LOCUS       sequence-376999-       12335 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses C-terminal flag-tagged human CAD N1394A in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12335)
  AUTHORS   Shin J, Mir H, Khurram MA, Fujihara KM, Dynlacht BD, Cardozo TJ, 
            Possemato R
  TITLE     Allosteric regulation of CAD modulates de novo pyrimidine synthesis 
            during the cell cycle.
  JOURNAL   Nat Metab. 2023 Feb 6. doi: 10.1038/s42255-023-00735-9.
  PUBMED    36747088
REFERENCE   2  (bases 1 to 12335)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Metab. 
            2023 Feb 6. doi: 10.1038/s42255-023-00735-9."
COMMENT     Sequence Label: pMXS-IRES-BLAST flag-CAD N1394A
FEATURES             Location/Qualifiers
     source          1..12335
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(152..169)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     379..398
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             572..967
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MVPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTGV
                     NVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGIK
                     AIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             1138..1607
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(1737..1754)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1908..2496)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1988..2007)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2660..3451,3452..3520))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3283..3302
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3521..3625)
                     /gene="bla"
                     /label=AmpR promoter
     LTR             3655..4248
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    4311..4668
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             4727..5143
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     5073..5095
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     5111..5127
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    5153..5527
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     5465..5481
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     regulatory      5554..5563
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             12231..12254
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    join(12320..12335,1..559,560..562,563..571)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
ORIGIN
        1 ccccctaacg ttactggccg aagccgcttg gaataaggcc ggtgtgcgtt tgtctatatg
       61 ttattttcca ccatattgcc gtcttttggc aatgtgaggg cccggaaacc tggccctgtc
      121 ttcttgacga gcattcctag gggtctttcc cctctcgcca aaggaatgca aggtctgttg
      181 aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa gacaaacaac gtctgtagcg
      241 accctttgca ggcagcggaa ccccccacct ggcgacaggt gcctctgcgg ccaaaagcca
      301 cgtgtataag atacacctgc aaaggcggca caaccccagt gccacgttgt gagttggata
      361 gttgtggaaa gagtcaaatg gctctcctca agcgtattca acaaggggct gaaggatgcc
      421 cagaaggtac cccattgtat gggatctgat ctggggcctc ggtgcacatg ctttacatgt
      481 gtttagtcga ggttaaaaaa acgtctaggc cccccgaacc acggggacgt ggttttcctt
      541 tgaaaaacac gatgataata tggccacaac catggttcct ttgtctcaag aagaatccac
      601 cctcattgaa agagcaacgg ctacaatcaa cagcatcccc atctctgaag actacagcgt
      661 cgccagcgca gctctctcta gcgacggccg catcttcact ggtgtcaatg tatatcattt
      721 tactggggga ccttgtgcag aactcgtggt gctgggcact gctgctgctg cggcagctgg
