LOCUS       sequence-377027-        8506 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Barcoded piggybac transposon vector with Dox-inducible expression of
            ZBTB16.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8506)
  AUTHORS   Pierson Smela MD, Kramme CC, Fortuna PRJ, Adams JL, Su R, Dong E, 
            Kobayashi M, Brixi G, Kavirayuni VS, Tysinger E, Kohman RE, Shioda 
            T, Chatterjee P, Church GM
  TITLE     Directed differentiation of human iPSCs to functional ovarian 
            granulosa-like cells via transcription factor overexpression.
  JOURNAL   Elife. 2023 Feb 21;12:e83291. doi: 10.7554/eLife.83291.
  PUBMED    36803359
REFERENCE   2  (bases 1 to 8506)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2023-02-21"; volume: "12:e83291. doi"; pages: " 
            10.7554/eLife.83291"
COMMENT     Sequence Label: pPB-cT3G-cERP2-ZBTB16
FEATURES             Location/Qualifiers
     source          1..8506
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     21..45
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    111..135
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      543..552
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(2164..2188)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2190..2216
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    2383..2517
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     repeat_region   2789..2823
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(2834..2851)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2834..2850)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2843..2865)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(3059..3078)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3180..3202
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3241..3259)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3327..3431
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3432..3500,3501..4292)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3650..3669)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4463..5051
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4952..4971
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5205..5222
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5428..5449
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5464..5469,5470..5487,5488..5494)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5502..5518
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5507..5529
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5526..5542
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5526..5542
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     repeat_region   5568..5630
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     polyA_signal    5806..5887
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(6604..6660)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(6670..7413)
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     CDS             complement(7414..7437)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      7437..7446
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(7466..7677)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     enhancer        7678..8057
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     protein_bind    8266..8284
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8302..8320
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8338..8356
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8374..8392
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8410..8428
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8446..8464
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8482..8500
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 aagcttgcct atataagcag agctcgttta gtgaaccgtc agatcgcctg gagacgccat
       61 ccacgctgtt ttgacctcca tagaagacac cgggaccgat ccagcctacc acaagtttgt
      121 acaaaaaagc aggctccacc atggatctga caaaaatggg catgatccag ctgcagaacc
      181 ctagccaccc cacggggcta ctgtgcaagg ccaaccagat gcggctggcc gggactttgt
      241 gcgatgtggt catcatggtg gacagccagg agttccacgc ccaccggacg gtgctggcct
      301 gcaccagcaa gatgtttgag atcctcttcc accgcaatag tcaacactat actttggact
      361 tcctctcgcc aaagaccttc cagcagattc tggagtatgc atatacagcc acgctgcaag
      421 ccaaggcgga ggacctggat gacctgctgt atgcggccga gatcctggag atcgagtacc
      481 tggaggaaca gtgcctgaag atgctggaga ccatccaggc ctcagacgac aatgacacgg
      541 aggccaccat ggccgatggc ggggccgagg aagaagagga ccgcaaggct cggtacctca
      601 agaacatctt catctcgaag cattccagcg aggagagtgg gtatgccagt gtggctggac
      661 agagcctccc tgggcccatg gtggaccaga gcccttcagt ctccacttca tttggtcttt
