LOCUS       sequence-376997-       13343 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses FLAG tagged DpbdCas12e VPR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13343)
  AUTHORS   Escobar M, Li J, Patel A, Liu S, Xu Q, Hilton IB
  TITLE     Quantification of Genome Editing and Transcriptional Control 
            Capabilities Reveals Hierarchies among Diverse CRISPR/Cas Systems in
            Human Cells.
  JOURNAL   ACS Synth Biol. 2022 Oct 21;11(10):3239-3250. doi: 
            10.1021/acssynbio.2c00156. Epub 2022 Sep 26.
  PUBMED    36162812
REFERENCE   2  (bases 1 to 13343)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.2c00156"; journalName: "ACS Synth Biol"; date: 
            "2022-10-21- 21"; volume: "11"; issue: "10"; pages: "3239-3250"
COMMENT     Sequence Label: DpbdCas12e VPR
FEATURES             Location/Qualifiers
     source          1..13343
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..199
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     154..174
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             217..397
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    444..569
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1062..1295
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1480..1524
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1822..1939
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_RNA        2071..2146
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        2208..2419
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      2438..2447
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5402..5449
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             5450..5473
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    5516..5540
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5570..5719
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             5744..5764
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6194..6550
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALL"
     CDS             6569..7138
                     /codon_start=1
                     /product="transcriptional activation domain from the human 
                     herpesvirus 4 (Epstein-Barr virus) replication and 
                     transcription activator Rta/BRLF1 (Hardwick et al., 1992; 
                     Chavez et al., 2015)"
                     /label=Rta AD
                     /translation="RDSREGMFLPKPEAGSAISDVFEGREVCQPKRIRPFHPPGSPWAN
                     RPLPASLAPTPTGPVHEPVGSLTPAPVPQPLDPAPAVTPEASHLLEDPDEETSQAVKAL
                     REMADTVIPQKEEAAICGQMDLSHPPPRGHLDELTTTLESMTEDLNLDSPLTPELNEIL
                     DTFLNDECLLHAMHISTGLSIFDTSLF"
     CDS             7148..7204
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             7205..7801
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="TEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERV
                     TELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAA
                     QQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSA
                     PRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     7698..7718
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    7817..8405
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7870..7890)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8288..8299)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8477..8710
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(8736..8753)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8742..8966
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      9012..9440
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9099..9118)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9309..9330
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9449..9469)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9454..9783
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9634..9769
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9696..9715
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        9831..9878
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9897..10271
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    10401..10522
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10438..10457)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10492..10511
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10571..10587)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10571..10587)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10584..10606)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10595..10611
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10619..10625,10626..10643,10644..10649))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10664..10685
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10802..10819)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10973..11561)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11053..11072)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11732..12523,12524..12592))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12355..12374
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12593..12697)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(12772..12791)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        12963..13342