      781 caacctgact tgtatcgtcg cgatcggaaa tgagaacagg ggcatcttga gcccctgcgg
      841 acggtgccga caggtgcttc tcgatctgca tcctgggatc aaagccatag tgaaggacag
      901 tgatggacag ccgacggcag ttgggattcg tgaattgctg ccctctggtt atgtgtggga
      961 gggctaagtc gacgataaaa taaaagattt tatttagtct ccagaaaaag gggggaatga
     1021 aagaccccac ctgtaggttt ggcaagctag cttaagtaac gccattttgc aaggcatgga
     1081 aaaatacata actgagaata gagaagttca gatcaaggtc aggaacagat ggaacagctg
     1141 aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca gggccaagaa
     1201 cagatggaac agctgaatat gggccaaaca ggatatctgt ggtaagcagt tcctgccccg
     1261 gctcagggcc aagaacagat ggtccccaga tgcggtccag ccctcagcag tttctagaga
     1321 accatcagat gtttccaggg tgccccaagg acctgaaatg accctgtgcc ttatttgaac
     1381 taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc tgctccccga gctcaataaa
     1441 agagcccaca acccctcact cggggcgcca gtcctccgat tgactgagtc gcccgggtac
     1501 ccgtgtatcc aataaaccct cttgcagttg catccgactt gtggtctcgc tgttccttgg
     1561 gagggtctcc tctgagtgat tgactacccg tcagcggggg tctttcacat gcagcatgta
     1621 tcaaaattaa tttggttttt tttcttaagt atttacatta aatggccata gttgcattaa
     1681 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     1741 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     1801 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     1861 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     1921 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     1981 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     2041 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     2101 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     2161 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     2221 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     2281 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     2341 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     2401 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     2461 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     2521 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     2581 aaaaggatct tcacctagat ccttttgcgg ccggccgcaa atcaatctaa agtatatatg
     2641 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     2701 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     2761 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     2821 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     2881 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     2941 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     3001 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     3061 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     3121 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     3181 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     3241 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     3301 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     3361 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     3421 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     3481 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     3541 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     3601 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gcataatgaa
     3661 agaccccacc tgtaggtttg gcaagctagc ttaagtaacg ccattttgca aggcatggaa
     3721 aaatacataa ctgagaatag aaaagttcag atcaaggtca ggaacagatg gaacagctga
     3781 atatgggcca aacaggatat ctgtggtaag cagttcctgc cccggctcag ggccaagaac
     3841 agatggaaca gctgaatatg ggccaaacag gatatctgtg gtaagcagtt cctgccccgg
