..2447
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4742..4789
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             4790..4813
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    4856..4880
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4910..5059
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             5084..5104
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5534..5890
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALL"
     CDS             5909..6478
                     /codon_start=1
                     /product="transcriptional activation domain from the human 
                     herpesvirus 4 (Epstein-Barr virus) replication and 
                     transcription activator Rta/BRLF1 (Hardwick et al., 1992; 
                     Chavez et al., 2015)"
                     /label=Rta AD
                     /translation="RDSREGMFLPKPEAGSAISDVFEGREVCQPKRIRPFHPPGSPWAN
                     RPLPASLAPTPTGPVHEPVGSLTPAPVPQPLDPAPAVTPEASHLLEDPDEETSQAVKAL
                     REMADTVIPQKEEAAICGQMDLSHPPPRGHLDELTTTLESMTEDLNLDSPLTPELNEIL
                     DTFLNDECLLHAMHISTGLSIFDTSLF"
     CDS             6488..6544
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             6545..7141
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="TEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERV
                     TELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAA
                     QQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSA
                     PRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     7038..7058
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    7157..7745
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7210..7230)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7628..7639)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7817..8050
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(8076..8093)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8082..8306
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8352..8780
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8439..8458)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8649..8670
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8789..8809)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8794..9123
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8974..9109
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9036..9055
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        9171..9218
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9237..9611
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9741..9862
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9778..9797)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9832..9851
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9911..9927)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9911..9927)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9924..9946)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9935..9951
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9959..9965,9966..9983,9984..9989))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10004..10025
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10142..10159)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10313..10901)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10393..10412)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11072..11863,11864..11932))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11695..11714
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11933..12037)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(12112..12131)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        12303..12682
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc
       61 caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact
      121 ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt
      181 gggaggtcta tataagcagc gcgttttgcc tgtactgggt ctctctggtt agaccagatc
      241 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
      301 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
      361 ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga
      421 aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca
      481 cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct
      541 agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat
      601 gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat
      661 gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag
      721 gctgtagaca aatactggga cagctacaac catcccttca gacaggatca gaagaactta
      781 gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag
      841 acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac
      901 agcaagcggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt
      961 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca
     1021 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg
     1081 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc
     1141 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg
     1201 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg
     1261 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
     1321 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
     1381 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
     1441 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
     1501 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
     1561 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
     1621 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
     1681 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac
     1741 agagacagat ccattcgatt agtgaacgga tcggcactgc gtgcgccaat tctgcagaca
     1801 aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg
     1861 ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat
     1921 tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccag tttggttaat
     1981 taaggtaccg atatcactag tgttccatta actgcgctaa aaataatttt taaatctttt
     2041 ttagcttctt gctctttttt gtacgtctct gttttagagc tagaaatagc aagttaaaat
     2101 aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt ttgaattcgc