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc
       61 caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact
      121 ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt
      181 gggaggtcta tataagcagc gcgttttgcc tgtactgggt ctctctggtt agaccagatc
      241 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
      301 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
      361 ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga
      421 aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca
      481 cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct
      541 agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat
      601 gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat
      661 gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag
      721 gctgtagaca aatactggga cagctacaac catcccttca gacaggatca gaagaactta
      781 gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag
      841 acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac
      901 agcaagcggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt
      961 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca
     1021 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg
     1081 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc
     1141 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg
     1201 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg
     1261 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
     1321 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
     1381 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
     1441 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
     1501 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
     1561 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
     1621 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
     1681 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac
     1741 agagacagat ccattcgatt agtgaacgga tcggcactgc gtgcgccaat tctgcagaca
     1801 aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg
     1861 ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat
     1921 tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccag tttggttaat
     1981 taaggtaccg atatcactag tgttccatta actgcgctaa aaataatttt taaatctttt
     2041 ttagcttctt gctctttttt gtacgtctct gttttagagc tagaaatagc aagttaaaat
     2101 aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt ttgaattcgc
     2161 tagctaggtc ttgaaaggag tgggaattgg ctccggtgcc cgtcagtggg cagagcgcac
     2221 atcgcccaca gtccccgaga agttgggggg aggggtcggc aattgatccg gtgcctagag
     2281 aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc tttttcccga
     2341 gggtggggga gaaccgtata taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg
     2401 gtttgccgcc agaacacagg accggttcta gagcgctgcc accatggaaa agagaatcaa
     2461 caagatcagg aagaagctga gcgccgacaa cgccaccaag cctgtgtcta ggagtggccc
     2521 catgaaaacc ctgctcgtca gagtgatgac cgacgacctg aagaagagac tggaaaagag
     2581 gaggaagaaa cccgaagtga tgccccaagt gatcagcaac aacgccgcca acaacctgag
     2641 aatgctgctg gacgactaca ccaagatgaa ggaagccatc ctgcaggtct actggcaaga
     2701 gttcaaggac gaccacgtgg gcctgatgtg caagtttgcc cagcctgcca gcaagaagat
     2761 cgaccagaac aagctgaagc ccgagatgga cgagaagggc aatctgacca cagccggctt
     2821 tgcctgctct cagtgtggcc agcctctgtt cgtgtacaag ctggaacagg tgtccgagaa
     2881 aggcaaggcc tacaccaact acttcggcag atgtaacgtg gccgagcacg agaagctgat
     2941 tctgctggcc cagctgaaac ctgagaagga ctctgatgag gccgtgacct acagcctggg
     3001 caagtttgga cagagagccc tggacttcta cagcatccac gtgaccaaag aaagcacaca
     3061 ccccgtgaag cccctggctc agatcgccgg caatagatac gcctctggac ctgtgggcaa
     3121 agccctgtcc gatgcctgca tgggaacaat cgccagcttc ctgagcaagt accaggacat
     3181 catcatcgag caccagaagg tggtcaaggg caaccagaag agactggaaa gcctgaggga
     3241 gctggccggc aaagagaacc tggaataccc cagcgtgacc ctgcctcctc agcctcacac
     3301 aaaagaaggc gtggacgcct acaacgaagt gatcgccaga gtgagaatgt gggtcaacct
     3361 gaacctgtgg cagaagctga aactgtccag ggacgacgcc aagcctctgc tgagactgaa
     3421 gggcttccct agcttccccg tggtggaaag aagggagaat gaggtggact ggtggaacac
     3481 catcaacgaa gtcaagaaac tgatcgatgc caagagagac atgggcagag tgttttggag
     3541 cggagtgacc gccgagaaga gaaacaccat cctggaaggc tacaactacc tgcctaacga
     3601 gaacgaccac aagaagagag agggcagcct ggaaaaccct aagaagcccg ccaagagaca
     3661 gttcggcgac ctgctgctgt acctggagaa gaagtatgcc ggcgactggg gcaaagtgtt
     3721 cgatgaggcc tgggagagaa tcgacaagaa gattgccggc ctgaccagcc acatcgagag
     3781 agaggaagcc agaaacgccg aggacgccca gtctaaagcc gtgctgacag attggctgag
     3841 agccaaggcc agcttcgtgc tggaaaggct gaaagaaatg gatgagaaag agttctacgc
     3901 ctgcgagatc cagctgcaga agtggtacgg ggacctgaga ggcaatccct ttgccgtgga
     3961 agccgagaac agagtggtgg acatcagcgg ctttagcatc ggctctgacg gccactccat
     4021 ccagtacagg aacctgctgg cctggaagta cctggaaaat ggcaagagag agttctacct
     4081 gctgatgaac tacggcaaga agggcagaat cagattcacc gacggcaccg acatcaagaa
     4141 aagcggcaag tggcagggcc tgctgtatgg cggcggaaag gccaaagtga tcgacctgac
     4201 cttcgatccc gacgacgagc agctgatcat tctgcctctg gccttcggca ccagacaggg
     4261 cagagagttc atctggaacg atctgctgtc cctggaaacc ggcctgatca agctggccaa