     3901 ctcagggcca agaacagatg gtccccagat gcggtccagc cctcagcagt ttctagagaa
     3961 ccatcagatg tttccagggt gccccaagga cctgaaatga ccctgtgcct tatttgaact
     4021 aaccaatcag ttcgcttctc gcttctgttc gcgcgcttct gctccccgag ctcaataaaa
     4081 gagcccacaa cccctcactc ggcgcgccag tcctccgatt gactgagtcg cccgggtacc
     4141 cgtgtatcca ataaaccctc ttgcagttgc atccgacttg tggtctcgct gttccttggg
     4201 agggtctcct ctgagtgatt gactacccgt cagcgggggt ctttcatttg ggggctcgtc
     4261 cgggatcggg agacccctgc ccagggacca ccgacccacc accgggaggt aagctggcca
     4321 gcaacttatc tgtgtctgtc cgattgtcta gtgtctatga ctgattttat gcgcctgcgt
     4381 cggtactagt tagctaacta gctctgtatc tggcggaccc gtggtggaac tgacgagttc
     4441 ggaacacccg gccgcaaccc tgggagacgt cccagggact tcgggggccg tttttgtggc
     4501 ccgacctgag tccaaaaatc ccgatcgttt tggactcttt ggtgcacccc cctaatagga
     4561 gggatatgtg gttctggtag gagacgagaa cctaaaacag ttcccgcctc cgtctgaatt
     4621 tttgctttcg gtttgggacc gaagccgcgc cgcgcgtctt gtctgctgca gcatcgttct
     4681 gtgttgtctc tgtctgactg tgtttctgta tttgtctgaa aattagggcc agactgttac
     4741 cactccctta agtttgacct taggtcactg gaaagatgtc gagcggatcg ctcacaacca
     4801 gtcggtagat gtcaagaaga gacgttgggt taccttctgc tctgcagaat ggccaacctt
     4861 taacgtcgga tggccgcgag acggcacctt taaccgagac ctcatcaccc aggttaagat
     4921 caaggtcttt tcacctggcc cgcatggaca cccagaccag gtcccctaca tcgtgacctg
     4981 ggaagccttg gcttttgacc cccctccctg ggtcaagccc tttgtacacc ctaagcctcc
     5041 gcctcctctt cctccatccg ccccgtctct cccccttgaa cctcctcgtt cgaccccgcc
     5101 tcgatcctcc ctttatccag ccctcactcc ttctctaggc gcccccatat ggccatatga
     5161 gatcttatat ggggcacccc cgccccttgt aaacttccct gaccctgaca tgacaagagt
     5221 tactaacagc ccctctctcc aagctcactt acaggctctc tacttagtcc agcacgaagt
     5281 ctggagacct ctggcggcag cctaccaaga acaactggac cgaccggtgg tacctcaccc
     5341 ttaccgagtc ggcgacacag tgtgggtccg ccgacaccag actaagaacc tagaacctcg
     5401 ctggaaagga ccttacacag tcctgctgac cacccccacc gccctcaaag tagacggcat
     5461 cgcagcttgg atacacgccg cccacgtgaa ggctgccgac cccgggggtg gaccatcctc
     5521 tagactgccg gatctagcta gttaattaag gatccatggc ggccctagtg ttggaggacg
     5581 ggtcggtcct gcggggccag ccctttgggg ccgccgtgtc gactgccggg gaagtggtgt
     5641 ttcaaaccgg catggtcggc taccccgagg ccctcactga tccctcctac aaggcacaga
     5701 tcttagtgct cacctatcct ctgatcggca actatggcat ccccccagat gaaatggatg
     5761 agttcggtct ctgcaagtgg tttgaatcct cgggcatcca cgtagcagca ctggtagtgg
     5821 gagagtgctg tcctactccc agccactgga gtgccacccg caccctgcat gagtggctgc
     5881 agcagcatgg catccctggc ttgcaaggag tagacactcg ggagctgacc aagaagttgc
     5941 gggaacaggg gtctctgctg gggaagctgg tccagaatgg aacagaacct tcaagtctgc
     6001 cattcttgga ccccaatgcc cgccccctgg taccagaggt ctccattaag actccacggg
     6061 tattcaatac agggggtgcc cctcggatcc ttgctttgga ctgtggcctc aagtataatc
     6121 agatccgatg cctctgccag cgtggggctg aggtcactgt ggtaccctgg gaccatgcac
     6181 tagacagcca agagtatgag ggtctcttct taagtaatgg gcctggtgac cctgcctcct
     6241 atcccagtgt cgtatccaca ctgagccgtg ttttatctga gcctaatccc cgacctgtct
     6301 ttgggatctg cctgggacac cagctattgg ccttagccat tggggccaag acttacaaga
     6361 tgagatatgg gaaccgaggc cataaccagc cctgcttgtt ggtgggctct gggcgctgct
     6421 ttctgacatc ccagaaccat gggtttgctg tggagacaga ctcactgcca gcagactggg
     6481 ctcctctctt caccaacgcc aatgatggtt ccaatgaagg cattgtgcac aacagcttgc
     6541 ctttcttcag tgtccagttt cacccagagc accaagctgg cccttcagat atggaactgc
     6601 ttttcgatat ctttctggaa actgtgaaag aggccacagc tgggaaccct gggggccaga
     6661 cagttagaga gcggctgact gagcgcctct gtccccctgg gattcccact cccggctctg
