LOCUS       sequence-377029-       11183 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of S. aureus dead Cas9 (dCas9/dSaCas9/SadCas9)
            fused to the KRAB domain for gene silencing and the blasticidin 
            resistance gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11183)
  AUTHORS   Sapozhnikov DM, Szyf M
  TITLE     The PROTECTOR strategy employs dCas orthologs to sterically shield 
            off-target sites from CRISPR/Cas activity.
  JOURNAL   Sci Rep. 2023 Feb 9;13(1):2280. doi: 10.1038/s41598-023-29332-2.
  PUBMED    36759683
REFERENCE   2  (bases 1 to 11183)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2023-02-9"; volume: "13(1):2280. doi"; pages: " 
            10.1038/s41598-023-29332-2"
COMMENT     Sequence Label: pLenti-dSaCas9-KRAB-blast
FEATURES             Location/Qualifiers
     source          1..11183
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     13..30
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    147..168
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(183..188,189..206,207..213)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    221..237
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     226..248
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     245..261
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     245..261
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     280..300
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        282..300
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        328..554
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             555..735
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    782..907
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1400..1633
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1818..1862
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2129..2246
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2269..2572
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2573..2776
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2727..2747
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             2804..2830
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             2834..2854
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6035..6082
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             6116..6310
                     /codon_start=1
                     /product="Kruppel-associated box (KRAB) transcriptional 
                     repression domain from the human zinc finger protein ZNF10 
                     (Margolin et al., 1994)"
                     /label=KRAB
                     /translation="RTLVTFKDVFVDFTREEWKLLDTAQQILYRNVMLENYKNLVSLGY
                     QLTKPDVILRLEKGEEPWLV"
     CDS             6377..6397
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6446..6487
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    6511..7099
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6564..6584)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6982..6993)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        7114..7430
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7281..7416
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        7478..7525
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7544..7942
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             8029..8262
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8334..8455
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8371..8390)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8425..8444
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8495..8630
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8650..8669)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8651..8669)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8679..8696)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8679..8695)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8688..8710)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8837..9292
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8924..8943)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9134..9155
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9318..9422
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9423..9491,9492..10283)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9641..9660)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10454..11042
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10943..10962
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
       61 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg
      121 aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag
      181 gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt
      241 cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc tcactaaagg
      301 gaacaaaagc tggagctgca agcttaatgt agtcttatgc aatactcttg tagtcttgca
      361 acatggtaac gatgagttag caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc
      421 gattggtgga agtaaggtgg tacgatcgtg ccttattagg aaggcaacag acgggtctga
      481 catggattgg acgaaccact gaattgccgc attgcagaga tattgtattt aagtgcctag
      541 ctcgatacat aaacgggtct ctctggttag accagatctg agcctgggag ctctctggct
      601 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
      661 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
      721 ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga
      781 gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg
      841 actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga
      901 gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc
      961 agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg
     1021 attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca
     1081 gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc
     1141 aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa
     1201 gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg ctgatcttca
     1261 gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa tataaagtag
     1321 taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag
     1381 aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca
     1441 ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag
     1501 tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca
     1561 cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg
     1621 atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc
     1681 cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga
     1741 tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat
     1801 cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt
     1861 tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag
     1921 taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg aatagagtta
     1981 ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggacccgaca
     2041 ggcccgaagg aatagaagaa gaaggtggag agagagacag agacagatcc attcgattag
     2101 tgaacggatc tcgacggtat cggttaactt ttaaaagaaa aggggggatt ggggggtaca
     2161 gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa
     2221 aacaaattac aaaaattcaa aattttatcg ataagcttgg gagttccgcg ttacataact
     2281 tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat
     2341 gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta
     2401 tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc
     2461 tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg
     2521 ggactttcct acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg
     2581 gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct
     2641 ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa
     2701 atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt
     2761 ctatataagc agagctctct ggctaactac cggtgccacc atgtacccat acgatgttcc
     2821 agattacgct gccccaaaga agaagcggaa ggtcggtatc cacggagtcc cagcagccaa
     2881 gcggaactac atcctgggcc tggccatcgg catcaccagc gtgggctacg gcatcatcga
     2941 ctacgagaca cgggacgtga tcgatgccgg cgtgcggctg ttcaaagagg ccaacgtgga
     3001 aaacaacgag ggcaggcgga gcaagagagg cgccagaagg ctgaagcggc ggaggcggca
     3061 tagaatccag agagtgaaga agctgctgtt cgactacaac ctgctgaccg accacagcga
     3121 gctgagcggc atcaacccct acgaggccag agtgaagggc ctgagccaga agctgagcga
     3181 ggaagagttc tctgccgccc tgctgcacct ggccaagaga agaggcgtgc acaacgtgaa
     3241 cgaggtggaa gaggacaccg gcaacgagct gtccaccaaa gagcagatca gccggaacag
     3301 caaggccctg gaagagaaat acgtggccga actgcagctg gaacggctga agaaagacgg
     3361 cgaagtgcgg ggcagcatca acagattcaa gaccagcgac tacgtgaaag aagccaaaca
     3421 gctgctgaag gtgcagaagg cctaccacca gctggaccag agcttcatcg acacctacat
     3481 cgacctgctg gaaacccggc ggacctacta tgagggacct ggcgagggca gccccttcgg
     3541 ctggaaggac atcaaagaat ggtacgagat gctgatgggc cactgcacct acttccccga
     3601 ggaactgcgg agcgtgaagt acgcctacaa cgccgacctg tacaacgccc tgaacgacct
     3661 gaacaatctc gtgatcacca gggacgagaa cgagaagctg gaatattacg agaagttcca
     3721 gatcatcgag aacgtgttca agcagaagaa gaagcccacc ctgaagcaga tcgccaaaga
     3781 aatcctcgtg aacgaagagg atattaaggg ctacagagtg accagcaccg gcaagcccga
     3841 gttcaccaac ctgaaggtgt accacgacat caaggacatt accgcccgga aagagattat
     3901 tgagaacgcc gagctgctgg atcagattgc caagatcctg accatctacc agagcagcga
     3961 ggacatccag gaagaactga ccaatctgaa ctccgagctg acccaggaag agatcgagca
     4021 gatctctaat ctgaagggct ataccggcac ccacaacctg agcctgaagg ccatcaacct
     4081 gatcctggac gagctgtggc acaccaacga caaccagatc gctatcttca accggctgaa
     4141 gctggtgccc aagaaggtgg acctgtccca gcagaaagag atccccacca ccctggtgga
     4201 cgacttcatc ctgagccccg tcgtgaagag aagcttcatc cagagcatca aagtgatcaa
     4261 cgccatcatc aagaagtacg gcctgcccaa cgacatcatt atcgagctgg cccgcgagaa
     4321 gaactccaag gacgcccaga aaatgatcaa cgagatgcag aagcggaacc ggcagaccaa
     4381 cgagcggatc gaggaaatca tccggaccac cggcaaagag aacgccaagt acctgatcga
     4441 gaagatcaag ctgcacgaca tgcaggaagg caagtgcctg tacagcctgg aagccatccc
     4501 tctggaagat ctgctgaaca accccttcaa ctatgaggtg gaccacatca tccccagaag
     4561 cgtgtccttc gacaacagct tcaacaacaa ggtgctcgtg aagcaggaag aagccagcaa
     4621 gaagggcaac cggaccccat tccagtacct gagcagcagc gacagcaaga tcagctacga
     4681 aaccttcaag aagcacatcc tgaatctggc caagggcaag ggcagaatca gcaagaccaa
     4741 gaaagagtat ctgctggaag aacgggacat caacaggttc tccgtgcaga aagacttcat
     4801 caaccggaac ctggtggata ccagatacgc caccagaggc ctgatgaacc tgctgcggag
     4861 ctacttcaga gtgaacaacc tggacgtgaa agtgaagtcc atcaatggcg gcttcaccag
     4921 ctttctgcgg cggaagtgga agtttaagaa agagcggaac aaggggtaca agcaccacgc
     4981 cgaggacgcc ctgatcattg ccaacgccga tttcatcttc aaagagtgga agaaactgga
     5041 caaggccaaa aaagtgatgg aaaaccagat gttcgaggaa aagcaggccg agagcatgcc
     5101 cgagatcgaa accgagcagg agtacaaaga gatcttcatc accccccacc agatcaagca
     5161 cattaaggac ttcaaggact acaagtacag ccaccgggtg gacaagaagc ctaatagaga
     5221 gctgattaac gacaccctgt actccacccg gaaggacgac aagggcaaca ccctgatcgt
     5281 gaacaatctg aacggcctgt acgacaagga caatgacaag ctgaaaaagc tgatcaacaa
     5341 gagccccgaa aagctgctga tgtaccacca cgacccccag acctaccaga aactgaagct
     5401 gattatggaa cagtacggcg acgagaagaa tcccctgtac aagtactacg aggaaaccgg
     5461 gaactacctg accaagtact ccaaaaagga caacggcccc gtgatcaaga agattaagta
     5521 ttacggcaac aaactgaacg cccatctgga catcaccgac gactacccca acagcagaaa
     5581 caaggtcgtg aagctgtccc tgaagcccta cagattcgac gtgtacctgg acaatggcgt
     5641 gtacaagttc gtgaccgtga agaatctgga tgtgatcaaa aaagaaaact actacgaagt
     5701 gaatagcaag tgctatgagg aagctaagaa gctgaagaag atcagcaacc aggccgagtt
     5761 tatcgcctcc ttctacaaca acgatctgat caagatcaac ggcgagctgt atagagtgat
     5821 cggcgtgaac aacgacctgc tgaaccggat cgaagtgaac atgatcgaca tcacctaccg
     5881 cgagtacctg gaaaacatga acgacaagag gccccccagg atcattaaga caatcgcctc
     5941 caagacccag agcattaaga agtacagcac agacattctg ggcaacctgt atgaagtgaa