     4321 cggcagggtg atcgagaaaa ccatctacaa caagaaaatc ggcagagatg agcccgcact
     4381 gtttgtggcc ctgacctttg agaggagaga agtggtcgat cccagcaaca tcaagcccgt
     4441 gaacctgatc ggagtggcca gaggcgagaa tatccctgcc gtgattgccc tgacagaccc
     4501 tgagggatgt cccctgcctg agtttaagga tagctctggc ggccctaccg acatcctgag
     4561 aatcggcgag ggctacaaag agaagcagag agccatccag gccgccaaag aggtggaaca
     4621 gagaagagcc ggcggataca gcagaaagtt cgccagcaag tccagaaacc tggccgatga
     4681 catggtcagg aacagcgcca gggacctgtt ctaccacgcc gtgacacatg atgccgtgct
     4741 ggtgttcgcc aacctgtcaa gaggcttcgg cagacaggga aaaagaacct tcatgaccga
     4801 gagacagtac acgaagatgg aagattggct caccgccaaa ctggcctacg agggcctgac
     4861 aagcaagacc tacctgtcca agacactggc ccagtacacc tccaagacct gctccaattg
     4921 cggcttcacc atcaccaccg ccgactacga tggaatgctc gtgagactga agaaaacctc
     4981 cgacggctgg gccaccacac tgaacaacaa agagctgaaa gccgagggcc agatcaccta
     5041 ctacaacaga tacaagaggc agaccgtcga gaaagagctg tccgccgaac tcgacagact
     5101 gagcgaggaa tccggcaaca acgacatcag caagtggacc aagggacgca gagatgaggc
     5161 cctgttcctg ctgaagaaga ggttcagcca cagacctgtg caagagcagt ttgtgtgcct
     5221 ggattgcggc cacgaagtgc acgcagccga acaggccgct ctgaatatcg ccagaagctg
     5281 gctgttcctg aacagcaaca gcaccgagtt caagagctac aagagcggca agcagccttt
     5341 cgttggagcc tggcaggcct tctacaagag aaggctcaaa gaagtgtgga agcccaacgc
     5401 caagcgacct gccgccacaa agaaggctgg acaggctaag aagaagaaag attacaaaga
     5461 cgatgacgat aagggatcct cgccagggat ccgtcgactt gacgcgttga tatcaacaag
     5521 tttgtacaaa aaagcaggct acaaagaggc cagcggttcc ggacgggctg acgcattgga
     5581 cgattttgat ctggatatgc tgggaagtga cgccctcgat gattttgacc ttgacatgct
     5641 tggttcggat gcccttgatg actttgacct cgacatgctc ggcagtgacg cccttgatga
     5701 tttcgacctg gacatgctga ttaactctag aagttccgga tctccgaaaa agaaacgcaa
     5761 agttggtagc cagtacctgc ccgacaccga cgaccggcac cggatcgagg aaaagcggaa
     5821 gcggacctac gagacattca agagcatcat gaagaagtcc cccttcagcg gccccaccga
     5881 ccctagacct ccacctagaa gaatcgccgt gcccagcaga tccagcgcca gcgtgccaaa
     5941 acctgccccc cagccttacc ccttcaccag cagcctgagc accatcaact acgacgagtt
     6001 ccctaccatg gtgttcccca gcggccagat ctctcaggcc tctgctctgg ctccagcccc
     6061 tcctcaggtg ctgcctcagg ctcctgctcc tgcaccagct ccagccatgg tgtctgcact
     6121 ggctcaggca ccagcacccg tgcctgtgct ggctcctgga cctccacagg ctgtggctcc
     6181 accagcccct aaacctacac aggccggcga gggcacactg tctgaagctc tgctgcagct
     6241 gcagttcgac gacgaggatc tgggagccct gctgggaaac agcaccgatc ctgccgtgtt
     6301 caccgacctg gccagcgtgg acaacagcga gttccagcag ctgctgaacc agggcatccc
     6361 tgtggcccct cacaccaccg agcccatgct gatggaatac cccgaggcca tcacccggct
     6421 cgtgacaggc gctcagaggc ctcctgatcc agctcctgcc cctctgggag caccaggcct
     6481 gcctaatgga ctgctgtctg gcgacgagga cttcagctct atcgccgata tggatttctc
     6541 agccttgctg ggctctggca gcggcagccg ggattccagg gaagggatgt ttttgccgaa
     6601 gcctgaggcc ggctccgcta ttagtgacgt gtttgagggc cgcgaggtgt gccagccaaa
     6661 acgaatccgg ccatttcatc ctccaggaag tccatgggcc aaccgcccac tccccgccag
     6721 cctcgcacca acaccaaccg gtccagtaca tgagccagtc gggtcactga ccccggcacc
     6781 agtccctcag ccactggatc cagcgcccgc agtgactccc gaggccagtc acctgttgga
     6841 ggatcccgat gaagagacga gccaggctgt caaagccctt cgggagatgg ccgatactgt
     6901 gattccccag aaggaagagg ctgcaatctg tggccaaatg gacctttccc atccgccccc
     6961 aaggggccat ctggatgagc tgacaaccac acttgagtcc atgaccgagg atctgaacct
     7021 ggactcaccc ctgaccccgg aattgaacga gattctggat accttcctga acgacgagtg
     7081 cctcttgcat gccatgcata tcagcacagg actgtccatc ttcgacacat ctctgtttgg
     7141 atccggcgca acaaacttct ctctgctgaa acaagccgga gatgtcgaag agaatcctgg
     7201 accgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt
     7261 acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga
     7321 ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga
     7381 catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga
     7441 gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg
     7501 ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga
     7561 gcccgcgtgg ttcctggcca ccgtcggagt ctcgcccgac caccagggca agggtctggg
     7621 cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct
     7681 ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc
     7741 cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg
     7801 aacgcgttaa gtcgacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat
     7861 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca
     7921 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc
     7981 tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc
     8041 tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt
     8101 cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg
     8161 gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc
     8221 ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta
     8281 cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg
     8341 gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc
     8401 cccgcgtcga ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta
     8461 aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga tctgcttttt
     8521 gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
     8581 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