     6721 gacttccacc accacgaaag gttctgatcc tgggctcagg gggcctctcc attggccaag
     6781 ctggagaatt tgactactcg ggctctcagg caattaaggc cctgaaggag gaaaacatcc
     6841 agacgttgct gatcaacccc aatattgcca cagtgcagac ctcccagggg ctggccgaca
     6901 aggtctattt tcttcccata acacctcatt atgtaaccca ggtgatacgt aatgaacgcc
     6961 ccgatggtgt gttactgact tttgggggcc agactgctct gaactgtggt gtggagctga
     7021 ccaaggccgg ggtgctggct cggtatgggg tccgggtcct gggcacacca gtggagacca
     7081 ttgagctgac cgaggatcga cgggcctttg ctgccagaat ggcagagatc ggagagcatg
     7141 tggccccgag cgaggcagca aattctcttg aacaggccca ggcagccgct gaacggctgg
     7201 ggtaccctgt gctagtgcgt gcagcctttg ccctgggtgg cctgggctct ggctttgcct
     7261 ctaacaggga ggagctctct gctctcgtgg ccccagcttt tgcccatacc agccaagtgc
     7321 tagtagacaa gtctctgaag ggatggaagg agattgagta cgaggtggtg agagacgcct
     7381 atggcaactg tgtcacggtg tgtaacatgg agaacttgga cccactgggc atccacactg
     7441 gtgagtccat agtggtggcc cctagccaga cactgaatga cagggagtat cagctcctga
     7501 ggcagacagc tatcaaggtg acccagcacc tgggaattgt tggggagtgc aatgtgcagt
     7561 atgccttgaa ccctgagtct gagcagtatt acatcattga agtgaatgcc aggctctctc
     7621 gcagctctgc cctggccagt aaggccacag gttatccact ggcttatgtg gcagccaagc
     7681 tagcattggg catccctttg cctgagctca ggaactctgt gacagggggt acagcagcct
     7741 ttgaacccag cgtggattat tgtgtggtga agattcctcg atgggacctt agcaagttcc
     7801 tgcgagtcag cacaaagatt gggagctgca tgaagagcgt tggtgaagtc atgggcattg
     7861 ggcgttcatt tgaggaggcc ttccagaagg ccctgcgcat ggtggatgag aactgtgtgg
     7921 gctttgatca cacagtgaaa ccagtcagcg atatggagtt ggagactcca acagataagc
     7981 ggatttttgt ggtggcagct gctttgtggg ctggttattc agtggaccgc ctgtatgagc
     8041 tcacacgcat cgaccgctgg ttcctgcacc gaatgaagcg tatcatcgca catgcccagc
     8101 tgctagaaca acaccgtgga cagcctttgc cgccagacct gctgcaacag gccaagtgtc
     8161 ttggcttctc agacaaacag attgcccttg cagttctgag cacagagctg gctgttcgca
     8221 agctgcgtca ggaactgggg atctgtccag cagtgaaaca gattgacaca gttgcagctg
     8281 agtggccagc ccagacaaat tacctatacc taacgtattg gggcaccacc catgacctca
     8341 cctttcgaac acctcatgtc ctagtccttg gctctggcgt ctaccgtatt ggctctagcg
     8401 ttgaatttga ctggtgtgct gtaggctgca tccagcagct ccgaaagatg ggatataaga
     8461 ccatcatggt gaactataac ccagagacag tcagcaccga ctatgacatg tgtgatcgac
     8521 tctactttga tgagatctct tttgaggtgg tgatggacat ctatgagctc gagaaccctg
     8581 aaggtgtgat cctatccatg ggtggacagc tgcccaacaa catggccatg gcgttgcatc
     8641 ggcagcagtg ccgggtgctg ggcacctccc ctgaagccat tgactcggct gagaaccgtt
     8701 tcaagttttc ccggctcctt gacaccattg gtatcagcca gcctcagtgg agggagctca
     8761 gtgacctcga gtctgctcgc caattctgcc agaccgtggg gtacccctgt gtggtgcgcc
     8821 cctcctatgt gctgagcggt gctgctatga atgtggccta cacggatgga gacctggagc
     8881 gcttcctgag cagcgcagca gccgtctcca aagagcatcc cgtggtcatc tccaagttca
     8941 tccaggaggc taaggagatt gacgtggatg ccgtggcctc tgatggtgtg gtggcagcca
     9001 tcgccatctc tgagcatgtg gagaatgcag gtgtgcattc aggtgatgcg acgctggtga
     9061 cccccccaca agatatcact gccaaaaccc tggagcggat caaagccatt gtgcatgctg
     9121 tgggccagga gctacaggtc acaggaccct tcaatctgca gctcattgcc aaggatgacc
     9181 agctgaaagt tattgaatgc aacgtacgtg tctctcgctc cttccccttc gtttccaaga
     9241 cactgggtgt ggacctagta gccttggcca cgcgggtcat catgggggaa gaagtggaac
     9301 ctgtggggct aatgactggt tctggagttg tgggagtaaa ggtgcctcag ttctccttct
     9361 cccgcttggc gggtgctgac gtggtgttgg gtgtggaaat gaccagtact ggggaggtgg
     9421 ccggctttgg ggagagccgc tgtgaggcat acctcaaggc catgctaagc actggcttta
     9481 agatccccaa gaagaatatc ctgctgacca ttggcagcta taagaacaaa agcgagctgc