      721 cagccatgag tcccaccaag gctgcagtgg acagtttgat gaccatagga cagtctctcc
      781 tgcagggaac tcttcagcca cctgcagggc ccgaggagcc aactctggct gggggtgggc
      841 ggcaccctgg ggtggctgag gtgaagacgg agatgatgca ggtggatgag gtgcccagcc
      901 aggacagccc tggggcagcc gagtccagca tctcaggagg gatgggggac aaggttgagg
      961 aaagaggcaa agaggggcct gggaccccga ctcgaagcag cgtcatcacc agtgctaggg
     1021 agctacacta tgggcgagag gagagtgccg agcaggtgcc acccccagct gaggctggcc
     1081 aggcccccac tggccgacct gagcacccag cacccccgcc tgagaagcat ctgggcatct
     1141 actccgtgtt gcccaaccac aaggctgacg ctgtattgag catgccgtct tccgtgacct
     1201 ctggcctcca cgtgcagcct gccctggctg tctccatgga cttcagcacc tatggggggc
     1261 tgctgcccca gggcttcatc cagagggagc tgttcagcaa gctgggggag ctggctgtgg
     1321 gcatgaagtc agagagccgg accatcggag agcagtgcag cgtgtgtggg gtcgagcttc
     1381 ctgataacga ggctgtggag cagcacagga agctgcacag tgggatgaag acgtacgggt
     1441 gcgagctctg cgggaagcgg ttcctggata gtttgcggct gagaatgcac ttactggctc
     1501 attcagcggg tgccaaagcc tttgtctgtg atcagtgcgg tgcacagttt tcgaaggagg
     1561 atgccctgga gacacacagg cagacccata ctggcactga catggccgtc ttctgtctgc
     1621 tgtgtgggaa gcgcttccag gcgcagagcg cactgcagca gcacatggag gtccacgcgg
     1681 gcgtgcgcag ctacatctgc agtgagtgca accgcacctt ccccagccac acggctctca
     1741 aacgccacct gcgctcacat acaggcgacc acccctacga gtgtgagttc tgtggcagct
     1801 gcttccggga tgagagcaca ctcaagagcc acaaacgcat ccacacgggt gagaaaccct
     1861 acgagtgcaa tggctgtggc aagaagttca gcctcaagca tcagctggag acgcactata
     1921 gggtgcacac aggtgagaag ccctttgagt gtaagctctg ccaccagcgc tcccgggact
     1981 actcggccat gatcaagcac ctgagaacgc acaacggcgc ctcgccctac cagtgcacca
     2041 tctgcacaga gtactgcccc agcctctcct ccatgcagaa gcacatgaag ggccacaagc
     2101 ccgaggagat cccgcccgac tggaggatag agaagacgta cctctacctg tgctatgtgt
     2161 tggacccagc tttcttgtac aaagtggtat acccatacga tgttccagat tacgcttagt
     2221 aatgagaaat actcagaaga tgtcactcac catagtccgc agtgaaagat aaatgatctt
     2281 gcgccaccat tgtcatttgt tttagagcta gaaatagcaa gtaggccact gagcaagaac
     2341 tatgaatgag atctttagac atattgcggc cgctttctcc gcgatccaga catgataaga
     2401 tacattgatg agtttggaca aaccaaaact agaatgcagt gaaaaaaatg ccttatttgt
     2461 gaaatttgtg atgctattgc cttatttgta accattataa gctgcaataa acaagttatc
     2521 tataacaaga aaatatatat ataataagtt atcacgtaag tagaacatga aataacaata
     2581 taattatcgt atgagttaaa tcttaaaagt cacgtaaaag ataatcatgc gtcattttga
     2641 ctcacgcggt cgttatagtt caaaatcagt gacacttacc gcattgacaa gcacgcctca
     2701 cgggagctcc aagcggcgac tgagatgtcc taaatgcaca gcgacggatt cgcgctattt
     2761 agaaagagag agcaatattt caagaatgca tgcgtcaatt ttacgcagac tatctttcta
     2821 gggttaagaa ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta
     2881 cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg
     2941 cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg cgcctgatgc
     3001 ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatggtgc actctcagta
     3061 caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg
     3121 cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accatgtctc
     3181 cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacacgcga gttcgaaagg
     3241 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt
     3301 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     3361 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     3421 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     3481 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     3541 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     3601 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     3661 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     3721 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     3781 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     3841 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     3901 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     3961 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     4021 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     4081 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     4141 agcgtggttc acgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     4201 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     4261 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     4321 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     4381 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     4441 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     4501 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     4561 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     4621 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     4681 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     4741 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     4801 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     4861 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     4921 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     4981 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     5041 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     5101 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     5161 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     5221 aggaagcgga tgagcgccca atacgcaaac cgcctctcac cgcgcgttgg ccgattcatt