     8641 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
     8701 atctctagca gggcccgttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca
     8761 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac
     8821 tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat
     8881 tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca
     8941 tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag
     9001 ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     9061 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
     9121 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
     9181 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
     9241 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     9301 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
     9361 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
     9421 acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc
     9481 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg
     9541 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     9601 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     9661 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     9721 tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
     9781 aaaaagctcc cgggagcttg tatatccatt ttcggatctg atcagcacgt gttgacaatt
     9841 aatcatcggc atagtatatc ggcatagtat aatacgacaa ggtgaggaac taaaccatgg
     9901 ccaagttgac cagtgccgtt ccggtgctca ccgcgcgcga cgtcgccgga gcggtcgagt
     9961 tctggaccga ccggctcggg ttctcccggg acttcgtgga ggacgacttc gccggtgtgg
    10021 tccgggacga cgtgaccctg ttcatcagcg cggtccagga ccaggtggtg ccggacaaca
    10081 ccctggcctg ggtgtgggtg cgcggcctgg acgagctgta cgccgagtgg tcggaggtcg
    10141 tgtccacgaa cttccgggac gcctccgggc cggccatgac cgagatcggc gagcagccgt
    10201 gggggcggga gttcgccctg cgcgacccgg ccggcaactg cgtgcacttc gtggccgagg
    10261 agcaggactg acacgtgcta cgagatttcg attccaccgc cgccttctat gaaaggttgg
    10321 gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc
    10381 tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca
    10441 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
    10501 ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg
    10561 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
    10621 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
    10681 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
    10741 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
    10801 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
    10861 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
    10921 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
    10981 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
    11041 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
    11101 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
    11161 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
    11221 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
    11281 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
    11341 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
    11401 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
    11461 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
    11521 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
    11581 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
    11641 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
    11701 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
    11761 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
    11821 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
    11881 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
    11941 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
    12001 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
    12061 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
    12121 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
    12181 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
    12241 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
    12301 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
    12361 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
    12421 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
    12481 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
    12541 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
    12601 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
    12661 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
    12721 ctgacgtcga cggatcggga gatctcccga tcccctatgg tgcactctca gtacaatctg
    12781 ctctgatgcc gcatagttaa gccagtatct gctccctgct tgtgtgttgg aggtcgctga
    12841 gtagtgcgcg agcaaaattt aagctacaac aaggcaaggc ttgaccgaca attgcatgaa
    12901 gaatctgctt agggttaggc gttttgcgct gcttcgcgat gtacgggcca gatatacgcg
    12961 ttgacattga ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag
    13021 cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc
    13081 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg
    13141 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca
    13201 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc
    13261 ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt
    13321 attagtcatc gctattacca tgg
//
