LOCUS       sequence-377051-        7271 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  aadA.co-TaadA-PJ23111-eYFP.co between homology arms for pilA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7271)
  AUTHORS   Jacob M. Hilzinger, Skyler Friedline, Divya Sivanandan, Ya-Fang 
            Cheng, Shunsuke Yamazaki, Douglas S. Clark, Jeffrey M. Skerker, Adam
            P. Arkin
  TITLE     Acetaminophen production in the edible, filamentous cyanobacterium 
            Arthrospira platensis
  JOURNAL   bioRxiv
REFERENCE   2  (bases 1 to 7271)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv"
COMMENT     Sequence Label: pJMH140
FEATURES             Location/Qualifiers
     source          1..7271
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     364..386
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     378..395
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2452..2486
                     /label=BBa_J23111
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.58"
     mobile_element  2499..3266
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     RBS             3300..3311
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     primer_bind     complement(5050..5066)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5050..5066)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    5074..5090
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5098..5104,5105..5122,5123..5128))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5143..5164
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5281..5298)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5452..6040)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5532..5551)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6211..7002,7003..7071))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6834..6853
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7072..7176)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7244..7262
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtggaat cttgtgcgaa ttgaggtaac
      421 ctcagatcct atccttgagg ttgattgcgt atttctgggg aaaacggagt ttcctcggcc
      481 ctataatgaa actgataagg aggaagattg atgcgaaaat acatcatttt tcgggctgat
      541 aagggtgaac ctggttggaa agaacgtaaa ctccaacata cccagggttt aactagaatt
      601 ttggctgaac atttcgactc ttctgatcaa cctattcctg agttgggata tcgaccgagg
      661 gagtttattc gtattgatgg gttgcataat cccactgaac atggctacag cacccattat
      721 cgtcagggtg actgggaagt aactagggta gaaagttact ctcctgatgt gtctatggct
      781 gagtttgata tgatcgtgat ttgcttttgc aaatatagcc ctgttaatgg tcctgtgcaa
      841 cctatgccag agcgtcgagt gtctgtagat gggttcgctg gggatgagaa gcggttggag
      901 caatggcacg agtcccaaaa gaaaccgact gaggtttagt tggtccccgg tttgcttatt
      961 tgactagcca gattcgccac gacaacagaa ccgccagcag ggaagagggg aatagaaccg
     1021 cgaaaagagc gatcgcattg gtttcgggaa tggcgaaatt tggcccgcca tatttgatca
     1081 gtaaggatag ccctagggat aggatcagga ttttggccac caataacagt ttgttgtcca
     1141 tttatatata ggatgcgatg ggatgcgatc gcctatattt taccaattgg tggcggcctt
     1201 aaccccctaa gccctatgtc tgacacccag tcaccccaac cgccttagtc ggtattcccc
     1261 actgggtggg cgatacacga tttcccggaa aaattgaaaa aaaattttgt ggcaggtgat
     1321 atgatttgcg atcgaggtgg gtaagttaga actacagaaa acaaataacc tcatttcacc
     1381 ccgaggagaa cgaattatga gagaagctgt gatcgccgag gtttcaacac agttatccga
     1441 agttgtaggg gttatcgaac gacatttaga gccaacctta ttggcggtac acttgtatgg
     1501 ttcagctgtc gatggaggcc ttaaacccca ttccgatatt gaccttctgg tcactgtgac
     1561 agttagactt gatgaaacca ctagaagggc cctgatcaat gacttattag aaacttctgc
     1621 aagtcctggt gaaagtgaga tattgagggc ggtagaagtc acaattgtcg tgcacgatga
     1681 cattatcccc tggagatacc ccgctaaaag ggaactgcaa tttggagagt ggcagcgaaa
     1741 cgatattcta gcaggcatat tcgaacctgc gactatcgat attgaccttg ctatactgtt
     1801 gacaaaagcc cgcgagcatt ctgtggcact agttggaccg gctgccgaag aattatttga
     1861 tcctgttccg gaacaagacc ttttcgaagc actgaatgag accctaactc tctggaacag
     1921 tccacccgat tgggccggag acgagcggaa tgttgtatta accttgagcc gaatctggta
     1981 ttcggcggtg actggcaaaa tagctccaaa agatgttgca gcggactggg ctatggaaag
     2041 gttacctgca caatatcagc cggttatact agaggcacgg caagcgtacc tcgggcagga
     2101 agaggatcgc ttagcttcaa gagccgacca attggaagag ttcgtacact acgtcaaagg
     2161 ggaaattacc aaagtcgtgg gtaaataatg tctaacaatt cgttcaagcc gacgccgctt
     2221 cgcggcgcgg cttaactcaa gcgttagatg cactaagcac ataattgctc acagccaaac
     2281 tatcaggtca agtctgcttt tattattttt aagcgtgcat aataagccct acacaaattg
     2341 ggagatatat catgaaaggc tggctttttc ttgttatcgc aatagttggc gaagtaatcg
     2401 caacatccgc attaaaatct agcgagggct ttactaagct gatccgggga gttgacggct
     2461 agctcagtcc taggtatagt gctagctatt gtgagcgggg taatgactcc aacttattga
     2521 tagtgtttta tgttcagata atgcccgatg actttgtcat gcagctccac cgattttgag
     2581 aacgacagcg acttccgtcc cagccgtgcc aggtgctgcc tcagattcag gttatgccgc
     2641 tcaattcgct gcgtatatcg cttgctgatt acgtgcagct ttcccttcag gcgggattca
     2701 tacagcggcc agccatccgt catccatatc accacgtcaa agggtgacag caggctcata
     2761 agacgcccca gcgtcgccat agtgcgttca ccgaatacgt gcgcaacaac cgtcttccgg
     2821 agactgtcat acgcgtaaaa cagccagcgc tggcgcgatt tagccccgac atagccccac