     5281 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaaacc
     5341 gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg
     5401 gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca
     5461 ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt
     5521 tcacacagga aacagctatg accatgatta cgccaaggtc gacttaaccc tagaaagata
     5581 atcatattgt gacgtacgtt aaagataatc atgcgtaaaa ttgacgcatg tgttttatcg
     5641 gtctgtatat cgaggtttat ttattaattt gaatagatat taagttttat tatatttaca
     5701 cttacatact aataataaat tcaacaaaca atttatttat gtttatttat ttattaaaaa
     5761 aaaacaaaaa ctcaaaattt cttctataaa gtaacaaaac ttttagcagt gaaaaaaatg
     5821 ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa
     5881 acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga
     5941 ggttttttaa agcaagtaaa acctctacaa atgtggtatg gctgattatg atcctctgga
     6001 gattcatgcg ccaggttttc tggtcatgca ccacgttcgt gggccctcag gaacctccac
     6061 gtcagcggta actgtgaagc ccagtcgctc gtaaaaagga agattccggg gagcactggt
     6121 ttcgaggaac gccggcacgc cagcccgttc cgcggcctca acaccaggaa gaacgacagc
     6181 gcttcccaaa cctttacctt ggtgatctgg gctgacaccg acagtggcaa gaaaccacgc
     6241 aggctccttt ggtctatgag gagccagcaa gccctccatt tgctgttgtg ccgccaacct
     6301 actccctgac agctcggcca tccgcggacc tatttccgcg aacactgcgc cggcttcgac
     6361 gctttccgga gtagtccaga ccgcaactgc ggcaccatcg tccgcaaccc aaactttacc
     6421 aatgtccaga cccacgcgcg taagaaacag ctcttgcaac tcagttaccc tctctatgtg
     6481 tctgtccgga tctacggtat ggcgggtcgc tggataatct gcaaatgctg cggcaagtgt
     6541 tcggacagcc cgtggtacat cgtcacgggt agcgagccta acagtgggct tatattccgt
     6601 catggggccg gggttctcct ccacgtcgcc ggcctgcttc agcaggctga agttggtggc
     6661 gccgctgccc ccggggagca tgtcaaggtc aaaatcgtca agagcgtcag caggcagcat
     6721 atcaaggtca aagtcgtcaa gggcatcggc tgggagcatg tctaagtcaa aatcgtcaag
     6781 ggcgtcggtc ggcccgccgc tttcgcactt tagctgtttc tccaggccac atatgattag
     6841 ttccaggccg aaaaggaagg caggttcggc tccctgccgg tcgaacagct caattgcttg
     6901 tttcagaagt gggggcatag aatcggtggt aggtgtctct ctttcctctt ttgctacttg
     6961 atgctcctgt tcctccaata cgcagcccag tgtaaagtgg cccacggcgg acagagcgta
     7021 cagtgcgttc tccagggaga agccttgctg acacaggaac gcgagctgat tttccagggt
     7081 ttcgtactgt ttctctgttg ggcgggtgcc gagatgcact ttagccccgt cacgatgtga
     7141 gaggagagca cagcggtatg acttggcgtt gttccgcaga aagtcttgcc atgactcgcc
     7201 ttccaggggg caggagtggg tatgatgcct gtccagcatc tcgattggca gggcatcgag
     7261 cagggcccgc ttgttcttca cgtgccagta cagggtaggc tgctcaactc ccagcttttg
     7321 agcgagtttc cttgtcgtca ggccttcgat accgactcca ttgagtaatt ccagagcaga
     7381 gtttatgact ttgctcttgt ccagtctaga catcttatcg tcatcgtctt tgtaatccat
     7441 ggtggcggat cccgcgtcac gacacctgtg ttctggcggc aaacccgttg cgaaaaagaa
     7501 cgttcacggc gactactgca cttatatacg gttctccccc accctcggga aaaaggcgga
     7561 gccagtacac gacatcactt tcccagttta ccccgcgcca ccttctctag gcaccggttc
     7621 aattgccgac ccctcccccc aacttctcgg gcactgtggg ccatgtgcgc tctgccccat
     7681 ggtaatagcc atgactaata cgtagatgta ctgccaagta ggaaagtgcc ataaggtcat
     7741 gtactgggca taatgccagg cgggccattt accgtcattg acgtcaatag ggggcgtact
     7801 tggcatatga tacacttgat gtactgccaa gtgggcagtt taccgtaaat actccaccca
     7861 ttgacgtcaa tggaaagtgc ctattggcgt tactatggga acatacgtca ttattgacgt
     7921 caatgggcgg gggtcgttgg gcggtcagcc aggcgggcca tttaccgtaa gttatgtaac
     7981 gcggaactcc atatatgggc tatgaactaa tgaccccgta attgattact attaataact
     8041 agtcaataat caatgtcata agctctaatc gcataatcga gctaagagcg attaatatgc
     8101 ttagctagct tagctaagct agatttctct attctcccac aatatatagc agctattagc
     8161 ttagcagtcc gaatacgatc gtccacgatc cataagcgta acacatttga gtagcgactg
     8221 acgggcaccg gagccactcg agtggaatta tcacctcgag tttactccct atcagtgata
     8281 gagaacgtat gaagagttta ctccctatca gtgatagaga acgtatgcag actttactcc
     8341 ctatcagtga tagagaacgt ataaggagtt tactccctat cagtgataga gaacgtatga
     8401 ccagtttact ccctatcagt gatagagaac gtatctacag tttactccct atcagtgata
     8461 gagaacgtat atccagttta ctccctatca gtgatagaga acgtat
//
