LOCUS       sequence-377041-       11033 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Knock-in gene targeting in marmoset cells at TBXT locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11033)
  AUTHORS   Yoshimatsu S, Kisu I, Qian E, Noce T
  TITLE     A New Horizon in Reproductive Research with Pluripotent Stem Cells: 
            Successful In Vitro Gametogenesis in Rodents, Its Application to 
            Large Animals, and Future In Vitro Reconstitution of Reproductive 
            Organs Such as ""Uteroid"" and ""Oviductoid"".
  JOURNAL   Biology (Basel). 2022 Jun 29;11(7):987. doi: 
            10.3390/biology11070987.
  PUBMED    36101367
REFERENCE   2  (bases 1 to 11033)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biology 
            (Basel)."; date: "2022-06-29"; volume: "11(7):987. doi"; pages: " 
            10.3390/biology11070987"
COMMENT     Sequence Label: pKI-cjT-tdTomato-PGK-Neo
FEATURES             Location/Qualifiers
     source          1..11033
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     14..31
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    148..169
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(184..189,190..207,208..214)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    222..238
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     227..249
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     246..262
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     246..262
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             1241..1297
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             1298..2728
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     polyA_signal    2850..2971
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2887..2906)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2941..2960
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    3011..3044
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        3088..3587
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(3123..3146)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     3520..3539
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             3601..4404
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     protein_bind    4732..4765
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             6180..6752
                     /codon_start=1
                     /gene="Corynebacterium diphtheriae tox"
                     /product="diphtheria toxin fragment A"
                     /label=DTA
                     /translation="DDVVDSSKSFVMENFSSYHGTKPGYVDSIQKGIQKPKSGTQGNYD
                     DDWKGFYSTDNKYDAAGYSVDNENPLSGKAGGVVKVTYPGLTKVLALKVDNAETIKKEL
                     GLSLTEPLMEQVGTEEFIKRFGDGASRVVLSLPFAEGSSSVEYINNWEQAKALSVELEI
                     NFETRGKRGQDAMYEYMAQACAGNRVRR"
     intron          6829..6894
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     primer_bind     complement(7042..7061)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    7107..7162
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(7161..7180)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(7507..7526)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7508..7526)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7533..7550)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7533..7549)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7542..7564)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7690..8118
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7777..7796)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7987..8008
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             8462..9256
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             join(9274..9342,9343..10134)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9492..9511)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10305..10893
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10794..10813
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc
       61 gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg
      121 gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca
      181 ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt
      241 tcacacagga aacagctatg accatgatta cgccaagctt cccattggcg ataacttttg