     6001 atctaagaag caccctcaga tcatcaaaaa gggcaaaagg ccggcggcca cgaaaaaggc
     6061 cggccaggca aaaaagaaaa agggatccga tgctaagtca ctgactgcct ggtcccggac
     6121 actggtgacc ttcaaggatg tgtttgtgga cttcaccagg gaggagtgga agctgctgga
     6181 cactgctcag cagatcctgt acagaaatgt gatgctggag aactataaga acctggtttc
     6241 cttgggttat cagcttacta agccagatgt gatcctccgg ttggagaagg gagaagagcc
     6301 ctggctggtg gagagagaaa ttcaccaaga gacccatcct gattcagaga ctgcatttga
     6361 aatcaaatca tcagttccga aaaagaaacg caaagtttaa gaattcgttc cggagtcgtc
     6421 gactctagag ggcccgcggt tcgaaggtaa gcctatccct aaccctctcc tcggtctcga
     6481 ttctacgcgt accggttagt aatgatcgac aatcaacctc tggattacaa aatttgtgaa
     6541 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
     6601 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
     6661 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
     6721 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
     6781 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
     6841 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
     6901 gggaagctga cgtcctttcc atggctgctc gcctgtgttg ccacctggat tctgcgcggg
     6961 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
     7021 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc
     7081 ctttgggccg cctccccgcc tggcgatggt accggtgtgg aaagtcccca ggctccccag
     7141 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc
     7201 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag
     7261 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     7321 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc
     7381 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg
     7441 gagcttgtat atccattttc ggatctgatc agcacgtgtt gacaattaat catcggcata
     7501 gtatatcggc atagtataat acgacaaggt gaggaactaa accatggcca agcctttgtc
     7561 tcaagaagaa tccaccctca ttgaaagagc aacggctaca atcaacagca tccccatctc
     7621 tgaagactac agcgtcgcca gcgcagctct ctctagcgac ggccgcatct tcactggtgt
     7681 caatgtatat cattttactg ggggaccttg tgcagaactc gtggtgctgg gcactgctgc
     7741 tgctgcggca gctggcaacc tgacttgtat cgtcgcgatc ggaaatgaga acaggggcat
     7801 cttgagcccc tgcggacggt gccgacaggt gcttctcgat ctgcatcctg ggatcaaagc
     7861 catagtgaag gacagtgatg gacagccgac ggcagttggg attcgtgaat tgctgccctc
     7921 tggttatgtg tgggagggct aagcacaatt cgagctcggt acctttaaga ccaatgactt
     7981 acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa
     8041 ttcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc tggttagacc
     8101 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     8161 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     8221 gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc
     8281 ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt
     8341 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     8401 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     8461 tctggctcta gctatcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     8521 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     8581 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc tagggacgta
     8641 cccaattcgc cctatagtga gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt
     8701 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
     8761 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
     8821 ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     8881 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     8941 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     9001 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
     9061 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     9121 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
     9181 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
     9241 atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat ttaggtggca
     9301 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     9361 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     9421 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     9481 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     9541 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     9601 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     9661 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     9721 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     9781 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     9841 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     9901 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     9961 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
    10021 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
    10081 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
    10141 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
    10201 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
    10261 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
    10321 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
    10381 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
    10441 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
    10501 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
    10561 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
    10621 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
    10681 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
    10741 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
    10801 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
    10861 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
    10921 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
    10981 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
    11041 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
    11101 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
    11161 ctgataccgc tcgccgcagc cga
//
