LOCUS       sequence-377031-        8272 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Barcoded piggybac transposon vector with Dox-inducible expression of
            NR4A1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8272)
  AUTHORS   Pierson Smela MD, Kramme CC, Fortuna PRJ, Adams JL, Su R, Dong E, 
            Kobayashi M, Brixi G, Kavirayuni VS, Tysinger E, Kohman RE, Shioda 
            T, Chatterjee P, Church GM
  TITLE     Directed differentiation of human iPSCs to functional ovarian 
            granulosa-like cells via transcription factor overexpression.
  JOURNAL   Elife. 2023 Feb 21;12:e83291. doi: 10.7554/eLife.83291.
  PUBMED    36803359
REFERENCE   2  (bases 1 to 8272)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2023-02-21"; volume: "12:e83291. doi"; pages: " 
            10.7554/eLife.83291"
COMMENT     Sequence Label: pPB-cT3G-cERP2-NR4A1
FEATURES             Location/Qualifiers
     source          1..8272
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     22..46
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    112..136
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1931..1955)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1957..1983
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    2150..2284
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     repeat_region   2556..2590
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(2601..2618)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2601..2617)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2610..2632)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(2826..2845)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2947..2969
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3008..3026)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3094..3198
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3199..3267,3268..4059)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3417..3436)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4230..4818
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4719..4738
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4972..4989
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5195..5216
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5231..5236,5237..5254,5255..5261)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5269..5285
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5274..5296
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5293..5309
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5293..5309
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     repeat_region   5335..5397
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     polyA_signal    5573..5654
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(6371..6427)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(6437..7180)
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     CDS             complement(7181..7204)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      7204..7213
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(7233..7444)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     enhancer        7445..7824
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     protein_bind    8033..8051
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8069..8087
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8105..8123
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8141..8159
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8177..8195
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8213..8231
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8249..8267
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 taagcttgcc tatataagca gagctcgttt agtgaaccgt cagatcgcct ggagacgcca
       61 tccacgctgt tttgacctcc atagaagaca ccgggaccga tccagcctac cacaagtttg
      121 tacaaaaaag ttggcatgcc ctgtatccaa gcccaatatg ggacaccagc accgagtccg
      181 ggaccccgtg accacctggc aagcgacccc ctgacccctg agttcatcaa gcccaccatg
      241 gacctggcca gccccgaggc agcccccgct gcccccactg ccctgcccag cttcagcacc
      301 ttcatggacg gctacacagg agagtttgac accttcctct accagctgcc aggaacagtc
      361 cagccatgct cctcagcctc ctcctcggcc tcctccacat cctcgtcctc agccacctcc
      421 cctgcctctg cctccttcaa gttcgaggac ttccaggtgt acggctgcta ccccggcccc
      481 ctgagcggcc cagtggatga ggccctgtcc tccagtggct ctgactacta tggcagcccc
      541 tgctcggccc cgtcgccctc cacgcccagc ttccagccgc cccagctctc tccctgggat
      601 ggctccttcg gccacttctc gcccagccag acttacgaag gcctgcgggc atggacagag
      661 cagctgccca aagcctctgg gcccccacag cctccagcct tcttttcctt cagtcctccc