     2161 tagctaggtc ttgaaaggag tgggaattgg ctccggtgcc cgtcagtggg cagagcgcac
     2221 atcgcccaca gtccccgaga agttgggggg aggggtcggc aattgatccg gtgcctagag
     2281 aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc tttttcccga
     2341 gggtggggga gaaccgtata taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg
     2401 gtttgccgcc agaacacagg accggttcta gagcgctgcc accatggaaa aagaaataac
     2461 tgagctcacc aagattaggc gcgagtttcc gaataaaaag ttcagcagca ctgatatgaa
     2521 gaaggcaggt aagttgttga aggcagaagg tcctgatgct gttagagact tcctgaactc
     2581 ctgccaggag attatcgggg attttaagcc gcctgtaaag acaaacatag tcagcatatc
     2641 acgacccttt gaggagtggc ctgttagtat ggtggggcgc gccatccagg aatattactt
     2701 tagtttgaca aaagaggaat tggagtccgt ccatcccgga acttccagcg aggatcacaa
     2761 gtccttcttt aacataactg gcctgagcaa ttacaattat acgtcagtcc aaggcttgaa
     2821 tctcatcttc aaaaatgcga aggccatata cgacgggact ctggttaaag caaacaataa
     2881 aaataagaag ttggaaaaaa agttcaatga gattaaccac aagcgaagcc ttgaggggct
     2941 tcctataatt acgccggatt tcgaggaacc ctttgatgag aatggccatc tgaataatcc
     3001 gccaggtatt aatcgaaata tttacggcta ccaaggatgt gccgctaaag tattcgttcc
     3061 ttccaagcat aaaatggtat ccctccctaa agaatacgaa gggtacaacc gggatccgaa
     3121 cctgtccttg gcgggcttcc gaaatcggct cgagataccg gagggggagc ccggtcacgt
     3181 gccatggttt cagcgcatgg atatcccgga aggccagatc gggcacgtaa ataagattca
     3241 acgattcaat ttcgttcatg gcaagaattc aggaaaagtc aaattcagcg ataagacagg
     3301 acgggtaaaa cgctaccatc attccaagta taaagatgcc actaagcctt acaaatttct
     3361 tgaagaatcc aagaaagtca gtgctctgga ctccatcctt gccattatca caatcggtga
     3421 tgactgggta gtgtttgaca ttcgcggtct gtatagaaat gttttttatc gcgaactggc
     3481 acagaagggc ctgacagcag tgcagctgct ggatctgttt acgggggatc cggtgattga
     3541 cccgaagaag ggcgttgtga cattcagcta taaggaaggc gtggttccag tattttcaca
     3601 gaagatcgtt ccaaggttca agagtcgaga cacgctcgag aaattgacca gtcaaggacc
     3661 tgtggcgctg ctctcagtcg ccctcggcca aaatgaacca gtggcggcaa gggtttgtag
     3721 cttgaagaac ataaatgata agatcacatt ggataattct tgcagaatct ccttcctgga
     3781 tgactacaaa aaacaaatca aagactacag agattccctg gacgaacttg aaatcaagat
     3841 acgactggaa gcaatcaatt ctctggaaac taaccaacaa gtagaaattc gcgacctgga
     3901 tgtattcagt gctgatcggg caaaggcaaa cactgtagat atgttcgaca tcgacccaaa
     3961 tttgatatcc tgggattcaa tgagcgacgc gagggtgagc acgcaaataa gcgatcttta
     4021 tctgaagaat gggggtgacg aatctcgagt atatttcgaa attaacaaca aacggataaa
     4081 gcgatctgat tataacatta gtcagctggt gaggccaaag ctttccgaca gcactcggaa
     4141 gaatctgaac gattctatat ggaagttgaa aagaactagt gaagaatatt tgaaattgtc
     4201 caaacgaaag ttggaactga gcagagctgt tgtgaactac actatccgcc agagcaagct
     4261 cctctccgga attaacgaca ttgttataat acttgaggac ctggatgtaa aaaaaaaatt
     4321 caatggcagg ggcattcgag atatcggatg ggacaacttc ttcagctcca ggaaagagaa
     4381 caggtggttc attccggcat tccataaggc tttctcagag ctttcaagca accggggcct
     4441 ctgtgtcatc gaagtcaacc cggcatggac atctgccacc tgtcccgact gcgggttctg
     4501 tagtaaagag aacagagatg gcattaattt tacctgtcgc aagtgcggtg tctcttacca
     4561 cgcggacata gatgttgcca ctcttaatat agcccgggtg gccgttctcg gcaagcctat
     4621 gtccggaccc gccgaccgcg agagactggg cgatactaag aaaccccggg tagcaaggag
     4681 ccgaaagact atgaaacgga aagatattag caatagcacc gttgaggcta tggttacagc
     4741 caagcgacct gccgccacaa agaaggctgg acaggctaag aagaagaaag attacaaaga
     4801 cgatgacgat aagggatcct cgccagggat ccgtcgactt gacgcgttga tatcaacaag
     4861 tttgtacaaa aaagcaggct acaaagaggc cagcggttcc ggacgggctg acgcattgga
     4921 cgattttgat ctggatatgc tgggaagtga cgccctcgat gattttgacc ttgacatgct
     4981 tggttcggat gcccttgatg actttgacct cgacatgctc ggcagtgacg cccttgatga
     5041 tttcgacctg gacatgctga ttaactctag aagttccgga tctccgaaaa agaaacgcaa
     5101 agttggtagc cagtacctgc ccgacaccga cgaccggcac cggatcgagg aaaagcggaa
     5161 gcggacctac gagacattca agagcatcat gaagaagtcc cccttcagcg gccccaccga
     5221 ccctagacct ccacctagaa gaatcgccgt gcccagcaga tccagcgcca gcgtgccaaa
     5281 acctgccccc cagccttacc ccttcaccag cagcctgagc accatcaact acgacgagtt
     5341 ccctaccatg gtgttcccca gcggccagat ctctcaggcc tctgctctgg ctccagcccc
     5401 tcctcaggtg ctgcctcagg ctcctgctcc tgcaccagct ccagccatgg tgtctgcact
     5461 ggctcaggca ccagcacccg tgcctgtgct ggctcctgga cctccacagg ctgtggctcc
     5521 accagcccct aaacctacac aggccggcga gggcacactg tctgaagctc tgctgcagct
     5581 gcagttcgac gacgaggatc tgggagccct gctgggaaac agcaccgatc ctgccgtgtt
     5641 caccgacctg gccagcgtgg acaacagcga gttccagcag ctgctgaacc agggcatccc
     5701 tgtggcccct cacaccaccg agcccatgct gatggaatac cccgaggcca tcacccggct
     5761 cgtgacaggc gctcagaggc ctcctgatcc agctcctgcc cctctgggag caccaggcct
     5821 gcctaatgga ctgctgtctg gcgacgagga cttcagctct atcgccgata tggatttctc
     5881 agccttgctg ggctctggca gcggcagccg ggattccagg gaagggatgt ttttgccgaa
     5941 gcctgaggcc ggctccgcta ttagtgacgt gtttgagggc cgcgaggtgt gccagccaaa
     6001 acgaatccgg ccatttcatc ctccaggaag tccatgggcc aaccgcccac tccccgccag
     6061 cctcgcacca acaccaaccg gtccagtaca tgagccagtc gggtcactga ccccggcacc
     6121 agtccctcag ccactggatc cagcgcccgc agtgactccc gaggccagtc acctgttgga
     6181 ggatcccgat gaagagacga gccaggctgt caaagccctt cgggagatgg ccgatactgt
     6241 gattccccag aaggaagagg ctgcaatctg tggccaaatg gacctttccc atccgccccc
     6301 aaggggccat ctggatgagc tgacaaccac acttgagtcc atgaccgagg atctgaacct
     6361 ggactcaccc ctgaccccgg aattgaacga gattctggat accttcctga acgacgagtg
     6421 cctcttgcat gccatgcata tcagcacagg actgtccatc ttcgacacat ctctgtttgg
     6481 atccggcgca acaaacttct ctctgctgaa acaagccgga gatgtcgaag agaatcctgg
     6541 accgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt
     6601 acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga
     6661 ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga
     6721 catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga
     6781 gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg
     6841 ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga
     6901 gcccgcgtgg ttcctggcca ccgtcggagt ctcgcccgac caccagggca agggtctggg
     6961 cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct
     7021 ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc
     7081 cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg
     7141 aacgcgttaa gtcgacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat
     7201 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca
     7261 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc
     7321 tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc
     7381 tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt
     7441 cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg
     7501 gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc
     7561 ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta
     7621 cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg
     7681 gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc
     7741 cccgcgtcga ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta
     7801 aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga tctgcttttt
     7861 gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
     7921 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
     7981 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
     8041 atctctagca gggcccgttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca
     8101 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac
     8161 tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat
     8221 tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca
     8281 tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag
     8341 ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     8401 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
     8461 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
     8521 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
     8581 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     8641 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
     8701 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
     8761 acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc
     8821 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg
     8881 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     8941 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     9001 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     9061 tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
     9121 aaaaagctcc cgggagcttg tatatccatt ttcggatctg atcagcacgt gttgacaatt
     9181 aatcatcggc atagtatatc ggcatagtat aatacgacaa ggtgaggaac taaaccatgg
     9241 ccaagttgac cagtgccgtt ccggtgctca ccgcgcgcga cgtcgccgga gcggtcgagt
     9301 tctggaccga ccggctcggg ttctcccggg acttcgtgga ggacgacttc gccggtgtgg
     9361 tccgggacga cgtgaccctg ttcatcagcg cggtccagga ccaggtggtg ccggacaaca
     9421 ccctggcctg ggtgtgggtg cgcggcctgg acgagctgta cgccgagtgg tcggaggtcg
     9481 tgtccacgaa cttccgggac gcctccgggc cggccatgac cgagatcggc gagcagccgt
     9541 gggggcggga gttcgccctg cgcgacccgg ccggcaactg cgtgcacttc gtggccgagg
     9601 agcaggactg acacgtgcta cgagatttcg attccaccgc cgccttctat gaaaggttgg
     9661 gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc
     9721 tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca
     9781 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     9841 ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg
     9901 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
     9961 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
    10021 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
    10081 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
    10141 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
    10201 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
    10261 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
    10321 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
    10381 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
    10441 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
    10501 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
    10561 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
    10621 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
    10681 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
    10741 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
    10801 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
    10861 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
    10921 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
    10981 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
    11041 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
    11101 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
    11161 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
    11221 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
    11281 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
    11341 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
    11401 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
    11461 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
    11521 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
    11581 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
    11641 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
    11701 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
    11761 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
    11821 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
    11881 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
    11941 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
    12001 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
    12061 ctgacgtcga cggatcggga gatctcccga tcccctatgg tgcactctca gtacaatctg
    12121 ctctgatgcc gcatagttaa gccagtatct gctccctgct tgtgtgttgg aggtcgctga
    12181 gtagtgcgcg agcaaaattt aagctacaac aaggcaaggc ttgaccgaca attgcatgaa
    12241 gaatctgctt agggttaggc gttttgcgct gcttcgcgat gtacgggcca gatatacgcg
    12301 ttgacattga ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag
    12361 cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc
    12421 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg
    12481 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca
    12541 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc
    12601 ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt
    12661 attagtcatc gctattacca tgg
//
