LOCUS       sequence-37707-e         670 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses humanized ChR2 fused to tdTomato for optogenetic 
            activation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 670)
  AUTHORS   Mao T, Kusefoglu D, Hooks BM, Huber D, Petreanu L, Svoboda K
  TITLE     Long-Range Neuronal Circuits Underlying the Interaction between 
            Sensory and Motor Cortex.
  JOURNAL   Neuron. 2011 Oct 6;72(1):111-23.
  PUBMED    21982373
REFERENCE   2  (bases 1 to 670)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron."; 
            date: "2011-10-6"; volume: "72(1)"; pages: "111-23"
COMMENT     Sequence Label: AAV-CAG-hChR2_tdTomato
FEATURES             Location/Qualifiers
     source          1..670
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(164..187)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(362..381)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     631..649
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 atccagaggt tgattatcga taagcttgat atcgaattcg aatcgactag tcgcgagctc
       61 cactcgagtg gatcccctta cttgtacagc tcgtccatgc cgtacaggaa caggtggtgg
      121 cggccctcgg agcgctcgta ctgttccacg atggtgtagt cctcgttgtg ggaggtgatg
      181 tccagcttgg tgtccacgta gtagtagccg ggcagttgca cgggcttctt ggccatgtag
      241 atggtcttga actccaccag gtagtggccg ccgtccttca gcttcagggc ctggtggatc
      301 tcgcccttca gcacgccgtc gcgggggtac aggcgctcgg tggaggcctc ccagcccatg
      361 gtcttcttct gcattacggg gccgtcgggg gggaagttgg tgccgcgcat cttcaccttg
      421 tagatcagcg tgccgtcctg cagggaggag tcctgggtca cggtcaccag accgccgtcc
      481 tcgaagttca tcacgcgctc ccacttgaag ccctcgggga aggacagctt cttgtaatcg
      541 gggatgtcgg cggggtgctt cacgtacgcc ttggagccgt acatgaactg gggggacagg
      601 atgtcccagg cgaagggcag ggggccgccc ttggtcacct tcagcttggc ggtctgggtg
      661 ccctcgtagg
//