     2881 tgttcgtcca tttccgcgca gacgatgacg tcactgcccg gctgtatgcg cgaggttacc
     2941 gactgcggcc tgagtttttt aagtgacgta aaatcgtgtt gaggccaacg cccataatgc
     3001 gggctgttgc ccggcatcca acgccattca tggccatatc aatgattttc tggtgcgtac
     3061 cgggttgaga agcggtgtaa gtgaactgca gttgccatgt tttacggcag tgagagcaga
     3121 gatagcgctg atgtccggcg gtgcttttgc cgttacgcac caccccgtca gtagctgaac
     3181 aggagggaca gctgatagaa acagaagcca ctggagcacc tcaaaaacac catcatacac
     3241 taaatcagta agttggcagc atcacctgtg agcggataac aatttcacac atactagaga
     3301 aagaggagaa atactaatgg tatctaaagg tgaagagctg tttaccggag ttgtacccat
     3361 tttagttgaa ttggatggcg acgtaaacgg gcataaattc tcagtctccg gggaaggaga
     3421 gggagatgca acttatggca aactaacact caaatttatc tgtaccactg ggaaactacc
     3481 agtcccctgg cctactctcg tgacaacctt tggctatggg ttacaatgct tcgcaaggta
     3541 tccagatcac atgaaacagc acgacttttt caaatcagcg atgcctgaag gctacgttca
     3601 agagcgaaca atatttttca aagatgacgg aaactacaaa acaagagcgg aagttaggtt
     3661 tgagggtgat accctggtaa accgcatcga attgaaagga atcgatttca aagaggacgg
     3721 taacatactt ggacataaac tggaatacaa ctacaactcg cacaacgtct acatcatggc
     3781 tgataaacag aaaaatggta tcaaagtgaa cttcaaaatc agacataaca tcgaggatgg
     3841 gtcagttcag ttagccgacc actaccaaca gaatactccg atcggtgatg gaccggtttt
     3901 attgccagac aaccattatc tttcttacca gagtgctctg agcaaagatc ctaatgaaaa
     3961 acgggaccac atggtccttc tggagtttgt gaccgctgcc ggcattactc tagggatgga
     4021 tgaactctat aaataacaga taatctagca gtttaggttt cagatggagt cagctagggt
     4081 ctttaacccc atcgtcaact ataagatgga gagatggcat acggtgacac tgttaaagcc
     4141 ataactatat tgctcttgtt gataaaatca gaaactgagg ccacttgaga gtattgtaaa
     4201 taggtagtac agtagtgcta cctattttgt tttaaaataa ctaattgagt ttatcgctgg
     4261 gactatggtt gataatcaga tgtcggaaaa gcagaatatt ttatcagcag aagctgatat
     4321 ttacttgcat aaaagggatt atcaacaagc agccatttta tatgaacaag ccatcaatct
     4381 caatcccgac tataaatata attactggaa actggggtta accctgctgc tacaggagcg
     4441 ggaggaagaa gcccagacca cctggctggt ggcgatgagt gatggagacc cagaacagat
     4501 tgaccagtgg acgatagact tactcgaggt gctatcatca gaagccgatg cacgaacctc
     4561 cgccgaagac tattccgtgg cttgggggat tcgccaacac attttagaaa taaaccccca
     4621 tggtattgat aatatattaa agttaattga tttgtcaatt accctgacaa cctataccgg
     4681 aaatgagatc gcagaatatg gactggttcc cttactgcaa caggagtcca tcaatgtcaa
     4741 tccagattta ctactgcacc tgtggaaaaa actcctagtt gtagatgcaa ttaatcccct
     4801 atctctggaa ttgggagagg cggtgattaa ccatcttggt gagcaaccgg agtttgtcac
     4861 tagcttaatc cctaaattat acgatattgc ctattccttt ggtgaaatag gtatagccaa
     4921 gggctatgga gagttgttgt tgaacctggc accgcatcat caagagttgc tgagggctct
     4981 ggctcaattt tgtattcaat taaccgagta tgaccatggc attgaatatg ccagactggc
     5041 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     5101 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     5161 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
     5221 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     5281 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     5341 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     5401 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     5461 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     5521 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     5581 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     5641 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     5701 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     5761 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     5821 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     5881 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     5941 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     6001 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     6061 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     6121 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     6181 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     6241 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     6301 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     6361 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     6421 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     6481 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     6541 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     6601 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     6661 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     6721 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     6781 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     6841 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     6901 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     6961 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     7021 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     7081 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     7141 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
     7201 tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag
     7261 gccctttcgt c
//