      721 accggcccca gccccagcct ggcccagagc cccctgaagt tgttcccctc acaggccacc
      781 caccagctgg gggagggaga gagctattcc atgcctacgg ccttcccagg tttggcaccc
      841 acttctccac accttgaggg ctcggggata ctggatacac ccgtgacctc aaccaaggcc
      901 cggagcgggg ccccaggtgg aagtgaaggc cgctgtgctg tgtgtgggga caacgcttca
      961 tgccagcatt atggtgtccg cacatgtgag ggctgcaagg gcttcttcaa gcgcacagtg
     1021 cagaaaaacg ccaagtacat ctgcctggct aacaaggact gccctgtgga caagaggcgg
     1081 cgaaaccgct gccagttctg ccgcttccag aagtgcctgg cggtgggcat ggtgaaggaa
     1141 gttgtccgaa cagacagcct gaaggggcgg cggggccggc taccttcaaa acccaagcag
     1201 cccccagatg cctcccctgc caatctcctc acttccctgg tccgtgcaca cctggactca
     1261 gggcccagca ctgccaaact ggactactcc aagttccagg agctggtgct gccccacttt
     1321 gggaaggaag atgctgggga tgtacagcag ttctacgacc tgctctccgg ttctctggag
     1381 gtcatccgca agtgggcgga gaagatccct ggctttgctg agctgtcacc ggctgaccag
     1441 gacctgttgc tggagtcggc cttcctggag ctcttcatcc tccgcctggc gtacaggtct
     1501 aagccaggcg agggcaagct catcttctgc tcaggcctgg tgctacaccg gctgcagtgt
     1561 gcccgtggct tcggggactg gattgacagt atcctggcct tctcaaggtc cctgcacagc
     1621 ttgcttgtcg atgtccctgc cttcgcctgc ctctctgccc ttgtcctcat caccgaccgg
     1681 catgggctgc aggagccgcg gcgggtggag gagctgcaga accgcatcgc cagctgcctg
     1741 aaggagcacg tggcagctgt ggcgggcgag ccccagccag ccagctgcct gtcacgtctg
     1801 ttgggcaaac tgcccgagct gcggaccctg tgcacccagg gcctgcagcg catcttctac
     1861 ctcaagctgg aggacttggt gccccctcca cccatcattg acaagatctt catggacacg
     1921 ctgcccttct gcccaacttt cttgtacaaa gtggtatacc catacgatgt tccagattac
     1981 gcttagtaat gagaaatact cagaagatgt cactcaccat agtccgcagt gaaagataaa
     2041 tgatcatgga gcaattgtac aaatcgtttt agagctagaa atagcaagta ggccactgag
     2101 caagaactat gaatgagatc tttagacata ttgcggccgc tttctccgcg atccagacat
     2161 gataagatac attgatgagt ttggacaaac caaaactaga atgcagtgaa aaaaatgcct
     2221 tatttgtgaa atttgtgatg ctattgcctt atttgtaacc attataagct gcaataaaca
     2281 agttatctat aacaagaaaa tatatatata ataagttatc acgtaagtag aacatgaaat
     2341 aacaatataa ttatcgtatg agttaaatct taaaagtcac gtaaaagata atcatgcgtc
     2401 attttgactc acgcggtcgt tatagttcaa aatcagtgac acttaccgca ttgacaagca
     2461 cgcctcacgg gagctccaag cggcgactga gatgtcctaa atgcacagcg acggattcgc
     2521 gctatttaga aagagagagc aatatttcaa gaatgcatgc gtcaatttta cgcagactat
     2581 ctttctaggg ttaagaattc actggccgtc gttttacaac gtcgtgactg ggaaaaccct
     2641 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc
     2701 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc
     2761 ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atggtgcact
     2821 ctcagtacaa tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc
     2881 gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc
     2941 atgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cacgcgagtt
     3001 cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct
     3061 tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     3121 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     3181 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     3241 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     3301 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     3361 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
     3421 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
     3481 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
     3541 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
     3601 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     3661 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     3721 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
     3781 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
     3841 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     3901 gccggtgagc gtggttcacg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     3961 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     4021 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
