LOCUS       sequence-37705-e         897 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 897)
  AUTHORS   Calvisi DF, Wang C, Ho C, Ladu S, Lee SA, Mattu S, Destefanis G, 
            Delogu S, Zimmermann A, Ericsson J, Brozzetti S, Staniscia T, Chen 
            X, Dombrowski F, Evert M
  TITLE     Increased lipogenesis, induced by AKT-mTORC1-RPS6 signaling, 
            promotes development of human hepatocellular carcinoma.
  JOURNAL   Gastroenterology. 2011 Mar;140(3):1071-83. Epub 2010 Dec 11.
  PUBMED    21147110
REFERENCE   2  (bases 1 to 897)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Gastroenterology."; date: "2011-03"; volume: "140(3)"; pages: 
            "1071-83. Epub 2010 Dec 11"
COMMENT     Sequence Label: pT3-myr-AKT-HA
FEATURES             Location/Qualifiers
     source          1..897
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     26..45
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        26..44
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    123..147
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             168..188
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSK"
ORIGIN
        1 gtgtcgtgag gaattagctt ggtactaata cgactcacta tagggagacc caagctggct
       61 aggtaagctt ggtaccgagc tcggatccac tagtccagtg tggtggaatt ctgcagatat
      121 caacaagttt gtacaaaaaa gcaggctccg cggccgcccc cttcaccatg gggagcagca
      181 agagcaagcc caagtctaga atgaacgacg tagccattgt gaaggagggc tggctgcaca
      241 aacgagggga atatattaaa acctggcggc cacgctactt cctcctcaag aacgatggca
      301 cctttattgg ctacaaggaa cggcctcagg atgtggatca gcgagagtcc ccactcaaca
      361 acttctcagt ggcacaatgc cagctgatga agacagagcg gccaaggccc aacaccttta
      421 tcatccgctg cctgcagtgg accacagtca ttgagcgcac cttccatgtg gaaacgcctg
      481 aggagcggga agaatgggcc accgccattc agactgtggc cgatggactc aagaggcagg
      541 aagaagagac gatggacttc cgatcaggct cacccagtga caactcaggg gctgaagaga
      601 tggaggtgtc cctggccaag cccaagcacc gtgtgaccat gaacgagttt gagtacctga
      661 aactactggg caagggcacc tttgggaaag tgattctggt gaaagagaag gccacaggcc
      721 gctactatgc catgaagatc ctcaagaagg aggtcatcgt cgccaaggat gaggttgccc
      781 acacgcttac tgagaaccgt gtcctgcaga actctaggca tcccttcctt acggccctca
      841 agtactcatt ccagacccac gaccgcctct gctttgtcat ggagtatgcc aacgggg
//