      301 attgagaata tccgaaaagt tttattctga atgagagtgc aaaagtactg gcctgagtct
      361 tttttttgag attgagtttc actcttgttg cccagattgg agtgcaatgg tgcaatcttg
      421 gctctctgcc tcctgggttc aagcgattct cctgcctcag tctcccaagt agctgggatt
      481 agaggcatgt gctaccacat ccagctaata tttagtagag acagggagag gcagcttatt
      541 tttcgtattt agtagagacg gggtttcacc acgttggtgg tctcaaacct ctgacctcag
      601 gtgatccacc tgcctgggcc tccccagtac tgggattaca ggcgtgagcg actgtgccag
      661 gcctgtctga gtctttctca gtgaaactta acagcgctgt tccgtgagct ctgaatatgt
      721 ggataatctt tttggtcagc ttcccagtga ttttgtttct tactaagaga tatgacaata
      781 acttctatac atcactgaat gtgcttgtga gtcacttagt aatttggaga aaatgacctg
      841 ttagaaagaa aaacactgtc ataccaggca ttcgctcaga acgaatggga ttcctggtgt
      901 ggtgcccctc cttgcccggc cgcagcctct gcccaggccc tgctcactgg ggtctttctc
      961 ttgcagtcag taccccagcc tgtggtccgt gagcaacggc gccgtcaccc cgggctccca
     1021 ggcagcagcc gtgtccaacg ggctggggac ccagttcttc cggggctccc ccgcgcacta
     1081 cgcacccctc acccatccgg tcccggtgcc ctcttcctct ggatccccac tgtacgaagg
     1141 ggcggcggcg gccacagaca tcgcggacag ccagtacgac gccgcccaag gccgcctcat
     1201 agcctcatgg acacctgtgt cgccgccttc catggctagc gccactaact tctccctgtt
     1261 gaaacaagca ggggatgtcg aagagaatcc cgggccaatg gtgagcaagg gcgaggaggt
     1321 catcaaagag ttcatgcgct tcaaggtgcg catggagggc tccatgaacg gccacgagtt
     1381 cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg ccaagctgaa
     1441 ggtgaccaag ggcggccccc tgcccttcgc ctgggacatc ctgtcccccc agttcatgta
     1501 cggctccaag gcgtacgtga agcaccccgc cgacatcccc gattacaaga agctgtcctt
     1561 ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggtc tggtgaccgt
     1621 gacccaggac tcctccctgc aggacggcac gctgatctac aaggtgaaga tgcgcggcac
     1681 caacttcccc cccgacggcc ccgtaatgca gaagaagacc atgggctggg aggcctccac
     1741 cgagcgcctg tacccccgcg acggcgtgct gaagggcgag atccaccagg ccctgaagct
     1801 gaaggacggc ggccactacc tggtggagtt caagaccatc tacatggcca agaagcccgt
     1861 gcaactgccc ggctactact acgtggacac caagctggac atcacctccc acaacgagga
     1921 ctacaccatc gtggaacagt acgagcgctc cgagggccgc caccacctgt tcctggggca
     1981 tggcaccggc agcaccggca gcggcagctc cggcaccgcc tcctccgagg acaacaacat
     2041 ggccgtcatc aaagagttca tgcgcttcaa ggtgcgcatg gagggctcca tgaacggcca
     2101 cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa
     2161 gctgaaggtg accaagggcg gccccctgcc cttcgcctgg gacatcctgt ccccccagtt
     2221 catgtacggc tccaaggcgt acgtgaagca ccccgccgac atccccgatt acaagaagct
     2281 gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggtctggt
     2341 gaccgtgacc caggactcct ccctgcagga cggcacgctg atctacaagg tgaagatgcg
     2401 cggcaccaac ttcccccccg acggccccgt aatgcagaag aagaccatgg gctgggaggc
     2461 ctccaccgag cgcctgtacc cccgcgacgg cgtgctgaag ggcgagatcc accaggccct
     2521 gaagctgaag gacggcggcc actacctggt ggagttcaag accatctaca tggccaagaa
     2581 gcccgtgcaa ctgcccggct actactacgt ggacaccaag ctggacatca cctcccacaa
     2641 cgaggactac accatcgtgg aacagtacga gcgctccgag ggccgccacc acctgttcct
     2701 gtacggcatg gacgagctgt acaagtaggc ggccgcgact ctagatcata atcagccata
     2761 ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga
     2821 aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca
     2881 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     2941 gtggtttgtc caaactcatc aatgtatctt aaggcgttag gaattccgat catattcaat
     3001 aacccttaat ataacttcgt ataatgtatg ctatacgaag ttattaggtc tgaagaggag
     3061 tttacgtcca gccaagctaa ttctaccggg taggggaggc gcttttccca aggcagtctg
     3121 gagcatgcgc tttagcagcc ccgctgggca cttggcgcta cacaagtggc ctctggcctc
     3181 gcacacattc cacatccacc ggtaggcgcc aaccggctcc gttctttggt ggccccttcg
     3241 cgccaccttc tactcctccc ctagtcagga agttcccccc cgccccgcag ctcgcgtcgt
     3301 gcaggacgtg acaaatggaa gtagcacgtc tcactagtct cgtgcagatg gacagcaccg
     3361 ctgagcaatg gaagcgggta ggcctttggg gcagcggcca atagcagctt tgctccttcg
     3421 ctttctgggc tcagaggctg ggaaggggtg ggtccggggg cgggctcagg ggcgggctca
     3481 ggggcggggc gggcgcccga aggtcctccg gaggcccggc attctgcacg cttcaaaagc
     3541 gcacgtctgc cgcgctgttc tcctcttcct catctccggg cctttcgacc tgcagccaat
     3601 atgggatcgg ccattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag
     3661 aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc
     3721 cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg
     3781 aatgaactgc aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc
     3841 gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg
     3901 ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct
     3961 gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg
     4021 aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat
     4081 ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc
     4141 atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg
     4201 gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc
     4261 tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct
     4321 gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat
     4381 cgccttcttg acgagttctt ctgaggggat ccgctgtaag tctgcagaaa ttgatgatct
     4441 attaaacaat aaagatgtcc actaaaatgg aagtttttcc tgtcatactt tgttaagaag
     4501 ggtgagaaca gagtacctac attttgaatg gaaggattgg agctacgggg gtgggggtgg
     4561 ggtgggatta gataaatgcc tgctctttac tgaaggctct ttactattgc tttatgataa
     4621 tgtttcatag ttggatatca taatttaaac aagcaaaacc aaattaaggg ccagctcatt
     4681 cctcccactc atgatctata gatccctcga tcgagatccg gaacccttaa tataacttcg
     4741 tataatgtat gctatacgaa gttattaggt ccctcgaaga ggggcagcca ggcccaggtc
     4801 ctggaggatg cagggacttt gtcgtggcag ccagtggtga ctggagtgac ctactaggtg
     4861 cacagtggca gtctcgcgga ttaagtaagg aaatgcagcc tcactaactt tcttttcaaa
     4921 gcggaggagg agcacagccg cgctgttccc agagccccag cacccgctca caccggcagc
     4981 agcggtgcgg tccccagtgg tgtacaggtg aatccaccga cggccaacct gattcacagg
     5041 caaaaacctt tgccagggtc cagaaactct ttttgatttg ttctcatacg atgtattggc
     5101 aactctggcg caccagaatt tgtaaactcc accggccctt ctttagtgag ataaaaagca
     5161 cacttttaat ctttttcctt gttgctttca agtagttaga gttgacctgt taaggacaga
     5221 ataaaatcat agttgagggc agcaggtttt agctgaattg gaaatttgac tgctctgccc
     5281 gctagaatgt gtgtattttg agcatatgta gacaacttct tgtgttgtta aactgtaact
     5341 gtttcatatt tttcttttga caaaatagcc aaagacaatc agcagaaagc attttctgca
     5401 aaataaaggc aatatgcaaa acgtgattgg tccggttatt agtcaacccg ctccgtttgt
     5461 gggtattcac tgtttattac agctggtggt aaagacctta tccccaaaac gcactttact
     5521 ctaatacaga ctttaatact gtatctagtt ggttgcaaaa ctggaagtga aattttatag
     5581 acttagtgct aattgccaca acaatttaaa agatggtaaa tactttcgaa gagatttgct
     5641 caacactgaa tcttgggtga cgtggataaa gacggaacct agaacttact gacgtcatca
     5701 gcatgaatgt gggatgtcca cccgattcaa gctcaagtac ctacatgaac taaactgata
     5761 caaagttatc aatgtaaagt gcagttacca tagtactttg aaaagaactt tggtaaatat
     5821 ctttctggct gcgttttctg aaaagcgttt tgctttagtt ctgtttagaa ctgcagtggt
     5881 gccagactcg agcagtgtgg ttttcaagag gaagcaaaaa gcctctccac ccaggcctgg
     5941 aatgtttcca cccaatgtcg agcagtgtgg ttttgcaaga ggaagcaaaa agcctctcca
     6001 cccaggcctg gaatgtttcc acccaatgtc gagcaaaccc cgcccagcgt cttgtcattg
     6061 gcgaattcga acacgcagat gcagtcgggg cggcgcggtc ccaggtccac ttcgcatatt
     6121 aaggtgacgc gtgtggcctc gaacaccgag cgaccctgca ggtcctcgcc atggatcctg
     6181 atgatgttgt tgattcttct aaatcttttg tgatggaaaa cttttcttcg taccacggga
     6241 ctaaacctgg ttatgtagat tccattcaaa aaggtataca aaagccaaaa tctggtacac
     6301 aaggaaatta tgacgatgat tggaaagggt tttatagtac cgacaataaa tacgacgctg
     6361 cgggatactc tgtagataat gaaaacccgc tctctggaaa agctggaggc gtggtcaaag
     6421 tgacgtatcc aggactgacg aaggttctcg cactaaaagt ggataatgcc gaaactatta
     6481 agaaagagtt aggtttaagt ctcactgaac cgttgatgga gcaagtcgga acggaagagt
     6541 ttatcaaaag gttcggtgat ggtgcttcgc gtgtagtgct cagccttccc ttcgctgagg
     6601 ggagttctag cgttgaatat attaataact gggaacaggc gaaagcgtta agcgtagaac
     6661 ttgagattaa ttttgaaacc cgtggaaaac gtggccaaga tgcgatgtat gagtatatgg
     6721 ctcaagcctg tgcaggaaat cgtgtcaggc gatctctttg tgaaggaacc ttacttctgt
     6781 ggtgtgacat aattggacaa actacctaca gagatttaaa gctctaaggt aaatataaaa
     6841 tttttaagtg tataatgtgt taaactactg attctaattg tttgtgtatt ttagattcca
     6901 acctatggaa ctgatgaatg ggagcagtgg tggaatgcag atccactaga gtcgaagcgg
     6961 cttcactcct caggtgcagg ctgcctatca gaaggtggtg gctggtgtgg ccaatgccct
     7021 ggctcacaaa taccactgag atctttttcc ctctgccaaa aattatgggg acatcatgaa
     7081 gccccttgag catctgactt ctggctaata aaggaaattt attttcattg caatagtgtg
     7141 ttggaatttt ttgtgtctct cactcggaag gacatatggg agggcaaatc atttaaaaca