     4081 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     4141 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     4201 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     4261 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     4321 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     4381 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     4441 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     4501 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     4561 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     4621 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     4681 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     4741 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     4801 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     4861 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     4921 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     4981 gtgagcgagg aagcggatga gcgcccaata cgcaaaccgc ctctcaccgc gcgttggccg
     5041 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
     5101 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
     5161 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg
     5221 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat
     5281 aacaatttca cacaggaaac agctatgacc atgattacgc caaggtcgac ttaaccctag
     5341 aaagataatc atattgtgac gtacgttaaa gataatcatg cgtaaaattg acgcatgtgt
     5401 tttatcggtc tgtatatcga ggtttattta ttaatttgaa tagatattaa gttttattat
     5461 atttacactt acatactaat aataaattca acaaacaatt tatttatgtt tatttattta
     5521 ttaaaaaaaa acaaaaactc aaaatttctt ctataaagta acaaaacttt tagcagtgaa
     5581 aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct
     5641 gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg
     5701 tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg tggtatggct gattatgatc
     5761 ctctggagat tcatgcgcca ggttttctgg tcatgcacca cgttcgtggg ccctcaggaa
     5821 cctccacgtc agcggtaact gtgaagccca gtcgctcgta aaaaggaaga ttccggggag
     5881 cactggtttc gaggaacgcc ggcacgccag cccgttccgc ggcctcaaca ccaggaagaa
     5941 cgacagcgct tcccaaacct ttaccttggt gatctgggct gacaccgaca gtggcaagaa
     6001 accacgcagg ctcctttggt ctatgaggag ccagcaagcc ctccatttgc tgttgtgccg
     6061 ccaacctact ccctgacagc tcggccatcc gcggacctat ttccgcgaac actgcgccgg
     6121 cttcgacgct ttccggagta gtccagaccg caactgcggc accatcgtcc gcaacccaaa
     6181 ctttaccaat gtccagaccc acgcgcgtaa gaaacagctc ttgcaactca gttaccctct
     6241 ctatgtgtct gtccggatct acggtatggc gggtcgctgg ataatctgca aatgctgcgg
     6301 caagtgttcg gacagcccgt ggtacatcgt cacgggtagc gagcctaaca gtgggcttat
     6361 attccgtcat ggggccgggg ttctcctcca cgtcgccggc ctgcttcagc aggctgaagt
     6421 tggtggcgcc gctgcccccg gggagcatgt caaggtcaaa atcgtcaaga gcgtcagcag
     6481 gcagcatatc aaggtcaaag tcgtcaaggg catcggctgg gagcatgtct aagtcaaaat
     6541 cgtcaagggc gtcggtcggc ccgccgcttt cgcactttag ctgtttctcc aggccacata
     6601 tgattagttc caggccgaaa aggaaggcag gttcggctcc ctgccggtcg aacagctcaa
     6661 ttgcttgttt cagaagtggg ggcatagaat cggtggtagg tgtctctctt tcctcttttg
     6721 ctacttgatg ctcctgttcc tccaatacgc agcccagtgt aaagtggccc acggcggaca
     6781 gagcgtacag tgcgttctcc agggagaagc cttgctgaca caggaacgcg agctgatttt
     6841 ccagggtttc gtactgtttc tctgttgggc gggtgccgag atgcacttta gccccgtcac
     6901 gatgtgagag gagagcacag cggtatgact tggcgttgtt ccgcagaaag tcttgccatg
     6961 actcgccttc cagggggcag gagtgggtat gatgcctgtc cagcatctcg attggcaggg
     7021 catcgagcag ggcccgcttg ttcttcacgt gccagtacag ggtaggctgc tcaactccca
     7081 gcttttgagc gagtttcctt gtcgtcaggc cttcgatacc gactccattg agtaattcca
     7141 gagcagagtt tatgactttg ctcttgtcca gtctagacat cttatcgtca tcgtctttgt
     7201 aatccatggt ggcggatccc gcgtcacgac acctgtgttc tggcggcaaa cccgttgcga
     7261 aaaagaacgt tcacggcgac tactgcactt atatacggtt ctcccccacc ctcgggaaaa
     7321 aggcggagcc agtacacgac atcactttcc cagtttaccc cgcgccacct tctctaggca
     7381 ccggttcaat tgccgacccc tccccccaac ttctcgggca ctgtgggcca tgtgcgctct
     7441 gccccatggt aatagccatg actaatacgt agatgtactg ccaagtagga aagtgccata
     7501 aggtcatgta ctgggcataa tgccaggcgg gccatttacc gtcattgacg tcaatagggg
     7561 gcgtacttgg catatgatac acttgatgta ctgccaagtg ggcagtttac cgtaaatact
     7621 ccacccattg acgtcaatgg aaagtgccta ttggcgttac tatgggaaca tacgtcatta
     7681 ttgacgtcaa tgggcggggg tcgttgggcg gtcagccagg cgggccattt accgtaagtt
     7741 atgtaacgcg gaactccata tatgggctat gaactaatga ccccgtaatt gattactatt
     7801 aataactagt caataatcaa tgtcataagc tctaatcgca taatcgagct aagagcgatt
     7861 aatatgctta gctagcttag ctaagctaga tttctctatt ctcccacaat atatagcagc
     7921 tattagctta gcagtccgaa tacgatcgtc cacgatccat aagcgtaaca catttgagta
     7981 gcgactgacg ggcaccggag ccactcgagt ggaattatca cctcgagttt actccctatc
     8041 agtgatagag aacgtatgaa gagtttactc cctatcagtg atagagaacg tatgcagact
     8101 ttactcccta tcagtgatag agaacgtata aggagtttac tccctatcag tgatagagaa
     8161 cgtatgacca gtttactccc tatcagtgat agagaacgta tctacagttt actccctatc
     8221 agtgatagag aacgtatatc cagtttactc cctatcagtg atagagaacg ta
//
