LOCUS       sequence-377013-        2354 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  L0 MoClo-compatible GoldenGate-module of the NOS terminator (tNOS) 
            with stop.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2354)
  AUTHORS   Schindele P, Merker L, Schreiber T, Prange A, Tissier A, Puchta H
  TITLE     Enhancing gene editing and gene targeting efficiencies in 
            Arabidopsis thaliana by using an intron-containing version of 
            ttLbCas12a.
  JOURNAL   Plant Biotechnol J. 2023 Mar;21(3):457-459. doi: 10.1111/pbi.13964. 
            Epub 2022 Nov 30.
  PUBMED    36382936
REFERENCE   2  (bases 1 to 2354)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/pbi.13964";
            journalName: "Plant Biotechnol J"; date: "2023-03"; volume: "21"; 
            issue: "3"; pages: "457-459"
COMMENT     Sequence Label: pAGT9403
FEATURES             Location/Qualifiers
     source          1..2354
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             297..1088
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1429..2017
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1918..1937
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      2096..2348
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
ORIGIN
        1 cgagaccacg aagtgccgag gcacgaaccc agtggacata agcctgttcg gttcgtaagc
       61 tgtaatgcaa gtagcgtatg cgctcacgca actggtccag aaccttgacc gaacgcagcg
      121 gtggtaacgg cgcagtggcg gttttcatgg cttgttatga ctgttttttt ggggtacagt
      181 ctatgcctcg ggcatccaag cagcaagcgc gttacgccgt gggtcgatgt ttgatgttat
      241 ggagcagcaa cgatgttacg cagcagggca gtcgccctaa aacaaagtta aacatcatgg
      301 gggaagcggt gatcgccgaa gtatcgactc aactatcaga ggtagttggc gtcatcgagc
      361 gccatctcga accgacgttg ctggccgtac atttgtacgg ctccgcagtg gatggcggcc
      421 tgaagccaca cagcgatatt gatttgctgg ttacggtgac cgtaaggctt gatgaaacaa
      481 cgcggcgagc tttgatcaac gaccttttgg aaacttcggc ttcccctgga gagagcgaga
      541 ttctccgcgc tgtagaagtc accattgttg tgcacgacga catcattccg tggcgttatc
      601 cagctaagcg cgaactgcaa tttggagaat ggcagcgcaa tgacattctt gcaggtatct
      661 tcgagccagc cacgatcgac attgatctgg ctatcttgct gacaaaagca agagaacata
      721 gcgttgcctt ggtaggtcca gcggcggagg aactctttga tccggttcct gaacaggatc
      781 tatttgaggc gctaaatgaa accttaacgc tatggaactc gccgcccgac tgggctggcg
      841 atgagcgaaa tgtagtgctt acgttgtccc gcatttggta cagcgcagta accggcaaaa
      901 tcgcgccgaa ggatgtcgct gccgactggg caatggagcg cctgccggcc cagtatcagc
      961 ccgtcatact tgaagctaga caggcttatc ttggacaaga agaagatcgc ttggcctcgc
     1021 gcgcagatca gttggaagaa tttgtccatt acgtgaaagg cgagatcacc aaggtagtcg
     1081 gcaaataatg tctagctaga aattcgttca agccgacgcc gcttcgcggc gcggcttaac
     1141 tcaagcgtta gatgcactaa gcacataatt gctcacagcc aaactatcag gtcaagtctg
     1201 cttttattat ttttaagcgt gcataataag ccctacacaa attgggagat atatcatgct
     1261 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     1321 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     1381 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     1441 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     1501 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     1561 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     1621 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     1681 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     1741 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     1801 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     1861 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     1921 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     1981 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     2041 acggttcctg cactctgtgg tctcattcgt aggcttgatc ctctagcgtc aagcagatcg
     2101 ttcaaacatt tggcaataaa gtttcttaag attgaatcct gttgccggtc ttgcgatgat
     2161 tatcatataa tttctgttga attacgttaa gcatgtaata attaacatgt aatgcatgac
     2221 gttatttatg agatgggttt ttatgattag agtcccgcaa ttatacattt aatacgcgat
     2281 agaaaacaaa atatagcgcg caaactagga taaattatcg cgcgcggtgt catctatgtt
     2341 actagatcga cgct
//