     7201 tcagaatgag tatttggttt agagtttggc aacatatgcc catatgctgg ctgccatgaa
     7261 caaaggttgg ctataaagag gtcatcagta tatgaaacag ccccctgctg tccattcctt
     7321 attccataga aaagccttga cttgaggtta gatttttttt atattttgtt ttgtgttatt
     7381 tttttcttta acatccctaa aattttcctt acatgtttta ctagccagat ttttcctcct
     7441 ctcctgacta ctcccagtca tagctgtccc tcttctctta tggagatctc tagagggccc
     7501 aattcgccct atagtgagtc gtattacaat tcactggccg tcgttttaca acgtcgtgac
     7561 tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc
     7621 tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat
     7681 ggcgaatgga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
     7741 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
     7801 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
     7861 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac
     7921 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
     7981 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt
     8041 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
     8101 aaaaatttaa cgcgaatttt aacaaaattc agggcgcaag ggctgctaaa ggaagcggaa
     8161 cacgtagaaa gccagtccgc agaaacggtg ctgaccccgg atgaatgtca gctactgggc
     8221 tatctggaca agggaaaacg caagcgcaaa gagaaagcag gtagcttgca gtgggcttac
     8281 atggcgatag ctagactggg cggttttatg gacagcaagc gaaccggaat tgccagctgg
     8341 ggcgccctct ggtaaggttg ggaagccctg caaagtaaac tggatggctt tcttgccgcc
     8401 aaggatctga tggcgcaggg gatcaagatc tgatcaagag acaggatgag gatcgtttcg
     8461 catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt
     8521 cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc
     8581 agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact
     8641 gcaggacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt
     8701 gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca
     8761 ggatctcctg tcatcccacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat
     8821 gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg
     8881 catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga
     8941 agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gcatgcccga
     9001 cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa
     9061 tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga
     9121 catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt
     9181 cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct
     9241 tgacgagttc ttctgaattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     9301 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     9361 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     9421 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     9481 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
     9541 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     9601 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     9661 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     9721 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     9781 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     9841 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     9901 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     9961 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
    10021 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
    10081 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
    10141 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
    10201 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
    10261 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
    10321 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
    10381 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
    10441 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
    10501 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
    10561 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
    10621 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
    10681 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
    10741 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
    10801 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
    10861 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
    10921 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
    10981 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccg
//