     9541 tcccaactgt gcggctactg gagagcctgg gctacagcct ctatgccagt ctcggcacag
     9601 ctgacttcta cactgagcat ggcgtcaagg taacagctgt ggactggcac tttgaggagg
     9661 ctgtggatgg tgagtgccca ccacagcgga gcatcctgga gcagctagct gagaaaaact
     9721 ttgagctggt gattgccctg tcaatgcgtg gagctggggg ccggcgtctc tcttcctttg
     9781 tcaccaaggg ctaccgcacc cgacgcttgg ccgctgactt ctccgtgccc ctaatcatcg
     9841 atatcaagtg caccaaactc tttgtggagg ccctaggcca gatcgggcca gcccctcctt
     9901 tgaaggtgca tgttgactgt atgacctccc aaaagcttgt gcgactgccg ggattgattg
     9961 atgtccatgt gcacctgcgg gaaccaggtg ggacacataa ggaggacttt gcttcaggca
    10021 cagccgctgc cctggctggg ggtatcacca tggtgtgtgc catgcctaat acccggcccc
    10081 ccatcattga cgcccctgct ctggccctgg cccagaagct ggcagaggct ggcgcccggt
    10141 gcgactttgc gctattcctt ggggcctcgt ctgaaaatgc aggaaccttg ggcaccgtgg
    10201 ccgggtctgc agccgggctg aagctttacc tcaatgagac cttctctgag ctgcggctgg
    10261 acagcgtggt ccagtggatg gagcatttcg agacatggcc ctcccacctc cccattgtgg
    10321 ctcacgcgga gcagcaaacc gtggctgctg tcctcatggt ggctcagctc actcagcgct
    10381 cagtgcacat atgtcacgtg gcacggaagg aggagatcct gctaattaaa gctgcaaagg
    10441 cacggggctt gccagtgacc tgcgaggtgg ctccccacca cctgttccta agccatgatg
    10501 acctggagcg cctggggcct gggaaggggg aggtccggcc tgagcttggc tcccgccagg
    10561 atgtggaagc cctgtgggag aacatggctg tcatcgactg ctttgcctca gaccatgctc
    10621 cccatacctt ggaggagaag tgtgggtcca ggcccccacc tgggttccca gggttagaga
    10681 ccatgctgcc actactcctg acggctgtaa gcgagggccg gctcagcctg gacgacctgc
    10741 tgcagcgatt gcaccacaat cctcggcgca tctttcacct gcctccgcag gaggacacct
    10801 atgtggaggt ggatctggag catgagtgga caattcccag ccacatgccc ttctccaagg
    10861 cccactggac accttttgaa gggcagaaag tgaagggcac cgtccgccgt gtggtcctgc
    10921 gaggggaggt tgcctatatc gatgggcagg ttctggtacc cccgggctat ggacaggatg
    10981 tacggaagtg gccacagggg gctgttcctc agctcccacc ctcagcccct gccactagtg
    11041 agatgaccac gacacctgaa agaccccgcc gtggcatccc agggcttcct gatggccgct
    11101 tccatctgcc gccccgaatc catcgagcct ccgacccagg tttgccagct gaggagccaa
    11161 aggagaagtc ctctcggaag gtagccgagc cagagctgat gggaacccct gatggcacct
    11221 gctaccctcc accaccagta ccgagacagg catctcccca gaacctgggg acccctggct
    11281 tgctgcaccc ccagacctca cccctgctgc actcattagt gggccaacat atcctgtccg
    11341 tccagcagtt caccaaggat cagatgtctc acctgttcaa tgtggcacac acactgcgta
    11401 tgatggtgca gaaggagcgg agcctcgaca tcctgaaggg gaaggtcatg gcctccatgt
    11461 tctatgaagt gagcacacgg accagcagct cctttgcagc agccatggcc cggctgggag
    11521 gtgctgtgct cagcttctcg gaagccacat cgtccgtcca gaagggcgaa tccctggctg
    11581 actccgtgca gaccatgagc tgctatgccg acgtcgtcgt gctccggcac ccccagcctg
    11641 gagcagtgga gctggccgcc aagcactgcc ggaggccagt gatcaatgct ggggatgggg
    11701 tcggagagca ccccacccag gccctgctgg acatcttcac catccgtgag gagctgggaa
    11761 ctgtcaatgg catgacgatc acgatggtgg gtgacctgaa gcacggacgc acagtacatt
    11821 ccctggcctg cctgctcacc cagtatcgtg tcagcctgcg ctacgtggca cctcccagcc
    11881 tgcgcatgcc acccactgtg cgggccttcg tggcctcccg cggcaccaag caggaggaat
    11941 tcgagagcat tgaggaggcg ctgcctgaca ctgatgtgct ctacatgact cgaatccaga
    12001 aggaacgatt tggctctacc caggagtacg aagcttgctt tggtcagttc atcctcactc
    12061 cccacatcat gacccgggcc aagaagaaga tggtggtgat gcacccgatg ccccgtgtca
    12121 acgagataag cgtggaagtg gactcggatc cccgcgcagc ctacttccgc caggctgaga
    12181 acggcatgta catccgcatg gctctgttag ccaccgtgct gggccgtttc gactacaaag
    12241 acgatgacga caagtaggaa ttcctgcagg cctcgagggc cggcgcgccg cggccgctac
    12301 gtaaattccg cccccccccc ccctctccct ccccc
//
